ATE248223T1 - Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt - Google Patents

Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt

Info

Publication number
ATE248223T1
ATE248223T1 AT92923722T AT92923722T ATE248223T1 AT E248223 T1 ATE248223 T1 AT E248223T1 AT 92923722 T AT92923722 T AT 92923722T AT 92923722 T AT92923722 T AT 92923722T AT E248223 T1 ATE248223 T1 AT E248223T1
Authority
AT
Austria
Prior art keywords
bacillus
promoter
variant
alpha
pct
Prior art date
Application number
AT92923722T
Other languages
English (en)
Inventor
Steen Troels Jorgensen
Borge Krag Diderichsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE248223T1 publication Critical patent/ATE248223T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT92923722T 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt ATE248223T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK9100343 1991-11-14
PCT/DK1992/000338 WO1993010249A1 (en) 1991-11-14 1992-11-13 A bacillus promoter derived from a variant of a bacillus licheniformis x-amylase promoter

Publications (1)

Publication Number Publication Date
ATE248223T1 true ATE248223T1 (de) 2003-09-15

Family

ID=8153698

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92923722T ATE248223T1 (de) 1991-11-14 1992-11-13 Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt

Country Status (11)

Country Link
US (1) US5698415A (de)
EP (1) EP0667910B1 (de)
JP (1) JP3529774B2 (de)
AT (1) ATE248223T1 (de)
AU (1) AU661070B2 (de)
CA (1) CA2123496C (de)
DE (1) DE69233178T2 (de)
DK (1) DK0667910T3 (de)
FI (1) FI112668B (de)
IL (1) IL103750A0 (de)
WO (1) WO1993010249A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
US7521204B2 (en) 2002-04-10 2009-04-21 Novozymes A/S Bacillus host cell
CN1659282A (zh) 2002-04-10 2005-08-24 诺维信公司 地衣芽孢杆菌的突变宿主细胞
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
US20070213518A1 (en) 2003-05-12 2007-09-13 Jones Brian E Novel Lipolytic Enzyme Lip2
EP1639107B1 (de) 2003-06-19 2013-08-14 Novozymes A/S Verbesserte proteasen und methoden zu deren herstellung
JP2007535917A (ja) * 2004-03-31 2007-12-13 ノボザイムス バイオポリマー アクティーゼルスカブ 細菌細胞中でのヒアルロン酸の生産方法
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
EP2104739B1 (de) 2006-12-21 2013-06-19 Novozymes Inc. MODIFIZIERTE mRNA-STABILISIERENDE SEQUENZEN ZUR GENEXPRESSION IN BAKTERIENZELLEN
US8809031B2 (en) 2007-03-23 2014-08-19 Danisco Us Inc. Enhanced amylase production by N-terminal addition to mature amylase protein
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
US9528114B2 (en) 2008-03-28 2016-12-27 Danisco Us Inc. Method for amplifying locus in bacterial cell
JPWO2010082619A1 (ja) 2009-01-16 2012-07-05 大塚化学株式会社 高発現プロモーターおよびこれを用いた遺伝子産物製造法
US20110306139A1 (en) 2009-02-27 2011-12-15 Novozymes A/S Mutant Cells Suitable For Recombinant Polypeptide Production
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011000924A1 (en) 2009-07-03 2011-01-06 Abbott Products Gmbh Spray-dried amylase, pharmaceutical preparations comprising the same and use
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
CN103443278B (zh) 2011-03-23 2017-07-04 诺维信公司 生产分泌型多肽的方法
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
EP3969576A1 (de) 2019-05-15 2022-03-23 Novozymes A/S Temperaturempfindliche rna-geführte endonuklease
EP3990629A1 (de) 2019-06-25 2022-05-04 Novozymes A/S Gegenauswahl durch hemmung von bedingt essenziellen genen
CA3186383A1 (en) 2020-07-24 2022-01-27 Basf Se Alanine racemase single deletion and transcomplementation
US20230272333A1 (en) 2020-07-28 2023-08-31 Basf Se Industrial fermentation process for bacillus using partial harvest
EP4189062A1 (de) 2020-07-28 2023-06-07 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung von vorschubratenverschiebung
MX2023001272A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de temperatura.
EP4217366A1 (de) 2020-09-22 2023-08-02 Basf Se Verfahren zur rückgewinnung eines proteins aus einer fermentationsbrühe mit hohem anteil an lysierten zellen
US20240060110A1 (en) 2020-10-07 2024-02-22 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
CN116710471A (zh) 2020-12-15 2023-09-05 诺维信公司 具有减少的细胞运动的突变的宿主细胞
CN117813316A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 用于生产纯度更高的感兴趣的化合物的地衣芽孢杆菌宿主细胞
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
CN117813317A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 改良的芽孢杆菌生产宿主
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus
WO2024028338A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986005812A1 (en) * 1985-03-29 1986-10-09 Biotechnica International, Inc. Secretion vector

Also Published As

Publication number Publication date
EP0667910B1 (de) 2003-08-27
JP3529774B2 (ja) 2004-05-24
FI942228A (fi) 1994-07-13
DE69233178D1 (de) 2003-10-02
FI942228A0 (fi) 1994-05-13
FI112668B (fi) 2003-12-31
WO1993010249A1 (en) 1993-05-27
AU2943092A (en) 1993-06-15
EP0667910A1 (de) 1995-08-23
CA2123496C (en) 2004-02-03
US5698415A (en) 1997-12-16
CA2123496A1 (en) 1993-05-27
DE69233178T2 (de) 2004-06-17
JPH07504085A (ja) 1995-05-11
AU661070B2 (en) 1995-07-13
IL103750A0 (en) 1993-04-04
DK0667910T3 (da) 2003-12-01

Similar Documents

Publication Publication Date Title
ATE248223T1 (de) Ein bacillus-promotor, der von dem alpha-amylase- promotor einer variante des bacillus licheniformis abstammt
DK351387A (da) Blandinger af alfa-amylaser og deres anvendelse til stivelsesforflydning
DK39693D0 (da) Enzym
DE69532283D1 (de) EIN PILZ, IN DEM DAS areA-GEN VERÄNDERT IST, UND EIN areA-GEN AUS ASPERGILLUS ORYZAE
MX9804263A (es) Expresion de genes incrementada por intensificador en plantas.
DE69427749T2 (de) Festes substrat mit hydrophilen reaktiven gruppen an der oberfläche
AR053946A2 (es) Variante de alfa-amilasa de bacillus stearothermophilus original
DE69326250D1 (de) Neuropeptide y antagoniste
ATE242318T1 (de) Rhamnogalacturonan acetylesterase (rgae) von aspergillus aculeatus
SE9702680D0 (sv) New formulation
FI952094A (fi) Ihmisen serotoniinireseptoria (5-HT4B)koodaava DNA ja sen käyttö
AU5588094A (en) The pct-65 serotonin receptor
DE69433238D1 (de) Pflanzenpromoter, microorganismen und pflanzenzellen mit einer expressionseinheit für ein gewünschtes protein, das besagten promoter enthält
ATE314481T1 (de) Promotor des transaldolase-genes von k.lactis und dessen verwendung
ATE124271T1 (de) Selbstklebendes matrixsystem für transdermal gesteuerte abgabe von piribedil.
ATE213742T1 (de) Zyklische somatostatinanaloge mit wachstumhormon inhibierender aktivität
DE69526087D1 (de) Or-1, ein wais-rezeptor der nuklearen rezeptoren familie
ATE148744T1 (de) Neue thrombininhibitorische proteine aus landblutegeln
GB9609261D0 (en) Gene expression in monocytes and microphages
ES2109373T3 (es) Nueva proteina inhibidora de trombina a partir de garrapatas.
DK0640139T3 (da) Fremgangsmåde til fremstilling af rekombinante proteiner i streptomyces

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties