AU2003227872A1 - Methods for measuring protein kinase and phosphatase activity - Google Patents

Methods for measuring protein kinase and phosphatase activity Download PDF

Info

Publication number
AU2003227872A1
AU2003227872A1 AU2003227872A AU2003227872A AU2003227872A1 AU 2003227872 A1 AU2003227872 A1 AU 2003227872A1 AU 2003227872 A AU2003227872 A AU 2003227872A AU 2003227872 A AU2003227872 A AU 2003227872A AU 2003227872 A1 AU2003227872 A1 AU 2003227872A1
Authority
AU
Australia
Prior art keywords
substrate
enzyme
phosphorylated
labelled
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003227872A
Other languages
English (en)
Inventor
John Gerard Whatley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
GE Healthcare UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare UK Ltd filed Critical GE Healthcare UK Ltd
Publication of AU2003227872A1 publication Critical patent/AU2003227872A1/en
Assigned to GE HEALTHCARE UK LIMITED reassignment GE HEALTHCARE UK LIMITED Request for Assignment Assignors: Amersham Bio-Sciences UK Limited
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2003227872A 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity Abandoned AU2003227872A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0208987.8A GB0208987D0 (en) 2002-04-19 2002-04-19 Methods for measuring protein kinase and phosphatase activity
GB0208987.8 2002-04-19
PCT/GB2003/001683 WO2003089665A1 (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Publications (1)

Publication Number Publication Date
AU2003227872A1 true AU2003227872A1 (en) 2003-11-03

Family

ID=9935150

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003227872A Abandoned AU2003227872A1 (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Country Status (10)

Country Link
US (1) US7125682B2 (enExample)
EP (1) EP1497453B1 (enExample)
JP (1) JP4615867B2 (enExample)
AT (1) ATE364715T1 (enExample)
AU (1) AU2003227872A1 (enExample)
CA (1) CA2484397C (enExample)
DE (1) DE60314395T2 (enExample)
GB (2) GB0208987D0 (enExample)
IL (1) IL164543A0 (enExample)
WO (1) WO2003089665A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090118139A1 (en) * 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
EP1467211B1 (de) * 2003-04-10 2008-04-30 Tecan Trading AG Verfahren zum Messen von an biologische Moleküle gebundenen, chemischen Gruppen
WO2005043137A1 (de) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer
WO2006132657A2 (en) * 2004-09-22 2006-12-14 Applera Corporation Enzyme assay with nanowire sensor
WO2007049057A2 (en) * 2005-10-28 2007-05-03 Iti Scotland Limited Novel fluorescent dyes and uses thereof
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
FI20070508A0 (fi) * 2007-06-27 2007-06-27 Turun Yliopisto Peptidisubstraatti ja sen käyttö
DE102008011578A1 (de) * 2008-02-28 2009-09-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Photolumineszenz-Sensor
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032381A (en) * 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
DE69739898D1 (de) * 1996-07-16 2010-07-15 Univ California Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten
JP3815051B2 (ja) 1997-04-30 2006-08-30 和光純薬工業株式会社 新規なペプチド誘導体
ATE233325T1 (de) * 1997-12-05 2003-03-15 Upjohn Co Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
JP2001019700A (ja) 1999-07-07 2001-01-23 Mitsubishi Chemicals Corp 蛍光標識ペプチド
ATE291096T1 (de) * 1999-07-30 2005-04-15 Bayer Ag Chemilumineszenz-substrate von hydrolytischen enzymen wie phosphatasen
EP1264897A3 (en) 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetic sensor peptide for kinase or phosphatase assays

Also Published As

Publication number Publication date
GB2387905A (en) 2003-10-29
GB0208987D0 (en) 2002-05-29
GB2387905B (en) 2005-01-12
JP4615867B2 (ja) 2011-01-19
GB0308903D0 (en) 2003-05-21
JP2005523024A (ja) 2005-08-04
CA2484397A1 (en) 2003-10-30
ATE364715T1 (de) 2007-07-15
US20030228646A1 (en) 2003-12-11
CA2484397C (en) 2014-06-17
EP1497453A1 (en) 2005-01-19
US7125682B2 (en) 2006-10-24
WO2003089665A1 (en) 2003-10-30
DE60314395D1 (de) 2007-07-26
IL164543A0 (en) 2005-12-18
EP1497453B1 (en) 2007-06-13
DE60314395T2 (de) 2008-02-21
WO2003089665A8 (en) 2004-11-18

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Legal Events

Date Code Title Description
TC Change of applicant's name (sec. 104)

Owner name: GE HEALTHCARE UK LIMITED

Free format text: FORMER NAME: AMERSHAM BIO-SCIENCES UK LIMITED

MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted