DE2951248A1 - Verfahren zur herstellung eines indikatorsystems zur bestimmung von antigen-antikoerper-reaktionen - Google Patents
Verfahren zur herstellung eines indikatorsystems zur bestimmung von antigen-antikoerper-reaktionenInfo
- Publication number
- DE2951248A1 DE2951248A1 DE19792951248 DE2951248A DE2951248A1 DE 2951248 A1 DE2951248 A1 DE 2951248A1 DE 19792951248 DE19792951248 DE 19792951248 DE 2951248 A DE2951248 A DE 2951248A DE 2951248 A1 DE2951248 A1 DE 2951248A1
- Authority
- DE
- Germany
- Prior art keywords
- membrane
- complement
- indicator system
- plate
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 238000006243 chemical reaction Methods 0.000 title claims description 4
- 239000012528 membrane Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 13
- 206010018910 Haemolysis Diseases 0.000 claims description 10
- 230000008588 hemolysis Effects 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 5
- 229920002284 Cellulose triacetate Polymers 0.000 claims description 3
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 description 22
- 239000000427 antigen Substances 0.000 description 11
- 102000036639 antigens Human genes 0.000 description 11
- 108091007433 antigens Proteins 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 210000003743 erythrocyte Anatomy 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 5
- 229920000936 Agarose Polymers 0.000 description 4
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 4
- 206010022000 influenza Diseases 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000872 buffer Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- AZUYLZMQTIKGSC-UHFFFAOYSA-N 1-[6-[4-(5-chloro-6-methyl-1H-indazol-4-yl)-5-methyl-3-(1-methylindazol-5-yl)pyrazol-1-yl]-2-azaspiro[3.3]heptan-2-yl]prop-2-en-1-one Chemical compound ClC=1C(=C2C=NNC2=CC=1C)C=1C(=NN(C=1C)C1CC2(CN(C2)C(C=C)=O)C1)C=1C=C2C=NN(C2=CC=1)C AZUYLZMQTIKGSC-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 229910021556 Chromium(III) chloride Inorganic materials 0.000 description 2
- 208000005647 Mumps Diseases 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 229960000458 allantoin Drugs 0.000 description 2
- 229960000359 chromic chloride Drugs 0.000 description 2
- QSWDMMVNRMROPK-UHFFFAOYSA-K chromium(3+) trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Cr+3] QSWDMMVNRMROPK-UHFFFAOYSA-K 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000009969 flowable effect Effects 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 208000010805 mumps infectious disease Diseases 0.000 description 2
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 241001139382 Allantion Species 0.000 description 1
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI790598A FI58566C (fi) | 1979-02-22 | 1979-02-22 | Foerbaettrat foerfarande att framstaella indikatorsystem foer bestaemning av antigen-antikroppsreaktioner med hjaelp av hemolys i gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2951248A1 true DE2951248A1 (de) | 1980-10-30 |
Family
ID=8512415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19792951248 Withdrawn DE2951248A1 (de) | 1979-02-22 | 1979-12-19 | Verfahren zur herstellung eines indikatorsystems zur bestimmung von antigen-antikoerper-reaktionen |
Country Status (5)
| Country | Link |
|---|---|
| DE (1) | DE2951248A1 (enExample) |
| FI (1) | FI58566C (enExample) |
| FR (1) | FR2449894A1 (enExample) |
| GB (1) | GB2045432B (enExample) |
| SE (1) | SE8000236L (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4522923A (en) * | 1983-10-03 | 1985-06-11 | Genetic Diagnostics Corporation | Self-contained assay method and kit |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1508132A (en) * | 1974-05-20 | 1978-04-19 | Technicon Instr | Analysis of biological fluids |
| WO1979000044A1 (en) * | 1977-07-14 | 1979-02-08 | H Elwing | Method for the determination of biological substances by diffusion in a porous matrix or by electrophoresis |
-
1979
- 1979-02-22 FI FI790598A patent/FI58566C/fi not_active IP Right Cessation
- 1979-12-19 DE DE19792951248 patent/DE2951248A1/de not_active Withdrawn
-
1980
- 1980-01-11 SE SE8000236A patent/SE8000236L/ not_active Application Discontinuation
- 1980-01-14 FR FR8000695A patent/FR2449894A1/fr active Granted
- 1980-02-21 GB GB8005893A patent/GB2045432B/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2449894A1 (fr) | 1980-09-19 |
| GB2045432A (en) | 1980-10-29 |
| SE8000236L (sv) | 1980-08-23 |
| FI58566C (fi) | 1981-02-10 |
| GB2045432B (en) | 1983-08-17 |
| FI58566B (fi) | 1980-10-31 |
| FR2449894B1 (enExample) | 1983-10-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |