BR112023001386A2 - Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus - Google Patents

Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus

Info

Publication number
BR112023001386A2
BR112023001386A2 BR112023001386A BR112023001386A BR112023001386A2 BR 112023001386 A2 BR112023001386 A2 BR 112023001386A2 BR 112023001386 A BR112023001386 A BR 112023001386A BR 112023001386 A BR112023001386 A BR 112023001386A BR 112023001386 A2 BR112023001386 A2 BR 112023001386A2
Authority
BR
Brazil
Prior art keywords
host cell
bacillus host
rates
cultivation
cultivating
Prior art date
Application number
BR112023001386A
Other languages
English (en)
Inventor
Daub Andreas
Golabgir Anbarani Aydin
Klein Tobias
Morweiser Michael
Benjamin Wandrey Georg
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of BR112023001386A2 publication Critical patent/BR112023001386A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)

Abstract

MÉTODO PARA CULTIVAR UMA CÉLULA HOSPEDEIRA DE BACILLUS, E, CULTURA DE CÉLULAS HOSPEDEIRAS DE BACILLUS. A presente invenção refere-se ao campo da fermentação industrial. Em particular, refere-se ao método para cultivar uma célula hospedeira de Bacillus compreendendo as etapas de (a) inocular um meio de fermentação com uma célula hospedeira de Bacillus compreendendo um construto de expressão para um gene que codifica uma proteína de interesse, cultivar, para uma primeira fase de cultivo, a célula hospedeira de Bacillus no dito meio de fermentação em condições favoráveis para o crescimento da célula hospedeira de Bacillus e da expressão da proteína de interesse, em que o cultivo da célula hospedeira de Bacillus compreende a adição de pelo menos uma solução de alimentação, e em que a pelo menos uma alimentação solução provê uma fonte de carbono em taxas crescentes, e (c) cultivar, para uma segunda fase de cultivo, a cultura de células hospedeiras de Bacillus obtida na etapa (b) em condições favoráveis para o crescimento da célula hospedeira de Bacillus e da expressão da proteína de interesse, em que o cultivo compreende a adição de pelo menos uma solução de alimentação, e em que a pelo menos uma solução de alimentação provê uma fonte de carbono em uma taxa constante, em taxas decrescentes ou em taxas que aumentam menos que as taxas na etapa (b), em que a dita taxa constante ou a taxa inicial das ditas taxas decrescentes ou a taxa inicial das ditas taxas que aumentam menos que as taxas na etapa (b) está abaixo da taxa máxima da primeira fase de cultivo. É adicionalmente contemplada uma cultura de células hospedeiras de Bacillus que pode ser obtida pelo dito método.
BR112023001386A 2020-07-28 2021-07-27 Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus BR112023001386A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20188162 2020-07-28
PCT/EP2021/071058 WO2022023372A1 (en) 2020-07-28 2021-07-27 Industrial fermentation process for bacillus using feed rate shift

Publications (1)

Publication Number Publication Date
BR112023001386A2 true BR112023001386A2 (pt) 2023-02-14

Family

ID=72145210

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023001386A BR112023001386A2 (pt) 2020-07-28 2021-07-27 Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus

Country Status (8)

Country Link
US (1) US20230287379A1 (pt)
EP (1) EP4189062A1 (pt)
KR (1) KR20230042509A (pt)
CN (1) CN116234922A (pt)
BR (1) BR112023001386A2 (pt)
CA (1) CA3186916A1 (pt)
MX (1) MX2023001273A (pt)
WO (1) WO2022023372A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480359B (zh) * 2022-03-22 2023-04-07 河南中大恒源生物科技股份有限公司 一种高密度发酵生产阿洛酮糖3-差向异构酶的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
EP0667910B1 (en) 1991-11-14 2003-08-27 Novozymes A/S A bacillus promoter derived from a variant of a bacillus licheniformis alpha-amylase promoter
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
EP1520011A2 (en) 2002-07-01 2005-04-06 Novozymes A/S Sterilisation of a fermentation medium comprising hydrolysed n-source
CA2563173A1 (en) 2004-03-31 2005-10-20 Novozymes Biopolymer A/S Methods for producing hyaluronic acid in a bacillus cell
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process
EP3102669B1 (en) 2014-02-07 2019-06-12 DSM IP Assets B.V. Improved bacillus host

Also Published As

Publication number Publication date
US20230287379A1 (en) 2023-09-14
WO2022023372A1 (en) 2022-02-03
CA3186916A1 (en) 2022-02-03
MX2023001273A (es) 2023-03-03
KR20230042509A (ko) 2023-03-28
EP4189062A1 (en) 2023-06-07
CN116234922A (zh) 2023-06-06

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