DE68926038T2 - Gereinigtes thermostabiles enzym - Google Patents
Gereinigtes thermostabiles enzymInfo
- Publication number
- DE68926038T2 DE68926038T2 DE68926038T DE68926038T DE68926038T2 DE 68926038 T2 DE68926038 T2 DE 68926038T2 DE 68926038 T DE68926038 T DE 68926038T DE 68926038 T DE68926038 T DE 68926038T DE 68926038 T2 DE68926038 T2 DE 68926038T2
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- recombinant
- thermostable
- cleaned
- thermostable enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14344188A | 1988-01-12 | 1988-01-12 | |
US143441 | 1988-01-12 | ||
PCT/US1989/000127 WO1989006691A2 (en) | 1988-01-12 | 1989-01-12 | Purified thermostable enzyme |
Publications (3)
Publication Number | Publication Date |
---|---|
DE68926038D1 DE68926038D1 (de) | 1996-04-25 |
DE68926038T2 true DE68926038T2 (de) | 1996-10-17 |
DE68926038T3 DE68926038T3 (de) | 2007-02-01 |
Family
ID=22504093
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE68926038T Expired - Lifetime DE68926038T3 (de) | 1988-01-12 | 1989-01-12 | Gereinigtes thermostabiles enzym |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP0395736B2 (de) |
JP (1) | JP2511548B2 (de) |
AT (1) | ATE135741T1 (de) |
AU (1) | AU3062989A (de) |
CA (1) | CA1341143C (de) |
DE (1) | DE68926038T3 (de) |
HK (1) | HK166096A (de) |
IE (1) | IE72180B1 (de) |
IL (1) | IL88923A (de) |
SG (1) | SG46657A1 (de) |
WO (1) | WO1989006691A2 (de) |
ZA (1) | ZA89262B (de) |
Families Citing this family (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5079352A (en) * | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
US6127155A (en) * | 1986-08-22 | 2000-10-03 | Roche Molecular Systems, Inc. | Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents |
US5405774A (en) * | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
US5455170A (en) * | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
US5618711A (en) * | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
AU627809B2 (en) * | 1988-12-16 | 1992-09-03 | Abbott Laboratories | Isolating thermostable enzymes from engineered mesophiles |
US5270179A (en) * | 1989-08-10 | 1993-12-14 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity |
US5541099A (en) * | 1989-08-10 | 1996-07-30 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity |
US5047342A (en) * | 1989-08-10 | 1991-09-10 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase |
ES2121777T3 (es) * | 1989-12-22 | 1998-12-16 | Hoffmann La Roche | Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus. |
EP0894860B1 (de) * | 1990-09-28 | 2012-12-12 | F. Hoffmann-La Roche AG | Thermostabile DNA Polymerasen und deren Deletionsmutanten |
DK0550696T3 (da) * | 1990-09-28 | 2001-03-19 | Hoffmann La Roche | Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus |
AU8906091A (en) * | 1990-10-05 | 1992-04-28 | Wayne M. Barnes | Thermostable dna polymerase |
US6083686A (en) * | 1990-10-26 | 2000-07-04 | Johnson & Johnson Clinical Diagnostic Systems, Inc. | Increased production of Thermus aquaticus DNA polymerase in E. coli |
EP1233061A3 (de) * | 1991-06-03 | 2002-09-04 | Takara Shuzo Co., Ltd. | Verfahren zum Klonieren eines Pol-I-Typ DNS-Polymerase-Gens |
US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
US6410277B1 (en) | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US7465539B1 (en) | 1993-02-19 | 2008-12-16 | Takara Bio, Inc. | DNA polymerases with enhanced length of primer extension |
US5474920A (en) * | 1993-11-23 | 1995-12-12 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Modified thermo-resistant DNA polymerases |
US7312052B1 (en) | 1993-12-08 | 2007-12-25 | Stratagene California | Polymerase compositions and uses thereof |
US5622822A (en) | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
US5705366A (en) | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
EP2264045B1 (de) * | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
DE69838724T2 (de) | 1997-03-21 | 2008-10-30 | Stratagene California, La Jolla | Polymerase-verbessernder faktor (pef)-enthaltende extrakte, pef proteinkomplexe, isoliertes pef protein und verfahren zur reinigung und identifizierung |
ES2228520T3 (es) | 1999-05-04 | 2005-04-16 | Ortho-Clinical Diagnostics, Inc. | Capatura rapida y eficaz de adn de una muestra sin usar reactivo de lisis celular. |
US6632645B1 (en) | 2000-03-02 | 2003-10-14 | Promega Corporation | Thermophilic DNA polymerases from Thermoactinomyces vulgaris |
US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
US7262006B1 (en) | 2000-05-01 | 2007-08-28 | Ortho-Clinical Diagnostics, Inc. | Rapid and efficient capture of DNA from sample without using cell lysing reagent |
US6617136B2 (en) | 2001-04-24 | 2003-09-09 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
JP4568571B2 (ja) * | 2004-10-06 | 2010-10-27 | ユニバーサル・バイオ・リサーチ株式会社 | 熱安定性又は熱活性dnaポリメラーゼ及びそれをコードするdna |
JP4699008B2 (ja) * | 2004-11-02 | 2011-06-08 | 旭化成株式会社 | 酵素反応試薬 |
US7972828B2 (en) * | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
EP2113574A1 (de) | 2008-04-28 | 2009-11-04 | Biotype AG | Stoffe und Verfahren für Profilierungstest auf DNA-Basis |
US20110250598A1 (en) * | 2010-04-12 | 2011-10-13 | Ulrike Fischer | Detergent free polymerases |
US9845489B2 (en) | 2010-07-26 | 2017-12-19 | Biomatrica, Inc. | Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
WO2012018638A2 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
WO2012146260A1 (de) | 2011-04-23 | 2012-11-01 | Biolytix Ag | Herstellung und verwendung von proteinen in der molekularbiologie |
US20140113302A1 (en) | 2011-05-06 | 2014-04-24 | Qiagen Gmbh | Methods for sequencing, amplification and detection of nucleic acids comprising internally labelled primer |
WO2014100755A2 (en) | 2012-12-20 | 2014-06-26 | Biomatrica, Inc. | Formulations and methods for stabilizing pcr reagents |
ES2786373T3 (es) | 2014-06-10 | 2020-10-09 | Biomatrica Inc | Estabilización de trombocitos a temperaturas ambiente |
ES2946184T3 (es) | 2015-12-08 | 2023-07-13 | Biomatrica Inc | Reducción de la velocidad de eritrosedimentación |
CN110218777B (zh) * | 2019-06-14 | 2023-05-16 | 苏州天合优才生物科技有限公司 | Pcr预混液 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0822261B2 (ja) * | 1986-03-07 | 1996-03-06 | 株式会社日立製作所 | 電気掃除機 |
CA1284931C (en) * | 1986-03-13 | 1991-06-18 | Henry A. Erlich | Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids |
CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
-
1989
- 1989-01-10 IL IL8892389A patent/IL88923A/en not_active IP Right Cessation
- 1989-01-11 CA CA000587978A patent/CA1341143C/en not_active Expired - Lifetime
- 1989-01-11 IE IE6889A patent/IE72180B1/en not_active IP Right Cessation
- 1989-01-12 JP JP1502251A patent/JP2511548B2/ja not_active Expired - Lifetime
- 1989-01-12 EP EP89902422A patent/EP0395736B2/de not_active Expired - Lifetime
- 1989-01-12 SG SG1996007388A patent/SG46657A1/en unknown
- 1989-01-12 AU AU30629/89A patent/AU3062989A/en not_active Abandoned
- 1989-01-12 ZA ZA89262A patent/ZA89262B/xx unknown
- 1989-01-12 AT AT89902422T patent/ATE135741T1/de not_active IP Right Cessation
- 1989-01-12 WO PCT/US1989/000127 patent/WO1989006691A2/en active IP Right Grant
- 1989-01-12 DE DE68926038T patent/DE68926038T3/de not_active Expired - Lifetime
-
1996
- 1996-09-05 HK HK166096A patent/HK166096A/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE68926038T3 (de) | 2007-02-01 |
SG46657A1 (en) | 1998-02-20 |
WO1989006691A3 (en) | 1989-11-16 |
IL88923A (en) | 1995-07-31 |
IE890068L (en) | 1989-07-12 |
EP0395736B2 (de) | 2006-08-23 |
IE72180B1 (en) | 1997-03-26 |
DE68926038D1 (de) | 1996-04-25 |
JPH03502165A (ja) | 1991-05-23 |
IL88923A0 (en) | 1989-08-15 |
EP0395736A1 (de) | 1990-11-07 |
CA1341143C (en) | 2000-11-21 |
EP0395736B1 (de) | 1996-03-20 |
HK166096A (en) | 1996-09-13 |
ATE135741T1 (de) | 1996-04-15 |
WO1989006691A2 (en) | 1989-07-27 |
JP2511548B2 (ja) | 1996-06-26 |
ZA89262B (en) | 1990-09-26 |
AU3062989A (en) | 1989-08-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8363 | Opposition against the patent | ||
8366 | Restricted maintained after opposition proceedings |