CN1545553B - 生产重组胰蛋白酶的方法 - Google Patents

生产重组胰蛋白酶的方法 Download PDF

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Publication number
CN1545553B
CN1545553B CN02805619.1A CN02805619A CN1545553B CN 1545553 B CN1545553 B CN 1545553B CN 02805619 A CN02805619 A CN 02805619A CN 1545553 B CN1545553 B CN 1545553B
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China
Prior art keywords
nucleic acid
recombinant
trypsinogen
expression
recombinant nucleic
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Expired - Lifetime
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CN02805619.1A
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English (en)
Chinese (zh)
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CN1545553A (zh
Inventor
R·米勒
S·格莱泽
F·盖佩尔
J-P·塔尔霍费尔
B·雷克塞尔
C·施奈德
M·拉特卡
S·伦宁
H·埃克施泰因
C·吉塞尔
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN02805619.1A 2001-02-01 2002-02-01 生产重组胰蛋白酶的方法 Expired - Lifetime CN1545553B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01102342 2001-02-01
EP01102342.1 2001-02-01
PCT/EP2002/001072 WO2002061064A2 (de) 2001-02-01 2002-02-01 Verfahren zur herstellung von rekombinantem trypsin

Publications (2)

Publication Number Publication Date
CN1545553A CN1545553A (zh) 2004-11-10
CN1545553B true CN1545553B (zh) 2011-09-21

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CN02805619.1A Expired - Lifetime CN1545553B (zh) 2001-02-01 2002-02-01 生产重组胰蛋白酶的方法

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US (2) US7276605B2 (https=)
EP (1) EP1399568B1 (https=)
JP (1) JP4159878B2 (https=)
CN (1) CN1545553B (https=)
AT (1) ATE310092T1 (https=)
CA (1) CA2437342C (https=)
CZ (1) CZ303658B6 (https=)
DE (1) DE50204952D1 (https=)
DK (1) DK1399568T3 (https=)
WO (1) WO2002061064A2 (https=)

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CN1694953B (zh) * 2002-08-30 2010-05-12 诺维信股份有限公司 制备哺乳动物胰蛋白酶的方法
AU2006291780B2 (en) * 2005-09-14 2011-12-08 F. Hoffmann-La Roche Ag Cleavage of precursors of insulins by a variant of trypsin
CA2746470A1 (en) * 2009-01-06 2010-07-15 Raymond-David Pridmore Processing of macronutrients
CN101967469B (zh) * 2009-07-28 2012-11-14 上海雅心生物技术有限公司 一种高稳定性的重组胰蛋白酶的生产及应用
US8975041B2 (en) 2009-09-10 2015-03-10 Biocon Limited Fusion proteins and method of expression thereof
WO2012104099A1 (en) 2011-02-04 2012-08-09 Glucometrix Ag Process for the production of recombinant trypsin
CN103805584B (zh) * 2012-11-12 2016-08-24 宜昌东阳光长江药业股份有限公司 一种重组胰蛋白酶纯化方法
CN103405756B (zh) * 2013-07-10 2014-12-24 浙江众益制药股份有限公司 药物组合物复方消化酶胶囊(ii)及其制备方法
EP3797780B1 (en) 2014-04-17 2022-09-14 Biogen MA Inc. Compositions and methods for modulation of smn2 splicing in a subject
CN104046605A (zh) * 2014-05-29 2014-09-17 中国科学院广州能源研究所 一种嗜温耐乙醇β-葡萄糖苷酶及其编码基因和应用
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
WO2016164896A2 (en) 2015-04-10 2016-10-13 Ionis Pharmaceuticals, Inc. Modulation of smn expression
US11198867B2 (en) 2016-06-16 2021-12-14 Ionis Pharmaceuticals, Inc. Combinations for the modulation of SMN expression
CN106350497B (zh) * 2016-11-24 2019-07-16 天津大学 一种胰蛋白酶及其制备方法与应用
CN110511916B (zh) * 2019-08-06 2021-06-01 杭州浦泰生物科技有限公司 一种重组胰蛋白酶的生产工艺
CN120505310A (zh) 2020-02-28 2025-08-19 Ionis制药公司 用于调节smn2的化合物和方法
CN115216463B (zh) * 2022-06-15 2023-08-15 武汉瀚海新酶生物科技有限公司 具有稳定性的重组胰蛋白酶及其制备方法和应用
WO2024110426A1 (en) 2022-11-23 2024-05-30 F. Hoffmann-La Roche Ag Method for increasing recombinant protein expression

Citations (1)

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CN1187849A (zh) * 1995-06-16 1998-07-15 诺沃挪第克公司 产生胰蛋白酶(胰蛋白酶原)的方法

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Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1187849A (zh) * 1995-06-16 1998-07-15 诺沃挪第克公司 产生胰蛋白酶(胰蛋白酶原)的方法

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CA2437342A1 (en) 2002-08-08
CA2437342C (en) 2013-08-06
CZ303658B6 (cs) 2013-02-06
US7276605B2 (en) 2007-10-02
JP4159878B2 (ja) 2008-10-01
CZ20032337A3 (cs) 2004-04-14
ATE310092T1 (de) 2005-12-15
US20040203095A1 (en) 2004-10-14
EP1399568A2 (de) 2004-03-24
CN1545553A (zh) 2004-11-10
US7666629B2 (en) 2010-02-23
WO2002061064A3 (de) 2003-12-24
EP1399568B1 (de) 2005-11-16
DK1399568T3 (da) 2006-03-27
DE50204952D1 (de) 2005-12-22
WO2002061064A2 (de) 2002-08-08
JP2004520049A (ja) 2004-07-08
US20080064084A1 (en) 2008-03-13

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Granted publication date: 20110921