FI942228A - Bacillus licheniformiksen x-amylaasipromoottorin variantista johdettu Bacillus-promoottori - Google Patents

Bacillus licheniformiksen x-amylaasipromoottorin variantista johdettu Bacillus-promoottori Download PDF

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Publication number
FI942228A
FI942228A FI942228A FI942228A FI942228A FI 942228 A FI942228 A FI 942228A FI 942228 A FI942228 A FI 942228A FI 942228 A FI942228 A FI 942228A FI 942228 A FI942228 A FI 942228A
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FI
Finland
Prior art keywords
bacillus
promoter
variant
pct
amylase
Prior art date
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FI942228A
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English (en)
Swedish (sv)
Other versions
FI942228A0 (fi
FI112668B (fi
Inventor
Steen Troels Joergensen
Boerge Krag Diderichsen
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Novo Nordisk As
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Publication of FI942228A0 publication Critical patent/FI942228A0/fi
Publication of FI942228A publication Critical patent/FI942228A/fi
Application granted granted Critical
Publication of FI112668B publication Critical patent/FI112668B/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
FI942228A 1991-11-14 1994-05-13 Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta FI112668B (fi)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK9100343 1991-11-14
DK9100343 1991-11-14
DK9200338 1992-11-13
PCT/DK1992/000338 WO1993010249A1 (en) 1991-11-14 1992-11-13 A bacillus promoter derived from a variant of a bacillus licheniformis x-amylase promoter

Publications (3)

Publication Number Publication Date
FI942228A0 FI942228A0 (fi) 1994-05-13
FI942228A true FI942228A (fi) 1994-07-13
FI112668B FI112668B (fi) 2003-12-31

Family

ID=8153698

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942228A FI112668B (fi) 1991-11-14 1994-05-13 Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta

Country Status (11)

Country Link
US (1) US5698415A (fi)
EP (1) EP0667910B1 (fi)
JP (1) JP3529774B2 (fi)
AT (1) ATE248223T1 (fi)
AU (1) AU661070B2 (fi)
CA (1) CA2123496C (fi)
DE (1) DE69233178T2 (fi)
DK (1) DK0667910T3 (fi)
FI (1) FI112668B (fi)
IL (1) IL103750A0 (fi)
WO (1) WO1993010249A1 (fi)

Families Citing this family (38)

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Publication number Priority date Publication date Assignee Title
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
CN1659283A (zh) 2002-04-10 2005-08-24 诺维信公司 地衣芽孢杆菌的突变宿主细胞
EP1497431A2 (en) 2002-04-10 2005-01-19 Novozymes A/S Improved bacillus host cell
EP1625208A4 (en) 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
US20100129862A1 (en) 2003-05-12 2010-05-27 Jones Brian E Novel lipolytic Enzyme lip1
US20060236414A1 (en) 2003-06-19 2006-10-19 Novozymes A/S Proteases and methods for producing them
AU2005230844A1 (en) * 2004-03-31 2005-10-20 Novozymes Biopharma Dk A/S Methods for producing hyaluronic acid in a bacillus cell
EP1766002B1 (en) 2004-06-21 2015-11-18 Novozymes A/S Stably maintained multiple copies of at least two orf in the same orientation
CN101611145B (zh) 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
BRPI0809096A2 (pt) 2007-03-23 2014-09-09 Danisco Us Inc Genecor Division Produção aumentada de amilase através da adição n-terminal à proteína amilase madura
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
BRPI0909373A2 (pt) 2008-03-28 2015-08-04 Danisco Us Inc Método para amplificação do locus em células bacterianas
US20110306106A1 (en) 2009-01-16 2011-12-15 Otsuka Chemical Co., Ltd High expression promoter and method for producing gene product using same
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011000924A1 (en) 2009-07-03 2011-01-06 Abbott Products Gmbh Spray-dried amylase, pharmaceutical preparations comprising the same and use
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
US9428558B2 (en) 2011-03-23 2016-08-30 Novozymes A/S Methods for producing secreted polypeptides
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
CN114127256A (zh) 2019-02-20 2022-03-01 巴斯夫欧洲公司 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺
WO2020229191A1 (en) 2019-05-15 2020-11-19 Novozymes A/S Temperature-sensitive rna-guided endonuclease
CN114207125A (zh) 2019-06-25 2022-03-18 诺维信公司 通过抑制条件性必需基因进行反向选择
BR112023001024A2 (pt) 2020-07-24 2023-02-14 Basf Se Método para produzir pelo menos um polipeptídeo de interesse, célula hospedeira de bacillus, e, caldo de fermentação
BR112023001386A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus
BR112023001392A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para produzir uma proteína de interesse a partir de uma célula hospedeira de bacillus
US20230295681A1 (en) 2020-07-28 2023-09-21 Basf Se Industrial fermentation process for bacillus using temperature shift
BR112023005111A2 (pt) 2020-09-22 2023-04-18 Basf Se Método para recuperar uma proteína de interesse de um caldo de fermentação bacteriana, e, uso de pelo menos um floculante
US20240060110A1 (en) 2020-10-07 2024-02-22 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
WO2022129166A1 (en) 2020-12-15 2022-06-23 Novozymes A/S Mutated host cells with reduced cell motility
EP4359424A1 (en) 2021-06-24 2024-05-01 Basf Se Improved bacillus production host
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus
WO2024028340A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells with salt feed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4769327A (en) * 1985-03-29 1988-09-06 Biotechnica International, Inc. Secretion vector

Also Published As

Publication number Publication date
FI942228A0 (fi) 1994-05-13
EP0667910A1 (en) 1995-08-23
AU661070B2 (en) 1995-07-13
AU2943092A (en) 1993-06-15
CA2123496C (en) 2004-02-03
EP0667910B1 (en) 2003-08-27
CA2123496A1 (en) 1993-05-27
DE69233178D1 (de) 2003-10-02
JP3529774B2 (ja) 2004-05-24
JPH07504085A (ja) 1995-05-11
IL103750A0 (en) 1993-04-04
WO1993010249A1 (en) 1993-05-27
DK0667910T3 (da) 2003-12-01
DE69233178T2 (de) 2004-06-17
ATE248223T1 (de) 2003-09-15
FI112668B (fi) 2003-12-31
US5698415A (en) 1997-12-16

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