DE69221086D1 - Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen - Google Patents

Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen

Info

Publication number
DE69221086D1
DE69221086D1 DE69221086T DE69221086T DE69221086D1 DE 69221086 D1 DE69221086 D1 DE 69221086D1 DE 69221086 T DE69221086 T DE 69221086T DE 69221086 T DE69221086 T DE 69221086T DE 69221086 D1 DE69221086 D1 DE 69221086D1
Authority
DE
Germany
Prior art keywords
cells
cell
hemoglobin
reagent compositions
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69221086T
Other languages
English (en)
Other versions
DE69221086T2 (de
Inventor
Sophie S Fan
Daniel Ben-David
Albert Cupo
Gena Fischer
Grace E Martin
Leonard Ornstein
Gregory M Colella
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Icahn School of Medicine at Mount Sinai
Original Assignee
Bayer AG
Bayer Corp
Mount Sinai School of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp, Mount Sinai School of Medicine filed Critical Bayer AG
Publication of DE69221086D1 publication Critical patent/DE69221086D1/de
Application granted granted Critical
Publication of DE69221086T2 publication Critical patent/DE69221086T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5002—Partitioning blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/102499—Blood gas standard or control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE69221086T 1991-12-05 1992-11-27 Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen Expired - Lifetime DE69221086T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/802,674 US5284771A (en) 1991-12-05 1991-12-05 Reagent compositions and their use in sphering cells

Publications (2)

Publication Number Publication Date
DE69221086D1 true DE69221086D1 (de) 1997-09-04
DE69221086T2 DE69221086T2 (de) 1997-11-13

Family

ID=25184391

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69221086T Expired - Lifetime DE69221086T2 (de) 1991-12-05 1992-11-27 Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen

Country Status (12)

Country Link
US (2) US5284771A (de)
EP (1) EP0545313B1 (de)
JP (1) JP2711786B2 (de)
KR (1) KR100276145B1 (de)
AT (1) ATE155887T1 (de)
AU (1) AU661728B2 (de)
CA (1) CA2077790C (de)
DE (1) DE69221086T2 (de)
DK (1) DK0545313T3 (de)
ES (1) ES2104802T3 (de)
IL (1) IL103056A (de)
TW (1) TW265412B (de)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585246A (en) * 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
EP0698211B1 (de) * 1993-05-14 2003-04-02 Coulter International Corporation Retikulozyt bestimmungsverfahren und geraet, das lichtstreuungstechniken verwendet
US5691204A (en) * 1995-04-21 1997-11-25 Abbott Laboratories Compositions and methods for the rapid analysis of reticulocytes
JP3425830B2 (ja) * 1995-10-06 2003-07-14 シスメックス株式会社 新規化合物とその用途
US5733784A (en) * 1995-11-20 1998-03-31 Abbott Laboratories Reagent system and method for the differentiation and identification of reticulocytes
US6025201A (en) * 1995-12-28 2000-02-15 Bayer Corporation Highly sensitive, accurate, and precise automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples
US5817519A (en) * 1995-12-28 1998-10-06 Bayer Corporation Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples
TW379284B (en) * 1996-04-12 2000-01-11 Toa Medical Electronics Agent for detecting reticulocyte
US5830764A (en) * 1996-11-12 1998-11-03 Bayer Corporation Methods and reagent compositions for the determination of membrane surface area and sphericity of erythrocytes and reticulocytes for the diagnosis of red blood cell disorders
FR2759166B1 (fr) * 1997-01-31 1999-04-23 Abx Sa Reactif de coloration pour la determination de cellules sanguines
US6114173A (en) * 1997-04-03 2000-09-05 Bayer Corporation Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
JPH10307135A (ja) * 1997-05-02 1998-11-17 Toa Medical Electronics Co Ltd 赤血球形態異常の検出方法
US6060322A (en) * 1998-10-20 2000-05-09 Coulter International Corp. Method for identification of reticulated cells
US6271035B1 (en) * 1998-10-20 2001-08-07 Coulter International Corp. Methods and compositions for rapid staining of nucleic acids in whole cells
IL145669A0 (en) * 1999-03-31 2002-06-30 Bayer Ag Single channel, single dilution detection method
US6582574B1 (en) * 1999-03-31 2003-06-24 City Of Hope pK-matched running buffers for gel electrophoresis
US6190919B1 (en) * 1999-04-21 2001-02-20 The United States Of America As Represented By The Secretary Of The Navy System for controlling deglycerolization of red blood cells
US6468732B1 (en) * 2000-04-04 2002-10-22 Bayer Corporation Method and long-term stable bicarbonate-containing diluent composition, and storage means therefor, for reducing or reversing aeration induced cell shrinkage and storage induced cell swelling of a whole blood sample
US6368864B1 (en) 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
US6784981B1 (en) * 2000-06-02 2004-08-31 Idexx Laboratories, Inc. Flow cytometry-based hematology system
JP4167491B2 (ja) * 2001-03-09 2008-10-15 株式会社三菱化学ヤトロン 全血測定法
US6630990B2 (en) 2001-06-05 2003-10-07 Abbott Laboratories Optical method and apparatus for red blood cell differentiation on a cell-by-cell basis, and simultaneous analysis of white blood cell differentiation
CA2428740A1 (en) 2002-05-20 2003-11-20 Bayer Corporation Automated method and reagent therefor for assaying body fluid samples such as cerebrospinal fluid (csf)
DE102004006182B4 (de) * 2003-08-19 2007-08-16 Biotronix Gmbh Verfahren zur elektrooptischen Analyse von Zellsuspensionen
US7674598B2 (en) * 2004-05-21 2010-03-09 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
US7625712B2 (en) 2004-05-21 2009-12-01 Beckman Coulter, Inc. Method for a fully automated monoclonal antibody-based extended differential
FR2883971B1 (fr) * 2005-03-31 2007-11-16 C2 Diagnostics Sa Dispositif optique d'analyse sanguine, appareil d'analyse equipe d'un tel dispositif
JP2007147476A (ja) * 2005-11-29 2007-06-14 Nidec Sankyo Corp 光散乱式粒子計数装置
CN101349644B (zh) * 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
US8102161B2 (en) * 2007-09-25 2012-01-24 Tdk Corporation Stable output in a switching power supply by smoothing the output of the secondary coil
WO2009058876A1 (en) * 2007-10-29 2009-05-07 Beckman Coulter, Inc. Method for a rapid antibody-based analysis of platelet populations
US8159670B2 (en) * 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
CN101475754A (zh) * 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
US20090314510A1 (en) * 2008-01-11 2009-12-24 Kukowski Thomas R Elastomeric Conductors and Shields
CN103823051B (zh) * 2008-03-21 2015-11-18 艾博特健康公司 利用红细胞内含有的血红蛋白的本征色素沉着来确定血样的红细胞指数的方法及设备
CN102016578B (zh) 2008-03-21 2014-10-01 艾博特健康公司 利用红细胞内含有的血红蛋白的本征色素沉着来确定血样的血细胞比容的方法及设备
JP4887464B2 (ja) 2008-03-21 2012-02-29 アボット ポイント オブ ケア インコーポレイテッド 生体試料を撮像するように構成された撮像機器の焦点位置を決定するための方法及び装置
EP3109621A1 (de) 2008-03-21 2016-12-28 Abbott Point Of Care, Inc. Vorrichtung zur analyse einzelner zellen oder partikeln in einer blutprobe auf der grundlage von fluoreszenzbildern und bildern der optischen dichte der probe
CN109239321A (zh) * 2008-03-21 2019-01-18 艾博特健康公司 单独和在聚合凝块中检测和计数血小板的方法及设备
CN102027370B (zh) * 2008-04-02 2016-06-08 艾博特健康公司 用于对包括全血的生物流体执行免疫测定的配体分析中的结合标记和游离标记的虚拟分离
EP2281197B1 (de) * 2008-04-09 2015-09-02 Abbott Point Of Care, Inc. Verfahren zum nachweisen sehr geringer analytniveaus in einer dünnschichtkammer enthaltenen dünnfilmfluidprobe
US8326008B2 (en) * 2008-04-09 2012-12-04 Abbott Point Of Care, Inc. Method for measuring the area of a sample disposed within an analysis chamber
CN101602762B (zh) * 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
CN101726579B (zh) * 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
CN101750274B (zh) * 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
JP4969596B2 (ja) * 2009-02-04 2012-07-04 シスメックス株式会社 試料分析装置、試料分析方法およびプログラム
US20100255605A1 (en) * 2009-04-02 2010-10-07 Abbott Point Of Care, Inc. Method and device for transferring biologic fluid samples
WO2010126838A1 (en) 2009-04-27 2010-11-04 Abbott Laboratories Method for discriminating red blood cells from white blood cells by using forward scattering from a laser in an automated hematology analyzer
CN101988082B (zh) * 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
ES2438841T3 (es) * 2009-12-31 2014-01-20 Abbott Point Of Care, Inc. Método y aparato para determinar el volumen celular medio de los glóbulos rojos en la sangre
US8906308B2 (en) 2010-01-15 2014-12-09 Abbott Laboratories Method for determining volume and hemoglobin content of individual red blood cells
WO2011116305A1 (en) 2010-03-18 2011-09-22 Abbott Point Of Care, Inc. Method and apparatus for optically determining at least one hemoglobin related parameter of a whole blood sample
DE102011003101B4 (de) 2010-12-23 2016-04-07 Siemens Healthcare Diagnostics Products Gmbh Verfahren zum Nachweis einer Plasmodieninfektion
JP6316933B2 (ja) * 2014-02-28 2018-04-25 シスメックス株式会社 尿試料分析方法、尿試料分析用試薬及び尿試料分析用試薬キット
CN105334191B (zh) * 2014-07-25 2020-09-08 深圳迈瑞生物医疗电子股份有限公司 单个红细胞的血红蛋白浓度、体积修正方法及装置
EP3268737A4 (de) 2015-03-10 2018-11-14 Alentic Microscience Inc. Verbesserte probenverarbeitung für quantitative mikroskopie
US9863864B2 (en) 2015-09-15 2018-01-09 Bio-Rad Technologies, Inc. Threshold selector for flow cytometer
FR3073047B1 (fr) 2017-11-02 2021-01-29 Commissariat Energie Atomique Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon
AU2019242902B2 (en) 2018-03-30 2021-10-28 Idexx Laboratories, Inc. Laser optics assembly of flow cytometer
WO2020190641A1 (en) * 2019-03-21 2020-09-24 Becton, Dickinson And Company Light detection systems and methods of use thereof
US11543342B2 (en) 2020-06-17 2023-01-03 Idexx Laboratories, Inc. Flow cytometer and laser optics assembly thereof

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3684377A (en) * 1970-07-13 1972-08-15 Bio Physics Systems Inc Method for analysis of blood by optical analysis of living cells
US3883247A (en) * 1973-10-30 1975-05-13 Bio Physics Systems Inc Method for fluorescence analysis of white blood cells
US4346018A (en) * 1980-06-16 1982-08-24 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4336029A (en) * 1980-08-15 1982-06-22 Ortho Diagnostic Systems Inc. Method and reagents for quantitative determination of reticulocytes and platelets in whole blood
US4325706A (en) * 1980-08-15 1982-04-20 Ortho Diagnostic Systems Inc. Automated detection of platelets and reticulocytes in whole blood
US4412004A (en) * 1981-06-26 1983-10-25 Technicon Instruments Corporation Method for treating red blood cells to effect sphering and reagent therefor
US4490353A (en) * 1983-07-13 1984-12-25 Colgate-Palmolive Company Antiplaque dentifrice with improved fluoride stability
US4735504A (en) * 1983-10-31 1988-04-05 Technicon Instruments Corporation Method and apparatus for determining the volume & index of refraction of particles
US4575490A (en) * 1984-02-29 1986-03-11 Technicon Instruments Corporation One step method for sphering and fixing whole blood erythrocytes
DE3587636T2 (de) * 1984-05-31 1994-03-10 Coulter Corp Reagenssystem und verfahren zum nachweis, aufzählen und prüfen der klassen und unterklassen von blutleukozyten.
US4652517A (en) * 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
JPS6179163A (ja) * 1984-09-26 1986-04-22 Toa Medical Electronics Co Ltd 血球測定用試薬
JPS61280565A (ja) * 1985-06-06 1986-12-11 Toa Medical Electronics Co Ltd フロ−サイトメトリ−用網状血球測定試薬
US4707451A (en) * 1985-09-18 1987-11-17 Becton, Dickinson And Company Detection of reticulocytes
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US5039613A (en) * 1986-11-27 1991-08-13 Toa Medical Electronics Co., Ltd. Reagents used in a method of classifying leukocytes by flow cytometry
US4853338A (en) * 1987-05-20 1989-08-01 Technicon Instruments Corporation Cyanide-free hemoglobin reagent
JPH0746102B2 (ja) * 1987-07-31 1995-05-17 東亜医用電子株式会社 フロ−サイトメトリ用網状赤血球測定試薬
JP2549665B2 (ja) * 1987-07-31 1996-10-30 東亜医用電子株式会社 フロ−サイトメトリ用網状赤血球測定試薬
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5242832A (en) * 1990-03-01 1993-09-07 Toa Medical Electronics Co., Ltd. Reagent for measurement of leukocytes and hemoglobin in blood

Also Published As

Publication number Publication date
ES2104802T3 (es) 1997-10-16
CA2077790C (en) 2002-07-02
KR100276145B1 (ko) 2000-12-15
CA2077790A1 (en) 1993-06-06
ATE155887T1 (de) 1997-08-15
DK0545313T3 (da) 1998-02-16
US5633167A (en) 1997-05-27
JP2711786B2 (ja) 1998-02-10
IL103056A (en) 1997-04-15
TW265412B (de) 1995-12-11
US5284771A (en) 1994-02-08
EP0545313B1 (de) 1997-07-23
DE69221086T2 (de) 1997-11-13
EP0545313A1 (de) 1993-06-09
AU2816692A (en) 1993-06-10
IL103056A0 (en) 1993-02-21
JPH06180316A (ja) 1994-06-28
AU661728B2 (en) 1995-08-03

Similar Documents

Publication Publication Date Title
DE69221086D1 (de) Reagenzzusammensetzungen und ihre Verwendung zur Erzeugung von kugelförmigen roten Blutzellen
TW287232B (de)
IL103055A0 (en) Methods and reagent compositions for characterizing and identifying reticulocytes in whole blood
EP0869347A3 (de) Verfahren und Reagent zur Identifikation von Retikulozyten, Erythrozyten und Blutplättchen
DE69922355D1 (de) Analyse von ruhenden mit antikoagulans behandelten vollblutproben
EP1542008A4 (de) Verfahren zur klassifizierung und zählung von leukozyten
PT95928A (pt) Processo para a preparacao de um regente utilizado na determinacao automatica, em citometria de fluxo, de, pelo menos, uma subpopulacao leucocitaria a partir de sangue total
CN1323395A (zh) 鉴别网状细胞的试剂组合物和方法
Schneider et al. Identification of hemoglobins and hemoglobinopathies by electrophoresis on cellulose acetate plates impregnated with citrate agar
Garruto et al. Lack of prominent compensatory polycythemia in traditional native Andeans living at 4,200 meters
DE69629938D1 (de) Reagent-system und verfahren für differentiation und identifikation von retikulocyten
JP2009080122A (ja) 赤芽球の分類計数方法
WO1999027346A8 (en) Method for differentiation of reticulocytes in blood
Molden et al. Fetal hemoglobin: optimum conditions for its estimation by alkali denaturation
ATE142332T1 (de) Verfahren zum sichten von zellen oder geformten körpern mit ausgewählten eigenschaften unter benutzung eines nachweisparameters
Van Pelt Characteristics of normal equine tarsal synovial fluid
ES481548A1 (es) Metodo para preparar un control de referencia hematologico.
Wadsworth et al. Haematological, coagulation and blood chemistry data in red-bellied tamarins Saguinus labiatus
Hendry Physical and chemical factors concerned in haemolysis

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
R071 Expiry of right

Ref document number: 545313

Country of ref document: EP