ATE132529T1 - Klonierung von methylierter dns in einem bakteriellen wirtsstamm der zur wirtsrestriktion unfähig ist - Google Patents

Klonierung von methylierter dns in einem bakteriellen wirtsstamm der zur wirtsrestriktion unfähig ist

Info

Publication number
ATE132529T1
ATE132529T1 AT93200367T AT93200367T ATE132529T1 AT E132529 T1 ATE132529 T1 AT E132529T1 AT 93200367 T AT93200367 T AT 93200367T AT 93200367 T AT93200367 T AT 93200367T AT E132529 T1 ATE132529 T1 AT E132529T1
Authority
AT
Austria
Prior art keywords
cloning
host
inapable
restriction
methylated dna
Prior art date
Application number
AT93200367T
Other languages
English (en)
Inventor
Jan Vijg
Jan Albert Gossen
Original Assignee
Tno
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19852648&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE132529(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Tno filed Critical Tno
Application granted granted Critical
Publication of ATE132529T1 publication Critical patent/ATE132529T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT93200367T 1988-07-19 1989-07-18 Klonierung von methylierter dns in einem bakteriellen wirtsstamm der zur wirtsrestriktion unfähig ist ATE132529T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8801826A NL8801826A (nl) 1988-07-19 1988-07-19 Werkwijze voor het detecteren van mutaties in markergenen.

Publications (1)

Publication Number Publication Date
ATE132529T1 true ATE132529T1 (de) 1996-01-15

Family

ID=19852648

Family Applications (2)

Application Number Title Priority Date Filing Date
AT89201895T ATE100151T1 (de) 1988-07-19 1989-07-18 Verfahren fuer die rettung von dns und fuer den nachweis von mutationen in markiergenen.
AT93200367T ATE132529T1 (de) 1988-07-19 1989-07-18 Klonierung von methylierter dns in einem bakteriellen wirtsstamm der zur wirtsrestriktion unfähig ist

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT89201895T ATE100151T1 (de) 1988-07-19 1989-07-18 Verfahren fuer die rettung von dns und fuer den nachweis von mutationen in markiergenen.

Country Status (8)

Country Link
US (1) US5470706A (de)
EP (3) EP0353812B1 (de)
JP (1) JPH0829093B2 (de)
AT (2) ATE100151T1 (de)
DE (2) DE68912228T2 (de)
ES (2) ES2081679T3 (de)
GR (1) GR3018927T3 (de)
NL (1) NL8801826A (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9100567A (nl) * 1991-04-02 1992-11-02 Ingeny Bv Werkwijze voor het detecteren van mutaties, transgeen zoogdier, transgene zoogdiercel, en werkwijze voor het onderzoeken van stoffen of omstandigheden op mutagene eigenschappen.
CA2130081A1 (en) * 1992-02-14 1993-08-19 Jay M. Short Mutagenesis testing using transgenic non-human animals carrying test dna sequences
AU4220093A (en) * 1992-04-27 1993-11-29 Indiana University Foundation Live animal mutagenesis systems for testing mutagenic agents in vivo
ES2111289T5 (es) * 1993-01-21 2005-07-16 President And Fellows Of Harvard College Metodos y kits de diagnostico que emplean promotores de estres en mamiferos para determinar la toxicidad de un compuesto.
ATE301390T1 (de) 1998-05-31 2005-08-15 Univ Georgia Res Found Transgene fische, die ein von bakteriophagen abgeleitetes transgenes konstrukt zum nachweis von mutationen tragen
AU1323000A (en) 1998-10-26 2000-05-15 University Of Georgia Research Foundation, Inc., The Transgenic fish carrying plasmid for mutation detection and methods
GB9924973D0 (en) * 1999-10-21 1999-12-22 Cobra Therapeutics Ltd Preparation of cellular components that are substantially free of chromosomal DNA
KR20040082273A (ko) * 2001-05-21 2004-09-24 아클라라 바이오사이언시스 인코퍼레이티드 단백질 분석을 위한 방법 및 조성물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4753874A (en) * 1985-07-08 1988-06-28 Board Of Trustees Of The Leland Stanford Junior University Rapid mutation testing system for human cells
DE3854823T2 (de) * 1987-05-01 1996-05-23 Stratagene Inc Mutagenesetest durch Verwendung von nicht menschlichen Lebewesen, die Test-DNS-Sequenzen enthalten

Also Published As

Publication number Publication date
ES2081679T3 (es) 1996-03-16
DE68925369T2 (de) 1996-05-15
DE68912228D1 (de) 1994-02-24
ATE100151T1 (de) 1994-01-15
DE68912228T2 (de) 1994-08-04
EP0548056B1 (de) 1996-01-03
EP0548056A1 (de) 1993-06-23
US5470706A (en) 1995-11-28
EP0353812A2 (de) 1990-02-07
EP0548056B2 (de) 2001-04-04
EP0683238A3 (de) 1995-12-20
NL8801826A (nl) 1990-02-16
JPH0829093B2 (ja) 1996-03-27
EP0353812A3 (en) 1990-03-07
JPH02142471A (ja) 1990-05-31
DE68925369D1 (de) 1996-02-15
EP0683238A2 (de) 1995-11-22
ES2061943T3 (es) 1994-12-16
GR3018927T3 (en) 1996-05-31
EP0353812B1 (de) 1994-01-12
DE68925369T3 (de) 2001-08-09

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