ATE113077T1 - Stabile integration von dna in bakterielle genome. - Google Patents
Stabile integration von dna in bakterielle genome.Info
- Publication number
- ATE113077T1 ATE113077T1 AT91901587T AT91901587T ATE113077T1 AT E113077 T1 ATE113077 T1 AT E113077T1 AT 91901587 T AT91901587 T AT 91901587T AT 91901587 T AT91901587 T AT 91901587T AT E113077 T1 ATE113077 T1 AT E113077T1
- Authority
- AT
- Austria
- Prior art keywords
- dna
- stable integration
- replication
- bacterial genomes
- origin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK639689A DK639689D0 (da) | 1989-12-18 | 1989-12-18 | Indfoering af dna i celler |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE113077T1 true ATE113077T1 (de) | 1994-11-15 |
Family
ID=8148687
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT91901587T ATE113077T1 (de) | 1989-12-18 | 1990-12-18 | Stabile integration von dna in bakterielle genome. |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0506780B2 (de) |
JP (1) | JP3162707B2 (de) |
AT (1) | ATE113077T1 (de) |
AU (1) | AU643741B2 (de) |
CA (1) | CA2070676A1 (de) |
DE (1) | DE69013520T3 (de) |
DK (2) | DK639689D0 (de) |
ES (1) | ES2064077T5 (de) |
FI (1) | FI112502B (de) |
NO (1) | NO922381L (de) |
WO (1) | WO1991009129A1 (de) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2665711B1 (fr) * | 1990-08-08 | 1993-08-13 | Centre Nat Rech Scient | Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue. |
IL110299A0 (en) | 1993-07-15 | 1994-10-21 | Novo Nordisk Entotech Inc | Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity |
EP0817856A1 (de) * | 1995-03-22 | 1998-01-14 | Novo Nordisk A/S | Einfuhrung von dna in bacillus subtilis durch konjugation |
EP0756007A3 (de) * | 1995-06-30 | 1997-10-29 | Ajinomoto Kk | Genvermerungsverfahren mit kunstlichen Transposon |
AU753578B2 (en) | 1997-12-22 | 2002-10-24 | Novozymes A/S | Carbohydrate oxidase and use thereof in baking |
KR20010052736A (ko) | 1998-06-10 | 2001-06-25 | 피아 스타르 | 신규한 만난나제 |
JP3958884B2 (ja) | 1998-09-11 | 2007-08-15 | 株式会社林原生物化学研究所 | 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法 |
ATE332968T1 (de) | 1998-10-26 | 2006-08-15 | Novozymes As | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen |
EP1297170B1 (de) | 2000-06-23 | 2016-03-02 | Novozymes A/S | Verfahren zur stabilen chromosomalen integration von mehreren kopien von genen |
CN101864406B (zh) | 2001-06-06 | 2016-03-30 | 诺维信公司 | 内切-β-1,4-葡聚糖酶 |
US7521204B2 (en) | 2002-04-10 | 2009-04-21 | Novozymes A/S | Bacillus host cell |
EP1497431A2 (de) | 2002-04-10 | 2005-01-19 | Novozymes A/S | Verbesserte bacillus-wirtszelle |
AU2003223928A1 (en) * | 2002-05-07 | 2003-11-11 | Novozymes A/S | Homologous recombination into bacterium for the generation of polynucleotide libraries |
CN1738899A (zh) | 2002-12-11 | 2006-02-22 | 诺和酶股份有限公司 | 洗涤剂组合物 |
DK1694846T3 (en) | 2003-12-10 | 2014-11-17 | Novozymes As | Cell with enhanced secretion mediated by MrgA protein or a homolog |
US20080044853A1 (en) | 2004-06-21 | 2008-02-21 | Novozymes A/S | Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation |
DK2089516T3 (da) | 2006-11-29 | 2012-02-20 | Novozymes Inc | Fremgangsmåder til at forbedre indføringen af DNA i bakterielle celler |
US8268586B2 (en) | 2006-12-21 | 2012-09-18 | Novozymes, Inc. | Modified messenger RNA stabilizing sequences for expressing genes in bacterial cells |
CN102762723A (zh) | 2009-12-21 | 2012-10-31 | 诺维信股份有限公司 | 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法 |
CN109804072A (zh) | 2016-10-25 | 2019-05-24 | 诺维信公司 | 地衣芽孢杆菌中flp介导的基因组整合 |
FI3655422T3 (fi) | 2017-07-21 | 2023-06-13 | Basf Se | Heterologisen ilmentymisen promoottori |
CN110892066A (zh) | 2017-07-21 | 2020-03-17 | 巴斯夫欧洲公司 | 转化细菌细胞的方法 |
WO2019092042A1 (en) | 2017-11-10 | 2019-05-16 | Novozymes A/S | Temperature-sensitive cas9 protein |
US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
EP3918086A1 (de) | 2019-01-30 | 2021-12-08 | Novozymes A/S | Verwandte foldase-koexpression |
WO2020169564A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and trace element feed |
MX2021010110A (es) | 2019-02-20 | 2021-09-21 | Basf Se | Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio. |
CN113785056A (zh) | 2019-03-08 | 2021-12-10 | 诺维信公司 | 改善蛋白酶表达的手段和方法 |
US20220275354A1 (en) | 2019-05-15 | 2022-09-01 | Novozymes A/S | TEMPERATURE-SENSITIVE RNA- Guided Endonuclease |
EP3990629A1 (de) | 2019-06-25 | 2022-05-04 | Novozymes A/S | Gegenauswahl durch hemmung von bedingt essenziellen genen |
US20220340865A1 (en) | 2019-07-02 | 2022-10-27 | Basf Se | Method for preparing a fermentation medium |
CN116323960A (zh) | 2020-07-28 | 2023-06-23 | 巴斯夫欧洲公司 | 使用部分收获的芽孢杆菌工业发酵工艺 |
MX2023001273A (es) | 2020-07-28 | 2023-03-03 | Basf Se | Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion. |
EP4189103A1 (de) | 2020-07-28 | 2023-06-07 | Basf Se | Industrielles fermentationsverfahren für bacillus unter verwendung von temperaturverschiebung |
WO2022074056A2 (en) | 2020-10-07 | 2022-04-14 | Basf Se | Bacillus cell with reduced lipase and/or esterase side activities |
BR112023027009A2 (pt) | 2021-06-24 | 2024-03-12 | Basf Se | Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse |
WO2022269084A1 (en) | 2021-06-24 | 2022-12-29 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
EP4370534A1 (de) | 2021-07-13 | 2024-05-22 | Novozymes A/S | Rekombinante cutinase-expression |
WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
WO2024028340A1 (en) | 2022-08-02 | 2024-02-08 | Basf Se | Stabilized protein production process using bacillus host cells with salt feed |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4631257A (en) * | 1978-12-26 | 1986-12-23 | Cetus Corporation | Stable high copy number plasmids |
US4401761A (en) * | 1981-01-26 | 1983-08-30 | The Upjohn Company | Process for stabilizing plasmids by deletion of DNA |
-
1989
- 1989-12-18 DK DK639689A patent/DK639689D0/da not_active Application Discontinuation
-
1990
- 1990-12-18 EP EP91901587A patent/EP0506780B2/de not_active Expired - Lifetime
- 1990-12-18 WO PCT/DK1990/000332 patent/WO1991009129A1/en active IP Right Grant
- 1990-12-18 AT AT91901587T patent/ATE113077T1/de not_active IP Right Cessation
- 1990-12-18 CA CA002070676A patent/CA2070676A1/en not_active Abandoned
- 1990-12-18 DE DE69013520T patent/DE69013520T3/de not_active Expired - Lifetime
- 1990-12-18 ES ES91901587T patent/ES2064077T5/es not_active Expired - Lifetime
- 1990-12-18 AU AU70312/91A patent/AU643741B2/en not_active Expired
- 1990-12-18 JP JP50181391A patent/JP3162707B2/ja not_active Expired - Lifetime
- 1990-12-18 DK DK91901587T patent/DK0506780T4/da active
-
1992
- 1992-06-17 NO NO92922381A patent/NO922381L/no unknown
- 1992-06-17 FI FI922812A patent/FI112502B/fi active
Also Published As
Publication number | Publication date |
---|---|
CA2070676A1 (en) | 1991-06-19 |
AU7031291A (en) | 1991-07-18 |
WO1991009129A1 (en) | 1991-06-27 |
NO922381L (no) | 1992-08-17 |
NO922381D0 (no) | 1992-06-17 |
JPH05502162A (ja) | 1993-04-22 |
FI922812A (fi) | 1992-06-17 |
DE69013520T2 (de) | 1995-05-04 |
EP0506780A1 (de) | 1992-10-07 |
FI112502B (fi) | 2003-12-15 |
DK0506780T4 (da) | 2004-04-26 |
DE69013520D1 (de) | 1994-11-24 |
EP0506780B2 (de) | 2004-01-02 |
FI922812A0 (fi) | 1992-06-17 |
AU643741B2 (en) | 1993-11-25 |
JP3162707B2 (ja) | 2001-05-08 |
DK0506780T3 (da) | 1995-04-18 |
ES2064077T3 (es) | 1995-01-16 |
ES2064077T5 (es) | 2004-09-01 |
DE69013520T3 (de) | 2004-09-23 |
EP0506780B1 (de) | 1994-10-19 |
DK639689D0 (da) | 1989-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE113077T1 (de) | Stabile integration von dna in bakterielle genome. | |
ES2053758T3 (es) | Una nueva molecula polipeptidica. | |
ES8500322A1 (es) | Un metodo de modificar un virus para conferirle una nueva funcion biologica. | |
DK219087D0 (da) | Vektorsystem til brug ved transformation af trichoderma, transformerede trichodermastammer og fremgangsmaade til fremstilling af proteiner heri | |
ATE27000T1 (de) | Expression von polypeptiden in hefen. | |
ATE87811T1 (de) | Satz zur herstellung und abscheidung von endothelzellen. | |
AU6329694A (en) | Recombinant expression vectors and purification methods for thermus thermophilus DNA polymerase | |
DE3864585D1 (de) | Dns-molekule, codierend fuer non-a-non-b-hepatitis-antigene und ihre verwendung zur herstellung dieser antigene. | |
DK1724354T3 (da) | Cirkulær DNA-molekyle med en afhængig replikationsoprindelse, fremgangsmåde til fremstilling heraf og anvendelse heraf i genterapi | |
DE69334211D1 (de) | Multidrug resistenz gen | |
Kuroiwa et al. | A method of isolation of mitochondrial nucleoid of Physarum polycephalum and evidence for the presence of a basic protein | |
DK388789A (da) | Hybridderivat af humant protein d, dets fremstilling og anvendelse samt dna, som koder derfor, og genstruktur og vaertscelle indeholdende denne dna | |
ES2054612T3 (es) | Secuencias de adn que codifican para proteinas con actividad biologica de los inhibidores husi tipo-i y procedimientos tecnologicos geneticos para la preparacion de estas proteinas. | |
ES8706827A1 (es) | Procedimiento para preparar polipeptidos. | |
DE3881023D1 (de) | Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten. | |
DE69031042D1 (de) | Fermentationsprozess zur herstellung von pyrimidin | |
DK610488D0 (da) | Antikoagulatorisk protein pp4-x, dets fremstilling og anvendelse | |
DK135588D0 (da) | Inaktivering af hiv i proteinholdige oploesninger ved hjaelp af phenoler | |
DK408488D0 (da) | Fremgangsmaade til bestemmelse af tilstedevaerelse af et gen derkoder for syntese af beta-lactamase i en mikroorganisme, og dna-prober til brug ved fremgangsmaaden | |
IL83435A (en) | Dna construct for expression of genes in yeast, an expression vector containing it and yeast transformed with said expression vector | |
DK233587A (da) | Dna-fragment og dets anvendelse ved fremstilling af streptokinase | |
DE3751114D1 (de) | Dmd-sonden. | |
NO160605C (no) | Fremgangsmaate for bearbeiding av avfallsstoffer ved fremstilling av karbonelektroder, og adsorbator for saadan bearbeiding. | |
DK0635061T3 (da) | Fremgangsmåde til begrænsning af genetisk manipulerede organismers overlevelse i deres miljø | |
Eagle | Chemical aspects of discharges. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification |
Ref document number: 0506780 Country of ref document: EP |
|
EELA | Cancelled due to lapse of time |