DE2037507A1 - - Google Patents
Info
- Publication number
- DE2037507A1 DE2037507A1 DE19702037507 DE2037507A DE2037507A1 DE 2037507 A1 DE2037507 A1 DE 2037507A1 DE 19702037507 DE19702037507 DE 19702037507 DE 2037507 A DE2037507 A DE 2037507A DE 2037507 A1 DE2037507 A1 DE 2037507A1
- Authority
- DE
- Germany
- Prior art keywords
- complement
- sensitized
- agar
- fixing
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000427 antigen Substances 0.000 claims description 32
- 108091007433 antigens Proteins 0.000 claims description 32
- 102000036639 antigens Human genes 0.000 claims description 32
- 230000000295 complement effect Effects 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 25
- 229920001817 Agar Polymers 0.000 claims description 17
- 210000002966 serum Anatomy 0.000 claims description 17
- 239000000499 gel Substances 0.000 claims description 16
- 238000009792 diffusion process Methods 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 6
- 239000013060 biological fluid Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 241001494479 Pecora Species 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 10
- 230000009089 cytolysis Effects 0.000 description 9
- 210000000601 blood cell Anatomy 0.000 description 7
- 108010006464 Hemolysin Proteins Proteins 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 235000010419 agar Nutrition 0.000 description 6
- 210000003743 erythrocyte Anatomy 0.000 description 6
- 239000003228 hemolysin Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 239000003349 gelling agent Substances 0.000 description 5
- 230000000951 immunodiffusion Effects 0.000 description 5
- 238000011835 investigation Methods 0.000 description 4
- 241000700199 Cavia porcellus Species 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 230000002949 hemolytic effect Effects 0.000 description 3
- 235000018977 lysine Nutrition 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000606651 Rickettsiales Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- KKEYFWRCBNTPAC-UHFFFAOYSA-N Terephthalic acid Chemical compound OC(=O)C1=CC=C(C(O)=O)C=C1 KKEYFWRCBNTPAC-UHFFFAOYSA-N 0.000 description 2
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 150000002734 metacrylic acid derivatives Chemical class 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000000405 serological effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- 229920002972 Acrylic fiber Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 229920008347 Cellulose acetate propionate Polymers 0.000 description 1
- DQEFEBPAPFSJLV-UHFFFAOYSA-N Cellulose propionate Chemical compound CCC(=O)OCC1OC(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C1OC1C(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C(COC(=O)CC)O1 DQEFEBPAPFSJLV-UHFFFAOYSA-N 0.000 description 1
- 229920002284 Cellulose triacetate Polymers 0.000 description 1
- 241000223205 Coccidioides immitis Species 0.000 description 1
- 206010014979 Epidemic typhus Diseases 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000711981 Sais Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 208000031726 Spotted Fever Group Rickettsiosis Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920006217 cellulose acetate butyrate Polymers 0.000 description 1
- 229920006218 cellulose propionate Polymers 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 201000003486 coccidioidomycosis Diseases 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940039227 diagnostic agent Drugs 0.000 description 1
- 239000000032 diagnostic agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 208000028104 epidemic louse-borne typhus Diseases 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012817 gel-diffusion technique Methods 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 150000002669 lysines Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000009589 serological test Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 201000004284 spotted fever Diseases 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US84833869A | 1969-08-07 | 1969-08-07 | |
| US00279611A US3843777A (en) | 1969-08-07 | 1972-08-10 | Hemolytic assay system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2037507A1 true DE2037507A1 (enExample) | 1971-02-18 |
Family
ID=26959785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19702037507 Pending DE2037507A1 (enExample) | 1969-08-07 | 1970-07-29 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US3843777A (enExample) |
| BE (1) | BE754428A (enExample) |
| DE (1) | DE2037507A1 (enExample) |
| FR (1) | FR2056651A5 (enExample) |
| GB (1) | GB1288003A (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4239746A (en) * | 1973-06-30 | 1980-12-16 | Dezso Istvan Bartos | Complement fixation test employing reactants in a disposable package |
| DE2333434C3 (de) * | 1973-06-30 | 1978-04-06 | Istvan D. Dr. 5024 Pulheim Bartos | Verfahren zur Durchführung serologischer Untersuchungen nach dem Prinzip der Komplementbindungsreaktion und gebrauchsfertige Schnelltestpackung hierfür |
| US4176174A (en) * | 1974-07-19 | 1979-11-27 | Burroughs Wellcome Co. | Viral antibody diagnostic test system |
| JPS5238789A (en) * | 1975-08-14 | 1977-03-25 | Sinai School Medicine | Method of identifying hematocyte type and adaptability |
| US4130395A (en) * | 1975-08-19 | 1978-12-19 | Beth Israel Medical Center | Process and apparatus for detection of specific biological factors by means of osmotic hemolysis |
| US4282002A (en) * | 1979-09-06 | 1981-08-04 | Akzona Incorporated | Sensitized sheep stroma immunoassay for rheumatoid factor |
| US4387166A (en) * | 1981-09-15 | 1983-06-07 | Anda Biologicals | Immunoassay wherein immune complex forms and ages for at least one hour |
| US4659655A (en) * | 1981-11-25 | 1987-04-21 | Bio-Response, Inc. | Method for isolating product-producing cells |
| EP0103139B1 (en) * | 1982-08-11 | 1990-01-03 | Kabushiki Kaisha Toshiba | Reagent composition for immunoassay and immunoassay using the same |
| US4874710A (en) * | 1986-02-20 | 1989-10-17 | Becton Dickinson And Company | Assay and product in which binder and liposomes are supported on a solid support |
| US6346509B1 (en) | 1997-12-05 | 2002-02-12 | Nippon Shokubai Co., Ltd. | Higher secondary alcohol alkoxylate compound composition, method for production thereof, and detergent and emulsifier using the composition |
| ITTO20070106A1 (it) * | 2007-02-14 | 2007-05-16 | Carlo Ciaiolo | Gel semisolido reattivo e relativa area di reazione, per reazioni biologiche e chimiche |
-
0
- BE BE754428D patent/BE754428A/xx unknown
-
1970
- 1970-07-23 GB GB3565070A patent/GB1288003A/en not_active Expired
- 1970-07-29 DE DE19702037507 patent/DE2037507A1/de active Pending
- 1970-08-06 FR FR7029001A patent/FR2056651A5/fr not_active Expired
-
1972
- 1972-08-10 US US00279611A patent/US3843777A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US3843777A (en) | 1974-10-22 |
| FR2056651A5 (enExample) | 1971-05-14 |
| GB1288003A (enExample) | 1972-09-06 |
| BE754428A (fr) | 1971-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE2851150C2 (de) | Verfahren zur immunologischen Untersuchung einer Serumprobe | |
| CH625625A5 (enExample) | ||
| DE2930562A1 (de) | Immuntest zur bestimmung von klassenspezifischen antikoerpern | |
| CH627281A5 (enExample) | ||
| DE2737491A1 (de) | Verfahren zur bestimmung eines immunologisch aktiven materials und system zu seiner durchfuehrung | |
| DE2037507A1 (enExample) | ||
| EP0292810A2 (de) | Einschritt-Immuntest zur Bestimmung von antigen-spezifischen Antikörpern aus einer der Immunglobulin-Klassen A, M, D oder E und dazu geeignetes Mittel | |
| EP0001223A2 (de) | Mit einer Polyhydroxyverbindung beschichteten Latex, Verfahren zur Herstellung dieses Latex, immunologisches Reagenz enthaltend diesen Latex, Verfahren zur Herstellung dieses Reagenzes, Verwendung dieses Reagenzes, Bestimmungsverfahren unter Verwendung dieses Reagenzes und Reagenziengarnitur enthaltend dieses Reagenz. | |
| DE2134928A1 (de) | Biologisches Reagens | |
| DE2604844C3 (de) | Verfahren zum Nachweis von Hepatitis B-Oberflächenantigen in menschlichem Blut | |
| DE2322562A1 (de) | Verfahren zur bestimmung eines oder mehrerer antigene in einer probe | |
| DE2419884B2 (de) | Verfahren und Vorrichtung zur Bestimmung von Trijodthyronin | |
| DE3412340C2 (de) | Substrat zur Fluoreszenz-Immunanalyse von biologischen Flüssigkeiten | |
| DE2624814C2 (de) | Verfahren und Mittel zum Nachweis oder zur Bestimmung von Antikörpern in Körperflüssigkeiten | |
| DE69013730T2 (de) | Für ganze Zellen geeigneter Satz und Verfahren zu enzymatischen Bestimmungen. | |
| DE3105555A1 (de) | Reagens zum nachweis des rheumatoidfaktors, verfahren zu seiner herstellung und seine verwendung | |
| DE3889479T2 (de) | Mit Nieder-pI-Protein beschichtete Membranstruktur und Verfahren zu ihrer Herstellung und Verwendung. | |
| DE2714751C2 (enExample) | ||
| DE2533701A1 (de) | Immunologische diagnoseprodukte | |
| DE2907228C2 (de) | Immunochemische Bestimmung zur Bestimmung der LDH↓1↓-Aktivität | |
| DE3883808T2 (de) | Testsatz, Extraktionsvorrichtung und Verfahren zur Bestimmung von Streptococcus-A-Antigen. | |
| DE69319064T2 (de) | Biologisch aktives Reagens, hergestellt aus Aldehydgruppen enthaltendem Polymer, Testausrüstung, analytisches Element und Anwendungsmethoden | |
| DE2643829A1 (de) | Verfahren zum feststellen der anwesenheit eines der hepatitis zuzuordnenden antigens | |
| DE2440930A1 (de) | Visuelles verfahren zur feststellung von syphilis und anderen treponemalen erkrankungen | |
| DE69317931T2 (de) | Sensibilisierte Erythrozyten, ein Verfahren zu deren Herstellung, und deren Verwendung |