DE60045180D1 - Klonierung und Verwendung einer neuen Nukleotidsequenz welche bakterielle Glukose-6-Phosphat Isomerase (GPI) kodiert - Google Patents

Klonierung und Verwendung einer neuen Nukleotidsequenz welche bakterielle Glukose-6-Phosphat Isomerase (GPI) kodiert

Info

Publication number
DE60045180D1
DE60045180D1 DE60045180T DE60045180T DE60045180D1 DE 60045180 D1 DE60045180 D1 DE 60045180D1 DE 60045180 T DE60045180 T DE 60045180T DE 60045180 T DE60045180 T DE 60045180T DE 60045180 D1 DE60045180 D1 DE 60045180D1
Authority
DE
Germany
Prior art keywords
polynucleotide
gpi
cloning
nucleotide sequence
sequence encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60045180T
Other languages
English (en)
Inventor
L K Prof Dunican
Ashling Mccormback
Cliona Stapelton
Kevin Burke
Michael O'donohue
Achim Dr Marx
Bettina Dr Moeckel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonik Operations GmbH
National University of Ireland
Original Assignee
Evonik Degussa GmbH
National University of Ireland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa GmbH, National University of Ireland filed Critical Evonik Degussa GmbH
Application granted granted Critical
Publication of DE60045180D1 publication Critical patent/DE60045180D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01009—Glucose-6-phosphate isomerase (5.3.1.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60045180T 1999-09-15 2000-08-23 Klonierung und Verwendung einer neuen Nukleotidsequenz welche bakterielle Glukose-6-Phosphat Isomerase (GPI) kodiert Expired - Lifetime DE60045180D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/396,478 US6586214B1 (en) 1999-09-15 1999-09-15 Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)

Publications (1)

Publication Number Publication Date
DE60045180D1 true DE60045180D1 (de) 2010-12-16

Family

ID=23567336

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60045180T Expired - Lifetime DE60045180D1 (de) 1999-09-15 2000-08-23 Klonierung und Verwendung einer neuen Nukleotidsequenz welche bakterielle Glukose-6-Phosphat Isomerase (GPI) kodiert

Country Status (16)

Country Link
US (1) US6586214B1 (de)
EP (1) EP1087015B1 (de)
JP (1) JP2001136988A (de)
KR (1) KR20010050449A (de)
CN (1) CN1288058A (de)
AT (1) ATE486942T1 (de)
AU (1) AU5505500A (de)
BR (1) BR0004208A (de)
CA (1) CA2318507A1 (de)
DE (1) DE60045180D1 (de)
HU (1) HUP0003651A3 (de)
ID (1) ID27238A (de)
MX (1) MXPA00008965A (de)
RU (1) RU2261912C2 (de)
SK (1) SK13362000A3 (de)
ZA (1) ZA200004911B (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070087089A (ko) * 1999-06-25 2007-08-27 바스프 악티엔게젤샤프트 탄소 대사 및 에너지 생산과 관련된 단백질을 코딩하는코리네박테리움 글루타미쿰 유전자
PT1208205E (pt) * 1999-07-23 2006-12-29 Archer Daniels Midland Co Métodos para produzir l-aminoácidos por aumento do nadph celular
US6586214B1 (en) * 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
US6818432B2 (en) 2000-01-13 2004-11-16 Degussa Ag Nucleotide sequences encoding the ptsH gene
US20050112733A1 (en) * 2000-03-20 2005-05-26 Degussa Ag Process for the preparation of L-amino acids with amplification of the zwf gene
US20030175911A1 (en) * 2000-03-20 2003-09-18 Stephen Hans Process for the preparation of L-amino acids with amplification of the zwf gene
EP1174520A3 (de) * 2000-07-18 2004-02-18 Degussa AG Verfahren zur Uberwachung einer Gärung mit hilfe eines expressions Array
US20030017554A1 (en) * 2000-11-15 2003-01-23 Mechthild Rieping Process for the fermentative preparation of L-amino acids using strains of the enterobacteriaceae family
ATE421577T1 (de) * 2001-04-04 2009-02-15 Genencor Int Entkoppelte anabole und katabole stoffwechselwege in wirtszellen
DE60229050D1 (de) 2001-04-04 2008-11-06 Genencor Int Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen
CA2462641A1 (en) * 2001-10-01 2003-04-10 Diversa Corporation Whole cell engineering using real-time metabolic flux analysis
DE10163167A1 (de) * 2001-12-21 2003-07-03 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren und Verwendung coryneformer Bakterien
DE10220574A1 (de) * 2002-05-08 2003-11-20 Degussa Verfahren zur Herstellung von Aminosäuren mit coryneformen Bakterien unter Verwendung von Phosphoglucose-Isomerasen aus coryneformen Bakterien
CN101230355A (zh) * 2003-12-18 2008-07-30 巴斯福股份公司 通过发酵制备精细化学品的方法
US20050181488A1 (en) * 2004-02-12 2005-08-18 Akhverdian Valery Z. Method for producing L-threonine using bacteria belonging to the genus Escherichia
DE102005013676A1 (de) 2005-03-24 2006-09-28 Degussa Ag Allele des zwf-Gens aus coryneformen Bakterien
US20070092951A1 (en) 2005-03-24 2007-04-26 Degussa Ag Alleles of the zwf gene from coryneform bacteria
DE102005023829A1 (de) 2005-05-24 2006-11-30 Degussa Ag Allele des opcA-Gens aus coryneformen Bakterien
RU2396336C2 (ru) 2007-09-27 2010-08-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia
KR101049023B1 (ko) 2008-03-05 2011-07-12 씨제이제일제당 (주) 5'-이노신산 생산능이 향상된 코리네박테리움암모니아게네스 및 그를 이용한 5'-이노신산의 생산방법
US7821360B2 (en) * 2008-08-28 2010-10-26 Northrop Grumman Systems Corporation Multi-channel surface acoustic wave filter device with voltage controlled tunable frequency response
KR101166027B1 (ko) * 2009-04-01 2012-07-19 씨제이제일제당 (주) 5'-이노신산 생산성이 향상된 코리네박테리움 속 미생물 및 이를 이용한 핵산의 생산방법
DE102011006716A1 (de) * 2011-04-04 2012-10-04 Evonik Degussa Gmbh Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung
CN104845923B (zh) 2014-02-14 2018-03-23 中国科学院微生物研究所 生产l‑组氨酸的方法及其专用重组菌
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US11293029B2 (en) 2015-12-07 2022-04-05 Zymergen Inc. Promoters from Corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR102345899B1 (ko) 2016-06-30 2021-12-31 지머젠 인코포레이티드 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
KR102345898B1 (ko) 2016-06-30 2022-01-03 지머젠 인코포레이티드 글루코오스 투과 효소 라이브러리를 생성하는 방법 및 이의 용도
CA3064777A1 (en) 2017-06-07 2018-12-13 Zach Serber Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
CN109423504B (zh) * 2017-08-24 2022-10-04 廊坊梅花生物技术开发有限公司 生产l-色氨酸的菌株及用途
SG11202100450TA (en) * 2018-07-16 2021-02-25 Manus Bio Inc Production of steviol glycosides through whole cell biotransformation

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58126789A (ja) 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd レースレオニンの製造法
JP3369231B2 (ja) * 1992-12-03 2003-01-20 協和醗酵工業株式会社 芳香族アミノ酸の製造法
KR20070087089A (ko) 1999-06-25 2007-08-27 바스프 악티엔게젤샤프트 탄소 대사 및 에너지 생산과 관련된 단백질을 코딩하는코리네박테리움 글루타미쿰 유전자
PT1208205E (pt) 1999-07-23 2006-12-29 Archer Daniels Midland Co Métodos para produzir l-aminoácidos por aumento do nadph celular
US6586214B1 (en) * 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
JP4623825B2 (ja) 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
CN101029310B (zh) * 2000-03-20 2010-12-08 赢创德固赛有限公司 经扩增zwf基因发酵制备L-氨基酸的方法

Also Published As

Publication number Publication date
EP1087015B1 (de) 2010-11-03
HUP0003651A3 (en) 2005-07-28
HUP0003651A2 (hu) 2003-04-28
CA2318507A1 (en) 2001-03-15
SK13362000A3 (sk) 2003-01-09
EP1087015A3 (de) 2003-07-09
MXPA00008965A (es) 2002-12-16
BR0004208A (pt) 2001-04-10
CN1288058A (zh) 2001-03-21
ID27238A (id) 2001-03-15
AU5505500A (en) 2001-03-22
US6586214B1 (en) 2003-07-01
EP1087015A2 (de) 2001-03-28
RU2261912C2 (ru) 2005-10-10
ATE486942T1 (de) 2010-11-15
ZA200004911B (en) 2002-03-13
HU0003651D0 (en) 2000-09-14
KR20010050449A (ko) 2001-06-15
JP2001136988A (ja) 2001-05-22

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