MX2023001273A - Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion. - Google Patents
Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion.Info
- Publication number
- MX2023001273A MX2023001273A MX2023001273A MX2023001273A MX2023001273A MX 2023001273 A MX2023001273 A MX 2023001273A MX 2023001273 A MX2023001273 A MX 2023001273A MX 2023001273 A MX2023001273 A MX 2023001273A MX 2023001273 A MX2023001273 A MX 2023001273A
- Authority
- MX
- Mexico
- Prior art keywords
- host cell
- bacillus host
- rates
- bacillus
- feed solution
- Prior art date
Links
- 241000193830 Bacillus <bacterium> Species 0.000 title abstract 9
- 230000004151 fermentation Effects 0.000 title abstract 3
- 238000009655 industrial fermentation Methods 0.000 title abstract 2
- 239000012527 feed solution Substances 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 2
- 238000000855 fermentation Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
Abstract
La presente invención se refiere al campo de la fermentación industrial. En particular, se refiere a un proceso para cultivar una célula huésped de Bacillus que comprende las etapas de (a) inocular un medio de fermentación con una célula huésped de Bacillus que comprende un constructo de expresión para un gen que codifica una proteína de interés, cultivar durante una primera fase de cultivo la célula huésped de Bacillus en dicho medio de fermentación en condiciones propicias para el crecimiento de la célula huésped de Bacillus y la expresión de la proteína de interés, en donde el cultivo de la célula huésped de Bacillus comprende la adición de al menos una solución de alimentación y en donde la al menos una solución de alimentación proporciona una fuente de carbono a velocidades en aumento, y (c) cultivar durante una segunda fase de cultivo el cultivo de la célula huésped de Bacillus obtenido en la etapa (b) en condiciones propicias para el crecimiento de la célula huésped de Bacillus y la expresión de la proteína de interés, en donde el cultivo comprende la adición de al menos una solución de alimentación y en donde la al menos una solución de alimentación proporciona una fuente de carbono a una velocidad constante, a velocidades disminuidas o a velocidades que aumentan menos que las velocidades en la etapa (b), en donde dicha velocidad constante o la velocidad inicial de dichas velocidades o la velocidad inicial de dichas velocidades que aumenta menos que las velocidades en la etapa (b) está por debajo de la velocidad máxima de la primera fase de cultivo. Se contempla además un cultivo de células huésped de Bacillus que se puede obtener mediante dicho proceso.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20188162 | 2020-07-28 | ||
| PCT/EP2021/071058 WO2022023372A1 (en) | 2020-07-28 | 2021-07-27 | Industrial fermentation process for bacillus using feed rate shift |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2023001273A true MX2023001273A (es) | 2023-03-03 |
Family
ID=72145210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2023001273A MX2023001273A (es) | 2020-07-28 | 2021-07-27 | Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20230287379A1 (es) |
| EP (1) | EP4189062A1 (es) |
| KR (1) | KR20230042509A (es) |
| CN (1) | CN116234922A (es) |
| BR (1) | BR112023001386A2 (es) |
| CA (1) | CA3186916A1 (es) |
| MX (1) | MX2023001273A (es) |
| WO (1) | WO2022023372A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114480359B (zh) * | 2022-03-22 | 2023-04-07 | 河南中大恒源生物科技股份有限公司 | 一种高密度发酵生产阿洛酮糖3-差向异构酶的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK639689D0 (da) | 1989-12-18 | 1989-12-18 | Novo Nordisk As | Indfoering af dna i celler |
| DK0667910T3 (da) | 1991-11-14 | 2003-12-01 | Novozymes As | En Bacillus-promoter, afledt fra en variant af en alpha-amylase-promoter fra Bacillus licheniformis |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| AU2003236810A1 (en) | 2002-07-01 | 2004-01-19 | Novozymes A/S | Sterilization of a fermentation medium comprising hydrolysed n-source |
| CN101426925A (zh) | 2004-03-31 | 2009-05-06 | 诺维信生物聚合物公司 | 在芽孢杆菌细胞中生产透明质酸的方法 |
| EP2292769A1 (en) * | 2009-08-10 | 2011-03-09 | Lonza AG | Fermentation process |
| CN105992817B (zh) | 2014-02-07 | 2020-09-11 | 帝斯曼知识产权资产管理有限公司 | 改良的芽孢杆菌宿主 |
-
2021
- 2021-07-27 MX MX2023001273A patent/MX2023001273A/es unknown
- 2021-07-27 EP EP21752665.6A patent/EP4189062A1/en active Pending
- 2021-07-27 CA CA3186916A patent/CA3186916A1/en active Pending
- 2021-07-27 CN CN202180059654.XA patent/CN116234922A/zh active Pending
- 2021-07-27 KR KR1020237007068A patent/KR20230042509A/ko unknown
- 2021-07-27 US US18/018,094 patent/US20230287379A1/en active Pending
- 2021-07-27 WO PCT/EP2021/071058 patent/WO2022023372A1/en active Application Filing
- 2021-07-27 BR BR112023001386A patent/BR112023001386A2/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112023001386A2 (pt) | 2023-02-14 |
| CA3186916A1 (en) | 2022-02-03 |
| CN116234922A (zh) | 2023-06-06 |
| KR20230042509A (ko) | 2023-03-28 |
| EP4189062A1 (en) | 2023-06-07 |
| US20230287379A1 (en) | 2023-09-14 |
| WO2022023372A1 (en) | 2022-02-03 |
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