DE3427679A1 - Verfahren zur untersuchung von mikroorganismenpopulation - Google Patents
Verfahren zur untersuchung von mikroorganismenpopulationInfo
- Publication number
- DE3427679A1 DE3427679A1 DE19843427679 DE3427679A DE3427679A1 DE 3427679 A1 DE3427679 A1 DE 3427679A1 DE 19843427679 DE19843427679 DE 19843427679 DE 3427679 A DE3427679 A DE 3427679A DE 3427679 A1 DE3427679 A1 DE 3427679A1
- Authority
- DE
- Germany
- Prior art keywords
- dicyanobenzene
- microorganisms
- vessel
- fluorescence
- derivative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims description 30
- 238000011835 investigation Methods 0.000 title claims description 5
- 239000000243 solution Substances 0.000 claims abstract description 17
- XQZYPMVTSDWCCE-UHFFFAOYSA-N phthalonitrile Chemical class N#CC1=CC=CC=C1C#N XQZYPMVTSDWCCE-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 6
- 108090000371 Esterases Proteins 0.000 claims abstract description 5
- 239000012085 test solution Substances 0.000 claims abstract description 4
- VXDCGBVAZDYTFK-UHFFFAOYSA-N (4-acetyloxy-2,3-dicyanophenyl) acetate Chemical compound CC(=O)OC1=CC=C(OC(C)=O)C(C#N)=C1C#N VXDCGBVAZDYTFK-UHFFFAOYSA-N 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 230000005284 excitation Effects 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- HMSLOQWSOHVMFK-UHFFFAOYSA-N (4-butanoyloxy-2,3-dicyanophenyl) butanoate Chemical compound CCCC(=O)OC1=CC=C(OC(=O)CCC)C(C#N)=C1C#N HMSLOQWSOHVMFK-UHFFFAOYSA-N 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000011534 incubation Methods 0.000 abstract description 3
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 21
- 210000004027 cell Anatomy 0.000 description 18
- 241000894006 Bacteria Species 0.000 description 17
- 239000002609 medium Substances 0.000 description 11
- 239000003242 anti bacterial agent Substances 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 8
- 210000002700 urine Anatomy 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 229940088710 antibiotic agent Drugs 0.000 description 7
- 235000013305 food Nutrition 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000011541 reaction mixture Substances 0.000 description 7
- 238000007796 conventional method Methods 0.000 description 6
- 238000001802 infusion Methods 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241000588921 Enterobacteriaceae Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- 241000588698 Erwinia Species 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 241000588769 Proteus <enterobacteria> Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000607720 Serratia Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 210000001072 colon Anatomy 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000014670 detection of bacterium Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- MPAIWVOBMLSHQA-UHFFFAOYSA-N 3,6-dihydroxybenzene-1,2-dicarbonitrile Chemical compound OC1=CC=C(O)C(C#N)=C1C#N MPAIWVOBMLSHQA-UHFFFAOYSA-N 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000590020 Achromobacter Species 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- XIURVHNZVLADCM-IUODEOHRSA-N cefalotin Chemical compound N([C@H]1[C@@H]2N(C1=O)C(=C(CS2)COC(=O)C)C(O)=O)C(=O)CC1=CC=CS1 XIURVHNZVLADCM-IUODEOHRSA-N 0.000 description 1
- 229960000603 cefalotin Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000012568 clinical material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000006787 czapek-dox agar Substances 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 230000033629 detection of yeast Effects 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002489 hematologic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000002847 impedance measurement Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 239000012907 medicinal substance Substances 0.000 description 1
- 229960000564 nitrofurantoin Drugs 0.000 description 1
- NXFQHRVNIOXGAQ-YCRREMRBSA-N nitrofurantoin Chemical compound O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)NC(=O)C1 NXFQHRVNIOXGAQ-YCRREMRBSA-N 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000001558 permutation test Methods 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 210000001635 urinary tract Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13656583A JPS6027398A (ja) | 1983-07-26 | 1983-07-26 | 微生物検査方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3427679A1 true DE3427679A1 (de) | 1985-02-14 |
| DE3427679C2 DE3427679C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-10-27 |
Family
ID=15178215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19843427679 Granted DE3427679A1 (de) | 1983-07-26 | 1984-07-26 | Verfahren zur untersuchung von mikroorganismenpopulation |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS6027398A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
| DE (1) | DE3427679A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012206239A1 (de) * | 2012-04-17 | 2013-10-17 | Hamilton Bonaduz Ag | Dosiervorrichtung, insbesondere Pipettierautomat mit Entsorgungsbehälter |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2628531B1 (fr) * | 1988-03-08 | 1992-10-02 | Chemunex Sa | Procede de recherche, de detection specifique et de numeration de micro-organismes, installation pour la mise en oeuvre dudit procede |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2729500A1 (de) * | 1976-07-01 | 1978-01-19 | Corning Glass Works | Ansatz und verfahren fuer den mikrobennachweis durch fluoreszenz |
| DE2947890A1 (de) * | 1978-11-29 | 1980-06-12 | Bioresearch Inc | Verfahren und vorrichtung zur schnellbestimmung von bakterien, insbesondere koliformen bakterien in einer fluessigkeit |
| DE3104078A1 (de) * | 1981-02-06 | 1982-08-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Verfahren zur bestimmung des ph-wertes im innern einer zelle |
| DE3117241A1 (de) * | 1981-04-30 | 1982-11-18 | Ajinomoto Co., Inc., Tokyo | Schnellverfahren zum nachweis oder zur bestimmung von mikroorganismen |
-
1983
- 1983-07-26 JP JP13656583A patent/JPS6027398A/ja active Pending
-
1984
- 1984-07-26 DE DE19843427679 patent/DE3427679A1/de active Granted
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2729500A1 (de) * | 1976-07-01 | 1978-01-19 | Corning Glass Works | Ansatz und verfahren fuer den mikrobennachweis durch fluoreszenz |
| DE2947890A1 (de) * | 1978-11-29 | 1980-06-12 | Bioresearch Inc | Verfahren und vorrichtung zur schnellbestimmung von bakterien, insbesondere koliformen bakterien in einer fluessigkeit |
| DE3104078A1 (de) * | 1981-02-06 | 1982-08-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Verfahren zur bestimmung des ph-wertes im innern einer zelle |
| DE3117241A1 (de) * | 1981-04-30 | 1982-11-18 | Ajinomoto Co., Inc., Tokyo | Schnellverfahren zum nachweis oder zur bestimmung von mikroorganismen |
Non-Patent Citations (1)
| Title |
|---|
| JP-Abstract 57-144995 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012206239A1 (de) * | 2012-04-17 | 2013-10-17 | Hamilton Bonaduz Ag | Dosiervorrichtung, insbesondere Pipettierautomat mit Entsorgungsbehälter |
| US9689884B2 (en) | 2012-04-17 | 2017-06-27 | Hamilton Bonaduz Ag | Pipetting machine having a disposal container |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3427679C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-10-27 |
| JPS6027398A (ja) | 1985-02-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |