ATE113077T1 - Stabile integration von dna in bakterielle genome. - Google Patents

Stabile integration von dna in bakterielle genome.

Info

Publication number
ATE113077T1
ATE113077T1 AT91901587T AT91901587T ATE113077T1 AT E113077 T1 ATE113077 T1 AT E113077T1 AT 91901587 T AT91901587 T AT 91901587T AT 91901587 T AT91901587 T AT 91901587T AT E113077 T1 ATE113077 T1 AT E113077T1
Authority
AT
Austria
Prior art keywords
dna
stable integration
replication
bacterial genomes
origin
Prior art date
Application number
AT91901587T
Other languages
English (en)
Inventor
Steen Troels Jorgensen
Per Lina Jorgensen
Borge Krag Diderichsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8148687&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE113077(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE113077T1 publication Critical patent/ATE113077T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT91901587T 1989-12-18 1990-12-18 Stabile integration von dna in bakterielle genome. ATE113077T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK639689A DK639689D0 (da) 1989-12-18 1989-12-18 Indfoering af dna i celler

Publications (1)

Publication Number Publication Date
ATE113077T1 true ATE113077T1 (de) 1994-11-15

Family

ID=8148687

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91901587T ATE113077T1 (de) 1989-12-18 1990-12-18 Stabile integration von dna in bakterielle genome.

Country Status (11)

Country Link
EP (1) EP0506780B2 (de)
JP (1) JP3162707B2 (de)
AT (1) ATE113077T1 (de)
AU (1) AU643741B2 (de)
CA (1) CA2070676A1 (de)
DE (1) DE69013520T3 (de)
DK (2) DK639689D0 (de)
ES (1) ES2064077T5 (de)
FI (1) FI112502B (de)
NO (1) NO922381L (de)
WO (1) WO1991009129A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2665711B1 (fr) * 1990-08-08 1993-08-13 Centre Nat Rech Scient Integron de corynebacterie, procede de transformation d'une corynebacterie par ledit integron et corynebacterie obtenue.
IL110299A0 (en) 1993-07-15 1994-10-21 Novo Nordisk Entotech Inc Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity
WO1996029418A1 (en) * 1995-03-22 1996-09-26 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
EP0756007A3 (de) * 1995-06-30 1997-10-29 Ajinomoto Kk Genvermerungsverfahren mit kunstlichen Transposon
JP4417550B2 (ja) 1997-12-22 2010-02-17 ノボザイムス アクティーゼルスカブ 糖質酸化酵素およびベーキングにおけるその使用
EP1086211B1 (de) 1998-06-10 2011-10-12 Novozymes A/S Neuartige mannasen
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
WO2002000907A1 (en) 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
MXPA03011194A (es) 2001-06-06 2004-02-26 Novozymes As Endo-beta-1,4-glucanasa.
EP1497429B1 (de) 2002-04-10 2008-07-02 Novozymes A/S Verbesserte bacillus-wirtszelle
US20050176097A1 (en) 2002-04-10 2005-08-11 Andersen Jens T. Bacillus host cell
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
EP2311941B1 (de) 2002-12-11 2014-03-19 Novozymes A/S Endoglukanase-enthaltendes Waschmittel
EP1694846B1 (de) 2003-12-10 2014-10-01 Novozymes A/S Zelle mit verbesserter, vom protein mrga oder einem homolog vermittelter sekretion
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
ATE533838T1 (de) 2006-11-29 2011-12-15 Novozymes Inc Verfahren zur verbesserten einführung von dna in bakterienzellen
WO2008140615A2 (en) 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
EP3532619A1 (de) 2016-10-25 2019-09-04 Novozymes A/S Flp-vermittelte genomische integration
PL3655422T3 (pl) 2017-07-21 2023-07-24 Basf Se Promotor ekspresji heterologicznej
EP3655528A1 (de) 2017-07-21 2020-05-27 Basf Se Verfahren zur transformation von bakterienzellen
WO2019092042A1 (en) 2017-11-10 2019-05-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
US20220112478A1 (en) 2019-01-30 2022-04-14 Novozymes A/S Cognate Foldase Co-Expression
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
US20220170003A1 (en) 2019-03-08 2022-06-02 Novozymes A/S Means And Methods For Improving Protease Expression
EP3969576A1 (de) 2019-05-15 2022-03-23 Novozymes A/S Temperaturempfindliche rna-geführte endonuklease
WO2020260061A1 (en) 2019-06-25 2020-12-30 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
WO2021001297A1 (en) 2019-07-02 2021-01-07 Basf Se Method for preparing a fermentation medium
WO2022023372A1 (en) 2020-07-28 2022-02-03 Basf Se Industrial fermentation process for bacillus using feed rate shift
MX2023001270A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de recoleccion parcial.
EP4189103A1 (de) 2020-07-28 2023-06-07 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung von temperaturverschiebung
EP4225779A2 (de) 2020-10-07 2023-08-16 Basf Se Bacilluszelle mit reduzierter lipase- und/oder esteraseseitenaktivität
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
BR112023027009A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus licheniformis, e, métodos para produzir um composto de interesse e para aumentar a pureza de um composto de interesse
EP4370534A1 (de) 2021-07-13 2024-05-22 Novozymes A/S Rekombinante cutinase-expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression
WO2024028338A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4631257A (en) * 1978-12-26 1986-12-23 Cetus Corporation Stable high copy number plasmids
US4401761A (en) * 1981-01-26 1983-08-30 The Upjohn Company Process for stabilizing plasmids by deletion of DNA

Also Published As

Publication number Publication date
DE69013520T2 (de) 1995-05-04
DK0506780T4 (da) 2004-04-26
NO922381D0 (no) 1992-06-17
DK639689D0 (da) 1989-12-18
NO922381L (no) 1992-08-17
ES2064077T3 (es) 1995-01-16
EP0506780B1 (de) 1994-10-19
EP0506780A1 (de) 1992-10-07
AU643741B2 (en) 1993-11-25
ES2064077T5 (es) 2004-09-01
FI112502B (fi) 2003-12-15
CA2070676A1 (en) 1991-06-19
WO1991009129A1 (en) 1991-06-27
DE69013520T3 (de) 2004-09-23
EP0506780B2 (de) 2004-01-02
DE69013520D1 (de) 1994-11-24
DK0506780T3 (da) 1995-04-18
JP3162707B2 (ja) 2001-05-08
FI922812A (fi) 1992-06-17
JPH05502162A (ja) 1993-04-22
AU7031291A (en) 1991-07-18
FI922812A0 (fi) 1992-06-17

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