DE69130698T2 - Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt - Google Patents

Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt

Info

Publication number
DE69130698T2
DE69130698T2 DE69130698T DE69130698T DE69130698T2 DE 69130698 T2 DE69130698 T2 DE 69130698T2 DE 69130698 T DE69130698 T DE 69130698T DE 69130698 T DE69130698 T DE 69130698T DE 69130698 T2 DE69130698 T2 DE 69130698T2
Authority
DE
Germany
Prior art keywords
methods
production
transgenic plants
carbohydrate content
modified carbohydrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69130698T
Other languages
English (en)
Other versions
DE69130698D1 (de
Inventor
Den Elzen Peter Van
Jan Pen
Andreas Hoekema
Peter Christiaan Sijmons
Ooyen Albert J J Van
Krijn Rietveld
Wim Quax
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Mogen International NV
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mogen International NV, Gist Brocades NV filed Critical Mogen International NV
Application granted granted Critical
Publication of DE69130698D1 publication Critical patent/DE69130698D1/de
Publication of DE69130698T2 publication Critical patent/DE69130698T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
DE69130698T 1990-09-13 1991-09-13 Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt Expired - Lifetime DE69130698T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP90202434 1990-09-13

Publications (2)

Publication Number Publication Date
DE69130698D1 DE69130698D1 (de) 1999-02-11
DE69130698T2 true DE69130698T2 (de) 1999-07-22

Family

ID=8205119

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69130698T Expired - Lifetime DE69130698T2 (de) 1990-09-13 1991-09-13 Methoden zur Herstellung von transgenen Pflanzen mit modifiziertem Kohlenhydratgehalt

Country Status (13)

Country Link
US (1) US20060195940A1 (de)
EP (1) EP0479359B1 (de)
JP (1) JP3938402B2 (de)
AT (1) ATE175238T1 (de)
AU (1) AU656920B2 (de)
CA (1) CA2072656C (de)
DE (1) DE69130698T2 (de)
DK (1) DK0479359T3 (de)
IE (1) IE913215A1 (de)
IL (1) IL99482A (de)
NZ (1) NZ239789A (de)
PT (1) PT98967B (de)
WO (1) WO1992005259A1 (de)

Families Citing this family (43)

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DE4035756A1 (de) * 1990-11-08 1992-05-14 Inst Genbiologische Forschung Neue plasmide zur herstellung von im habitus und ertrag veraenderten transgenen pflanzen
DE4327165A1 (de) 1993-08-09 1995-02-16 Inst Genbiologische Forschung Debranching-Enzyme und deren kodierende DNA-Sequenzen, geeignet zur Veränderung des Verzweigungsgrades von Amylopektin - Stärke in Pflanzen
WO1995004826A1 (en) * 1993-08-09 1995-02-16 Institut Für Genbiologische Forschung Berlin Gmbh Debranching enzymes and dna sequences coding them, suitable for changing the degree of branching of amylopectin starch in plants
GB9320930D0 (en) * 1993-10-12 1993-12-01 Zeneca Ltd Modified fruit
GB9403423D0 (en) * 1994-02-23 1994-04-13 Unilever Plc Novel exo-(1-4)- beta-D galactanase
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5750876A (en) * 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
AU3395995A (en) * 1994-09-09 1996-03-27 Unilever Plc Xyloglucan-specific beta-galactosidase
GB9421287D0 (en) * 1994-10-21 1994-12-07 Danisco A method of reducing the level of sugar in an organism
GB9421286D0 (en) * 1994-10-21 1994-12-07 Danisco Promoter
DE4447387A1 (de) 1994-12-22 1996-06-27 Inst Genbiologische Forschung Debranching-Enzyme aus Pflanzen und DNA-Sequenzen kodierend diese Enzyme
GB9505479D0 (en) * 1995-03-17 1995-05-03 Danisco Enzyme
US6084155A (en) * 1995-06-06 2000-07-04 Novartis Ag Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes
GB9522589D0 (en) * 1995-11-03 1996-01-03 Innes John Centre Modified plants and plant products
GB9526613D0 (en) * 1995-12-28 1996-02-28 Scottish Crop Research Inst Sequence
JP2001501451A (ja) * 1996-03-05 2001-02-06 フリードリヒ ワイスハイメル マイツファブリク モルティング・プロセスによる、形質転換植物素材中に存在する貯蔵物質からの分解物質及び／又は転換物質の生成方法
DE19608918A1 (de) 1996-03-07 1997-09-11 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Mais codieren
DE19618125A1 (de) 1996-05-06 1997-11-13 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Kartoffel codieren
JP2000515381A (ja) * 1996-08-05 2000-11-21 モーヘン インターナショナル エヌ．ブイ． 麦芽種子を用いるアルコール飲料の製造のための改善されたプロセス
EP1574580A3 (de) * 1996-09-12 2009-07-01 Syngenta Participations AG Cellulolytische enzyme-exprimierende transgene Pflanzen
CN1230224A (zh) 1996-09-12 1999-09-29 诺瓦提斯公司 表达纤维素分解酶的转基因植物
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
US6818803B1 (en) 1997-06-26 2004-11-16 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
WO1999024588A1 (en) * 1997-11-07 1999-05-20 University Of Guelph cDNA CLONE OF ENDO-β-MANNANASE FROM PLANT TISSUES
DE19820608A1 (de) * 1998-05-08 1999-11-11 Hoechst Schering Agrevo Gmbh Nucleinsäuremoleküle codierend Enzyme aus Weizen, die an der Stärkesynthese beteiligt sind
DE19836097A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Nukleinsäuremoleküle kodierend für eine alpha-Glukosidase, Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
DE19836099A1 (de) * 1998-07-31 2000-02-03 Hoechst Schering Agrevo Gmbh Nukleinsäuremoleküle kodierend für eine ß-Amylase, Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke
AU1263100A (en) * 1998-11-12 2000-06-05 Novozymes A/S Transgenic plant expressing maltogenic alpha-amylase
US6940002B1 (en) 1998-11-12 2005-09-06 Novozymes A/S Transgenic plant expressing maltogenic alpha-amylase
ATE488585T1 (de) 2001-08-27 2010-12-15 Syngenta Participations Ag Selbstprozessierende pflanzen und pflanzenteile
JP2007527726A (ja) * 2004-03-08 2007-10-04 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 自己プロセシング植物および植物部分
MX2007010036A (es) 2005-03-16 2007-10-04 Syngenta Participations Ag Maiz evento 3272 y metodos para la deteccion del mismo.
BRPI0714876B1 (pt) 2006-08-04 2022-04-19 Verenium Corporation Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos
DK2069490T4 (en) 2006-12-21 2018-04-23 Syngenta Participations Ag Amylases and Glucoamylases, Nucleic Acids Encoding Them, and Methods of Preparation and Use thereof
US7915020B2 (en) 2007-06-01 2011-03-29 Syngenta Participations Ag Process for starch liquefaction and fermentation
US7727726B2 (en) 2007-06-01 2010-06-01 Syngenta Participations Ag Process for starch liquefaction and fermentation
US7914993B2 (en) 2007-06-01 2011-03-29 Syngenta Participations Ag. Process for starch liquefaction and fermentation
CN107105658A (zh) 2014-11-18 2017-08-29 拜耳作物科学股份公司 某些除草剂结合物在块根作物植物中的用途
BR112017023248A2 (pt) 2015-04-27 2018-08-07 Bayer Cropscience Ag uso de determinadas combinações de herbicida em plantas de raiz tuberosa.
BR112017023244A2 (pt) 2015-04-27 2018-08-07 Bayer Cropscience Ag uso de determinadas combinações de herbicida em plantas de raiz tuberosa.
CN104830898B (zh) * 2015-05-15 2018-06-05 湖南工程学院 一种提高人参根中人参皂苷Rg1含量的方法与应用
WO2017050635A1 (en) 2015-09-22 2017-03-30 Bayer Cropscience Aktiengesellschaft Use of certain active ingredient combinations in tuberous root crop plants
CN115646866A (zh) * 2018-06-30 2023-01-31 曹可瀚 一种建筑物清洗装置和清洗方法

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EP0406267A1 (de) * 1988-02-26 1991-01-09 Biosource Genetics Corporation Nichtnukleare chromosomale transformation
EP0428572A1 (de) * 1988-07-29 1991-05-29 Washington University School Of Medicine Herstellung von kommerziell wertvollen polypeptiden durch genetisch transformierte endospermgewebe
DD275704A1 (de) * 1988-09-23 1990-01-31 Akad Wissenschaften Ddr Verfahren zur herstellung von gerstenpflanzen
DK198089D0 (da) * 1989-04-24 1989-04-24 Danske Spritfabrikker Dna-materialer og anvendelse deraf
IL97645A (en) * 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
US5543576A (en) * 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
US5705375A (en) * 1990-09-13 1998-01-06 Mogen International, N.V. Transgenic plants having a modified carbohydrate content
DE19516807C2 (de) * 1995-05-08 1998-01-15 Siegfried Mache Vorrichtung zum Aufnehmen von kontinuierlich in einer Förderbahn einzeln hintereinander zugeführten Gegenständen und zum gruppenweisen Bereitstellen der Gegenstände

Also Published As

Publication number Publication date
ATE175238T1 (de) 1999-01-15
EP0479359B1 (de) 1998-12-30
IL99482A0 (en) 1992-08-18
CA2072656A1 (en) 1992-03-14
PT98967B (pt) 1999-02-26
US20060195940A1 (en) 2006-08-31
CA2072656C (en) 2005-06-14
IE913215A1 (en) 1992-02-25
NZ239789A (en) 1994-06-27
AU656920B2 (en) 1995-02-23
WO1992005259A1 (en) 1992-04-02
PT98967A (pt) 1992-07-31
EP0479359A1 (de) 1992-04-08
AU8651491A (en) 1992-04-15
DE69130698D1 (de) 1999-02-11
DK0479359T3 (da) 1999-08-30
JP3938402B2 (ja) 2007-06-27
JPH05502591A (ja) 1993-05-13
IL99482A (en) 2005-12-18

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Owner name: GIST-BROCADES N.V., DELFT, NL SYNGENTA MOGEN B.V.,