DE69013520D1 - Stabile integration von dna in bakterielle genome. - Google Patents

Stabile integration von dna in bakterielle genome.

Info

Publication number
DE69013520D1
DE69013520D1 DE69013520T DE69013520T DE69013520D1 DE 69013520 D1 DE69013520 D1 DE 69013520D1 DE 69013520 T DE69013520 T DE 69013520T DE 69013520 T DE69013520 T DE 69013520T DE 69013520 D1 DE69013520 D1 DE 69013520D1
Authority
DE
Germany
Prior art keywords
dna
stable integration
bacterial genome
replication
origin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69013520T
Other languages
English (en)
Other versions
DE69013520T2 (de
DE69013520T3 (de
Inventor
Steen Jorgensen
Per Jorgensen
Borge Diderichsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8148687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69013520(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of DE69013520D1 publication Critical patent/DE69013520D1/de
Publication of DE69013520T2 publication Critical patent/DE69013520T2/de
Application granted granted Critical
Publication of DE69013520T3 publication Critical patent/DE69013520T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69013520T 1989-12-18 1990-12-18 Stabile integration von dna in bakterielle genome. Expired - Lifetime DE69013520T3 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK639689A DK639689D0 (da) 1989-12-18 1989-12-18 Indfoering af dna i celler
DK6396/89 1989-12-18
PCT/DK1990/000332 WO1991009129A1 (en) 1989-12-18 1990-12-18 Stable integration of dna in bacterial genomes

Publications (3)

Publication Number Publication Date
DE69013520D1 true DE69013520D1 (de) 1994-11-24
DE69013520T2 DE69013520T2 (de) 1995-05-04
DE69013520T3 DE69013520T3 (de) 2004-09-23

Family

ID=8148687

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69013520T Expired - Lifetime DE69013520T3 (de) 1989-12-18 1990-12-18 Stabile integration von dna in bakterielle genome.

Country Status (11)

Country Link
EP (1) EP0506780B2 (de)
JP (1) JP3162707B2 (de)
AT (1) ATE113077T1 (de)
AU (1) AU643741B2 (de)
CA (1) CA2070676A1 (de)
DE (1) DE69013520T3 (de)
DK (2) DK639689D0 (de)
ES (1) ES2064077T5 (de)
FI (1) FI112502B (de)
NO (1) NO922381L (de)
WO (1) WO1991009129A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665711B1 (fr) * 1990-08-08 1993-08-13 Centre Nat Rech Scient Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue.
IL110299A0 (en) 1993-07-15 1994-10-21 Novo Nordisk Entotech Inc Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity
WO1996029418A1 (en) * 1995-03-22 1996-09-26 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2736066B1 (fr) * 1995-06-30 1998-11-20 Ajinomoto Kk Procede d'amplification d'un gene par transposon artificiel, bacterie coryneforme obtenue par ce procede et procede de production d'un acide amine a l'aide de cette bacterie
ATE293359T1 (de) 1997-12-22 2005-05-15 Novozymes As Kohlenhydratoxidase sowie verwendung derselben beim backen
ATE528394T1 (de) 1998-06-10 2011-10-15 Novozymes As Neuartige mannasen
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
AU2001265835A1 (en) 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
MXPA03011194A (es) 2001-06-06 2004-02-26 Novozymes As Endo-beta-1,4-glucanasa.
WO2003087149A2 (en) 2002-04-10 2003-10-23 Novozymes A/S Bacillus licheniformis mutant host cell
EP1995319B1 (de) 2002-04-10 2011-08-31 Novozymes A/S Mutierte Bacillus licheniformis Wirtszelle
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
CN1738899A (zh) 2002-12-11 2006-02-22 诺和酶股份有限公司 洗涤剂组合物
US8268583B2 (en) 2003-12-10 2012-09-18 Novozymes A/S Cell with improved secretion mediated by MrgA protein or homologue
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
CN101595214B (zh) 2006-11-29 2013-06-12 诺维信股份有限公司 改进向细菌细胞中导入dna的方法
CN101611145B (zh) 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
WO2011084695A1 (en) 2009-12-21 2011-07-14 Novozymes, Inc. Methods for producing heterologous polypeptides in thiol-disulfide oxidoreductase-deficient bacterial mutant cells
EP3532619A1 (de) 2016-10-25 2019-09-04 Novozymes A/S Flp-vermittelte genomische integration
EP3655528A1 (de) 2017-07-21 2020-05-27 Basf Se Verfahren zur transformation von bakterienzellen
FI3655422T3 (fi) 2017-07-21 2023-06-13 Basf Se Heterologisen ilmentymisen promoottori
US20200318086A1 (en) 2017-11-10 2020-10-08 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
WO2020156903A1 (en) 2019-01-30 2020-08-06 Novozymes A/S Cognate foldase co-expression
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
CN113785056A (zh) 2019-03-08 2021-12-10 诺维信公司 改善蛋白酶表达的手段和方法
CN113939588A (zh) 2019-05-15 2022-01-14 诺维信公司 温度敏感性的rna指导的内切核酸酶
EP3990629A1 (de) 2019-06-25 2022-05-04 Novozymes A/S Gegenauswahl durch hemmung von bedingt essenziellen genen
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
BR112023001384A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para cultivar uma célula hospedeira de bacillus, e, cultura de célula hospedeira de bacillus
CN116323960A (zh) 2020-07-28 2023-06-23 巴斯夫欧洲公司 使用部分收获的芽孢杆菌工业发酵工艺
MX2023001273A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion.
EP4225779A2 (de) 2020-10-07 2023-08-16 Basf Se Bacilluszelle mit reduzierter lipase- und/oder esteraseseitenaktivität
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
CN117730090A (zh) 2021-07-13 2024-03-19 诺维信公司 重组角质酶表达
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
WO2024028338A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631257A (en) * 1978-12-26 1986-12-23 Cetus Corporation Stable high copy number plasmids
US4401761A (en) * 1981-01-26 1983-08-30 The Upjohn Company Process for stabilizing plasmids by deletion of DNA

Also Published As

Publication number Publication date
EP0506780B1 (de) 1994-10-19
JPH05502162A (ja) 1993-04-22
CA2070676A1 (en) 1991-06-19
AU643741B2 (en) 1993-11-25
DK0506780T3 (da) 1995-04-18
NO922381L (no) 1992-08-17
AU7031291A (en) 1991-07-18
EP0506780B2 (de) 2004-01-02
WO1991009129A1 (en) 1991-06-27
EP0506780A1 (de) 1992-10-07
DE69013520T2 (de) 1995-05-04
FI922812A (fi) 1992-06-17
JP3162707B2 (ja) 2001-05-08
DK639689D0 (da) 1989-12-18
ES2064077T5 (es) 2004-09-01
ES2064077T3 (es) 1995-01-16
DK0506780T4 (da) 2004-04-26
FI112502B (fi) 2003-12-15
ATE113077T1 (de) 1994-11-15
DE69013520T3 (de) 2004-09-23
FI922812A0 (fi) 1992-06-17
NO922381D0 (no) 1992-06-17

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: NOVOZYMES A/S, BAGSVAERD, DK

8366 Restricted maintained after opposition proceedings