DE69233178D1 - Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt - Google Patents

Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt

Info

Publication number
DE69233178D1
DE69233178D1 DE69233178T DE69233178T DE69233178D1 DE 69233178 D1 DE69233178 D1 DE 69233178D1 DE 69233178 T DE69233178 T DE 69233178T DE 69233178 T DE69233178 T DE 69233178T DE 69233178 D1 DE69233178 D1 DE 69233178D1
Authority
DE
Germany
Prior art keywords
promotor
bacillus
variant
derived
alpha amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69233178T
Other languages
English (en)
Other versions
DE69233178T2 (de
Inventor
Troels Jorgensen
Borge Krag Diderichsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69233178D1 publication Critical patent/DE69233178D1/de
Publication of DE69233178T2 publication Critical patent/DE69233178T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
DE69233178T 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt Expired - Lifetime DE69233178T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
WOPCT/DK91/00343 1991-11-13
DK9100343 1991-11-14
PCT/DK1992/000338 WO1993010249A1 (en) 1991-11-14 1992-11-13 A bacillus promoter derived from a variant of a bacillus licheniformis x-amylase promoter

Publications (2)

Publication Number Publication Date
DE69233178D1 true DE69233178D1 (de) 2003-10-02
DE69233178T2 DE69233178T2 (de) 2004-06-17

Family

ID=8153698

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69233178T Expired - Lifetime DE69233178T2 (de) 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase-promotor einer variante des bacillus licheniformis abstammt

Country Status (11)

Country Link
US (1) US5698415A (de)
EP (1) EP0667910B1 (de)
JP (1) JP3529774B2 (de)
AT (1) ATE248223T1 (de)
AU (1) AU661070B2 (de)
CA (1) CA2123496C (de)
DE (1) DE69233178T2 (de)
DK (1) DK0667910T3 (de)
FI (1) FI112668B (de)
IL (1) IL103750A0 (de)
WO (1) WO1993010249A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
AU2003214034A1 (en) 2002-04-10 2003-11-17 Novozymes A/S Bacillus licheniformis mutant host cell
AU2003214036A1 (en) 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
WO2004101759A2 (en) 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip2
EP1625202A4 (de) 2003-05-12 2010-10-20 Genencor Int Neues lipolytisches enzym lip1
US20060236414A1 (en) 2003-06-19 2006-10-19 Novozymes A/S Proteases and methods for producing them
CA2563173A1 (en) * 2004-03-31 2005-10-20 Novozymes Biopolymer A/S Methods for producing hyaluronic acid in a bacillus cell
WO2005123915A1 (en) 2004-06-21 2005-12-29 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
DK2104739T3 (da) 2006-12-21 2013-10-07 Novozymes Inc Modificerede messenger-RNA-stabiliseringssekvenser til ekspression af gener i bakterieceller
EP2132316B1 (de) 2007-03-23 2015-03-18 Danisco US Inc. Verbesserte amylaseproduktion durch n-terminale addition an reifes amylase-protein
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
EP2257629B1 (de) 2008-03-28 2016-03-16 Danisco US Inc. Verfahren zur locusamplifizierung bei bakterienzellen
WO2010082619A1 (ja) 2009-01-16 2010-07-22 大塚化学株式会社 高発現プロモーターおよびこれを用いた遺伝子産物製造法
US20110306139A1 (en) 2009-02-27 2011-12-15 Novozymes A/S Mutant Cells Suitable For Recombinant Polypeptide Production
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011000924A1 (en) 2009-07-03 2011-01-06 Abbott Products Gmbh Spray-dried amylase, pharmaceutical preparations comprising the same and use
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
EP2689015B1 (de) 2011-03-23 2015-01-07 Novozymes A/S Verfahren zur herstellung sezernierter polypeptide
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
CN111511908A (zh) 2017-11-10 2020-08-07 诺维信公司 温度敏感性cas9蛋白
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
CN114127256A (zh) 2019-02-20 2022-03-01 巴斯夫欧洲公司 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺
EP3927837A1 (de) 2019-02-20 2021-12-29 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und einer magnesiumzufuhr
US20220275354A1 (en) 2019-05-15 2022-09-01 Novozymes A/S TEMPERATURE-SENSITIVE RNA- Guided Endonuclease
CN114207125A (zh) 2019-06-25 2022-03-18 诺维信公司 通过抑制条件性必需基因进行反向选择
WO2022018268A1 (en) 2020-07-24 2022-01-27 Basf Se Alanine racemase double deletion and transcomplementation
MX2023001273A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion.
KR20230042368A (ko) 2020-07-28 2023-03-28 바스프 에스이 온도 이동을 이용한 바실러스의 산업적 발효 방법
CA3186931A1 (en) 2020-07-28 2022-02-03 Andreas Daub Industrial fermentation process for bacillus using partial harvest
EP4217366A1 (de) 2020-09-22 2023-08-02 Basf Se Verfahren zur rückgewinnung eines proteins aus einer fermentationsbrühe mit hohem anteil an lysierten zellen
US20240060110A1 (en) 2020-10-07 2024-02-22 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
EP4263822A1 (de) 2020-12-15 2023-10-25 Novozymes A/S Mutierte wirtszellen mit reduzierter zellmotilität
EP4359546A1 (de) 2021-06-24 2024-05-01 Basf Se Verbesserte bacillus-wirtszelle mit verändertem rema/remb-protein
US20240318188A1 (en) 2021-06-24 2024-09-26 Basf Se Bacillus licheniformis host cell for production of a compound of interest with increased purity
US20240317820A1 (en) 2021-06-24 2024-09-26 Basf Se Improved bacillus production host
EP4453218A1 (de) 2021-12-20 2024-10-30 Basf Se Verfahren zur verbesserten herstellung intrazellulärer proteine in bacillus
WO2024028338A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells
WO2024146919A1 (en) 2023-01-05 2024-07-11 Basf Se Use of foldases to improve heterologous expression of secreted molecules

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769327A (en) * 1985-03-29 1988-09-06 Biotechnica International, Inc. Secretion vector

Also Published As

Publication number Publication date
EP0667910A1 (de) 1995-08-23
CA2123496A1 (en) 1993-05-27
AU661070B2 (en) 1995-07-13
AU2943092A (en) 1993-06-15
WO1993010249A1 (en) 1993-05-27
EP0667910B1 (de) 2003-08-27
CA2123496C (en) 2004-02-03
DE69233178T2 (de) 2004-06-17
FI112668B (fi) 2003-12-31
IL103750A0 (en) 1993-04-04
DK0667910T3 (da) 2003-12-01
JPH07504085A (ja) 1995-05-11
JP3529774B2 (ja) 2004-05-24
ATE248223T1 (de) 2003-09-15
FI942228A0 (fi) 1994-05-13
US5698415A (en) 1997-12-16
FI942228A (fi) 1994-07-13

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