DE3473298D1 - Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins - Google Patents

Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins

Info

Publication number
DE3473298D1
DE3473298D1 DE8484900773T DE3473298T DE3473298D1 DE 3473298 D1 DE3473298 D1 DE 3473298D1 DE 8484900773 T DE8484900773 T DE 8484900773T DE 3473298 T DE3473298 T DE 3473298T DE 3473298 D1 DE3473298 D1 DE 3473298D1
Authority
DE
Germany
Prior art keywords
immunoglobulins
polypeptides
recombinant
bind
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8484900773T
Other languages
English (en)
Inventor
Sven Lofdahl
Mathias Uhlen
Martin Lindberg
John Sjoquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia Biosystems AB
Original Assignee
Pharmacia AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia AB filed Critical Pharmacia AB
Application granted granted Critical
Publication of DE3473298D1 publication Critical patent/DE3473298D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE8484900773T 1983-02-09 1984-02-09 Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins Expired DE3473298D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8300693A SE8300693L (sv) 1983-02-09 1983-02-09 Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
PCT/SE1984/000046 WO1984003103A1 (en) 1983-02-09 1984-02-09 Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins

Publications (1)

Publication Number Publication Date
DE3473298D1 true DE3473298D1 (en) 1988-09-15

Family

ID=20349966

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484900773T Expired DE3473298D1 (en) 1983-02-09 1984-02-09 Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins

Country Status (6)

Country Link
US (1) US5100788A (de)
EP (1) EP0135532B1 (de)
JP (2) JPH0634742B2 (de)
DE (1) DE3473298D1 (de)
SE (1) SE8300693L (de)
WO (1) WO1984003103A1 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123228B1 (de) * 1983-04-25 1993-09-29 Chiron Corporation Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
WO1985000831A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of insulin-like growth factor
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
DK108685A (da) * 1984-03-19 1985-09-20 Fujisawa Pharmaceutical Co Vaekstfaktor i
US5248666A (en) * 1984-03-23 1993-09-28 Oncogen Methods for inhibiting neoplastic cell proliferation using platelet factor 4
US4745179A (en) * 1984-04-02 1988-05-17 Fujisawa Pharmaceutical Co., Ltd. 59 Valine insulin-like growth factor I and process for production thereof
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US5028530A (en) * 1985-01-28 1991-07-02 Xoma Corporation AraB promoters and method of producing polypeptides, including cecropins, by microbiological techniques
JPS61264000A (ja) * 1985-03-21 1986-11-21 イミユネツクス　コ−ポレイシヨン 標識ペプチドによるタンパク質の合成
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
EP0196056B1 (de) * 1985-03-28 1991-05-22 Chiron Corporation Expression durch Verwendung von Fusionsgenen für Proteinproduktion
US4751180A (en) * 1985-03-28 1988-06-14 Chiron Corporation Expression using fused genes providing for protein product
US5342921A (en) * 1985-03-28 1994-08-30 Chiron Corporation Superoxide dismutase fusion polypeptides for expression of mammalian proteins
US5169939A (en) * 1985-05-21 1992-12-08 Massachusetts Institute Of Technology & Pres. & Fellows Of Harvard College Chimeric antibodies
SE8505922D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering
SE8505921D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab A method to export gene products to the growth medium of gram negative bacteria
DE3545568A1 (de) * 1985-12-21 1987-07-16 Hoechst Ag Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung
SE459662B (sv) * 1986-03-21 1989-07-24 Pharmacia Ab Hybrid-dna-molekyl som kodar foer ett protein med samma igg specificitet som protein g, plasmid innehaallande densamma samt foerfarande foer framstaellning av proteinet
SE8601940D0 (sv) * 1986-04-25 1986-04-25 Kabigen Ab Preparation of fused proteins, antibodies and processes therefor
AU623589B2 (en) * 1987-03-02 1992-05-21 Bristol-Myers Squibb Company Platelet related growth regulator
US4987070A (en) * 1987-03-04 1991-01-22 Suntory Limited Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins
US5643758A (en) * 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
US5215896A (en) * 1987-03-20 1993-06-01 Creative Biomolecules, Inc. Leader sequences for the production of recombinant proteins
US5013653A (en) 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
JPH082315B1 (de) * 1987-05-29 1996-01-17 Sagami Chuo Kagaku Kenkyusho
US5212064A (en) * 1987-09-21 1993-05-18 Microgenics Corporation Solid phase non-separation enzyme complementation assay
US5243040A (en) * 1987-11-20 1993-09-07 Creative Biomolecules DNA encoding a protein which enables selective removal of immune complexes
US5084398A (en) * 1987-11-20 1992-01-28 Creative Biomolecules Selective removal of immune complexes
SE501169C2 (sv) * 1988-02-05 1994-11-28 Aake Nygren Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
SE8800981A (sv) * 1988-03-17 1988-10-14 Lars Abrahamsen A recombinant fusion protein, its use and a recombinant vector
CA2005252C (en) * 1988-12-13 2001-07-17 Nahum Sonenberg Isolation of full-length cdna clones and capped mrna
US6267964B1 (en) * 1989-08-01 2001-07-31 Affibody Technology Sweden Ab Stabilized protein or peptide conjugates able to bond albumin having extended biological half-lives
US5620923A (en) * 1989-10-12 1997-04-15 The University Of Utah Synthesis of peptides as cloned ubiquitin extensions
DE3942580A1 (de) * 1989-12-22 1991-06-27 Basf Ag Verfahren zur herstellung von hirudin
US5624822A (en) * 1989-12-22 1997-04-29 Basf Aktiengesellschaft Hirudin fusion proteins and preparation of hirudin
JPH0740924B2 (ja) * 1990-08-22 1995-05-10 エム・ディ・リサーチ株式会社 形質転換体および融合蛋白質の製造方法
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH0740926B2 (ja) * 1990-10-09 1995-05-10 エム・ディ・リサーチ株式会社 形質転換体およびペプチド前駆体タンパク質の製造方法
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
EP0499990B1 (de) * 1991-02-19 1996-05-15 Takeda Chemical Industries, Ltd. Verfahren zur Herstellung von cysteinfreien Peptiden
CA2105064A1 (en) * 1991-04-01 1992-10-02 Martin Anthony Gleeson Genes which influence pichia proteolytic activity, and uses therefor
US5665539A (en) * 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
US5328985A (en) * 1991-07-12 1994-07-12 The Regents Of The University Of California Recombinant streptavidin-protein chimeras useful for conjugation of molecules in the immune system
US5684136A (en) * 1992-05-18 1997-11-04 Genentech, Inc. Chimeric hepatocyte growth factor (HGF) ligand variants
EP0642585B1 (de) * 1992-05-18 2005-12-28 Genentech, Inc. Aktivierung von Rezeptoren fähig zur Oligomerisierung durch Verwendung von fusionierten Rezeptor Liganden
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
US6261787B1 (en) 1996-06-03 2001-07-17 Case Western Reserve University Bifunctional molecules for delivery of therapeutics
EP1911841B1 (de) 1997-01-08 2015-06-03 Life Technologies Corporation Verfahren zur Herstellung von Proteinen
ES2308800T3 (es) 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
US6432699B1 (en) * 1997-03-28 2002-08-13 New York University Viral vectors having chimeric envelope proteins containing the IgG-binding domain of protein A
EP1181052A4 (de) * 1999-05-15 2004-08-11 Univ California San Diego Bindungsdomänen, basierend auf protein a, mit gewünschten eigenschaften
US7163686B1 (en) 1999-05-15 2007-01-16 The Regents Of The University Of California Protein A based binding domains with desirable activities
US7259232B1 (en) 2000-10-27 2007-08-21 Nymox Pharmaceutical Corporation Preferred segments of neural thread protein
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
US7270960B2 (en) 2001-08-29 2007-09-18 Pacific Northwest Research Institute Diagnosis of ovarian carcinomas
US7375188B2 (en) * 2005-07-29 2008-05-20 Mallinckrodt Baker, Inc. Vegetarian protein a preparation and methods thereof
ATE485517T1 (de) 2006-03-22 2010-11-15 Viral Logic Systems Technology Verfahren zur identifizierung von polypeptid- targets
AU2007249709A1 (en) * 2006-05-15 2007-11-22 Viral Logic Systems Technology Corp. CD47 related compositions and methods for treating immunological diseases and disorders
US8377448B2 (en) * 2006-05-15 2013-02-19 The Board Of Trustees Of The Leland Standford Junior University CD47 related compositions and methods for treating immunological diseases and disorders
WO2008042495A2 (en) 2006-07-21 2008-04-10 Life Technologies Corporation Sharply resolving labeled protein molecular weight standards
US7691608B2 (en) 2006-12-06 2010-04-06 Repligen Corporation Nucleic acids encoding recombinant protein A
US20100028904A1 (en) * 2007-03-30 2010-02-04 Centocor, Inc. Way to obtain high expression clones of mammalian cells using a methylcellulose with fluorescent protein a or g and fluorescent screening method
CN112662676B (zh) * 2020-12-30 2023-12-29 南方科技大学 一种dna编码靶向水解蛋白的多肽或者蛋白的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH656640A5 (de) * 1977-11-08 1986-07-15 Genentech Inc Verfahren zur herstellung eines polypeptids.
FI783367A (fi) * 1977-11-08 1979-05-09 Genentech Inc Syntetisk dna och foerfarande foer dess framstaellning
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
FI792481A (fi) * 1978-08-11 1980-02-12 Univ California Syntes av enkaroytiskt protein genom anvaendning av mikro-organismer
US4336336A (en) * 1979-01-12 1982-06-22 President And Fellows Of Harvard College Fused gene and method of making and using same
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
GR76959B (de) * 1981-01-02 1984-09-04 Univ New York State Res Found
SE8204811L (sv) * 1982-08-23 1984-02-24 Sven Lofdahl Dna-fragment innefattande en signalsekvens
SE8204810L (sv) * 1982-08-23 1984-02-24 Pharmacia Fine Chemicals Ab Hybrid-dna-molekyl, transformerad mikroorganism och sett att framstella protein a
SE451596B (sv) * 1983-06-22 1987-10-19 Excorim Kb Forfarande for utvinning av protein g
US4801537A (en) * 1984-06-08 1989-01-31 Genex Corporation Vector for expression of polypeptides in bacilli
US4691009A (en) * 1984-12-26 1987-09-01 Repligen Corporation Hybrid proteins produced by an ultrahigh prokaryotic expression system
US4900660A (en) * 1985-11-25 1990-02-13 University Of Florida Streptococcal fc rc

Also Published As

Publication number Publication date
JPH0634742B2 (ja) 1994-05-11
SE8300693L (sv) 1984-08-10
EP0135532B1 (de) 1988-08-10
US5100788A (en) 1992-03-31
JPS60500480A (ja) 1985-04-11
JPH07265078A (ja) 1995-10-17
SE8300693D0 (sv) 1983-02-09
WO1984003103A1 (en) 1984-08-16
EP0135532A1 (de) 1985-04-03

Similar Documents

Publication Publication Date Title
DE3473298D1 (en) Method of producing and selectively isolating proteins and polypeptides, recombinant and expression vector therefor and fusion protein able to bind to the constant region of immunoglobulins
EP0324647A3 (en) Vector for the expression of fusion proteins and protein immunogens
GB2246783B (en) Fusion proteins containing n-terminal fragments of human serum albumin
GB2091268B (en) Recombinant dna-molecules and methods for protein production
PH25312A (en) Preparation of functional human urokinase proteins
DE3485723D1 (de) Verfahren und signalsequenz fuer die extrazellulare herstellung von proteinmaterial.
AU2964489A (en) Dna sequences, recombinant dna molecules and processes for producing human immune interferon-like polypeptides
EP0243929A3 (en) Polypeptide and protein derivatives and process for their preparation
ZA896715B (en) Fusion protein of paramyxovirus,method of production using recombinant baculovirus expression vector,vaccine comprising such protein and use thereof
GB8719587D0 (en) Monoclonal antibodies & peptides
EP0139676A4 (de) Herstellung und kennzeichnen von hybridoma antikörpern spezifisch gerichtet gegen gemeinsame determinanten zwischen naheliegenden aber unterschiedlichen proteinen.
GB2171703B (en) Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
EP0227110A3 (en) Human immunoglobulin g fc region protein and production thereof
EP0149241A3 (en) Dna base sequence containing regions involved in the production and secretion of a protein, recombinant dna including the whole or a part of the dna base sequence, and method of producing proteins by use of the recombinant dna
EP0170266A3 (en) Process for producing protein, and vector, recombinant dna and transformant used therefor
GB8413301D0 (en) Polypeptide and protein products
ZA892518B (en) Method for the in-vivo production and testing of proteins by recombinant gene expression in selected host-cells
HU895916D0 (en) Process for the preparation of monoclonal antibody recognaizing reg protein
GB2256870B (en) Method for enhancing recovery of recombinant proteins
IL89877A0 (en) Process for the preparation of proteins which start with n-terminal proline
NZ212690A (en) Hybridoma capable of producing monoclonal antibody against bovine lactoferrin
PT86162A (en) Recombinant protein expression
ATE36348T1 (de) Herstellungsverfahren und selektive isolierung von proteinen und polypeptiden, rekombinant und expressionsvektor dazu und schmelzprotein das zum konstanten teil von immoglobulinen binden kann.
NZ228194A (en) Monoclonal antibodies which bind to the n-terminal peptide sequence of the betasubunit of human haemoglobiin
EP0460674A3 (en) Recombinant dna and process for the production of chimaeric antibodies

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: PHARMACIA BIOTECH AB, UPPSALA, SE