DE69815384D1 - Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems - Google Patents

Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Info

Publication number
DE69815384D1
DE69815384D1 DE69815384T DE69815384T DE69815384D1 DE 69815384 D1 DE69815384 D1 DE 69815384D1 DE 69815384 T DE69815384 T DE 69815384T DE 69815384 T DE69815384 T DE 69815384T DE 69815384 D1 DE69815384 D1 DE 69815384D1
Authority
DE
Germany
Prior art keywords
new method
host cell
homologous recombination
recombination system
cloning dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69815384T
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English (en)
Other versions
DE69815384T2 (de
Inventor
Francis Stewart
Youming Zhang
Frank Buchholz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
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Europaisches Laboratorium fuer Molekularbiologie EMBL
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Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Publication of DE69815384D1 publication Critical patent/DE69815384D1/de
Application granted granted Critical
Publication of DE69815384T2 publication Critical patent/DE69815384T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69815384T 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems Expired - Lifetime DE69815384T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97121462 1997-12-05
EP98118756 1998-10-05
PCT/EP1998/007945 WO1999029837A2 (en) 1997-12-05 1998-12-07 Novel dna cloning method relying on the e. coli rece/rect recombination system

Publications (2)

Publication Number Publication Date
DE69815384D1 true DE69815384D1 (de) 2003-07-10
DE69815384T2 DE69815384T2 (de) 2003-12-04

Family

ID=26145961

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69841340T Expired - Lifetime DE69841340D1 (de) 1997-12-05 1998-12-07 Neue Methode zur Klonierung DNS unter Anwendung des E. coli RecE/RecT Rekombinationssystems
DE69815384T Expired - Lifetime DE69815384T2 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69841340T Expired - Lifetime DE69841340D1 (de) 1997-12-05 1998-12-07 Neue Methode zur Klonierung DNS unter Anwendung des E. coli RecE/RecT Rekombinationssystems

Country Status (11)

Country Link
US (5) US6509156B1 (de)
EP (2) EP1291420B1 (de)
JP (1) JP4139561B2 (de)
AT (2) ATE449844T1 (de)
AU (1) AU752105B2 (de)
CA (2) CA2312474C (de)
DE (2) DE69841340D1 (de)
DK (1) DK1034260T3 (de)
ES (2) ES2201567T3 (de)
PT (1) PT1034260E (de)
WO (1) WO1999029837A2 (de)

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Also Published As

Publication number Publication date
WO1999029837B1 (en) 1999-09-16
ATE242320T1 (de) 2003-06-15
US7736851B2 (en) 2010-06-15
EP1034260A2 (de) 2000-09-13
AU1877199A (en) 1999-06-28
JP2002503448A (ja) 2002-02-05
EP1291420A1 (de) 2003-03-12
US6509156B1 (en) 2003-01-21
ATE449844T1 (de) 2009-12-15
WO1999029837A3 (en) 1999-07-22
US20030036198A1 (en) 2003-02-20
CA2312474A1 (en) 1999-06-17
PT1034260E (pt) 2003-10-31
US6787316B2 (en) 2004-09-07
EP1291420B1 (de) 2009-11-25
US20120208277A1 (en) 2012-08-16
US20100317061A1 (en) 2010-12-16
CA2759882A1 (en) 1999-06-17
US20040203057A1 (en) 2004-10-14
DE69841340D1 (de) 2010-01-07
DK1034260T3 (da) 2003-09-29
ES2201567T3 (es) 2004-03-16
ES2337027T3 (es) 2010-04-20
CA2312474C (en) 2012-07-03
JP4139561B2 (ja) 2008-08-27
AU752105B2 (en) 2002-09-05
EP1034260B1 (de) 2003-06-04
DE69815384T2 (de) 2003-12-04
WO1999029837A2 (en) 1999-06-17

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