DE69735382T2 - Kationische reagenzien zür transfektion - Google Patents
Kationische reagenzien zür transfektion Download PDFInfo
- Publication number
- DE69735382T2 DE69735382T2 DE69735382T DE69735382T DE69735382T2 DE 69735382 T2 DE69735382 T2 DE 69735382T2 DE 69735382 T DE69735382 T DE 69735382T DE 69735382 T DE69735382 T DE 69735382T DE 69735382 T2 DE69735382 T2 DE 69735382T2
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- Germany
- Prior art keywords
- alkyl
- straight
- chain
- branched
- alkynyl
- Prior art date
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- Expired - Lifetime
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
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- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
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- A—HUMAN NECESSITIES
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- A61K9/00—Medicinal preparations characterised by special physical form
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- A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers
- A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
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- Public Health (AREA)
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- Pharmacology & Pharmacy (AREA)
- Biophysics (AREA)
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- Wood Science & Technology (AREA)
- Biotechnology (AREA)
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- Zoology (AREA)
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- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3031596P | 1996-11-04 | 1996-11-04 | |
| US30315P | 1996-11-04 | ||
| PCT/EP1997/006035 WO1998019709A2 (en) | 1996-11-04 | 1997-11-03 | Cationic reagents for transfection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69735382D1 DE69735382D1 (de) | 2006-04-27 |
| DE69735382T2 true DE69735382T2 (de) | 2006-11-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69735382T Expired - Lifetime DE69735382T2 (de) | 1996-11-04 | 1997-11-03 | Kationische reagenzien zür transfektion |
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| Country | Link |
|---|---|
| US (2) | US6733777B2 (enExample) |
| EP (1) | EP1003556B1 (enExample) |
| JP (1) | JP4175667B2 (enExample) |
| AU (1) | AU738384B2 (enExample) |
| CA (1) | CA2270396C (enExample) |
| DE (1) | DE69735382T2 (enExample) |
| WO (1) | WO1998019709A2 (enExample) |
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|---|---|---|---|---|
| US5674908A (en) | 1993-12-20 | 1997-10-07 | Life Technologies, Inc. | Highly packed polycationic ammonium, sulfonium and phosphonium lipids |
| US6989434B1 (en) | 1994-02-11 | 2006-01-24 | Invitrogen Corporation | Reagents for intracellular delivery of macromolecules |
| CA2270396C (en) * | 1996-11-04 | 2008-03-11 | Qiagen Gmbh | Cationic reagents for transfection |
| JP4854853B2 (ja) * | 1998-11-12 | 2012-01-18 | ライフ テクノロジーズ コーポレーション | トランスフェクション薬剤 |
| US7683035B1 (en) | 1999-02-23 | 2010-03-23 | Qiagen, Gmbh | Method of stabilizing and/or isolating nucleic acids |
| US20030096414A1 (en) | 2001-03-27 | 2003-05-22 | Invitrogen Corporation | Culture medium for cell growth and transfection |
| US7906122B2 (en) | 2003-06-18 | 2011-03-15 | Yissum Research Development Company Of The Hebrew University Of Jersusalem | Sphingoid polyalkylamine conjugates for Hepatitis B virus vaccination |
| EP1638610B1 (en) | 2003-06-18 | 2015-03-18 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Sphingoid polyalkylamine conjugates for vaccination |
| US20060211004A1 (en) | 2005-02-15 | 2006-09-21 | Ilsley Diane D | Methods and compositions for determining non-specific cytotoxicity of a transfection agent |
| EP1871914A4 (en) * | 2005-04-01 | 2011-04-27 | Life Technologies Corp | METHOD AND COMPOSITIONS FOR INTRODUCING BIOPOLYMERS IN CELLS |
| US9303080B2 (en) | 2006-01-13 | 2016-04-05 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, National Institutes Of Health | Codon optimized IL-15 and IL-15R-alpha genes for expression in mammalian cells |
| CA2686735A1 (en) | 2007-05-04 | 2008-11-13 | Mdrna, Inc. | Amino acid lipids and uses thereof |
| JP2011521649A (ja) | 2008-05-30 | 2011-07-28 | イェール ユニバーシティー | 遺伝子発現を改変するための標的化オリゴヌクレオチド組成物 |
| EP2902013A1 (en) | 2008-10-16 | 2015-08-05 | Marina Biotech, Inc. | Processes and Compositions for Liposomal and Efficient Delivery of Gene Silencing Therapeutics |
| RS58405B1 (sr) | 2009-12-01 | 2019-04-30 | Translate Bio Inc | Stereoidni derivati za isporuku irnk u humanim genetskim oboljenjima |
| US20130052221A1 (en) | 2010-02-26 | 2013-02-28 | The Govt. of the U.S, as represented by The Sec. of The Dept. of Health and Human Services | Dna-protein vaccination protocols |
| WO2011120023A1 (en) | 2010-03-26 | 2011-09-29 | Marina Biotech, Inc. | Nucleic acid compounds for inhibiting survivin gene expression uses thereof |
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| US3966630A (en) * | 1972-03-24 | 1976-06-29 | Petrolite Corporation | Use of polyquaternary ammonium methylene phosphonates in chelating or scale inhibition |
| US4022833A (en) * | 1973-02-14 | 1977-05-10 | Sterling Drug Inc. | N,N'-bridged-bis[2-alkyl-2-hydroxyethylamines] |
| US5733572A (en) * | 1989-12-22 | 1998-03-31 | Imarx Pharmaceutical Corp. | Gas and gaseous precursor filled microspheres as topical and subcutaneous delivery vehicles |
| US5220454A (en) * | 1990-03-30 | 1993-06-15 | Nikon Corporation | Cata-dioptric reduction projection optical system |
| US5283185A (en) * | 1991-08-28 | 1994-02-01 | University Of Tennessee Research Corporation | Method for delivering nucleic acids into cells |
| DE19505368A1 (de) * | 1995-02-17 | 1996-08-22 | Huels Chemische Werke Ag | Amphiphile Verbindungen mit mindestens zwei hydrophilen und mindestens zwei hydrophoben Gruppen auf der Basis von Dicarbonsäurediamiden |
| WO1996034109A1 (en) * | 1995-04-25 | 1996-10-31 | Vical Incorporated | Single-vial formulations of dna/lipid complexes |
| US5744335A (en) * | 1995-09-19 | 1998-04-28 | Mirus Corporation | Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein |
| US5780448A (en) * | 1995-11-07 | 1998-07-14 | Ottawa Civic Hospital Loeb Research | DNA-based vaccination of fish |
| CA2270396C (en) * | 1996-11-04 | 2008-03-11 | Qiagen Gmbh | Cationic reagents for transfection |
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1997
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- 1997-11-03 AU AU53156/98A patent/AU738384B2/en not_active Expired
- 1997-11-03 DE DE69735382T patent/DE69735382T2/de not_active Expired - Lifetime
- 1997-11-03 JP JP52084398A patent/JP4175667B2/ja not_active Expired - Lifetime
- 1997-11-03 EP EP97950064A patent/EP1003556B1/en not_active Expired - Lifetime
- 1997-11-03 WO PCT/EP1997/006035 patent/WO1998019709A2/en not_active Ceased
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1999
- 1999-05-04 US US09/304,995 patent/US6733777B2/en not_active Expired - Lifetime
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2003
- 2003-11-25 US US10/721,532 patent/US20040077582A1/en not_active Abandoned
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| EP1003556B1 (en) | 2006-03-01 |
| AU738384B2 (en) | 2001-09-20 |
| JP4175667B2 (ja) | 2008-11-05 |
| US6733777B2 (en) | 2004-05-11 |
| DE69735382D1 (de) | 2006-04-27 |
| CA2270396C (en) | 2008-03-11 |
| WO1998019709A3 (en) | 1998-07-30 |
| CA2270396A1 (en) | 1998-05-14 |
| US20040077582A1 (en) | 2004-04-22 |
| WO1998019709A2 (en) | 1998-05-14 |
| US20010048939A1 (en) | 2001-12-06 |
| JP2001503751A (ja) | 2001-03-21 |
| EP1003556A2 (en) | 2000-05-31 |
| AU5315698A (en) | 1998-05-29 |
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