DE3473298D1 - Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins - Google Patents
Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulinsInfo
- Publication number
- DE3473298D1 DE3473298D1 DE8484900773T DE3473298T DE3473298D1 DE 3473298 D1 DE3473298 D1 DE 3473298D1 DE 8484900773 T DE8484900773 T DE 8484900773T DE 3473298 T DE3473298 T DE 3473298T DE 3473298 D1 DE3473298 D1 DE 3473298D1
- Authority
- DE
- Germany
- Prior art keywords
- immunoglobulins
- polypeptides
- recombinant
- bind
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108060003951 Immunoglobulin Proteins 0.000 title 1
- 239000013604 expression vector Substances 0.000 title 1
- 108020001507 fusion proteins Proteins 0.000 title 1
- 102000037865 fusion proteins Human genes 0.000 title 1
- 102000018358 immunoglobulin Human genes 0.000 title 1
- 229940072221 immunoglobulins Drugs 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE8300693A SE8300693L (sv) | 1983-02-09 | 1983-02-09 | Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta |
PCT/SE1984/000046 WO1984003103A1 (en) | 1983-02-09 | 1984-02-09 | Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins |
Publications (1)
Publication Number | Publication Date |
---|---|
DE3473298D1 true DE3473298D1 (en) | 1988-09-15 |
Family
ID=20349966
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE8484900773T Expired DE3473298D1 (en) | 1983-02-09 | 1984-02-09 | Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins |
Country Status (6)
Country | Link |
---|---|
US (1) | US5100788A (de) |
EP (1) | EP0135532B1 (de) |
JP (2) | JPH0634742B2 (de) |
DE (1) | DE3473298D1 (de) |
SE (1) | SE8300693L (de) |
WO (1) | WO1984003103A1 (de) |
Families Citing this family (66)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE95236T1 (de) | 1983-04-25 | 1993-10-15 | Chiron Corp | Hybrid-dns-synthesis von reifen insulinaehnlichen wachstumsfaktoren. |
IL71991A (en) | 1983-06-06 | 1994-05-30 | Genentech Inc | Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes |
WO1985000831A1 (en) * | 1983-08-10 | 1985-02-28 | Amgen | Microbial expression of insulin-like growth factor |
US4708929A (en) * | 1984-10-29 | 1987-11-24 | Microgenics Corporation | Methods for protein binding enzyme complementation assays |
DK108685A (da) * | 1984-03-19 | 1985-09-20 | Fujisawa Pharmaceutical Co | Vaekstfaktor i |
US5248666A (en) * | 1984-03-23 | 1993-09-28 | Oncogen | Methods for inhibiting neoplastic cell proliferation using platelet factor 4 |
US4745179A (en) * | 1984-04-02 | 1988-05-17 | Fujisawa Pharmaceutical Co., Ltd. | 59 Valine insulin-like growth factor I and process for production thereof |
GB8412517D0 (en) * | 1984-05-16 | 1984-06-20 | Nagai K | Recombinant fusion proteins |
US5028530A (en) * | 1985-01-28 | 1991-07-02 | Xoma Corporation | AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques |
JPS61264000A (ja) * | 1985-03-21 | 1986-11-21 | イミユネツクス コ−ポレイシヨン | 標識ペプチドによるタンパク質の合成 |
GB8507666D0 (en) * | 1985-03-25 | 1985-05-01 | Wellcome Found | Epidermal growth factor production |
CA1260858A (en) * | 1985-03-28 | 1989-09-26 | Lawrence S. Cousens | Expression using fused genes providing for protein product |
US5342921A (en) * | 1985-03-28 | 1994-08-30 | Chiron Corporation | Superoxide dismutase fusion polypeptides for expression of mammalian proteins |
US4751180A (en) * | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
US5169939A (en) * | 1985-05-21 | 1992-12-08 | Massachusetts Institute Of Technology & Pres. & Fellows Of Harvard College | Chimeric antibodies |
SE8505921D0 (sv) * | 1985-12-13 | 1985-12-13 | Kabigen Ab | A method to export gene products to the growth medium of gram negative bacteria |
SE8505922D0 (sv) * | 1985-12-13 | 1985-12-13 | Kabigen Ab | Construction of an igg binding protein to facilitate downstream processing using protein engineering |
DE3545568A1 (de) * | 1985-12-21 | 1987-07-16 | Hoechst Ag | Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung |
SE459662B (sv) * | 1986-03-21 | 1989-07-24 | Pharmacia Ab | Hybrid-dna-molekyl som kodar foer ett protein med samma igg specificitet som protein g, plasmid innehaallande densamma samt foerfarande foer framstaellning av proteinet |
SE8601940D0 (sv) * | 1986-04-25 | 1986-04-25 | Kabigen Ab | Preparation of fused proteins, antibodies and processes therefor |
AU623589B2 (en) * | 1987-03-02 | 1992-05-21 | Bristol-Myers Squibb Company | Platelet related growth regulator |
DK175535B1 (da) * | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid |
US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
US5013653A (en) * | 1987-03-20 | 1991-05-07 | Creative Biomolecules, Inc. | Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage |
US5215896A (en) * | 1987-03-20 | 1993-06-01 | Creative Biomolecules, Inc. | Leader sequences for the production of recombinant proteins |
US5095096A (en) * | 1987-05-29 | 1992-03-10 | Sagami Chemical Research Center | Fused protein comprising lymphotoxin |
US5212064A (en) * | 1987-09-21 | 1993-05-18 | Microgenics Corporation | Solid phase non-separation enzyme complementation assay |
US5243040A (en) * | 1987-11-20 | 1993-09-07 | Creative Biomolecules | DNA encoding a protein which enables selective removal of immune complexes |
US5084398A (en) * | 1987-11-20 | 1992-01-28 | Creative Biomolecules | Selective removal of immune complexes |
SE501169C2 (sv) * | 1988-02-05 | 1994-11-28 | Aake Nygren | Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner |
SE8800981A (sv) * | 1988-03-17 | 1988-10-14 | Lars Abrahamsen | A recombinant fusion protein, its use and a recombinant vector |
CA2005252C (en) * | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolation of full-length cdna clones and capped mrna |
US6267964B1 (en) * | 1989-08-01 | 2001-07-31 | Affibody Technology Sweden Ab | Stabilized protein or peptide conjugates able to bond albumin having extended biological half-lives |
US5620923A (en) * | 1989-10-12 | 1997-04-15 | The University Of Utah | Synthesis of peptides as cloned ubiquitin extensions |
US5624822A (en) * | 1989-12-22 | 1997-04-29 | Basf Aktiengesellschaft | Hirudin fusion proteins and preparation of hirudin |
DE3942580A1 (de) * | 1989-12-22 | 1991-06-27 | Basf Ag | Verfahren zur herstellung von hirudin |
JPH0740924B2 (ja) * | 1990-08-22 | 1995-05-10 | エム・ディ・リサーチ株式会社 | 形質転換体および融合蛋白質の製造方法 |
US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
CA2090969C (en) * | 1990-09-04 | 2001-08-21 | Russell Arthur Brierley | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
JPH0740926B2 (ja) * | 1990-10-09 | 1995-05-10 | エム・ディ・リサーチ株式会社 | 形質転換体およびペプチド前駆体タンパク質の製造方法 |
JPH07108232B2 (ja) * | 1990-10-09 | 1995-11-22 | エム・ディ・リサーチ株式会社 | ペプチド又は蛋白質の製造方法 |
EP0499990B1 (de) * | 1991-02-19 | 1996-05-15 | Takeda Chemical Industries, Ltd. | Verfahren zur Herstellung von cysteinfreien Peptiden |
WO1992017595A1 (en) * | 1991-04-01 | 1992-10-15 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Genes which influence pichia proteolytic activity, and uses therefor |
US5665539A (en) * | 1991-07-12 | 1997-09-09 | The Regents Of The University Of California | Immuno-polymerase chain reaction system for antigen detection |
US5328985A (en) * | 1991-07-12 | 1994-07-12 | The Regents Of The University Of California | Recombinant streptavidin-protein chimeras useful for conjugation of molecules in the immune system |
US5684136A (en) * | 1992-05-18 | 1997-11-04 | Genentech, Inc. | Chimeric hepatocyte growth factor (HGF) ligand variants |
EP0642585B1 (de) * | 1992-05-18 | 2005-12-28 | Genentech, Inc. | Aktivierung von Rezeptoren fähig zur Oligomerisierung durch Verwendung von fusionierten Rezeptor Liganden |
US5532142A (en) * | 1993-02-12 | 1996-07-02 | Board Of Regents, The University Of Texas System | Method of isolation and purification of fusion polypeptides |
GB9513967D0 (en) * | 1995-07-08 | 1995-09-06 | Univ Leicester | Insulin |
US6261787B1 (en) | 1996-06-03 | 2001-07-17 | Case Western Reserve University | Bifunctional molecules for delivery of therapeutics |
EP0963435B1 (de) | 1997-01-08 | 2008-05-28 | Invitrogen Corporation | Verfahren zur herstellung von proteinen |
EP1911841B1 (de) | 1997-01-08 | 2015-06-03 | Life Technologies Corporation | Verfahren zur Herstellung von Proteinen |
US6432699B1 (en) * | 1997-03-28 | 2002-08-13 | New York University | Viral vectors having chimeric envelope proteins containing the IgG-binding domain of protein A |
CA2374013A1 (en) * | 1999-05-15 | 2000-11-23 | University Of California, San Diego | Protein a based binding domains with desirable activities |
US7163686B1 (en) | 1999-05-15 | 2007-01-16 | The Regents Of The University Of California | Protein A based binding domains with desirable activities |
US7259232B1 (en) | 2000-10-27 | 2007-08-21 | Nymox Pharmaceutical Corporation | Preferred segments of neural thread protein |
US20030211970A1 (en) * | 2001-06-01 | 2003-11-13 | Samuel Nochumson | Processing of plasmid-containing fluids |
US7270960B2 (en) | 2001-08-29 | 2007-09-18 | Pacific Northwest Research Institute | Diagnosis of ovarian carcinomas |
US7375188B2 (en) * | 2005-07-29 | 2008-05-20 | Mallinckrodt Baker, Inc. | Vegetarian protein a preparation and methods thereof |
ATE485517T1 (de) | 2006-03-22 | 2010-11-15 | Viral Logic Systems Technology | Verfahren zur identifizierung von polypeptid- targets |
JP2009537145A (ja) * | 2006-05-15 | 2009-10-29 | バイラル ロジック システムズ テクノロジー コーポレーション | 免疫学的疾患および障害を治療するためのcd47と関連した組成物および方法 |
US8377448B2 (en) * | 2006-05-15 | 2013-02-19 | The Board Of Trustees Of The Leland Standford Junior University | CD47 related compositions and methods for treating immunological diseases and disorders |
JP2009544974A (ja) | 2006-07-21 | 2009-12-17 | ライフ テクノロジーズ コーポレーション | 鮮明に分解する標識タンパク質分子量標準 |
EP2099932A4 (de) * | 2006-12-06 | 2009-11-18 | Repligen Corp | Nukleinsäuren zur kodierung des rekombinanten proteins a |
US20100028904A1 (en) * | 2007-03-30 | 2010-02-04 | Centocor, Inc. | Way to obtain high expression clones of mammalian cells using a methylcellulose with fluorescent protein a or g and fluorescent screening method |
CN112662676B (zh) * | 2020-12-30 | 2023-12-29 | 南方科技大学 | 一种dna编码靶向水解蛋白的多肽或者蛋白的方法 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GR71687B (de) * | 1977-11-08 | 1983-06-21 | Genentech Inc | |
IE47890B1 (en) * | 1977-11-08 | 1984-07-11 | Genentech Inc | Polypeptide production by expression in a recombinant microbial cloning vehicle, and cloning vehicles |
US4411994A (en) * | 1978-06-08 | 1983-10-25 | The President And Fellows Of Harvard College | Protein synthesis |
IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
US4336336A (en) * | 1979-01-12 | 1982-06-22 | President And Fellows Of Harvard College | Fused gene and method of making and using same |
CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
DK582381A (da) * | 1981-01-02 | 1982-07-03 | Univ New York | Remkombinat plasmid til anvendelse ved bakteriel kloning |
SE8204811L (sv) * | 1982-08-23 | 1984-02-24 | Sven Lofdahl | Dna-fragment innefattande en signalsekvens |
SE8204810L (sv) * | 1982-08-23 | 1984-02-24 | Pharmacia Fine Chemicals Ab | Hybrid-dna-molekyl, transformerad mikroorganism och sett att framstella protein a |
SE451596B (sv) * | 1983-06-22 | 1987-10-19 | Excorim Kb | Forfarande for utvinning av protein g |
US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
US4691009A (en) * | 1984-12-26 | 1987-09-01 | Repligen Corporation | Hybrid proteins produced by an ultrahigh prokaryotic expression system |
US4900660A (en) * | 1985-11-25 | 1990-02-13 | University Of Florida | Streptococcal fc rc |
-
1983
- 1983-02-09 SE SE8300693A patent/SE8300693L/ not_active Application Discontinuation
-
1984
- 1984-02-09 DE DE8484900773T patent/DE3473298D1/de not_active Expired
- 1984-02-09 JP JP59500885A patent/JPH0634742B2/ja not_active Expired - Lifetime
- 1984-02-09 WO PCT/SE1984/000046 patent/WO1984003103A1/en active IP Right Grant
- 1984-02-09 EP EP84900773A patent/EP0135532B1/de not_active Expired
-
1988
- 1988-05-09 US US07/196,846 patent/US5100788A/en not_active Expired - Lifetime
-
1995
- 1995-04-20 JP JP7117618A patent/JPH07265078A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JPH0634742B2 (ja) | 1994-05-11 |
SE8300693D0 (sv) | 1983-02-09 |
WO1984003103A1 (en) | 1984-08-16 |
US5100788A (en) | 1992-03-31 |
EP0135532B1 (de) | 1988-08-10 |
SE8300693L (sv) | 1984-08-10 |
JPH07265078A (ja) | 1995-10-17 |
EP0135532A1 (de) | 1985-04-03 |
JPS60500480A (ja) | 1985-04-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: PHARMACIA BIOTECH AB, UPPSALA, SE |