DE60231214D1 - Effizientes nachweisverfahren für doppelsträngige nukleinsäure - Google Patents
Effizientes nachweisverfahren für doppelsträngige nukleinsäureInfo
- Publication number
- DE60231214D1 DE60231214D1 DE60231214T DE60231214T DE60231214D1 DE 60231214 D1 DE60231214 D1 DE 60231214D1 DE 60231214 T DE60231214 T DE 60231214T DE 60231214 T DE60231214 T DE 60231214T DE 60231214 D1 DE60231214 D1 DE 60231214D1
- Authority
- DE
- Germany
- Prior art keywords
- intercalater
- stranded
- bonded
- double
- reducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 21
- 102000039446 nucleic acids Human genes 0.000 title abstract 21
- 108020004707 nucleic acids Proteins 0.000 title abstract 21
- 238000000034 method Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 230000027455 binding Effects 0.000 abstract 2
- 230000009870 specific binding Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001183716 | 2001-06-18 | ||
| PCT/JP2002/005739 WO2002103053A1 (en) | 2001-06-18 | 2002-06-10 | Method of efficiently detecting double-stranded nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60231214D1 true DE60231214D1 (de) | 2009-04-02 |
Family
ID=19023614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60231214T Expired - Lifetime DE60231214D1 (de) | 2001-06-18 | 2002-06-10 | Effizientes nachweisverfahren für doppelsträngige nukleinsäure |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US7316901B2 (de) |
| EP (2) | EP1416055B1 (de) |
| JP (1) | JP4081435B2 (de) |
| CN (2) | CN1810992B (de) |
| AT (2) | ATE524560T1 (de) |
| DE (1) | DE60231214D1 (de) |
| ES (2) | ES2373234T3 (de) |
| HK (2) | HK1092182A1 (de) |
| WO (1) | WO2002103053A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1151142A2 (de) * | 1999-01-28 | 2001-11-07 | Gen-Probe Incorporated | Nukleinsäure sequenzen für den nachweis von genetischen markern für krebs in einer biologischen probe |
| US7223604B1 (en) * | 2002-09-27 | 2007-05-29 | Science & Technology Corporation @ Unm | Methods and kits for the detection of erythrocytes |
| JP2006296279A (ja) * | 2005-04-20 | 2006-11-02 | Sony Corp | インターカレーターを用いるハイブリダイゼーション検出方法 |
| TWI402348B (zh) | 2007-05-28 | 2013-07-21 | Univ Ehime | 用於偵測瘧原蟲之引子 |
| SG175699A1 (en) * | 2009-03-19 | 2011-12-29 | Kaneka Corp | Method, kit and device for detecting nucleic acid |
| US20130029340A1 (en) * | 2010-01-15 | 2013-01-31 | Steffen Mergemeier | Method for detecting more than one target in a pcr-based approach applying an un-specific dye which is not interfering with the emission of fluorophore-labeled probes |
| SG188978A1 (en) | 2010-09-22 | 2013-05-31 | Kaneka Corp | Method and device for detecting nucleic acid, and kit |
| KR101904787B1 (ko) | 2011-03-04 | 2018-10-08 | 가부시키가이샤 가네카 | 핵산의 검출 방법 및 그 방법에 사용하는 디바이스, 키트 |
| CN104089934B (zh) * | 2014-07-11 | 2016-06-15 | 北京科技大学 | 一种用于dna荧光检测的复合纳米银薄膜的制备方法 |
| JPWO2021117667A1 (de) * | 2019-12-09 | 2021-06-17 | ||
| US11788129B2 (en) | 2021-02-23 | 2023-10-17 | Industrial Technology Research Institute | Nucleic acid detection kit and nucleic acid detection method |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4257774A (en) * | 1979-07-16 | 1981-03-24 | Meloy Laboratories, Inc. | Intercalation inhibition assay for compounds that interact with DNA or RNA |
| US5998135A (en) * | 1989-02-24 | 1999-12-07 | Enzo Diagnostics, Inc. | Energy transfer hybridization assay using intercalators and lanthanide metals |
| US5340716A (en) * | 1991-06-20 | 1994-08-23 | Snytex (U.S.A.) Inc. | Assay method utilizing photoactivated chemiluminescent label |
| DE69718268D1 (de) * | 1996-10-03 | 2003-02-13 | Canon Kk | Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse |
| WO1998036096A1 (en) | 1997-02-14 | 1998-08-20 | E.I. Du Pont De Nemours And Company | Detection of double-stranded dna in a homogeneous solution |
| US6063573A (en) * | 1998-01-27 | 2000-05-16 | Clinical Micro Sensors, Inc. | Cycling probe technology using electron transfer detection |
| JP4306881B2 (ja) * | 1999-07-26 | 2009-08-05 | キヤノン株式会社 | 乾式蛍光測定による標的核酸の検出／定量方法 |
| US6960432B2 (en) * | 1999-07-26 | 2005-11-01 | Canon Kabushiki Kaisha | Detection/quantification of targeted nucleotide chains, and detection/quantification of multi-stranded nucleotide chains by fluorescence |
| US6506567B2 (en) * | 2000-01-31 | 2003-01-14 | Fuji Photo Film Co., Ltd. | Water-soluble flourescent intercalator compound |
| JP4267208B2 (ja) * | 2000-01-31 | 2009-05-27 | 富士フイルム株式会社 | 蛍光インターカレータおよび相補性核酸断片の検出方法 |
| JP2001298655A (ja) * | 2000-04-13 | 2001-10-26 | Sony Corp | 撮像装置および方法、並びに記録媒体 |
-
2002
- 2002-06-10 US US10/481,369 patent/US7316901B2/en not_active Expired - Lifetime
- 2002-06-10 JP JP2003505374A patent/JP4081435B2/ja not_active Expired - Lifetime
- 2002-06-10 WO PCT/JP2002/005739 patent/WO2002103053A1/ja active Application Filing
- 2002-06-10 EP EP02733442A patent/EP1416055B1/de not_active Expired - Lifetime
- 2002-06-10 DE DE60231214T patent/DE60231214D1/de not_active Expired - Lifetime
- 2002-06-10 ES ES06016881T patent/ES2373234T3/es not_active Expired - Lifetime
- 2002-06-10 CN CN200510116484.7A patent/CN1810992B/zh not_active Expired - Lifetime
- 2002-06-10 CN CN02815992.6A patent/CN1283807C/zh not_active Expired - Lifetime
- 2002-06-10 ES ES02733442T patent/ES2322040T3/es not_active Expired - Lifetime
- 2002-06-10 AT AT06016881T patent/ATE524560T1/de not_active IP Right Cessation
- 2002-06-10 EP EP06016881A patent/EP1724361B1/de not_active Expired - Lifetime
- 2002-06-10 AT AT02733442T patent/ATE423222T1/de not_active IP Right Cessation
-
2005
- 2005-03-17 HK HK06112562.7A patent/HK1092182A1/xx not_active IP Right Cessation
- 2005-03-17 HK HK05102344A patent/HK1070104A1/xx not_active IP Right Cessation
-
2007
- 2007-11-07 US US11/936,588 patent/US20080145854A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP4081435B2 (ja) | 2008-04-23 |
| CN1283807C (zh) | 2006-11-08 |
| CN1810992B (zh) | 2010-09-08 |
| CN1810992A (zh) | 2006-08-02 |
| HK1070104A1 (en) | 2005-06-10 |
| EP1724361B1 (de) | 2011-09-14 |
| EP1416055A4 (de) | 2004-10-13 |
| ES2322040T3 (es) | 2009-06-16 |
| EP1416055B1 (de) | 2009-02-18 |
| EP1724361A1 (de) | 2006-11-22 |
| ATE524560T1 (de) | 2011-09-15 |
| ATE423222T1 (de) | 2009-03-15 |
| US20080145854A1 (en) | 2008-06-19 |
| HK1092182A1 (en) | 2007-02-02 |
| US20040171016A1 (en) | 2004-09-02 |
| US7316901B2 (en) | 2008-01-08 |
| ES2373234T3 (es) | 2012-02-01 |
| EP1416055A1 (de) | 2004-05-06 |
| JPWO2002103053A1 (ja) | 2004-09-30 |
| CN1543509A (zh) | 2004-11-03 |
| WO2002103053A1 (en) | 2002-12-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |