IL164543A0 - Methods for measuring protein kinase and phosphatase activity - Google Patents
Methods for measuring protein kinase and phosphatase activityInfo
- Publication number
- IL164543A0 IL164543A0 IL16454304A IL16454304A IL164543A0 IL 164543 A0 IL164543 A0 IL 164543A0 IL 16454304 A IL16454304 A IL 16454304A IL 16454304 A IL16454304 A IL 16454304A IL 164543 A0 IL164543 A0 IL 164543A0
- Authority
- IL
- Israel
- Prior art keywords
- substrate
- labelled
- enzyme
- methods
- dye
- Prior art date
Links
- 230000000694 effects Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 title 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 title 1
- 102000001253 Protein Kinase Human genes 0.000 title 1
- 108060006633 protein kinase Proteins 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 6
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 239000007850 fluorescent dye Substances 0.000 abstract 2
- 230000000865 phosphorylative effect Effects 0.000 abstract 2
- GDALETGZDYOOGB-UHFFFAOYSA-N Acridone Natural products C1=C(O)C=C2N(C)C3=CC=CC=C3C(=O)C2=C1O GDALETGZDYOOGB-UHFFFAOYSA-N 0.000 abstract 1
- NRCMAYZCPIVABH-UHFFFAOYSA-N Quinacridone Chemical compound N1C2=CC=CC=C2C(=O)C2=C1C=C1C(=O)C3=CC=CC=C3NC1=C2 NRCMAYZCPIVABH-UHFFFAOYSA-N 0.000 abstract 1
- FZEYVTFCMJSGMP-UHFFFAOYSA-N acridone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3NC2=C1 FZEYVTFCMJSGMP-UHFFFAOYSA-N 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000030609 dephosphorylation Effects 0.000 abstract 1
- 238000006209 dephosphorylation reaction Methods 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 230000026731 phosphorylation Effects 0.000 abstract 1
- 238000006366 phosphorylation reaction Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0208987.8A GB0208987D0 (en) | 2002-04-19 | 2002-04-19 | Methods for measuring protein kinase and phosphatase activity |
| PCT/GB2003/001683 WO2003089665A1 (en) | 2002-04-19 | 2003-04-17 | Methods for measuring protein kinase and phosphatase activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL164543A0 true IL164543A0 (en) | 2005-12-18 |
Family
ID=9935150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL16454304A IL164543A0 (en) | 2002-04-19 | 2004-10-13 | Methods for measuring protein kinase and phosphatase activity |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7125682B2 (xx) |
| EP (1) | EP1497453B1 (xx) |
| JP (1) | JP4615867B2 (xx) |
| AT (1) | ATE364715T1 (xx) |
| AU (1) | AU2003227872A1 (xx) |
| CA (1) | CA2484397C (xx) |
| DE (1) | DE60314395T2 (xx) |
| GB (2) | GB0208987D0 (xx) |
| IL (1) | IL164543A0 (xx) |
| WO (1) | WO2003089665A1 (xx) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090118139A1 (en) * | 2000-11-07 | 2009-05-07 | Caliper Life Sciences, Inc. | Microfluidic method and system for enzyme inhibition activity screening |
| DE502004006979D1 (de) * | 2003-04-10 | 2008-06-12 | Tecan Trading Ag | Verfahren zum Messen von an biologische Moleküle gebundenen, chemischen Gruppen |
| SG147471A1 (en) * | 2003-10-18 | 2008-11-28 | Bayer Healthcare Ag | Direct observation of molecular modifications in biological test systems by measuring fluorescence lifetime |
| US20060084128A1 (en) * | 2004-09-22 | 2006-04-20 | Hongye Sun | Enzyme assay with nanowire sensor |
| EP1940965B1 (en) * | 2005-10-28 | 2014-03-26 | Almac Sciences (Scotland) Limited | Novel fluorescent dyes and uses thereof |
| GB0712109D0 (en) * | 2007-06-22 | 2007-08-01 | Edinburgh Instr | Fluorescence lifetime and fluorescence assays |
| FI20070508A0 (fi) * | 2007-06-27 | 2007-06-27 | Turun Yliopisto | Peptidisubstraatti ja sen käyttö |
| DE102008011578A1 (de) * | 2008-02-28 | 2009-09-03 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG | Photolumineszenz-Sensor |
| GB201122099D0 (en) | 2011-12-22 | 2012-02-01 | Almac Sciences Scotland Ltd | Dyes |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5032381A (en) * | 1988-12-20 | 1991-07-16 | Tropix, Inc. | Chemiluminescence-based static and flow cytometry |
| US6803188B1 (en) * | 1996-01-31 | 2004-10-12 | The Regents Of The University Of California | Tandem fluorescent protein constructs |
| AU3801997A (en) * | 1996-07-16 | 1998-02-09 | Regents Of The University Of California, The | Assays for protein kinases using fluorescent protein substrates |
| JP3815051B2 (ja) | 1997-04-30 | 2006-08-30 | 和光純薬工業株式会社 | 新規なペプチド誘導体 |
| ES2192801T3 (es) * | 1997-12-05 | 2003-10-16 | Upjohn Co | Ensayos de criba de alta productividad de proteina quinasas y fosfatasas, basados en fluorescencia. |
| JP2001019700A (ja) | 1999-07-07 | 2001-01-23 | Mitsubishi Chemicals Corp | 蛍光標識ペプチド |
| AU6381900A (en) * | 1999-07-30 | 2001-02-19 | Bayer Corporation | Chemiluminescent substrates of hydrolytic enzymes such as phosphatases |
| EP1264897A3 (en) | 2001-06-06 | 2003-11-12 | Europäisches Laboratorium Für Molekularbiologie (Embl) | Synthetic sensor peptide for kinase or phosphatase assays |
-
2002
- 2002-04-19 GB GBGB0208987.8A patent/GB0208987D0/en not_active Ceased
-
2003
- 2003-04-17 WO PCT/GB2003/001683 patent/WO2003089665A1/en active IP Right Grant
- 2003-04-17 AT AT03725332T patent/ATE364715T1/de not_active IP Right Cessation
- 2003-04-17 GB GB0308903A patent/GB2387905B/en not_active Expired - Lifetime
- 2003-04-17 AU AU2003227872A patent/AU2003227872A1/en not_active Abandoned
- 2003-04-17 JP JP2003586374A patent/JP4615867B2/ja not_active Expired - Fee Related
- 2003-04-17 DE DE60314395T patent/DE60314395T2/de not_active Expired - Lifetime
- 2003-04-17 EP EP03725332A patent/EP1497453B1/en not_active Expired - Lifetime
- 2003-04-17 CA CA2484397A patent/CA2484397C/en not_active Expired - Fee Related
- 2003-04-17 US US10/417,545 patent/US7125682B2/en active Active
-
2004
- 2004-10-13 IL IL16454304A patent/IL164543A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003227872A1 (en) | 2003-11-03 |
| CA2484397C (en) | 2014-06-17 |
| GB2387905A (en) | 2003-10-29 |
| GB2387905B (en) | 2005-01-12 |
| EP1497453B1 (en) | 2007-06-13 |
| ATE364715T1 (de) | 2007-07-15 |
| JP4615867B2 (ja) | 2011-01-19 |
| EP1497453A1 (en) | 2005-01-19 |
| US20030228646A1 (en) | 2003-12-11 |
| DE60314395D1 (de) | 2007-07-26 |
| GB0208987D0 (en) | 2002-05-29 |
| GB0308903D0 (en) | 2003-05-21 |
| CA2484397A1 (en) | 2003-10-30 |
| US7125682B2 (en) | 2006-10-24 |
| WO2003089665A1 (en) | 2003-10-30 |
| DE60314395T2 (de) | 2008-02-21 |
| JP2005523024A (ja) | 2005-08-04 |
| WO2003089665A8 (en) | 2004-11-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yasgar et al. | AlphaScreen-based assays: ultra-high-throughput screening for small-molecule inhibitors of challenging enzymes and protein-protein interactions | |
| Baki et al. | A high throughput luminescent assay for glycogen synthase kinase-3 β inhibitors | |
| Wang et al. | Protein kinase profiling assays: a technology review | |
| AU2001279222B2 (en) | Multiplexed enzymatic assays | |
| Li et al. | Fluorescence detection techniques for protein kinase assay | |
| GB2387905B (en) | Methods for measuring protein kinase and phosphatase activity | |
| EP2812698B1 (en) | Dual-acceptor time-resolved-fret | |
| AU2001279222A1 (en) | Multiplexed enzymatic assays | |
| US20070009978A1 (en) | Three part assay for kinase or phosphatase activity | |
| Wang et al. | Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis | |
| Zhang et al. | Highly selective in vivo imaging of endogenous/exogenous phosphate ion over ATP and PPi | |
| Klumpp et al. | Readout technologies for highly miniaturized kinase assays applicable to high-throughput screening in a 1536-well format | |
| Pommereau et al. | Two simple and generic antibody-independent kinase assays: comparison of a bioluminescent and a microfluidic assay format | |
| US6265179B1 (en) | Detection of phosphate using coupled enzymatic reactions | |
| Niles et al. | Multiplexed viability, cytotoxicity, and caspase activity assays | |
| US20140273036A1 (en) | Luminescent detection of inorganic phosphate and coupled reactions | |
| KR20010033496A (ko) | 형광분석계용 조성물 | |
| WO2009001051A3 (en) | Fluorescence lifetime and fluorescence assays | |
| Jena et al. | Fluorescence lifetime imaging probes for cell-based measurements of enzyme activity | |
| EP1678483A1 (de) | Direkte beobachtung molekularer veränderungen in biologischen testsystemen mittels messungen der fluoreszenz-lebensdauer | |
| DE10348949A1 (de) | Direkte Beobachtung molekularer Veränderungen ind biologischen Testsystemen mittels Messungen der Fluoreszenz-Lebensdauer | |
| US7901901B2 (en) | Assays for measuring phosphate modification enzyme activity | |
| Miller | Advances in fluorescence enzyme detection methods | |
| US7223610B2 (en) | Direct phosphorylation state monitoring on biomolecules | |
| O'Shea et al. | Homogeneous time-resolved fluorescence assays for the detection of activity and inhibition of phosphatase enzymes employing phosphorescently labeled peptide substrates |