DE69725881D1 - Verfahren zur expression von heterologen proteinen in hefe - Google Patents

Verfahren zur expression von heterologen proteinen in hefe

Info

Publication number
DE69725881D1
DE69725881D1 DE69725881T DE69725881T DE69725881D1 DE 69725881 D1 DE69725881 D1 DE 69725881D1 DE 69725881 T DE69725881 T DE 69725881T DE 69725881 T DE69725881 T DE 69725881T DE 69725881 D1 DE69725881 D1 DE 69725881D1
Authority
DE
Germany
Prior art keywords
isoforms
proteins
pdgf
clipped
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69725881T
Other languages
English (en)
Other versions
DE69725881T2 (de
Inventor
Patricia Tekamp-Olson
P Merryweather
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of DE69725881D1 publication Critical patent/DE69725881D1/de
Publication of DE69725881T2 publication Critical patent/DE69725881T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1858—Platelet-derived growth factor [PDGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0066—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A61L2300/414—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Materials Engineering (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Rheumatology (AREA)
  • Dermatology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
DE69725881T 1996-12-13 1997-12-12 Verfahren zur expression von heterologen proteinen in hefe Expired - Lifetime DE69725881T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3272096P 1996-12-13 1996-12-13
US32720P 1996-12-13
PCT/US1997/022647 WO1998026080A1 (en) 1996-12-13 1997-12-12 Method for expression of heterologous proteins in yeast

Publications (2)

Publication Number Publication Date
DE69725881D1 true DE69725881D1 (de) 2003-12-11
DE69725881T2 DE69725881T2 (de) 2004-09-09

Family

ID=21866458

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69725881T Expired - Lifetime DE69725881T2 (de) 1996-12-13 1997-12-12 Verfahren zur expression von heterologen proteinen in hefe
DE69738768T Expired - Lifetime DE69738768D1 (de) 1996-12-13 1997-12-12 Analyse und separation von pdgf-proteinen
DE69739319T Expired - Lifetime DE69739319D1 (de) 1996-12-13 1997-12-12 Verfahren zur Expression von PDGF oder IGR Proteinen in Hefe

Family Applications After (2)

Application Number Title Priority Date Filing Date
DE69738768T Expired - Lifetime DE69738768D1 (de) 1996-12-13 1997-12-12 Analyse und separation von pdgf-proteinen
DE69739319T Expired - Lifetime DE69739319D1 (de) 1996-12-13 1997-12-12 Verfahren zur Expression von PDGF oder IGR Proteinen in Hefe

Country Status (9)

Country Link
US (10) US6083910A (de)
EP (4) EP1365028B1 (de)
JP (4) JP4341859B2 (de)
AT (3) ATE253120T1 (de)
AU (2) AU5465498A (de)
DE (3) DE69725881T2 (de)
ES (1) ES2306462T3 (de)
PT (1) PT948538E (de)
WO (2) WO1998025963A1 (de)

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US6897043B2 (en) 2005-05-24
ATE426030T1 (de) 2009-04-15
WO1998025963A1 (en) 1998-06-18
DE69738768D1 (de) 2008-07-24
EP1365028A1 (de) 2003-11-26
JP2008031173A (ja) 2008-02-14
US20060252695A1 (en) 2006-11-09
JP2008119008A (ja) 2008-05-29
EP1935901A1 (de) 2008-06-25
US6706496B2 (en) 2004-03-16
EP0948538B1 (de) 2008-06-11
EP0946736B1 (de) 2003-10-29
EP1365028B1 (de) 2009-03-18
US6312923B1 (en) 2001-11-06
EP0948538A1 (de) 1999-10-13
DE69725881T2 (de) 2004-09-09
PT948538E (pt) 2008-08-05
US7166446B2 (en) 2007-01-23
ATE398139T1 (de) 2008-07-15
US20030036511A1 (en) 2003-02-20
US6017731A (en) 2000-01-25
WO1998026080A1 (en) 1998-06-18
DE69739319D1 (de) 2009-04-30
ATE253120T1 (de) 2003-11-15
US20050124042A1 (en) 2005-06-09
US20020055169A1 (en) 2002-05-09
JP4341859B2 (ja) 2009-10-14
JP2001518073A (ja) 2001-10-09
US6083723A (en) 2000-07-04
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JP4275741B2 (ja) 2009-06-10
US6083910A (en) 2000-07-04
US7084262B2 (en) 2006-08-01
EP0946736A1 (de) 1999-10-06
US20030077831A1 (en) 2003-04-24
US6448382B1 (en) 2002-09-10
ES2306462T3 (es) 2008-11-01
AU5794598A (en) 1998-07-03
AU5465498A (en) 1998-07-03

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