DE60314395T2 - Verfahren zum messen von protein kinase und phosphatase aktivität - Google Patents

Verfahren zum messen von protein kinase und phosphatase aktivität Download PDF

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Publication number
DE60314395T2
DE60314395T2 DE60314395T DE60314395T DE60314395T2 DE 60314395 T2 DE60314395 T2 DE 60314395T2 DE 60314395 T DE60314395 T DE 60314395T DE 60314395 T DE60314395 T DE 60314395T DE 60314395 T2 DE60314395 T2 DE 60314395T2
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DE
Germany
Prior art keywords
substrate
enzyme
phosphorylated
activity
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60314395T
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German (de)
English (en)
Other versions
DE60314395D1 (de
Inventor
John Gerard Amers Whateley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
GE Healthcare UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare UK Ltd filed Critical GE Healthcare UK Ltd
Publication of DE60314395D1 publication Critical patent/DE60314395D1/de
Application granted granted Critical
Publication of DE60314395T2 publication Critical patent/DE60314395T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60314395T 2002-04-19 2003-04-17 Verfahren zum messen von protein kinase und phosphatase aktivität Expired - Lifetime DE60314395T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0208987.8A GB0208987D0 (en) 2002-04-19 2002-04-19 Methods for measuring protein kinase and phosphatase activity
GB0208987 2002-04-19
PCT/GB2003/001683 WO2003089665A1 (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Publications (2)

Publication Number Publication Date
DE60314395D1 DE60314395D1 (de) 2007-07-26
DE60314395T2 true DE60314395T2 (de) 2008-02-21

Family

ID=9935150

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60314395T Expired - Lifetime DE60314395T2 (de) 2002-04-19 2003-04-17 Verfahren zum messen von protein kinase und phosphatase aktivität

Country Status (10)

Country Link
US (1) US7125682B2 (enExample)
EP (1) EP1497453B1 (enExample)
JP (1) JP4615867B2 (enExample)
AT (1) ATE364715T1 (enExample)
AU (1) AU2003227872A1 (enExample)
CA (1) CA2484397C (enExample)
DE (1) DE60314395T2 (enExample)
GB (2) GB0208987D0 (enExample)
IL (1) IL164543A0 (enExample)
WO (1) WO2003089665A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008011578A1 (de) * 2008-02-28 2009-09-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Photolumineszenz-Sensor

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090118139A1 (en) * 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
EP1467211B1 (de) * 2003-04-10 2008-04-30 Tecan Trading AG Verfahren zum Messen von an biologische Moleküle gebundenen, chemischen Gruppen
WO2005043137A1 (de) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer
WO2006132657A2 (en) * 2004-09-22 2006-12-14 Applera Corporation Enzyme assay with nanowire sensor
WO2007049057A2 (en) * 2005-10-28 2007-05-03 Iti Scotland Limited Novel fluorescent dyes and uses thereof
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
FI20070508A0 (fi) * 2007-06-27 2007-06-27 Turun Yliopisto Peptidisubstraatti ja sen käyttö
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032381A (en) * 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
DE69739898D1 (de) * 1996-07-16 2010-07-15 Univ California Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten
JP3815051B2 (ja) 1997-04-30 2006-08-30 和光純薬工業株式会社 新規なペプチド誘導体
ATE233325T1 (de) * 1997-12-05 2003-03-15 Upjohn Co Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
JP2001019700A (ja) 1999-07-07 2001-01-23 Mitsubishi Chemicals Corp 蛍光標識ペプチド
ATE291096T1 (de) * 1999-07-30 2005-04-15 Bayer Ag Chemilumineszenz-substrate von hydrolytischen enzymen wie phosphatasen
EP1264897A3 (en) 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetic sensor peptide for kinase or phosphatase assays

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008011578A1 (de) * 2008-02-28 2009-09-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Photolumineszenz-Sensor

Also Published As

Publication number Publication date
GB2387905A (en) 2003-10-29
GB0208987D0 (en) 2002-05-29
GB2387905B (en) 2005-01-12
JP4615867B2 (ja) 2011-01-19
GB0308903D0 (en) 2003-05-21
JP2005523024A (ja) 2005-08-04
CA2484397A1 (en) 2003-10-30
ATE364715T1 (de) 2007-07-15
AU2003227872A1 (en) 2003-11-03
US20030228646A1 (en) 2003-12-11
CA2484397C (en) 2014-06-17
EP1497453A1 (en) 2005-01-19
US7125682B2 (en) 2006-10-24
WO2003089665A1 (en) 2003-10-30
DE60314395D1 (de) 2007-07-26
IL164543A0 (en) 2005-12-18
EP1497453B1 (en) 2007-06-13
WO2003089665A8 (en) 2004-11-18

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