DE69524560D1 - Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure - Google Patents

Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure

Info

Publication number
DE69524560D1
DE69524560D1 DE69524560T DE69524560T DE69524560D1 DE 69524560 D1 DE69524560 D1 DE 69524560D1 DE 69524560 T DE69524560 T DE 69524560T DE 69524560 T DE69524560 T DE 69524560T DE 69524560 D1 DE69524560 D1 DE 69524560D1
Authority
DE
Germany
Prior art keywords
nucleic acids
capturing
nucleic acid
selectively releasing
releasing nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69524560T
Other languages
English (en)
Other versions
DE69524560T2 (de
Inventor
Tobias E Ekeze
John Wesley Backus
David John Sharkey
Richard Calvin Sutton
Joanne Hansen Kerschner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69524560D1 publication Critical patent/DE69524560D1/de
Application granted granted Critical
Publication of DE69524560T2 publication Critical patent/DE69524560T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69524560T 1994-09-13 1995-09-12 Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure Expired - Lifetime DE69524560T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/305,769 US5622822A (en) 1994-09-13 1994-09-13 Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same

Publications (2)

Publication Number Publication Date
DE69524560D1 true DE69524560D1 (de) 2002-01-24
DE69524560T2 DE69524560T2 (de) 2002-05-29

Family

ID=23182269

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69524560T Expired - Lifetime DE69524560T2 (de) 1994-09-13 1995-09-12 Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure

Country Status (6)

Country Link
US (1) US5622822A (de)
EP (1) EP0726312B1 (de)
JP (1) JP3727692B2 (de)
AT (1) ATE210725T1 (de)
CA (1) CA2157968C (de)
DE (1) DE69524560T2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846968B1 (en) 1988-02-26 2005-01-25 Large Scale Biology Corporation Production of lysosomal enzymes in plants by transient expression
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69805649T2 (de) * 1997-12-06 2002-12-05 Dna Res Innovations Ltd Isolierung von nukleinsäuren
AU2862100A (en) * 1999-01-27 2000-08-18 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
US7683035B1 (en) 1999-02-23 2010-03-23 Qiagen, Gmbh Method of stabilizing and/or isolating nucleic acids
ES2228520T3 (es) * 1999-05-04 2005-04-16 Ortho-Clinical Diagnostics, Inc. Capatura rapida y eficaz de adn de una muestra sin usar reactivo de lisis celular.
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid
US6406851B1 (en) * 2000-01-28 2002-06-18 Agilent Technologies, Inc. Method for coating a substrate quickly and uniformly with a small volume of fluid
US6730306B1 (en) 2000-03-08 2004-05-04 Large Scale Biology Corporation Parvovirus vaccine as viral coat protein fusions
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
DE60119533T2 (de) 2000-05-01 2007-05-16 Eiken Kagaku K.K. Verfahren zur erkennung des produkts einer nukleinsäuresynthetisierungsreaktion
US6806051B2 (en) * 2000-09-25 2004-10-19 Picoliter Inc. Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy
US20040106109A1 (en) * 2001-10-02 2004-06-03 Belly Robert T Detection of ras mutations
US6919182B2 (en) * 2001-10-09 2005-07-19 Sysmex Corporation Method of detecting gene as amplified product by gene amplification and reagent kit therefor
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
JP4437218B2 (ja) * 2002-11-26 2010-03-24 日生バイオ株式会社 Ｒｎａ−プロタミン複合体、その製造方法およびそれを含む健康食品
US9566558B2 (en) * 2004-09-09 2017-02-14 Institut Curie Device for manipulation of packets in micro-containers, in particular in microchannels
US20090208919A1 (en) * 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
US8288169B2 (en) * 2005-01-21 2012-10-16 Argylla Technologies Surface mediated self-assembly of nanoparticles
US7964380B2 (en) * 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
US20100159528A1 (en) * 2007-06-13 2010-06-24 Ge Healthcare Bio-Science Corp. Polymerase stabilization
WO2011081869A2 (en) * 2009-12-14 2011-07-07 Betty Wu Method and materials for separating nucleic acid materials
EP2576840B1 (de) 2010-05-25 2018-10-17 QIAGEN Gaithersburg, Inc. Hybrid-capture-assay mit schneller ergebnislieferung und zugehörige strategisch gekürzte sonden
ES2693672T3 (es) 2011-09-26 2018-12-13 Qiagen Gmbh Método rápido para aislar ácidos nucleicos extracelulares
PT2854981T (pt) * 2012-05-24 2018-10-31 Meridian Bioscience Inc Método de fracionamento e deteção de ácido nucleico
US9822404B2 (en) 2012-11-07 2017-11-21 Qiagen Gmbh Control for diagnostic assay
EP3885438A1 (de) 2015-06-10 2021-09-29 QIAGEN GmbH Verfahren zur isolierung extrazellulärer nukleinsäuren mittels anionenaustauschpartikeln
DE102017200332A1 (de) * 2017-01-11 2018-07-12 Robert Bosch Gmbh Verfahren zum Extrahieren von DNS

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3176006A (en) * 1963-08-06 1965-03-30 Zahn Rudolf Karl Process for treating desoxyribo-nucleic acid
DE2001902C3 (de) * 1970-01-16 1978-10-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen
IE38174B1 (en) * 1972-07-07 1978-01-18 Snam Progetti Process for purifying an enzyme
US4089747A (en) 1976-08-09 1978-05-16 Eastman Kodak Company Compositions for the detection of hydrogen peroxide
CA1180647A (en) 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4713326A (en) 1983-07-05 1987-12-15 Molecular Diagnostics, Inc. Coupling of nucleic acids to solid support by photochemical methods
DE3401620A1 (de) * 1984-01-18 1985-07-18 Boehringer Mannheim Gmbh, 6800 Mannheim Restriktionsendonuklease mae iii, ihre gewinnung und verwendung
US4920061A (en) 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4997772A (en) 1987-09-18 1991-03-05 Eastman Kodak Company Water-insoluble particle and immunoreactive reagent, analytical elements and methods of use
JPH0736015B2 (ja) 1987-09-18 1995-04-19 イーストマン コダック カンパニー 診断試験キットおよびリガンドの測定方法
US4914210A (en) 1987-10-02 1990-04-03 Cetus Corporation Oligonucleotide functionalizing reagents
US4962029A (en) 1987-10-02 1990-10-09 Cetus Corporation Covalent oligonucleotide-horseradish peroxidase conjugate
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
IL88923A (en) 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
EP0356395A3 (de) * 1988-08-26 1991-09-04 Ciba-Geigy Ag Flankierende Peptide und Verfahren zu ihrer Herstellung
US5229297A (en) 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
CA2026573A1 (en) 1989-02-03 1990-08-04 John B. Findlay Nucleic acid test article and its use to detect a predetermined nucleic acid
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5092992A (en) * 1989-06-07 1992-03-03 J. T. Baker Inc. Polyethyleneimine matrixes for affinity chromatography
US5141857A (en) * 1989-06-09 1992-08-25 Gene-Trak Systems Purification of q beta replicase
US5089233A (en) 1989-06-12 1992-02-18 Eastman Kodak Company Processing apparatus for a chemical reaction pack
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
DE69131891T2 (de) 1990-02-16 2000-06-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5155166A (en) 1990-06-18 1992-10-13 Eastman Kodak Company Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5173260A (en) 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
EP0487218B1 (de) 1990-10-31 1997-12-29 Tosoh Corporation Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
CA2083173A1 (en) 1991-03-21 1992-09-22 Leonard J. Seaberg Element and method for nucleic acid amplification and detection using adhered probes
CA2063855C (en) * 1991-04-03 1997-08-26 Will Bloch Precision and accuracy of anion-exchange separation of nucleic acids
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5473060A (en) * 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications

Also Published As

Publication number Publication date
CA2157968A1 (en) 1996-03-14
EP0726312A2 (de) 1996-08-14
ATE210725T1 (de) 2001-12-15
JP3727692B2 (ja) 2005-12-14
JPH08173159A (ja) 1996-07-09
CA2157968C (en) 2009-11-10
EP0726312A3 (de) 1998-01-14
DE69524560T2 (de) 2002-05-29
EP0726312B1 (de) 2001-12-12
US5622822A (en) 1997-04-22

Similar Documents

Publication Publication Date Title
DE69524560D1 (de) Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure
DK0707077T3 (da) Fremgangsmåder til indfangning og selektiv frigörelse af nucleinsyrer under anvendelse af svagt basisk polymer og amplifikation af samme
ATE178098T1 (de) Vorrichtung zur dürchführung von magnetischen zyklusreactionen
ATE114334T1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
ATE282716T1 (de) Verfahren zur amplifikation von nukleinsäuresequenzen
DE68926991D1 (de) Verfahren zum Nachweis und zur Identifizierung von Nukleinsäure enthaltenden Teilen
DE69708865T2 (de) Verfahren zur direkten sequenzierung während "template"-amplifikation
ATE91708T1 (de) Verfahren zur freisetzung von rns und dns aus zellen.
DE50015331D1 (de) Verfahren zum anbinden von nukleinsäuren an eine festphase
ATE548464T1 (de) Detektion von nukleinsäuren durch typenspezifische hybrid-capture methode
CA2164706A1 (en) Magnetic cycle reaction
DE69031957D1 (de) Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut
DE69329636T2 (de) Verfahren zur Vervielfältigung von Nukleinsäuresequenzen
ATE156830T1 (de) Verfahren zur reinigung von nukleinsäuren
AT347877B (de) Verfahren zum entfernen von im wasser geloesten verunreinigungen und vorrichtung zur durchfuehrung des verfahrens
DE59000762D1 (de) Verfahren zur schnellen detektion von mikroorganismen in proben und vorrichtung zur durchfuehrung des verfahrens.
DK1206571T3 (da) Hurtig og effektiv indfangning af DNA fra pröve uden anvendelse af cellelyseringsreagens
ATA64687A (de) Vorrichtung und verfahren insbesondere zum erhoehen des kohlenstoffgehaltes von direkt reduziertem eisen
ATA95299A (de) Verfahren zur herstellung von ig g präparaten
ATE123315T1 (de) Verfahren zur reinigung, vervielfachung und zum nachweis einer nukleinsäure.
DE3770252D1 (de) Verfahren zur herstellung von eisen(iii)oxid und ammoniumsalzen und vorrichtung zur durchfuehrung des verfahrens.
DE60238199D1 (de) Automatisches verfahren zum nachweis pathogener organismen in wasser

Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: BOEHMERT & BOEHMERT, 28209 BREMEN