AU6229800A - Conditional homologous recombination of large genomic vector inserts - Google Patents
Conditional homologous recombination of large genomic vector insertsInfo
- Publication number
- AU6229800A AU6229800A AU62298/00A AU6229800A AU6229800A AU 6229800 A AU6229800 A AU 6229800A AU 62298/00 A AU62298/00 A AU 62298/00A AU 6229800 A AU6229800 A AU 6229800A AU 6229800 A AU6229800 A AU 6229800A
- Authority
- AU
- Australia
- Prior art keywords
- homologous recombination
- large genomic
- vector inserts
- genomic vector
- conditional
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/20—Pseudochromosomes, minichrosomosomes
- C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
- C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35698799A | 1999-07-20 | 1999-07-20 | |
US09356987 | 1999-07-20 | ||
USNOTGIVEN | 2000-06-23 | ||
PCT/US2000/019926 WO2001005962A1 (en) | 1999-07-20 | 2000-07-20 | Conditional homologous recombination of large genomic vector inserts |
Publications (1)
Publication Number | Publication Date |
---|---|
AU6229800A true AU6229800A (en) | 2001-02-05 |
Family
ID=23403819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU62298/00A Abandoned AU6229800A (en) | 1999-07-20 | 2000-07-20 | Conditional homologous recombination of large genomic vector inserts |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU6229800A (en) |
WO (1) | WO2001005962A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111471646A (en) * | 2020-04-20 | 2020-07-31 | 安徽中盛溯源生物科技有限公司 | Method for preparing iPSC by using free plasmid vector screened by non-antibiotics |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2527456B1 (en) | 2004-10-22 | 2018-05-16 | Revivicor Inc. | Transgenic porcines lacking endogenous immunoglobulin light chain |
EP2265730B1 (en) | 2008-03-12 | 2018-06-06 | The Rockefeller University | Methods and compositions for translational profiling and molecular phenotyping |
WO2010051288A1 (en) | 2008-10-27 | 2010-05-06 | Revivicor, Inc. | Immunocompromised ungulates |
EP2456894A4 (en) | 2009-07-23 | 2013-09-25 | Chromatin Inc | Sorghum centromere sequences and minichromosomes |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0485701A1 (en) * | 1990-09-28 | 1992-05-20 | American Cyanamid Company | Insertion of DNA by modified transposons |
US6069010A (en) * | 1995-09-11 | 2000-05-30 | Axys Pharmaceuticals, Inc. | High throughput gene inactivation with large scale gene targeting |
FR2738842B1 (en) * | 1995-09-15 | 1997-10-31 | Rhone Poulenc Rorer Sa | CIRCULAR DNA MOLECULE WITH ORIGIN OF CONDITIONAL REPLICATION, THEIR PREPARATION METHOD AND THEIR USE IN GENE THERAPY |
US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
JP2002515764A (en) * | 1997-06-23 | 2002-05-28 | ザ ロックフェラー ユニヴァーシティ | Method for preforming modifications based on homologous recombination of nucleic acids in non-recombinant cells and use of the modified nucleic acid products |
US6509156B1 (en) * | 1997-12-05 | 2003-01-21 | Europaisches Laboratorium Fur Molekularoiologie (Embl) | DNA cloning method relying on the E. coli recE/recT recombination system |
-
2000
- 2000-07-20 AU AU62298/00A patent/AU6229800A/en not_active Abandoned
- 2000-07-20 WO PCT/US2000/019926 patent/WO2001005962A1/en active Application Filing
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111471646A (en) * | 2020-04-20 | 2020-07-31 | 安徽中盛溯源生物科技有限公司 | Method for preparing iPSC by using free plasmid vector screened by non-antibiotics |
CN111471646B (en) * | 2020-04-20 | 2022-07-05 | 安徽中盛溯源生物科技有限公司 | Method for preparing iPSC by using free plasmid vector screened by non-antibiotics |
Also Published As
Publication number | Publication date |
---|---|
WO2001005962A1 (en) | 2001-01-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase |