DE69130698T2 - Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt - Google Patents
Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem KohlenhydratgehaltInfo
- Publication number
- DE69130698T2 DE69130698T2 DE69130698T DE69130698T DE69130698T2 DE 69130698 T2 DE69130698 T2 DE 69130698T2 DE 69130698 T DE69130698 T DE 69130698T DE 69130698 T DE69130698 T DE 69130698T DE 69130698 T2 DE69130698 T2 DE 69130698T2
- Authority
- DE
- Germany
- Prior art keywords
- methods
- production
- transgenic plants
- carbohydrate content
- modified carbohydrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Compounds Of Unknown Constitution (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP90202434 | 1990-09-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69130698D1 DE69130698D1 (de) | 1999-02-11 |
DE69130698T2 true DE69130698T2 (de) | 1999-07-22 |
Family
ID=8205119
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69130698T Expired - Lifetime DE69130698T2 (de) | 1990-09-13 | 1991-09-13 | Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt |
Country Status (13)
Country | Link |
---|---|
US (1) | US20060195940A1 (de) |
EP (1) | EP0479359B1 (de) |
JP (1) | JP3938402B2 (de) |
AT (1) | ATE175238T1 (de) |
AU (1) | AU656920B2 (de) |
CA (1) | CA2072656C (de) |
DE (1) | DE69130698T2 (de) |
DK (1) | DK0479359T3 (de) |
IE (1) | IE913215A1 (de) |
IL (1) | IL99482A (de) |
NZ (1) | NZ239789A (de) |
PT (1) | PT98967B (de) |
WO (1) | WO1992005259A1 (de) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4035756A1 (de) * | 1990-11-08 | 1992-05-14 | Inst Genbiologische Forschung | Neue plasmide zur herstellung von im habitus und ertrag veraenderten transgenen pflanzen |
WO1995004826A1 (en) * | 1993-08-09 | 1995-02-16 | Institut Für Genbiologische Forschung Berlin Gmbh | Debranching enzymes and dna sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants |
DE4327165A1 (de) | 1993-08-09 | 1995-02-16 | Inst Genbiologische Forschung | Debranching-Enzyme und deren kodierende DNA-Sequenzen, geeignet zur Veränderung des Verzweigungsgrades von Amylopektin - Stärke in Pflanzen |
GB9320930D0 (en) * | 1993-10-12 | 1993-12-01 | Zeneca Ltd | Modified fruit |
GB9403423D0 (en) * | 1994-02-23 | 1994-04-13 | Unilever Plc | Novel exo-(1-4)- beta-D galactanase |
US5767373A (en) | 1994-06-16 | 1998-06-16 | Novartis Finance Corporation | Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms |
US5750876A (en) * | 1994-07-28 | 1998-05-12 | Monsanto Company | Isoamylase gene, compositions containing it, and methods of using isoamylases |
AU3395995A (en) * | 1994-09-09 | 1996-03-27 | Unilever Plc | Xyloglucan-specific beta-galactosidase |
GB9421287D0 (en) * | 1994-10-21 | 1994-12-07 | Danisco | A method of reducing the level of sugar in an organism |
GB9421286D0 (en) * | 1994-10-21 | 1994-12-07 | Danisco | Promoter |
DE4447387A1 (de) | 1994-12-22 | 1996-06-27 | Inst Genbiologische Forschung | Debranching-Enzyme aus Pflanzen und DNA-Sequenzen kodierend diese Enzyme |
GB9505479D0 (en) * | 1995-03-17 | 1995-05-03 | Danisco | Enzyme |
US6084155A (en) | 1995-06-06 | 2000-07-04 | Novartis Ag | Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes |
GB9522589D0 (en) * | 1995-11-03 | 1996-01-03 | Innes John Centre | Modified plants and plant products |
GB9526613D0 (en) * | 1995-12-28 | 1996-02-28 | Scottish Crop Research Inst | Sequence |
AU715778B2 (en) * | 1996-03-05 | 2000-02-10 | Friedrich Weissheimer Malzfabrik | Process for the production of degradation and/or conversion products of storage substances present in transgenic plant material with the help of a malting process |
DE19608918A1 (de) | 1996-03-07 | 1997-09-11 | Planttec Biotechnologie Gmbh | Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Mais codieren |
DE19618125A1 (de) | 1996-05-06 | 1997-11-13 | Planttec Biotechnologie Gmbh | Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Kartoffel codieren |
MXPA99001239A (es) | 1996-08-05 | 2003-09-12 | Syngenta Mogen Bv | Proceso mejorado para la produccion de bebidas alcoholicas que utilizan semilla de malta. |
ES2242234T3 (es) | 1996-09-12 | 2005-11-01 | Syngenta Participations Ag | Plantas transgenicas que expresan enzimas celuloliticas. |
EP1574580A3 (de) * | 1996-09-12 | 2009-07-01 | Syngenta Participations AG | Cellulolytische enzyme-exprimierende transgene Pflanzen |
US5981835A (en) * | 1996-10-17 | 1999-11-09 | Wisconsin Alumni Research Foundation | Transgenic plants as an alternative source of lignocellulosic-degrading enzymes |
US6818803B1 (en) | 1997-06-26 | 2004-11-16 | Wisconsin Alumni Research Foundation | Transgenic plants as an alternative source of lignocellulosic-degrading enzymes |
AU1016999A (en) * | 1997-11-07 | 1999-05-31 | University Of Guelph | Cdna clone of endo-beta-mannanase from plant tissues |
DE19820608A1 (de) * | 1998-05-08 | 1999-11-11 | Hoechst Schering Agrevo Gmbh | Nucleinsäuremoleküle codierend Enzyme aus Weizen, die an der Stärkesynthese beteiligt sind |
DE19836099A1 (de) * | 1998-07-31 | 2000-02-03 | Hoechst Schering Agrevo Gmbh | Nukleinsäuremoleküle kodierend für eine ß-Amylase, Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke |
DE19836097A1 (de) * | 1998-07-31 | 2000-02-03 | Hoechst Schering Agrevo Gmbh | Nukleinsäuremoleküle kodierend für eine alpha-Glukosidase, Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke |
US6940002B1 (en) | 1998-11-12 | 2005-09-06 | Novozymes A/S | Transgenic plant expressing maltogenic alpha-amylase |
WO2000029591A1 (en) * | 1998-11-12 | 2000-05-25 | Novozymes A/S | Transgenic plant expressing maltogenic alpha-amylase |
DE60238339D1 (de) * | 2001-08-27 | 2010-12-30 | Syngenta Participations Ag | Selbstprozessierende pflanzen und pflanzenteile |
EP1730284A4 (de) * | 2004-03-08 | 2008-04-30 | Syngenta Participations Ag | Selbstprozessierende pflanzen und pflanzenteile |
PT1868426T (pt) | 2005-03-16 | 2018-05-08 | Syngenta Participations Ag | Evento de milho 3272 e métodos para a sua deteção |
EP2444413A1 (de) | 2006-08-04 | 2012-04-25 | Verenium Corporation | Verfahren für Öl- oder Gas-Bohrungen, Fluten und/oder "Fracturing" |
DK2479266T3 (en) | 2006-12-21 | 2016-06-20 | Basf Enzymes Llc | Amylases and glucoamylases, nucleic acids encoding them, and methods of making and using the same |
US7915020B2 (en) | 2007-06-01 | 2011-03-29 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
WO2008150880A1 (en) | 2007-06-01 | 2008-12-11 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
US7727726B2 (en) | 2007-06-01 | 2010-06-01 | Syngenta Participations Ag | Process for starch liquefaction and fermentation |
WO2016079035A1 (en) | 2014-11-18 | 2016-05-26 | Bayer Cropscience Aktiengesellschaft | Use of certain herbicide combinations in tuberous root crop plants |
CN107529755A (zh) | 2015-04-27 | 2018-01-02 | 拜耳作物科学股份公司 | 某些除草剂结合物在块根作物植物中的用途 |
WO2016173972A1 (en) | 2015-04-27 | 2016-11-03 | Bayer Cropscience Aktiengesellschaft | Use of certain herbicide combinations in tuberous root crop plants |
CN104830898B (zh) * | 2015-05-15 | 2018-06-05 | 湖南工程学院 | 一种提高人参根中人参皂苷Rg1含量的方法与应用 |
WO2017050635A1 (en) | 2015-09-22 | 2017-03-30 | Bayer Cropscience Aktiengesellschaft | Use of certain active ingredient combinations in tuberous root crop plants |
CN115646867A (zh) * | 2018-06-30 | 2023-01-31 | 曹可瀚 | 一种汽车清洗装置和清洗方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE278791T1 (de) * | 1988-02-26 | 2004-10-15 | Large Scale Biology Corp | Nichtnukleare chromosomale transformation |
EP0428572A1 (de) * | 1988-07-29 | 1991-05-29 | Washington University School Of Medicine | Herstellung von kommerziell wertvollen polypeptiden durch genetisch transformierte endospermgewebe |
DD275704A1 (de) * | 1988-09-23 | 1990-01-31 | Akad Wissenschaften Ddr | Verfahren zur herstellung von gerstenpflanzen |
DK198089D0 (da) * | 1989-04-24 | 1989-04-24 | Danske Spritfabrikker | Dna-materialer og anvendelse deraf |
PT97110B (pt) * | 1990-03-23 | 1998-11-30 | Gist Brocades Nv | Processo para catalisar reaccoes acelaraveis por enzimas, mediante adicao ao meio reaccional de sementes de plantas transgenicas e para obtencao das referidas sementes |
US5543576A (en) * | 1990-03-23 | 1996-08-06 | Mogen International | Production of enzymes in seeds and their use |
US5705375A (en) * | 1990-09-13 | 1998-01-06 | Mogen International, N.V. | Transgenic plants having a modified carbohydrate content |
DE19516807C2 (de) * | 1995-05-08 | 1998-01-15 | Siegfried Mache | Vorrichtung zum Aufnehmen von kontinuierlich in einer Förderbahn einzeln hintereinander zugeführten Gegenständen und zum gruppenweisen Bereitstellen der Gegenstände |
-
1991
- 1991-09-12 IE IE321591A patent/IE913215A1/en not_active IP Right Cessation
- 1991-09-13 WO PCT/NL1991/000171 patent/WO1992005259A1/en active Application Filing
- 1991-09-13 PT PT98967A patent/PT98967B/pt not_active IP Right Cessation
- 1991-09-13 DK DK91202355T patent/DK0479359T3/da active
- 1991-09-13 NZ NZ239789A patent/NZ239789A/en not_active IP Right Cessation
- 1991-09-13 JP JP51745691A patent/JP3938402B2/ja not_active Expired - Lifetime
- 1991-09-13 CA CA002072656A patent/CA2072656C/en not_active Expired - Lifetime
- 1991-09-13 AT AT91202355T patent/ATE175238T1/de not_active IP Right Cessation
- 1991-09-13 DE DE69130698T patent/DE69130698T2/de not_active Expired - Lifetime
- 1991-09-13 EP EP91202355A patent/EP0479359B1/de not_active Expired - Lifetime
- 1991-09-13 AU AU86514/91A patent/AU656920B2/en not_active Expired
- 1991-09-15 IL IL9948291A patent/IL99482A/xx not_active IP Right Cessation
-
2005
- 2005-07-30 US US11/193,968 patent/US20060195940A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JPH05502591A (ja) | 1993-05-13 |
IE913215A1 (en) | 1992-02-25 |
ATE175238T1 (de) | 1999-01-15 |
NZ239789A (en) | 1994-06-27 |
IL99482A0 (en) | 1992-08-18 |
WO1992005259A1 (en) | 1992-04-02 |
AU656920B2 (en) | 1995-02-23 |
PT98967A (pt) | 1992-07-31 |
CA2072656C (en) | 2005-06-14 |
CA2072656A1 (en) | 1992-03-14 |
AU8651491A (en) | 1992-04-15 |
PT98967B (pt) | 1999-02-26 |
EP0479359B1 (de) | 1998-12-30 |
IL99482A (en) | 2005-12-18 |
JP3938402B2 (ja) | 2007-06-27 |
EP0479359A1 (de) | 1992-04-08 |
US20060195940A1 (en) | 2006-08-31 |
DE69130698D1 (de) | 1999-02-11 |
DK0479359T3 (da) | 1999-08-30 |
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