ATE449844T1 - Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems - Google Patents

Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Info

Publication number
ATE449844T1
ATE449844T1 AT02021915T AT02021915T ATE449844T1 AT E449844 T1 ATE449844 T1 AT E449844T1 AT 02021915 T AT02021915 T AT 02021915T AT 02021915 T AT02021915 T AT 02021915T AT E449844 T1 ATE449844 T1 AT E449844T1
Authority
AT
Austria
Prior art keywords
new method
host cell
homologous recombination
recombination system
cloning dna
Prior art date
Application number
AT02021915T
Other languages
English (en)
Inventor
Francis Stewart
Youming Zhang
Frank Buchholz
Original Assignee
Europ Lab Molekularbiolog
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog filed Critical Europ Lab Molekularbiolog
Application granted granted Critical
Publication of ATE449844T1 publication Critical patent/ATE449844T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02021915T 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems ATE449844T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97121462 1997-12-05
EP98118756 1998-10-05

Publications (1)

Publication Number Publication Date
ATE449844T1 true ATE449844T1 (de) 2009-12-15

Family

ID=26145961

Family Applications (2)

Application Number Title Priority Date Filing Date
AT98963541T ATE242320T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
AT02021915T ATE449844T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT98963541T ATE242320T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Country Status (11)

Country Link
US (5) US6509156B1 (de)
EP (2) EP1291420B1 (de)
JP (1) JP4139561B2 (de)
AT (2) ATE242320T1 (de)
AU (1) AU752105B2 (de)
CA (2) CA2759882A1 (de)
DE (2) DE69841340D1 (de)
DK (1) DK1034260T3 (de)
ES (2) ES2201567T3 (de)
PT (1) PT1034260E (de)
WO (1) WO1999029837A2 (de)

Families Citing this family (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821759B1 (en) 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US6156574A (en) 1997-06-23 2000-12-05 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
NZ520579A (en) * 1997-10-24 2004-08-27 Invitrogen Corp Recombinational cloning using nucleic acids having recombination sites and methods for synthesizing double stranded nucleic acids
DK1034260T3 (da) * 1997-12-05 2003-09-29 Europ Lab Molekularbiolog Hidtil ukendt DNA-kloningsmetode under anvendelse af E.coli-RECE/RECT-rekombinationssystemet
AUPP684998A0 (en) * 1998-10-30 1998-11-26 Murdoch Institute for Research into Birth Defects Limited, The A method of recombination and agents useful for same
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6709852B1 (en) 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
CA2370887A1 (en) * 1999-06-25 2001-01-04 Benjamin J. Metcalf Production of the lipidated form of the peptidoglycan-associated lipoproteins of gram-negative bacteria
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
AU6229800A (en) * 1999-07-20 2001-02-05 Rockefeller University, The Conditional homologous recombination of large genomic vector inserts
WO2001034810A2 (en) 1999-11-09 2001-05-17 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation
AU2001283377B2 (en) * 2000-08-14 2007-09-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Enhanced homologous recombination mediated by lambda recombination proteins
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1399546A2 (de) * 2001-02-09 2004-03-24 Gene Bridges GmbH Rekombinationsmethode
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
EP1409702A1 (de) * 2001-07-20 2004-04-21 Youming Zhang Verbesserte rect oder recet klonierungs- und subklonierungs-methode
US20030082591A1 (en) * 2001-07-24 2003-05-01 Donald Awrey Methods for gene disruption and uses thereof
US7338800B2 (en) * 2002-01-16 2008-03-04 Baylor College Of Medicine In vivo gene transfer
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US20060270043A1 (en) * 2002-01-23 2006-11-30 Blattner Frederick R Bacteria with reduced genome
US8039243B2 (en) * 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8119365B2 (en) * 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
US7518033B2 (en) * 2002-08-02 2009-04-14 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
DE10236001A1 (de) 2002-08-06 2004-02-19 Icon Genetics Ag Transformation von Plastiden unter Verwendung modularer Vektoren
US7303906B2 (en) * 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
US7521242B2 (en) * 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
EP1687323A4 (de) 2003-08-08 2009-08-12 Life Technologies Corp Verfahren und zusammensetzungen zum nahtlosen klonieren von nukleinsäuremolekülen
DE602004027538D1 (de) * 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20060127920A1 (en) * 2004-02-27 2006-06-15 President And Fellows Of Harvard College Polynucleotide synthesis
JP2005245320A (ja) * 2004-03-04 2005-09-15 Aisin Seiki Co Ltd 核酸組換え方法、宿主細胞、及び、発現ベクター
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
JP2008523786A (ja) * 2004-10-18 2008-07-10 コドン デバイシズ インコーポレイテッド 高忠実度合成ポリヌクレオチドのアセンブリ方法
GB0423755D0 (en) * 2004-10-26 2004-11-24 Gene Bridges Gmbh Methods for heterologus expression of secondary metabolites
US20080051326A1 (en) * 2004-11-12 2008-02-28 Alexander Dylan C Antiinfective Lipopeptides
WO2006127423A2 (en) * 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
US20070004041A1 (en) * 2005-06-30 2007-01-04 Codon Devices, Inc. Heirarchical assembly methods for genome engineering
US20070243617A1 (en) * 2005-10-13 2007-10-18 Holt Robert A Modular genomes for synthetic biology and metabolic engineering
KR100681815B1 (ko) 2005-12-02 2007-02-12 한국생명공학연구원 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법.
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
JP5551432B2 (ja) * 2006-05-25 2014-07-16 サンガモ バイオサイエンシーズ， インコーポレイテッド 遺伝子不活性化のための方法と組成物
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US9683252B2 (en) 2007-05-17 2017-06-20 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
GB0803109D0 (en) * 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
US8841094B2 (en) 2008-03-07 2014-09-23 National University Corporation University Of Toyama Homologous recombination method, cloning method, and kit
US20100062495A1 (en) 2008-09-10 2010-03-11 Genscript Corporation Homologous recombination-based DNA cloning methods and compositions
GB0909660D0 (en) 2009-06-04 2009-07-22 Gene Bridges Gmbh Method of altering nucleic acids
PT2421357E (pt) 2009-07-08 2013-04-18 Kymab Ltd Modelos animais e moléculas terapêuticas
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
CA2781400A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an organic acid and/or related chemicals
GB0922108D0 (en) 2009-12-17 2010-02-03 Gene Bridges Gmbh Heterologous hosts
WO2011080505A2 (en) 2009-12-28 2011-07-07 Bigdna Ltd Method of growing phage for commercial use
JP2011217736A (ja) * 2010-03-26 2011-11-04 Aichi Medical Univ 組換えクラミジア及びその製造方法
CN103097400B (zh) 2010-05-27 2015-12-16 细胞制药有限公司 新的7α-羟类固醇脱氢酶敲除突变体及其用途
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2582230A1 (de) 2010-06-17 2013-04-24 Kymab Limited Tiermodelle und therapeutische moleküle
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP6118725B2 (ja) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． 核酸合成のための方法およびデバイス
BR112013024288A2 (pt) 2011-03-22 2016-08-16 Opx Biotechnologies Inc método para a produção de ácido graxo ou derivado de ácido graxo, organismo geneticamente modificado e produto
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
EP3128009B1 (de) 2011-09-19 2020-07-29 Kymab Limited Massgeschneiderte antikörper, variable domänen und ketten für menschliche verwendung
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
CN103160495A (zh) * 2011-12-15 2013-06-19 张力军 一种非连接依赖性的快速克隆方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN102634534A (zh) * 2012-03-30 2012-08-15 深圳市中联生物科技开发有限公司 基于同源重组的核酸分子克隆方法及相关试剂盒
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130288320A1 (en) 2012-04-27 2013-10-31 Bioamber Inc. Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
CN102676470B (zh) * 2012-05-29 2014-04-16 吴江近岸蛋白质科技有限公司 一种用于快速基因克隆的酶混合物及制备方法、包含该酶混合物的试剂盒
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
US20150353940A1 (en) * 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
AU2013299414A1 (en) 2012-08-10 2015-03-26 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
SI2959006T1 (sl) 2013-02-22 2018-10-30 F. Hoffmann-La Roche Ag Uporaba prokariontskega seva, ozdravljenega aminokislinske avksotrofije, za proizvodnjo rekombinantnega polipeptida
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (ja) 2013-07-19 2019-11-06 カーギル インコーポレイテッド 脂肪酸及び脂肪酸誘導体の製造のための微生物及び方法
GB2528177B (en) 2014-06-11 2019-08-28 Univ Duke Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP2993228B1 (de) 2014-09-02 2019-10-09 Cargill, Incorporated Herstellung von fettsäureestern
CN107532129B (zh) 2014-12-09 2022-09-13 生命技术公司 高效小体积核酸合成
CN104630178A (zh) * 2015-01-28 2015-05-20 苏州神洲基因有限公司 一种混合蛋白酶体系、包含该混合蛋白酶体系的试剂盒及其使用方法
CN107750276A (zh) 2015-04-09 2018-03-02 基因组股份公司 用于改进巴豆醇生产的工程化微生物和方法
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
SI3645719T1 (sl) 2017-06-30 2022-07-29 Inscripta, Inc., Postopki, moduli, instrumenti in sistemi za avtomatizirano obdelavo celic
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
CN111372650A (zh) 2017-09-30 2020-07-03 因思科瑞普特公司 流通式电穿孔仪器
US10435662B1 (en) 2018-03-29 2019-10-08 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
AU2019363487A1 (en) 2018-08-30 2021-04-15 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4963487A (en) * 1985-01-03 1990-10-16 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK1034260T3 (da) * 1997-12-05 2003-09-29 Europ Lab Molekularbiolog Hidtil ukendt DNA-kloningsmetode under anvendelse af E.coli-RECE/RECT-rekombinationssystemet

Also Published As

Publication number Publication date
US20040203057A1 (en) 2004-10-14
US20030036198A1 (en) 2003-02-20
JP4139561B2 (ja) 2008-08-27
US6787316B2 (en) 2004-09-07
EP1291420A1 (de) 2003-03-12
EP1034260A2 (de) 2000-09-13
AU752105B2 (en) 2002-09-05
EP1291420B1 (de) 2009-11-25
ATE242320T1 (de) 2003-06-15
US20120208277A1 (en) 2012-08-16
DE69815384D1 (de) 2003-07-10
CA2312474A1 (en) 1999-06-17
WO1999029837A3 (en) 1999-07-22
CA2759882A1 (en) 1999-06-17
EP1034260B1 (de) 2003-06-04
ES2201567T3 (es) 2004-03-16
US20100317061A1 (en) 2010-12-16
DE69815384T2 (de) 2003-12-04
US7736851B2 (en) 2010-06-15
PT1034260E (pt) 2003-10-31
WO1999029837B1 (en) 1999-09-16
JP2002503448A (ja) 2002-02-05
AU1877199A (en) 1999-06-28
WO1999029837A2 (en) 1999-06-17
ES2337027T3 (es) 2010-04-20
US6509156B1 (en) 2003-01-21
CA2312474C (en) 2012-07-03
DK1034260T3 (da) 2003-09-29
DE69841340D1 (de) 2010-01-07

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