DE69233178D1 - Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt - Google Patents

Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt

Info

Publication number
DE69233178D1
DE69233178D1 DE69233178T DE69233178T DE69233178D1 DE 69233178 D1 DE69233178 D1 DE 69233178D1 DE 69233178 T DE69233178 T DE 69233178T DE 69233178 T DE69233178 T DE 69233178T DE 69233178 D1 DE69233178 D1 DE 69233178D1
Authority
DE
Germany
Prior art keywords
promotor
bacillus
variant
derived
alpha amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69233178T
Other languages
English (en)
Other versions
DE69233178T2 (de
Inventor
Troels Jorgensen
Borge Krag Diderichsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69233178D1 publication Critical patent/DE69233178D1/de
Publication of DE69233178T2 publication Critical patent/DE69233178T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69233178T 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt Expired - Lifetime DE69233178T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100343 1991-11-14
WOPCT/DK91/00343 1991-11-14
PCT/DK1992/000338 WO1993010249A1 (en) 1991-11-14 1992-11-13 A bacillus promoter derived from a variant of a bacillus licheniformis x-amylase promoter

Publications (2)

Publication Number Publication Date
DE69233178D1 true DE69233178D1 (de) 2003-10-02
DE69233178T2 DE69233178T2 (de) 2004-06-17

Family

ID=8153698

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69233178T Expired - Lifetime DE69233178T2 (de) 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt

Country Status (11)

Country Link
US (1) US5698415A (de)
EP (1) EP0667910B1 (de)
JP (1) JP3529774B2 (de)
AT (1) ATE248223T1 (de)
AU (1) AU661070B2 (de)
CA (1) CA2123496C (de)
DE (1) DE69233178T2 (de)
DK (1) DK0667910T3 (de)
FI (1) FI112668B (de)
IL (1) IL103750A0 (de)
WO (1) WO1993010249A1 (de)

Families Citing this family (38)

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US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
DK1995319T3 (da) 2002-04-10 2011-12-05 Novozymes As Forbedret bacillusværtscelle
US20050176097A1 (en) 2002-04-10 2005-08-11 Andersen Jens T. Bacillus host cell
EP1625208A4 (de) 2003-05-12 2006-10-18 Genencor Int Neues lipolytisches enzym lip2
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
DK1639107T3 (da) 2003-06-19 2013-11-18 Novozymes As Forbedrede proteaser og fremgangsmåder til fremstilling deraf
US20050221446A1 (en) * 2004-03-31 2005-10-06 Novozymes Biopolymer A/S. Methods for producing hyaluronic acid in a Bacillus cell
CN101006174B (zh) 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
EP2104739B1 (de) 2006-12-21 2013-06-19 Novozymes Inc. MODIFIZIERTE mRNA-STABILISIERENDE SEQUENZEN ZUR GENEXPRESSION IN BAKTERIENZELLEN
KR20100014500A (ko) 2007-03-23 2010-02-10 다니스코 유에스 인크. 성숙 아밀라아제 단백질에 ｎ-말단 첨가에 의한 아밀라아제 생성 향상
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
NZ587540A (en) 2008-03-28 2012-06-29 Danisco Us Inc Method for amplifying locus in bacterial cell
EP2420571A4 (de) 2009-01-16 2012-07-04 Otsuka Chemical Co Ltd Hochexprimierender promoter und verfahren zur herstellung eines genprodukts damit
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011000924A1 (en) 2009-07-03 2011-01-06 Abbott Products Gmbh Spray-dried amylase, pharmaceutical preparations comprising the same and use
EP2516636A1 (de) 2009-12-21 2012-10-31 Novozymes Inc. Verfahren zur herstellung von heterologen polypeptiden bei mutierenden bakterienzellen mit thiol-disulfid-oxidoreduktasemangel
WO2012127001A1 (en) 2011-03-23 2012-09-27 Novozymes A/S Methods for producing secreted polypeptides
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
EP3707252A1 (de) 2017-11-10 2020-09-16 Novozymes A/S Temperaturempfindliches cas9-protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
EP3927809A1 (de) 2019-02-20 2021-12-29 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und spurenelementezufuhr
EP3927837A1 (de) 2019-02-20 2021-12-29 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und einer magnesiumzufuhr
EP3969576A1 (de) 2019-05-15 2022-03-23 Novozymes A/S Temperaturempfindliche rna-geführte endonuklease
EP3990629A1 (de) 2019-06-25 2022-05-04 Novozymes A/S Gegenauswahl durch hemmung von bedingt essenziellen genen
KR20230042088A (ko) 2020-07-24 2023-03-27 바스프 에스이 알라닌 라세마아제 단일 결실 및 트랜스 보완
WO2022023370A1 (en) 2020-07-28 2022-02-03 Basf Se Industrial fermentation process for bacillus using temperature shift
MX2023001273A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion.
EP4189104A1 (de) 2020-07-28 2023-06-07 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung von partieller ernte
MX2023003276A (es) 2020-09-22 2023-05-03 Basf Se Método para recuperar una proteína de un caldo de fermentación que comprende un alto grado de células lisadas.
EP4225779A2 (de) 2020-10-07 2023-08-16 Basf Se Bacilluszelle mit reduzierter lipase- und/oder esteraseseitenaktivität
US20240052000A1 (en) 2020-12-15 2024-02-15 Novozymes A/S Mutated host cells with reduced cell motility on
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
WO2022269082A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus production host
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus
WO2024028338A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769327A (en) * 1985-03-29 1988-09-06 Biotechnica International, Inc. Secretion vector

Also Published As

Publication number Publication date
JP3529774B2 (ja) 2004-05-24
IL103750A0 (en) 1993-04-04
AU661070B2 (en) 1995-07-13
DK0667910T3 (da) 2003-12-01
US5698415A (en) 1997-12-16
WO1993010249A1 (en) 1993-05-27
AU2943092A (en) 1993-06-15
DE69233178T2 (de) 2004-06-17
FI112668B (fi) 2003-12-31
EP0667910A1 (de) 1995-08-23
EP0667910B1 (de) 2003-08-27
CA2123496A1 (en) 1993-05-27
FI942228A0 (fi) 1994-05-13
JPH07504085A (ja) 1995-05-11
CA2123496C (en) 2004-02-03
FI942228A (fi) 1994-07-13
ATE248223T1 (de) 2003-09-15

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