DE60128720T2 - Mikroarray-verfahren zur genotypisierung mehrerer proben für mehrere loci - Google Patents
Mikroarray-verfahren zur genotypisierung mehrerer proben für mehrere loci Download PDFInfo
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- DE60128720T2 DE60128720T2 DE60128720T DE60128720T DE60128720T2 DE 60128720 T2 DE60128720 T2 DE 60128720T2 DE 60128720 T DE60128720 T DE 60128720T DE 60128720 T DE60128720 T DE 60128720T DE 60128720 T2 DE60128720 T2 DE 60128720T2
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- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/613,006 US6913879B1 (en) | 2000-07-10 | 2000-07-10 | Microarray method of genotyping multiple samples at multiple LOCI |
| US613006 | 2000-07-10 | ||
| PCT/US2001/021163 WO2002003849A2 (en) | 2000-07-10 | 2001-07-03 | Microarray method of genotyping multiple samples at multiple loci |
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| Publication Number | Publication Date |
|---|---|
| DE60128720D1 DE60128720D1 (de) | 2007-07-12 |
| DE60128720T2 true DE60128720T2 (de) | 2008-01-24 |
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|---|---|
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| EP (1) | EP1343911B1 (enExample) |
| JP (1) | JP4175884B2 (enExample) |
| KR (1) | KR100756015B1 (enExample) |
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| AT (1) | ATE363543T1 (enExample) |
| AU (2) | AU2002218740B2 (enExample) |
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| TW (1) | TWI280282B (enExample) |
| WO (1) | WO2002003849A2 (enExample) |
Families Citing this family (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10000001A1 (de) * | 2000-01-01 | 2001-07-19 | Agrobiogen Gmbh | DNA-Matrizes und deren Verwendung zur Untersuchung von Individuen einer Population |
| US6913879B1 (en) * | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
| GB0208364D0 (en) * | 2002-04-11 | 2002-05-22 | Sec Dep For The Home Departmen | Improvements in and relating to methods and apparatus for genotyping |
| WO2004044242A1 (en) * | 2002-11-14 | 2004-05-27 | John Nicholas Housby | Polymorphism assay |
| US7097070B2 (en) | 2003-08-15 | 2006-08-29 | Protedyne Corporation | Method and apparatus for handling small volume fluid samples |
| JP4458327B2 (ja) * | 2003-08-28 | 2010-04-28 | キヤノン株式会社 | 標的物質の定量方法および該方法に用いるプローブ担体 |
| US7108979B2 (en) * | 2003-09-03 | 2006-09-19 | Agilent Technologies, Inc. | Methods to detect cross-contamination between samples contacted with a multi-array substrate |
| CN101061223A (zh) * | 2004-11-19 | 2007-10-24 | 株式会社岛津制作所 | 基因多态性检测方法、诊断方法以及用于其的装置及检验试剂盒 |
| RU2348695C2 (ru) | 2006-05-23 | 2009-03-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа |
| GB0618514D0 (en) * | 2006-09-20 | 2006-11-01 | Univ Nottingham Trent | Method of detecting interactions on a microarray using nuclear magnetic resonance |
| EP2099935B1 (en) * | 2006-11-30 | 2014-02-26 | The Regents Of The University Of California | Array for detecting microbes |
| BRPI0811271A2 (pt) * | 2007-05-31 | 2015-01-20 | Monsanto Technology Llc | Polimorfismos da soja e processos de genotipagem |
| WO2009029771A2 (en) | 2007-08-29 | 2009-03-05 | Monsanto Technology Llc | Methods and compositions for breeding for preferred traits |
| ES2936256T3 (es) | 2008-02-01 | 2023-03-15 | Massachusetts Gen Hospital | Uso de microvesículas en el diagnóstico, y pronóstico de enfermedades y afecciones médicas |
| CN104388568B (zh) | 2008-04-24 | 2017-04-12 | 孟山都技术有限公司 | 鉴定大豆中亚洲大豆锈病抗性数量性状基因座的方法和其组合物 |
| US9393566B2 (en) * | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
| EP2425240A4 (en) | 2009-04-30 | 2012-12-12 | Good Start Genetics Inc | METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS |
| US12129514B2 (en) | 2009-04-30 | 2024-10-29 | Molecular Loop Biosolutions, Llc | Methods and compositions for evaluating genetic markers |
| US20100299773A1 (en) * | 2009-05-20 | 2010-11-25 | Monsanto Technology Llc | Methods and compositions for selecting an improved plant |
| IN2012DN00866A (enExample) | 2009-07-16 | 2015-07-10 | Gen Hospital Corp | |
| WO2011031877A1 (en) | 2009-09-09 | 2011-03-17 | The General Hospital Corporation | Use of microvesicles in analyzing nucleic acid profiles |
| WO2011031892A1 (en) | 2009-09-09 | 2011-03-17 | The General Hospital Corporation | Use of microvesicles in analyzing kras mutations |
| WO2012031008A2 (en) | 2010-08-31 | 2012-03-08 | The General Hospital Corporation | Cancer-related biological materials in microvesicles |
| EP2638057B1 (en) | 2010-11-10 | 2019-03-06 | Exosome Diagnostics, Inc. | Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| WO2012149193A2 (en) | 2011-04-29 | 2012-11-01 | Monsanto Technology Llc | Diagnostic molecular markers for seed lot purity traits in soybeans |
| ES2744589T3 (es) | 2011-05-11 | 2020-02-25 | Exosome Diagnostics Inc | Extracción de ácido nucleico de materiales biológicos heterogéneos |
| WO2013028788A1 (en) | 2011-08-22 | 2013-02-28 | Exosome Diagnostics, Inc. | Urine biomarkers |
| HUE059863T2 (hu) | 2011-08-31 | 2023-01-28 | Seminis Vegetable Seeds Inc | Eljárás és készítmények görögdinnye keménységhez |
| EP2768983A4 (en) | 2011-10-17 | 2015-06-03 | Good Start Genetics Inc | METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES |
| US10174361B2 (en) | 2011-11-10 | 2019-01-08 | Exosome Diagnostics, Inc. | Cerebrospinal fluid assay |
| CN108611398A (zh) | 2012-01-13 | 2018-10-02 | Data生物有限公司 | 通过新一代测序进行基因分型 |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| EP2890783A4 (en) | 2012-08-30 | 2016-03-02 | Exosome Diagnostics Inc | WITNESSES FOR NUCLEIC ACID ASSAYS |
| CN105025878A (zh) | 2012-10-03 | 2015-11-04 | 外来体诊断公司 | 微泡在医学疾病和病况的诊断、预后和治疗中的用途 |
| US20140127688A1 (en) | 2012-11-07 | 2014-05-08 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| GB201220924D0 (en) | 2012-11-21 | 2013-01-02 | Cancer Res Inst Royal | Materials and methods for determining susceptibility or predisposition to cancer |
| HUE039597T2 (hu) * | 2012-12-03 | 2019-01-28 | Statens Seruminstitut | Eljárás galaktozémia kimutatására újszülöttek szûrésekor |
| US10314253B2 (en) | 2012-12-04 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and compositions for watermelon sex expression |
| US8778609B1 (en) | 2013-03-14 | 2014-07-15 | Good Start Genetics, Inc. | Methods for analyzing nucleic acids |
| US10294489B2 (en) | 2013-03-15 | 2019-05-21 | Board Of Trustees Of Southern Illinois University | Soybean resistant to cyst nematodes |
| EP3005200A2 (en) | 2013-06-03 | 2016-04-13 | Good Start Genetics, Inc. | Methods and systems for storing sequence read data |
| US10059999B2 (en) | 2013-06-10 | 2018-08-28 | Monsanto Technology Llc | Molecular markers associated with soybean tolerance to low iron growth conditions |
| US10301681B2 (en) | 2013-08-06 | 2019-05-28 | Exosome Diagnostics, Inc. | Methods of treating a subject with a high gleason score prostate cancer |
| NZ630628A (en) | 2013-10-08 | 2015-04-24 | Seminis Vegetable Seeds Inc | Methods and compositions for peronospora resistance in spinach |
| US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| WO2015057565A1 (en) | 2013-10-18 | 2015-04-23 | Good Start Genetics, Inc. | Methods for assessing a genomic region of a subject |
| NZ767892A (en) | 2013-11-27 | 2022-07-01 | Seminis Vegetable Seeds Inc | Disease resistance loci in onion |
| PT3632202T (pt) | 2014-02-21 | 2022-10-03 | Syngenta Participations Ag | Locais genéticos associados a fertilidade acrescida em milho |
| NZ630710A (en) | 2014-02-27 | 2016-03-31 | Seminis Vegetable Seeds Inc | Compositions and methods for peronospora resistance in spinach |
| WO2015175530A1 (en) | 2014-05-12 | 2015-11-19 | Gore Athurva | Methods for detecting aneuploidy |
| US10316369B2 (en) | 2014-06-27 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and assays for male sterile watermelon |
| WO2016025818A1 (en) | 2014-08-15 | 2016-02-18 | Good Start Genetics, Inc. | Systems and methods for genetic analysis |
| US11408024B2 (en) | 2014-09-10 | 2022-08-09 | Molecular Loop Biosciences, Inc. | Methods for selectively suppressing non-target sequences |
| JP2017536087A (ja) | 2014-09-24 | 2017-12-07 | グッド スタート ジェネティクス， インコーポレイテッド | 遺伝子アッセイのロバストネスを増大させるためのプロセス制御 |
| EP3005862A1 (en) | 2014-10-10 | 2016-04-13 | Seminis Vegetable Seeds, Inc. | Melon plants with improved disease tolerance |
| US10066259B2 (en) | 2015-01-06 | 2018-09-04 | Good Start Genetics, Inc. | Screening for structural variants |
| CA3285074A1 (en) | 2015-04-28 | 2026-03-02 | Monsanto Technology Llc | Methods and compositions for producing brachytic corn plants |
| UA126326C2 (uk) | 2015-04-30 | 2022-09-21 | Монсанто Текнолоджі Елелсі | Спосіб відбору рослини каноли, стійкої до кили капустяних |
| BR112018000541B1 (pt) | 2015-08-18 | 2023-11-28 | Monsanto Technology Llc | Métodos para selecionar uma população de plantas ou sementes de algodão staygreen (stg), para selecionar uma planta ou semente de algodão e para avaliar uma coleção de germoplasma de algodão |
| US10448595B2 (en) | 2015-09-03 | 2019-10-22 | Seminis Vegetable Seeds, Inc. | Downy mildew resistant lettuce plants |
| US10858709B2 (en) | 2015-09-10 | 2020-12-08 | Monsanto Technology Llc | Methods for producing corn plants with downy mildew resistance and compositions thereof |
| WO2017106731A1 (en) | 2015-12-18 | 2017-06-22 | Monsanto Technology Llc | Methods for producing corn plants with northern leaf blight resistance and compositions thereof |
| US20190093172A1 (en) | 2016-04-15 | 2019-03-28 | Exosome Diagnostics, Inc. | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| CN109642229A (zh) | 2016-05-13 | 2019-04-16 | 外来体诊断公司 | 从生物液体分离细胞外囊泡和共分离无细胞dna的自动和手动方法 |
| EP3496528A4 (en) | 2016-08-11 | 2020-03-18 | Monsanto Technology LLC | METHODS AND COMPOSITIONS FOR PRODUCING CORN PLANTS WITH RESISTANCE TO LATE FAILURE |
| AU2017232187B2 (en) | 2016-09-30 | 2023-11-09 | Seminis Vegetable Seeds, Inc. | Xanthomonas resistant brassica oleracea plants |
| US11396676B2 (en) | 2016-10-21 | 2022-07-26 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| EP3548635B1 (en) | 2016-11-30 | 2021-04-28 | Exosome Diagnostics, Inc. | Methods to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients |
| US20200149036A1 (en) | 2017-01-02 | 2020-05-14 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| EP3652315A4 (en) | 2017-07-12 | 2021-09-01 | Exosome Diagnostics, Inc. | METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE |
| US11268102B2 (en) | 2018-05-16 | 2022-03-08 | University Of Florida Research Foundation, Incorporated | Compositions and methods for identifying and selecting brachytic locus in solanaceae |
| WO2020106853A1 (en) | 2018-11-20 | 2020-05-28 | Exosome Diagnostics, Inc. | Compositions and methods for internal controls of microvesicle isolations |
| AU2020208190B2 (en) | 2019-01-15 | 2022-07-28 | Seminis Vegetable Seeds, Inc. | Green bean plants with improved disease resistance |
| US11542513B2 (en) | 2019-09-26 | 2023-01-03 | Seminis Vegetable Seeds, Inc. | Lettuce plants having resistance to Nasonovia ribisnigri biotype Nr:1 |
| AU2021204717A1 (en) | 2020-07-15 | 2022-02-03 | Seminis Vegetable Seeds, Inc. | Green Bean Plants with Improved Disease Resistance |
| US20230193310A1 (en) | 2021-12-10 | 2023-06-22 | Seminis Vegetabe Seeds, Inc. | Lettuce plants having resistance to downy mildew |
| US20230404003A1 (en) | 2022-06-21 | 2023-12-21 | Seminis Vegetable Seeds, Inc. | Novel qtls conferring resistance to cucumber mosaic virus |
| MX2024004405A (es) | 2023-04-14 | 2024-11-08 | Seminis Vegetable Seeds Inc | Métodos y composiciones de resistencia a peronospora en la espinaca |
| US20260071285A1 (en) | 2024-09-11 | 2026-03-12 | Seminis Vegetable Seeds, Inc. | Novel qtls conferring resistance to cucurbit aphid-borne yellow virus |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981783A (en) | 1986-04-16 | 1991-01-01 | Montefiore Medical Center | Method for detecting pathological conditions |
| US6270961B1 (en) | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| US5925525A (en) | 1989-06-07 | 1999-07-20 | Affymetrix, Inc. | Method of identifying nucleotide differences |
| US5800992A (en) * | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| WO1993021345A1 (en) | 1992-04-21 | 1993-10-28 | The Regents Of The University Of California | Multicolor in situ hybridization methods for genetic testing |
| US5403708A (en) | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
| US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5834181A (en) | 1994-07-28 | 1998-11-10 | Genzyme Corporation | High throughput screening method for sequences or genetic alterations in nucleic acids |
| US5830645A (en) | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
| US5545531A (en) | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
| US5958342A (en) | 1996-05-17 | 1999-09-28 | Incyte Pharmaceuticals, Inc. | Jet droplet device |
| AU3568897A (en) | 1996-06-07 | 1998-01-05 | Eos Biotechnology, Inc. | Immobilised linear oligonucleotide arrays |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US6013449A (en) | 1997-11-26 | 2000-01-11 | The United States Of America As Represented By The Department Of Health And Human Services | Probe-based analysis of heterozygous mutations using two-color labelling |
| US6238869B1 (en) | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
| US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
| CA2306446A1 (en) | 1998-09-25 | 2000-04-06 | Massachusetts Institute Of Technology | Methods and products related to genotyping and dna analysis |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| ATE412774T1 (de) * | 2000-02-16 | 2008-11-15 | Illumina Inc | Parallele genotypisierung mehrerer patientenproben |
| US6913879B1 (en) * | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
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2000
- 2000-07-10 US US09/613,006 patent/US6913879B1/en not_active Expired - Fee Related
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2001
- 2001-07-03 EP EP01984136A patent/EP1343911B1/en not_active Expired - Lifetime
- 2001-07-03 CA CA2414105A patent/CA2414105C/en not_active Expired - Fee Related
- 2001-07-03 KR KR1020037000360A patent/KR100756015B1/ko not_active Expired - Fee Related
- 2001-07-03 AT AT01984136T patent/ATE363543T1/de not_active IP Right Cessation
- 2001-07-03 DE DE60128720T patent/DE60128720T2/de not_active Expired - Lifetime
- 2001-07-03 IL IL15384801A patent/IL153848A0/xx unknown
- 2001-07-03 JP JP2002508311A patent/JP4175884B2/ja not_active Expired - Fee Related
- 2001-07-03 CN CNB018139728A patent/CN100520401C/zh not_active Expired - Fee Related
- 2001-07-03 AU AU2002218740A patent/AU2002218740B2/en not_active Ceased
- 2001-07-03 RU RU2003103771/13A patent/RU2003103771A/ru not_active Application Discontinuation
- 2001-07-03 WO PCT/US2001/021163 patent/WO2002003849A2/en not_active Ceased
- 2001-07-03 NZ NZ523560A patent/NZ523560A/en unknown
- 2001-07-03 AU AU1874002A patent/AU1874002A/xx active Pending
- 2001-07-09 TW TW090116726A patent/TWI280282B/zh not_active IP Right Cessation
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- 2003-01-08 IL IL153848A patent/IL153848A/en not_active IP Right Cessation
- 2003-01-09 NO NO20030101A patent/NO20030101L/no not_active Application Discontinuation
- 2003-01-09 IS IS6674A patent/IS2651B/is unknown
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2004
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Also Published As
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| KR20030040352A (ko) | 2003-05-22 |
| ATE363543T1 (de) | 2007-06-15 |
| US20050153318A1 (en) | 2005-07-14 |
| RU2003103771A (ru) | 2004-08-20 |
| WO2002003849A2 (en) | 2002-01-17 |
| JP2004502441A (ja) | 2004-01-29 |
| NO20030101D0 (no) | 2003-01-09 |
| DE60128720D1 (de) | 2007-07-12 |
| AU2002218740B2 (en) | 2007-03-22 |
| NO20030101L (no) | 2003-03-07 |
| KR100756015B1 (ko) | 2007-09-07 |
| NZ523560A (en) | 2006-01-27 |
| EP1343911A2 (en) | 2003-09-17 |
| CA2414105A1 (en) | 2002-01-17 |
| CA2414105C (en) | 2011-07-12 |
| HK1057906A1 (en) | 2004-04-23 |
| IL153848A0 (en) | 2003-07-31 |
| IS6674A (is) | 2003-01-09 |
| IS2651B (is) | 2010-08-15 |
| AU1874002A (en) | 2002-01-21 |
| WO2002003849A3 (en) | 2003-06-19 |
| JP4175884B2 (ja) | 2008-11-05 |
| CN100520401C (zh) | 2009-07-29 |
| EP1343911B1 (en) | 2007-05-30 |
| IL153848A (en) | 2009-09-22 |
| US6913879B1 (en) | 2005-07-05 |
| CN1636137A (zh) | 2005-07-06 |
| TWI280282B (en) | 2007-05-01 |
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