DE69529337D1 - Luziferasen - Google Patents

Luziferasen

Info

Publication number
DE69529337D1
DE69529337D1 DE69529337T DE69529337T DE69529337D1 DE 69529337 D1 DE69529337 D1 DE 69529337D1 DE 69529337 T DE69529337 T DE 69529337T DE 69529337 T DE69529337 T DE 69529337T DE 69529337 D1 DE69529337 D1 DE 69529337D1
Authority
DE
Germany
Prior art keywords
luciferase
preparation
retains
room temperature
stored
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69529337T
Other languages
English (en)
Other versions
DE69529337T2 (de
Inventor
Robin Lowe
John White
Augustus Murray
James Squirrel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9405750A external-priority patent/GB9405750D0/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of DE69529337D1 publication Critical patent/DE69529337D1/de
Application granted granted Critical
Publication of DE69529337T2 publication Critical patent/DE69529337T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69529337T 1994-03-23 1995-03-22 Luziferasen Expired - Lifetime DE69529337T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9405750A GB9405750D0 (en) 1994-03-23 1994-03-23 Luciferases
GBGB9501170.6A GB9501170D0 (en) 1994-03-23 1995-01-20 Luciferases
PCT/GB1995/000629 WO1995025798A1 (en) 1994-03-23 1995-03-22 Luciferases

Publications (2)

Publication Number Publication Date
DE69529337D1 true DE69529337D1 (de) 2003-02-13
DE69529337T2 DE69529337T2 (de) 2003-10-16

Family

ID=26304561

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69529337T Expired - Lifetime DE69529337T2 (de) 1994-03-23 1995-03-22 Luziferasen

Country Status (17)

Country Link
US (1) US6132983A (de)
EP (2) EP0751996B1 (de)
JP (3) JP4490508B2 (de)
KR (1) KR100392020B1 (de)
CN (1) CN100387720C (de)
AT (1) ATE230800T1 (de)
AU (1) AU697883B2 (de)
BR (1) BR9507140A (de)
CA (1) CA2186144C (de)
DE (1) DE69529337T2 (de)
DK (1) DK0751996T3 (de)
ES (1) ES2185697T3 (de)
FI (1) FI120096B (de)
GB (1) GB9501170D0 (de)
NO (1) NO319706B1 (de)
RU (1) RU2192467C2 (de)
WO (1) WO1995025798A1 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases
GB2311525B (en) * 1995-01-20 1998-11-11 Secr Defence Mutant Luciferases
JP4342608B2 (ja) * 1995-01-20 2009-10-14 スリーエム イノベイティブ プロパティーズ カンパニー 突然変異体ルシフェラーゼ
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
EP1015601B1 (de) * 1997-09-19 2015-01-07 Promega Corporation Thermostabile luciferasen und verfahren zu deren herstellung
GB9722481D0 (en) * 1997-10-25 1997-12-24 Secr Defence Expression system
US6117643A (en) * 1997-11-25 2000-09-12 Ut Battelle, Llc Bioluminescent bioreporter integrated circuit
DE69938293T2 (de) * 1998-03-27 2009-03-12 Bruce J. Beverly Hills Bryan Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
JP5154724B2 (ja) * 1999-10-26 2013-02-27 プロメガ コーポレイション 変異型ルシフェラーゼ
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US20020073441A1 (en) 2000-12-13 2002-06-13 Ross Brian D. Compositions and methods for detecting proteolytic activity
GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
CN101098958A (zh) 2002-02-21 2008-01-02 免疫医疗疫苗公司 间质肺病毒株及其在疫苗制剂中以及用作抗原性序列表达载体的用途
US20040142356A1 (en) * 2002-10-30 2004-07-22 Stacey Patterson Modified luciferase nucleic acids and methods of use
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
JP2006521116A (ja) 2003-03-27 2006-09-21 ピーティーシー セラピューティクス，インコーポレーテッド 抗真菌性および／または抗増殖性分子を同定するためのｔＲＮＡスプライシング経路の酵素の標的化
EP2319318A3 (de) 2003-03-27 2011-08-17 PTC Therapeutics, Inc. Verfahren zur Identifizierung von Verbindungen mit tRNA-Splicing Endonuklease als Aufhänger, und Verwendungen für diese Verbindungen als anti-Proliferationsmittel
AU2004236536A1 (en) * 2003-05-06 2004-11-18 National Institute Of Advanced Industrial Science And Technology Multiple gene transcription activity determining sysyem
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
US7807429B2 (en) 2006-02-13 2010-10-05 Connecticut College Isolated luciferase gene of L. italica
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
US8183007B2 (en) * 2008-07-22 2012-05-22 Promega Corporation ADP detection based methods using adenylate cyclase and bioluminescence
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ
JP2011120559A (ja) * 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
US20110076706A1 (en) * 2009-06-26 2011-03-31 Genprime, Inc. Methods and kits for the rapid detection of microorganisms
PL2635583T3 (pl) 2010-11-02 2015-11-30 Promega Corp Pochodne koelenterazyny i metody ich wykorzystania
CA2817102C (en) 2010-11-02 2020-07-28 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
CN105121653B (zh) 2013-02-22 2020-01-24 普洛麦格公司 用于荧光素酶和核苷磷酸的发光检测的稳定制剂
EP3099691B1 (de) 2014-01-29 2019-11-20 Promega Corporation Pro-substrate für lebendzellanwendungen
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
WO2016057084A1 (en) 2014-10-08 2016-04-14 Promega Corporation Bioluminescent succinate detection assay
CN105200022B (zh) * 2015-11-03 2018-11-16 南京工业大学 萤火虫荧光素酶热稳定性改造的方法
JP6349003B1 (ja) * 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
JPWO2021162123A1 (de) 2020-02-14 2021-08-19
CN114015664B (zh) * 2021-12-06 2023-07-21 郑州伊美诺生物技术有限公司 荧光素酶突变体及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5229285A (en) * 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
JP3048466B2 (ja) * 1991-06-27 2000-06-05 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
DE4124495A1 (de) * 1991-07-24 1993-01-28 Happich Gmbh Gebr Fenstereinfassrahmen, insbesondere fuer fahrzeuge
GB9501170D0 (en) * 1994-03-23 1995-03-08 Secr Defence Luciferases

Also Published As

Publication number Publication date
EP1281762A3 (de) 2003-11-05
FI963741A (fi) 1996-11-20
KR970702369A (ko) 1997-05-13
NO319706B1 (no) 2005-09-05
FI120096B (fi) 2009-06-30
GB9501170D0 (en) 1995-03-08
AU697883B2 (en) 1998-10-22
CN1149318A (zh) 1997-05-07
JP2006271393A (ja) 2006-10-12
BR9507140A (pt) 1997-09-30
WO1995025798A1 (en) 1995-09-28
ES2185697T3 (es) 2003-05-01
JPH09510610A (ja) 1997-10-28
KR100392020B1 (ko) 2003-10-22
DE69529337T2 (de) 2003-10-16
JP4490508B2 (ja) 2010-06-30
NO963983L (no) 1996-09-23
CA2186144A1 (en) 1995-09-28
CN100387720C (zh) 2008-05-14
AU1954595A (en) 1995-10-09
NO963983D0 (no) 1996-09-23
EP0751996A1 (de) 1997-01-08
JP2010088440A (ja) 2010-04-22
CA2186144C (en) 2009-12-22
DK0751996T3 (da) 2003-02-24
EP0751996B1 (de) 2003-01-08
EP1281762B1 (de) 2014-12-31
FI963741A0 (fi) 1996-09-20
US6132983A (en) 2000-10-17
ATE230800T1 (de) 2003-01-15
RU2192467C2 (ru) 2002-11-10
EP1281762A2 (de) 2003-02-05

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: 3M INNOVATIVE PROPERTIES CO., SAINT PAUL, MINN, US

8327 Change in the person/name/address of the patent owner

Owner name: PROMEGA CORP., MADISON, WIS., US