DE60210298D1 - Verfahren zur herstellung von polynucleotivarianten - Google Patents

Verfahren zur herstellung von polynucleotivarianten

Info

Publication number
DE60210298D1
DE60210298D1 DE60210298T DE60210298T DE60210298D1 DE 60210298 D1 DE60210298 D1 DE 60210298D1 DE 60210298 T DE60210298 T DE 60210298T DE 60210298 T DE60210298 T DE 60210298T DE 60210298 D1 DE60210298 D1 DE 60210298D1
Authority
DE
Germany
Prior art keywords
polynucleotivariants
producing
polynucleotides
preparation
discloses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60210298T
Other languages
English (en)
Other versions
DE60210298T2 (de
Inventor
Ary Bovenberg
Richard Kerkman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of DE60210298D1 publication Critical patent/DE60210298D1/de
Application granted granted Critical
Publication of DE60210298T2 publication Critical patent/DE60210298T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
DE60210298T 2001-07-23 2002-07-23 Verfahren zur herstellung von polynucleotidvarianten Expired - Lifetime DE60210298T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP01202822 2001-07-23
EP01202822 2001-07-23
EP01203458 2001-09-11
EP01203458 2001-09-11
PCT/EP2002/008222 WO2003010311A2 (en) 2001-07-23 2002-07-23 Process for preparing variant polynucleotides

Publications (2)

Publication Number Publication Date
DE60210298D1 true DE60210298D1 (de) 2006-05-18
DE60210298T2 DE60210298T2 (de) 2006-11-02

Family

ID=26076966

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60210298T Expired - Lifetime DE60210298T2 (de) 2001-07-23 2002-07-23 Verfahren zur herstellung von polynucleotidvarianten

Country Status (5)

Country Link
US (1) US20050130140A1 (de)
EP (1) EP1409666B1 (de)
AT (1) ATE321850T1 (de)
DE (1) DE60210298T2 (de)
WO (1) WO2003010311A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080146965A1 (en) 2003-08-11 2008-06-19 Salvatore Privitera Surgical Device for The Collection of Soft Tissue
US20010047183A1 (en) 2000-04-05 2001-11-29 Salvatore Privitera Surgical device for the collection of soft tissue
US7402383B2 (en) * 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
KR100803093B1 (ko) * 2005-10-07 2008-02-18 한국해양연구원 광학선택적 에폭사이드 가수분해효소 및 이를 이용한광학순도 에폭사이드의 제조방법
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
ES2548400T3 (es) 2010-11-12 2015-10-16 Gen9, Inc. Métodos y dispositivos para la síntesis de ácidos nucleicos
EP2944693B1 (de) 2011-08-26 2019-04-24 Gen9, Inc. Zusammensetzungen und verfahren zur hochqualitativen anordnung von nukleinsäuren
AU2013211605B2 (en) 2012-01-23 2017-04-06 Dsm Ip Assets B.V. Diterpene production
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP4001427A1 (de) 2012-04-24 2022-05-25 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
JP6509727B2 (ja) 2012-06-25 2019-05-15 ギンゴー バイオワークス， インコーポレイテッド 核酸アセンブリおよび高処理シークエンシングのための方法
DK3004366T3 (da) 2013-05-31 2019-05-20 Dsm Ip Assets Bv Mikroorganismer til diterpenproduktion
BR112016000745B1 (pt) 2013-07-15 2021-01-05 Dsm Ip Assets B.V. processo para a preparação de rebaudiosídeo m
EP3536697A1 (de) 2013-07-31 2019-09-11 DSM IP Assets B.V. Rückgewinnung von steviolglycosiden
CA3198626A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6537746B2 (en) * 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
FR2782323B1 (fr) * 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US20050003354A1 (en) * 2001-02-21 2005-01-06 Maxygen, Inc. Methods for improving or altering promoter/enhancer properties
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics

Also Published As

Publication number Publication date
WO2003010311A2 (en) 2003-02-06
US20050130140A1 (en) 2005-06-16
EP1409666B1 (de) 2006-03-29
EP1409666A2 (de) 2004-04-21
ATE321850T1 (de) 2006-04-15
DE60210298T2 (de) 2006-11-02
WO2003010311A3 (en) 2003-12-11

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