DE50204952D1 - Verfahren zur herstellung von rekombinantem trypsin - Google Patents

Verfahren zur herstellung von rekombinantem trypsin

Info

Publication number
DE50204952D1
DE50204952D1 DE50204952T DE50204952T DE50204952D1 DE 50204952 D1 DE50204952 D1 DE 50204952D1 DE 50204952 T DE50204952 T DE 50204952T DE 50204952 T DE50204952 T DE 50204952T DE 50204952 D1 DE50204952 D1 DE 50204952D1
Authority
DE
Germany
Prior art keywords
trypsin
recombinant
trypsine
preparation
trypsinogen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE50204952T
Other languages
German (de)
English (en)
Inventor
Rainer Mueller
Stephan Glaser
Frank Geipel
Johann-Peter Thalhofer
Bernhard Rexer
Claus Schneider
Michael Ratka
Stephanie Ronning
Hellmut Eckstein
Claudia Giessel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8176376&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE50204952(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE50204952D1 publication Critical patent/DE50204952D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE50204952T 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin Expired - Lifetime DE50204952D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01102342 2001-02-01
PCT/EP2002/001072 WO2002061064A2 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Publications (1)

Publication Number Publication Date
DE50204952D1 true DE50204952D1 (de) 2005-12-22

Family

ID=8176376

Family Applications (1)

Application Number Title Priority Date Filing Date
DE50204952T Expired - Lifetime DE50204952D1 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Country Status (10)

Country Link
US (2) US7276605B2 (https=)
EP (1) EP1399568B1 (https=)
JP (1) JP4159878B2 (https=)
CN (1) CN1545553B (https=)
AT (1) ATE310092T1 (https=)
CA (1) CA2437342C (https=)
CZ (1) CZ303658B6 (https=)
DE (1) DE50204952D1 (https=)
DK (1) DK1399568T3 (https=)
WO (1) WO2002061064A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin
CN1694953B (zh) * 2002-08-30 2010-05-12 诺维信股份有限公司 制备哺乳动物胰蛋白酶的方法
AU2006291780B2 (en) * 2005-09-14 2011-12-08 F. Hoffmann-La Roche Ag Cleavage of precursors of insulins by a variant of trypsin
CA2746470A1 (en) * 2009-01-06 2010-07-15 Raymond-David Pridmore Processing of macronutrients
CN101967469B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组胰蛋白酶的生产及应用
US8975041B2 (en) 2009-09-10 2015-03-10 Biocon Limited Fusion proteins and method of expression thereof
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103805584B (zh) * 2012-11-12 2016-08-24 宜昌东阳光长江药业股份有限公司 一种重组胰蛋白酶纯化方法
CN103405756B (zh) * 2013-07-10 2014-12-24 浙江众益制药股份有限公司 药物组合物复方消化酶胶囊(ii)及其制备方法
EP3797780B1 (en) 2014-04-17 2022-09-14 Biogen MA Inc. Compositions and methods for modulation of smn2 splicing in a subject
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
WO2016164896A2 (en) 2015-04-10 2016-10-13 Ionis Pharmaceuticals, Inc. Modulation of smn expression
US11198867B2 (en) 2016-06-16 2021-12-14 Ionis Pharmaceuticals, Inc. Combinations for the modulation of SMN expression
CN106350497B (zh) * 2016-11-24 2019-07-16 天津大学 一种胰蛋白酶及其制备方法与应用
CN110511916B (zh) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 一种重组胰蛋白酶的生产工艺
CN120505310A (zh) 2020-02-28 2025-08-19 Ionis制药公司 用于调节smn2的化合物和方法
CN115216463B (zh) * 2022-06-15 2023-08-15 武汉瀚海新酶生物科技有限公司 具有稳定性的重组胰蛋白酶及其制备方法和应用
WO2024110426A1 (en) 2022-11-23 2024-05-30 F. Hoffmann-La Roche Ag Method for increasing recombinant protein expression

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE249282C (https=)
US5011912A (en) * 1986-12-19 1991-04-30 Immunex Corporation Hybridoma and monoclonal antibody for use in an immunoaffinity purification system
US4851341A (en) * 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
US7247453B1 (en) * 1988-12-30 2007-07-24 Oklahoma Medical Research Foundation Calcium binding recombinant antibody against protein C
US5298599A (en) * 1988-12-30 1994-03-29 Oklahoma Medical Research Foundation Expression and purification of recombinant soluble tissue factor
US6433142B1 (en) * 1989-08-08 2002-08-13 Genetics Institute, Llc Megakaryocyte stimulating factors
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
CA2102673A1 (en) * 1992-11-13 1994-05-14 Michael G. Greaney Expression vectors for bovine trypsin and trypsinogen and host cells transformed therewith
US5472692A (en) * 1993-07-02 1995-12-05 New England Deaconess Hospital Corporation Pro-urokinase mutants
DK0699687T3 (da) 1994-08-31 2004-04-26 Mitsubishi Pharma Corp Fremgangsmåde til oprensning af rekombinant humant serumalbumin
US5760189A (en) * 1995-06-02 1998-06-02 Genetics Institute, Inc. Protein recovery & purification methods
WO1997000316A1 (en) 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
US5945328A (en) 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
CN1187849A (zh) * 1995-06-16 1998-07-15 诺沃挪第克公司 产生胰蛋白酶(胰蛋白酶原)的方法
CA2252439C (en) * 1996-04-19 2014-09-30 Henry M. Jackson Foundation For The Advancement Of Military Medicine Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens
GB9609702D0 (en) * 1996-05-09 1996-07-10 Royal Free Hosp School Med Anticoagulant peptides
US6387638B1 (en) * 1997-02-10 2002-05-14 Genentech, Inc. Heregulin variants
DE59812768D1 (de) 1997-08-22 2005-06-09 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
US20030207402A1 (en) 1997-08-22 2003-11-06 Erhard Kopetzki Autocatalytically activatable zymogenic precursors of proteases and their use
US6159722A (en) * 1997-12-03 2000-12-12 Boehringer Mannheim Gmbh Chimeric serine proteases
US6486303B1 (en) * 1998-04-14 2002-11-26 University Of Medicine & Dentistry Of New Jersey Method for making hormone heterodimers
US6087558A (en) * 1998-07-22 2000-07-11 Prodigene, Inc. Commercial production of proteases in plants
WO2000017332A1 (en) 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
BR9915548A (pt) * 1998-10-16 2001-08-14 Biogen Inc Proteìnas de fusão de interferon-beta e usos
JP4475814B2 (ja) * 1998-11-20 2010-06-09 扶桑薬品工業株式会社 タンパク質発現ベクターとその使用
US20020102709A1 (en) * 1999-02-19 2002-08-01 Tetsuya Ishikawa Collagen-binding physiologically active polypeptide
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6485957B1 (en) * 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6458564B1 (en) * 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
WO2001019970A2 (en) * 1999-09-15 2001-03-22 Eli Lilly And Company Chymotrypsin-free trypsin
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
WO2001055429A2 (en) 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
WO2001098366A2 (en) * 2000-06-19 2001-12-27 Dyax Corp. Enterokinase cleavage sequences and their use
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
US6831059B2 (en) * 2000-10-20 2004-12-14 Allergan, Inc. Compositions and methods for treating gonadotrophin related illnesses
US6632638B1 (en) * 2000-11-17 2003-10-14 Amgen, Inc. Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
AU2002233230B2 (en) * 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin
AU2002254357A1 (en) * 2001-03-22 2002-10-08 Dendreon Corporation Nucleic acid molecules encoding serine protease cvsp14, the encoded polypeptides and methods based thereon

Also Published As

Publication number Publication date
CA2437342A1 (en) 2002-08-08
CA2437342C (en) 2013-08-06
CZ303658B6 (cs) 2013-02-06
US7276605B2 (en) 2007-10-02
CN1545553B (zh) 2011-09-21
JP4159878B2 (ja) 2008-10-01
CZ20032337A3 (cs) 2004-04-14
ATE310092T1 (de) 2005-12-15
US20040203095A1 (en) 2004-10-14
EP1399568A2 (de) 2004-03-24
CN1545553A (zh) 2004-11-10
US7666629B2 (en) 2010-02-23
WO2002061064A3 (de) 2003-12-24
EP1399568B1 (de) 2005-11-16
DK1399568T3 (da) 2006-03-27
WO2002061064A2 (de) 2002-08-08
JP2004520049A (ja) 2004-07-08
US20080064084A1 (en) 2008-03-13

Similar Documents

Publication Publication Date Title
DE50204952D1 (de) Verfahren zur herstellung von rekombinantem trypsin
ATE236537T1 (de) Verfahren zur erzeugung von proteinhydrolysaten
UA95438C2 (ru) Биспецифические антитела, которые замещают функциональные белки
DE69432744D1 (de) Verbessertes verfahren zur rückfaltung der proteine
DE60018909D1 (de) Neue carbonyl-reduktase, gen davon und verfahren der anwendung derselben
ATE124456T1 (de) Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen.
ATE350470T1 (de) Durch thrombin spaltbare chimäre proteine
ATE474847T1 (de) Verfahren zur herstellung von dipeptiden
DE60125218D1 (de) Verfahren zur herstellung von humanem serumalbumin unter verwendung eines aufwarmschrittes
DE60210298D1 (de) Verfahren zur herstellung von polynucleotivarianten
ATE341640T1 (de) Verfahren zur herstellung von nicht-proteinogenen l-aminosäuren
DE69331371D1 (de) Dipeptidylaminopeptidase von Dictyostelium
ATE365709T1 (de) Verfahren zur herstellung von pyrazinverbindungen
ATE227307T1 (de) Verfahren zur herstellung modifizierten proteine
DE59309646D1 (de) Verfahren zur Spaltung von Proenzymen
DE60105753D1 (de) Verfahren zur Herstellung eines glutenfreien Peptidepräparates sowie das dabei erhaltene Präparat
DE60038281D1 (de) Verfahren zur herstellung von optisch aktiven pyridinethanol-derivaten
ATE319837T1 (de) Verfahren zur herstellung von rekombinantem trypsin
DE60040771D1 (de) Verfahren zur kontrolle der spaltung mit ompt protease
RU2003109412A (ru) Способ получения белкового гидролизата из твердых хромосодержащих отходов кожевенного производства
DE50001961D1 (de) Verfahren zur herstellung von eiweisshydrolysaten
DE50105715D1 (de) Verfahren zur herstellung von dnda
DE50111910D1 (de) Verfahren zur synthese von peptiden, peptidmimetika und proteinen
DE59106487D1 (de) Verfahren zur herstellung von peptiden.
DE69108192D1 (de) Verfahren zur enzymatischen Herstellung von GRF(1-44)NH2.