ATE290607T1 - Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren - Google Patents

Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren

Info

Publication number
ATE290607T1
ATE290607T1 AT99961232T AT99961232T ATE290607T1 AT E290607 T1 ATE290607 T1 AT E290607T1 AT 99961232 T AT99961232 T AT 99961232T AT 99961232 T AT99961232 T AT 99961232T AT E290607 T1 ATE290607 T1 AT E290607T1
Authority
AT
Austria
Prior art keywords
fluorophore
binding
compound
labeled compound
protein
Prior art date
Application number
AT99961232T
Other languages
English (en)
Inventor
Glen H Erikson
Jasmine I Daksis
Original Assignee
Ingeneus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp filed Critical Ingeneus Corp
Application granted granted Critical
Publication of ATE290607T1 publication Critical patent/ATE290607T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT99961232T 1998-12-31 1999-12-29 Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren ATE290607T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/224,505 US6294333B1 (en) 1998-12-31 1998-12-31 Fluorescent intensity assay for protein or peptide binding to nucleic acids
PCT/IB1999/002066 WO2000040753A1 (en) 1998-12-31 1999-12-29 Fluorescent intensity assay for protein or peptide binding to nucleic acids

Publications (1)

Publication Number Publication Date
ATE290607T1 true ATE290607T1 (de) 2005-03-15

Family

ID=22840991

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99961232T ATE290607T1 (de) 1998-12-31 1999-12-29 Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren

Country Status (9)

Country Link
US (1) US6294333B1 (de)
EP (1) EP1141392B1 (de)
JP (1) JP3959438B2 (de)
AT (1) ATE290607T1 (de)
AU (1) AU771451B2 (de)
CA (1) CA2358065C (de)
DE (1) DE69924147T2 (de)
TW (1) TWI235766B (de)
WO (1) WO2000040753A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US7172865B2 (en) 2001-08-13 2007-02-06 Saint Louis University Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
US6544746B2 (en) 2001-08-13 2003-04-08 St. Louis University Rapid and sensitive proximity-based assay for the detection and quantification of DNA binding proteins
JP2003284552A (ja) * 2002-03-28 2003-10-07 Hitachi Software Eng Co Ltd イオン性高分子同定用高分子チップ及びイオン性高分子の同定方法
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
JP3916086B2 (ja) * 2004-06-29 2007-05-16 ソニー株式会社 ハイブリダイゼーションなどの相互作用を検出する方法及び検出部、該検出部を備えるバイオアッセイ用基板、ハイブリダイゼーションなどの相互作用を検出する装置、並びに試薬キット
KR20070062575A (ko) * 2004-09-24 2007-06-15 인제네우스 인크. 게놈 분석
WO2010088282A1 (en) * 2009-01-30 2010-08-05 Rgo Biosciences Llc Nucleic acid binding assays
KR101341640B1 (ko) * 2010-02-05 2013-12-16 한국전자통신연구원 단백질 미세 구조 분석 방법 및 단백질 미세 구조 분석용 어레이

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0103469B1 (de) 1982-09-10 1989-02-08 The Welsh National School of Medicine Immunologisches Verfahren zur Bestimmung von Stoffen
US4743535A (en) * 1984-11-07 1988-05-10 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid
US5187106A (en) 1984-08-30 1993-02-16 Orbit Medical Systems, Inc. Method and apparatus for homogeneous fluorescence measurements
US5726014A (en) 1991-06-27 1998-03-10 Genelabs Technologies, Inc. Screening assay for the detection of DNA-binding molecules
US5783384A (en) 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
US5747247A (en) * 1994-07-25 1998-05-05 The Regents Of The University Of California Spectroscopic helicase assay
US6107029A (en) 1996-07-31 2000-08-22 Message Pharmaceticals, Inc. Universal method for detecting interactions between RNA molecules and RNA binding proteins
AU7847798A (en) 1996-12-13 1998-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization
US5846729A (en) 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect

Also Published As

Publication number Publication date
DE69924147T2 (de) 2006-01-05
DE69924147D1 (de) 2005-04-14
JP2002534664A (ja) 2002-10-15
CA2358065A1 (en) 2000-07-13
US6294333B1 (en) 2001-09-25
JP3959438B2 (ja) 2007-08-15
WO2000040753A1 (en) 2000-07-13
AU1791100A (en) 2000-07-24
AU771451B2 (en) 2004-03-25
CA2358065C (en) 2007-05-01
TWI235766B (en) 2005-07-11
EP1141392B1 (de) 2005-03-09
EP1141392A1 (de) 2001-10-10

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