JP4615867B2 - プロテインキナーゼ及びホスファターゼ活性の測定法 - Google Patents

プロテインキナーゼ及びホスファターゼ活性の測定法 Download PDF

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Publication number
JP4615867B2
JP4615867B2 JP2003586374A JP2003586374A JP4615867B2 JP 4615867 B2 JP4615867 B2 JP 4615867B2 JP 2003586374 A JP2003586374 A JP 2003586374A JP 2003586374 A JP2003586374 A JP 2003586374A JP 4615867 B2 JP4615867 B2 JP 4615867B2
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substrate
enzyme
group
atoms
phosphorylated
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Japanese (ja)
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JP2005523024A (ja
JP2005523024A5 (enExample
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ホエートレイ，ジョン・ジェラルド
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ジーイー・ヘルスケア・ユーケイ・リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2003586374A 2002-04-19 2003-04-17 プロテインキナーゼ及びホスファターゼ活性の測定法 Expired - Fee Related JP4615867B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0208987.8A GB0208987D0 (en) 2002-04-19 2002-04-19 Methods for measuring protein kinase and phosphatase activity
PCT/GB2003/001683 WO2003089665A1 (en) 2002-04-19 2003-04-17 Methods for measuring protein kinase and phosphatase activity

Publications (3)

Publication Number Publication Date
JP2005523024A JP2005523024A (ja) 2005-08-04
JP2005523024A5 JP2005523024A5 (enExample) 2009-12-17
JP4615867B2 true JP4615867B2 (ja) 2011-01-19

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ID=9935150

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JP2003586374A Expired - Fee Related JP4615867B2 (ja) 2002-04-19 2003-04-17 プロテインキナーゼ及びホスファターゼ活性の測定法

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Country Link
US (1) US7125682B2 (enExample)
EP (1) EP1497453B1 (enExample)
JP (1) JP4615867B2 (enExample)
AT (1) ATE364715T1 (enExample)
AU (1) AU2003227872A1 (enExample)
CA (1) CA2484397C (enExample)
DE (1) DE60314395T2 (enExample)
GB (2) GB0208987D0 (enExample)
IL (1) IL164543A0 (enExample)
WO (1) WO2003089665A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090118139A1 (en) * 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
EP1467211B1 (de) * 2003-04-10 2008-04-30 Tecan Trading AG Verfahren zum Messen von an biologische Moleküle gebundenen, chemischen Gruppen
WO2005043137A1 (de) * 2003-10-18 2005-05-12 Bayer Healthcare Ag Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer
WO2006132657A2 (en) * 2004-09-22 2006-12-14 Applera Corporation Enzyme assay with nanowire sensor
WO2007049057A2 (en) * 2005-10-28 2007-05-03 Iti Scotland Limited Novel fluorescent dyes and uses thereof
GB0712109D0 (en) * 2007-06-22 2007-08-01 Edinburgh Instr Fluorescence lifetime and fluorescence assays
FI20070508A0 (fi) * 2007-06-27 2007-06-27 Turun Yliopisto Peptidisubstraatti ja sen käyttö
DE102008011578A1 (de) * 2008-02-28 2009-09-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Photolumineszenz-Sensor
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5032381A (en) * 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
DE69739898D1 (de) * 1996-07-16 2010-07-15 Univ California Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten
JP3815051B2 (ja) 1997-04-30 2006-08-30 和光純薬工業株式会社 新規なペプチド誘導体
ATE233325T1 (de) * 1997-12-05 2003-03-15 Upjohn Co Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
JP2001019700A (ja) 1999-07-07 2001-01-23 Mitsubishi Chemicals Corp 蛍光標識ペプチド
ATE291096T1 (de) * 1999-07-30 2005-04-15 Bayer Ag Chemilumineszenz-substrate von hydrolytischen enzymen wie phosphatasen
EP1264897A3 (en) 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetic sensor peptide for kinase or phosphatase assays

Also Published As

Publication number Publication date
GB2387905A (en) 2003-10-29
GB0208987D0 (en) 2002-05-29
GB2387905B (en) 2005-01-12
GB0308903D0 (en) 2003-05-21
JP2005523024A (ja) 2005-08-04
CA2484397A1 (en) 2003-10-30
ATE364715T1 (de) 2007-07-15
AU2003227872A1 (en) 2003-11-03
US20030228646A1 (en) 2003-12-11
CA2484397C (en) 2014-06-17
EP1497453A1 (en) 2005-01-19
US7125682B2 (en) 2006-10-24
WO2003089665A1 (en) 2003-10-30
DE60314395D1 (de) 2007-07-26
IL164543A0 (en) 2005-12-18
EP1497453B1 (en) 2007-06-13
DE60314395T2 (de) 2008-02-21
WO2003089665A8 (en) 2004-11-18

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