DE2644432A1 - Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung - Google Patents

Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung

Info

Publication number
DE2644432A1
DE2644432A1 DE19762644432 DE2644432A DE2644432A1 DE 2644432 A1 DE2644432 A1 DE 2644432A1 DE 19762644432 DE19762644432 DE 19762644432 DE 2644432 A DE2644432 A DE 2644432A DE 2644432 A1 DE2644432 A1 DE 2644432A1
Authority
DE
Germany
Prior art keywords
microorganism
dap
cells
strain
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19762644432
Other languages
German (de)
English (en)
Other versions
DE2644432C2 (enExample
Inventor
Iii Roy Curtiss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp
Original Assignee
Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Corp filed Critical Research Corp
Publication of DE2644432A1 publication Critical patent/DE2644432A1/de
Application granted granted Critical
Publication of DE2644432C2 publication Critical patent/DE2644432C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
DE19762644432 1976-09-27 1976-10-01 Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung Granted DE2644432A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/727,365 US4190495A (en) 1976-09-27 1976-09-27 Modified microorganisms and method of preparing and using same

Publications (2)

Publication Number Publication Date
DE2644432A1 true DE2644432A1 (de) 1978-03-30
DE2644432C2 DE2644432C2 (enExample) 1988-12-29

Family

ID=24922359

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19762644432 Granted DE2644432A1 (de) 1976-09-27 1976-10-01 Genetisch modifizierte mikroorganismen und verfahren zu ihrer herstellung

Country Status (4)

Country Link
US (1) US4190495A (enExample)
JP (1) JPS5341477A (enExample)
DE (1) DE2644432A1 (enExample)
GB (1) GB1516458A (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422685A1 (fr) * 1978-04-14 1979-11-09 Pasteur Institut Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli
EP0012494A1 (en) * 1978-08-11 1980-06-25 The Regents Of The University Of California A DNA transfer vector, a microorganism modified by said vector and the synthesis of a eucaryotic protein by the modified microorganism
EP0219342A2 (en) 1985-10-15 1987-04-22 Genentech, Inc. Method and reagents for in vitro oligonucleotide synthesis

Families Citing this family (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221619A (en) * 1977-11-08 1993-06-22 Genentech, Inc. Method and means for microbial polypeptide expression
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US5583013A (en) * 1977-11-08 1996-12-10 Genentech, Inc. Method and means for microbial polypeptide expression
US4322497A (en) * 1978-06-02 1982-03-30 Eli Lilly And Company Process for transducing Escherichia coli K12 χ1776
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4411994A (en) 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
US6268122B1 (en) 1978-12-22 2001-07-31 Biogen, Inc. Recombinant DNA molecules and their method of production
JPS55156591A (en) * 1979-05-23 1980-12-05 Ajinomoto Co Inc Method for stabilizing property of bacteria containing plasmid
US4337314A (en) * 1979-05-23 1982-06-29 Research Corporation Genetically attenuated bacterial vaccines with multiple mutations of the same phenotype
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
DE2931999A1 (de) * 1979-08-03 1981-02-26 Schering Ag Herstellung und anwendung von neukombinierten plasmiden mit genen fuer alkalische phosphatasen
US4302544A (en) * 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4886754A (en) * 1980-01-07 1989-12-12 The University Of Rochester Recombinant bateriophage for heterologous cloning of bacillus microorganisms and method for its production
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
US6835557B1 (en) 1980-01-08 2004-12-28 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human interferon-like polypeptides
KR860001471B1 (ko) 1980-04-03 1986-09-26 비오겐 엔. 브이 인체의 섬유아세포 인터페론(IFN-β)폴리펩티드의 제조방법
US4558010A (en) * 1980-04-03 1985-12-10 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator and method of making plasminogen activator protein therefrom
US4370417A (en) * 1980-04-03 1983-01-25 Abbott Laboratories Recombinant deoxyribonucleic acid which codes for plasminogen activator
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
US5643771A (en) * 1980-05-19 1997-07-01 The Board Of Trustees Of The Leland Stanford Junior University Non-reverting live bacterial vaccines
US4357298A (en) * 1980-10-10 1982-11-02 General Electric Company Nuclear fuel assembly space arrangement
US4888170A (en) * 1981-10-22 1989-12-19 Research Corporation Vaccines obtained from antigenic gene products of recombinant genes
JPS60137291A (ja) * 1983-12-26 1985-07-20 Takeda Chem Ind Ltd 発現ベクター
FR2602792B1 (fr) * 1986-08-05 1989-10-20 Transgene Sa Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
JPS63264061A (ja) * 1987-04-23 1988-10-31 吉田精工株式会社 歯科用治療椅子の制御装置
US5387744A (en) * 1987-06-04 1995-02-07 Washington University Avirulent microbes and uses therefor: Salmonella typhi
US5855880A (en) * 1987-06-04 1999-01-05 Washington University Avirulent microbes and uses therefor
US5294441A (en) * 1987-06-04 1994-03-15 Washington University Avirulent microbes and uses therefor: salmonella typhi
US5030574A (en) * 1987-06-19 1991-07-09 Vary Patricia S Plasmidless Lac strain of bacilus megaterium QM B1551
US5037757A (en) * 1987-07-20 1991-08-06 Board Of Regents For Northern Illinois University Plasmidless strain of Bacillus megaterium QM B1551
ATE121785T1 (de) * 1987-10-07 1995-05-15 Univ Washington Verfahren zur erhaltung eines erwünschten rekombinanten gens in einer genetischen zellpopulation.
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
EP0437071A1 (en) * 1990-01-08 1991-07-17 Stratagene Novel cloning host organisms
GB9206318D0 (en) * 1992-03-24 1992-05-06 Cambridge Antibody Tech Binding substances
AU9094191A (en) * 1990-11-21 1992-06-25 Washington University Recombinant avirulent salmonella antifertility vaccines
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
US6492160B1 (en) 1991-05-15 2002-12-10 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US6225447B1 (en) 1991-05-15 2001-05-01 Cambridge Antibody Technology Ltd. Methods for producing members of specific binding pairs
US5858657A (en) * 1992-05-15 1999-01-12 Medical Research Council Methods for producing members of specific binding pairs
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
AU683221B2 (en) * 1992-09-04 1997-11-06 University Of Saskatchewan Novel bacterial vaccines using vaccine strains of pathogenic bacteria
AU711618B2 (en) * 1995-06-07 1999-10-21 Washington University Recombinant bacterial system with environmentally limited viability
FR2738842B1 (fr) * 1995-09-15 1997-10-31 Rhone Poulenc Rorer Sa Molecule d'adn circulaire a origine de replication conditionnelle, leur procede de preparation et leur utilisation en therapie genique
US7279313B2 (en) * 1995-09-15 2007-10-09 Centelion Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
US5770435A (en) * 1995-11-02 1998-06-23 University Of Chicago Mutant E. coli strain with increased succinic acid production
US5869301A (en) * 1995-11-02 1999-02-09 Lockhead Martin Energy Research Corporation Method for the production of dicarboxylic acids
US5645831A (en) * 1996-03-22 1997-07-08 Biodiscovery New Zealand Ltd. Bacillus thuringiensis strain and metabolite which are active against corn rootworm
US6221364B1 (en) 1996-11-12 2001-04-24 Albert Einstein College Of Medicine Of Yeshiva University Recombinant mycobacteria auxotrophic for diaminopimelate
US5733544A (en) * 1996-11-18 1998-03-31 University Of Saskatchewan Nematicidal bacillus strain and metabolite and methods of use thereof
US6537558B2 (en) 1997-03-31 2003-03-25 Megan Health, Inc. Methods of producing and using virulence attenuated poxR mutant bacteria
US6780405B1 (en) 2000-04-28 2004-08-24 Avant Immunotherapeutics, Inc. Regulated antigen delivery system (RADS)
US6872547B1 (en) 2000-10-11 2005-03-29 Washington University Functional balanced-lethal host-vector systems
US20040137003A1 (en) * 2001-04-30 2004-07-15 Curtiss Iii Roy Regulated antigen delivery system (rads)
US7780961B2 (en) * 2001-05-03 2010-08-24 Actogenix N.V. Self-containing Lactococcus strain
US20030194798A1 (en) 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
US7396822B2 (en) 2001-05-24 2008-07-08 Vaxiion Therapeutics, Inc. Immunogenic minicells and methods of use
US6852485B1 (en) 2002-01-22 2005-02-08 California State University Fullerton Foundation Method for identifying a compound for the treatment of microorganism infections by inhibiting energy storage and utilization in pathogens
US20030224369A1 (en) * 2002-02-25 2003-12-04 Surber Mark W. Reverse screening and target identification with minicells
WO2003072014A2 (en) 2002-02-25 2003-09-04 Mpex Bioscience, Inc. Minicell compositions and methods
US20030224444A1 (en) * 2002-02-25 2003-12-04 Sabbadini Roger A. Antibodies to native conformations of membrane proteins
US20030207833A1 (en) * 2002-02-25 2003-11-06 Neil Berkley Pharmaceutical compositions with minicells
DE60319822T2 (de) * 2002-06-19 2009-06-04 Actogenix Nv Verfahren und mittel zur erhöhung der darmabsorption
AU2003278729A1 (en) * 2002-09-01 2004-03-19 Washington University Regulated bacterial lysis for gene vaccine vector delivery and antigen release
DE602004024518D1 (de) * 2003-04-26 2010-01-21 Merck & Co Inc Vorwärtsmutationstest auf grundlage der 5-fluoruracilresistenz
WO2006055024A2 (en) * 2004-04-05 2006-05-26 Vaxiion Therapeutics, Inc. Minicells as vaccines
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
EP1919954B1 (en) 2005-08-30 2016-10-19 University of Miami Immunomodulating tumor necrosis factor receptor 25 (tnfr25) agonists, antagonists and immunotoxins
US20070111294A1 (en) * 2005-09-09 2007-05-17 Genomatica, Inc. Methods and organisms for the growth-coupled production of succinate
PT2119450E (pt) 2005-11-29 2013-04-26 Actogenix Nv Indução de tolerância da mucosa a autoantigénios de células beta de ilhéus pancreáticos
WO2008091627A2 (en) * 2007-01-22 2008-07-31 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid
ES2666658T3 (es) 2007-01-25 2018-05-07 Intrexon Actobiotics Nv Tratamiento de enfermedad inmune mediante suministro a la mucosa de antígenos
CA2702676A1 (en) 2007-05-01 2008-11-27 Synthetic Genomics, Inc. Methods of genome installation in a recipient host cell
US20100317084A1 (en) * 2007-05-10 2010-12-16 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant bacteria comprising vectors for expression of nucleic acid sequences encoding antigens
WO2009025888A2 (en) 2007-05-10 2009-02-26 The Arizona Board Of Regents For And On Behalf Of Arizona State University Regulated synthesis of antigen and/or regulated attentuation to enhance vaccine immunogenics and/or safety
CA2696018A1 (en) * 2007-08-10 2009-04-09 Genomatica, Inc. Methods for the synthesis of olefins and derivatives
WO2009046451A1 (en) * 2007-10-05 2009-04-09 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant bacterium capable of eliciting a protective immune response against c. perfringens
US7803589B2 (en) * 2008-01-22 2010-09-28 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
WO2009151728A2 (en) * 2008-03-27 2009-12-17 Genomatica, Inc. Microorganisms for the production of adipic acid and other compounds
AU2009242615A1 (en) * 2008-05-01 2009-11-05 Genomatica, Inc. Microorganisms for the production of methacrylic acid
WO2009155382A1 (en) * 2008-06-17 2009-12-23 Genomatica, Inc. Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
US20100021978A1 (en) * 2008-07-23 2010-01-28 Genomatica, Inc. Methods and organisms for production of 3-hydroxypropionic acid
US9446227B2 (en) 2008-09-12 2016-09-20 Sonescence, Inc. Ultrasonic dispersion of compositions in tissue
US20100069827A1 (en) 2008-09-12 2010-03-18 Barry Neil Silberg Pre-Surgical Prophylactic Administration of Antibiotics and Therapeutic Agents
WO2010045620A1 (en) 2008-10-17 2010-04-22 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant bacterium capable of eliciting an immune response against streptococcus pneumoniae
US20100184173A1 (en) * 2008-11-14 2010-07-22 Genomatica, Inc. Microorganisms for the production of methyl ethyl ketone and 2-butanol
AU2009327490A1 (en) * 2008-12-16 2011-07-28 Genomatica, Inc. Microorganisms and methods for conversion of syngas and other carbon sources to useful products
US9163219B2 (en) * 2009-04-14 2015-10-20 Arizona Board Of Regents On Behalf Of Arizona State University Single expression vector for generation of a virus with a segmented genome
BRPI1013505A2 (pt) 2009-04-30 2018-02-14 Genomatica Inc organismos para a produção de isopropanol, n-butanol, e isobutanol
US9017983B2 (en) * 2009-04-30 2015-04-28 Genomatica, Inc. Organisms for the production of 1,3-butanediol
SI2427544T1 (sl) 2009-05-07 2019-11-29 Genomatica Inc Mikroorganizmi in metode za biosintezo adipata, heksametilendiamina in 6-aminokaprojske kisline
KR20120036851A (ko) * 2009-05-15 2012-04-18 게노마티카 인코포레이티드 사이클로헥사논의 제조를 위한 유기체
GB2483595B (en) 2009-05-22 2017-03-29 The Arizona Board Of Regents For And On Behalf Of Arizona State Univ Recombinant bacterium and methods of antigen and nucleic acid delivery
US9045742B2 (en) * 2009-05-29 2015-06-02 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant Edwardsiella bacterium
US8420375B2 (en) * 2009-06-10 2013-04-16 Genomatica, Inc. Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol
SG178125A1 (en) 2009-08-03 2012-03-29 Univ Miami Method for in vivo expansion of t regulatory cells
BR112012002643A2 (pt) 2009-08-05 2018-08-28 Genomatica Inc ácido tereftalático semissintético por meio de micro-organismos que produzem ácido mucônico
SG179048A1 (en) 2009-09-09 2012-04-27 Genomatica Inc Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
GB2473523A (en) * 2009-09-10 2011-03-16 Solus Scient Solutions Ltd Nalidixic acid and lithium in a base medium for the culture of Listeria
KR20120083908A (ko) 2009-10-13 2012-07-26 게노마티카 인코포레이티드 1,4-부탄다이올, 4-하이드록시부탄알, 4-하이드록시부티릴-ｃｏａ, 푸트레신 및 관련 화합물의 제조를 위한 미생물 및 관련 방법
BR112012009332A2 (pt) 2009-10-23 2015-09-15 Genomatica Inc micro-organismo para a produção de anilina
BR112012014062A2 (pt) * 2009-12-10 2017-04-04 Genomatica Inc organismo microbiano que não ocorre naturalmente e método para produzir 1,3 bdo
US9062297B2 (en) 2010-01-13 2015-06-23 The Arizona Board Of Regents For And On Behalf Of Arizona State University Yersinia pestis vaccine
EP2526185A4 (en) 2010-01-22 2013-07-24 Univ Arizona BACTERIUM WITH REGULATED RFAH NUCLEIC ACID
PH12012501526A1 (en) * 2010-01-29 2018-03-21 Genomatica Inc Microorganisms and methods for the biosynthesis of p-toluate and terephthalate
US8637286B2 (en) * 2010-02-23 2014-01-28 Genomatica, Inc. Methods for increasing product yields
US8048661B2 (en) * 2010-02-23 2011-11-01 Genomatica, Inc. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes
US9023636B2 (en) 2010-04-30 2015-05-05 Genomatica, Inc. Microorganisms and methods for the biosynthesis of propylene
WO2011140171A2 (en) 2010-05-05 2011-11-10 Genomatica, Inc. Microorganisms and methods for the biosynthesis of butadiene
US9598697B2 (en) 2010-05-28 2017-03-21 The Arizona Board Of Regents For And On Behalf Of Arizona State University Recombinant bacterium to decrease tumor growth
US9255283B2 (en) 2010-07-02 2016-02-09 The Arizona Board Of Regents For And On Behalf Of Arizona State University Compositions and methods for bacterial lysis and neutral lipid production
MX2013001071A (es) 2010-07-26 2013-06-28 Genomatica Inc Microorganismos y metodos para la biosintesis de aromaticos, 2, 4-pentadienoato y 1, 3-butadieno.
EP2675474B1 (en) 2011-02-15 2019-01-16 Vaxiion Therapeutics, LLC Therapeutic compositions and methods for antibody and fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
US9303264B2 (en) 2012-05-18 2016-04-05 The Arizona Board Of Regents For And On Behalf Of Arizona State University Photosynthetic microorganisms expressing thermostable lipase
KR20210041631A (ko) 2013-01-09 2021-04-15 유니버시티 오브 마이애미 TL1A-Ig 융합 단백질을 사용한 T 조절 세포의 조절을 위한 조성물 및 방법
US9580718B2 (en) 2013-06-17 2017-02-28 Arizona Board Of Regents On Behalf Of Arizona State University Attenuated live bacteria with increased acid resistance and methods of use thereof
US9956388B2 (en) 2014-06-04 2018-05-01 Sonescence, Inc. Systems and methods for therapeutic agent delivery
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ATCC-Katalog, 11. Aufl., 1974, S. 54 *
ATCC-Katalog, 12. Aufl., 1976, S.55-62 u. 268-270 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422685A1 (fr) * 1978-04-14 1979-11-09 Pasteur Institut Vecteurs, derives du bacteriophage lambda, permettant l'insertion et l'expression d'un gene, procaryote ou eucaryote, sous le controle du promoteur et de l'operateur de l'operon lactose de la bacterie escherichia coli
EP0012494A1 (en) * 1978-08-11 1980-06-25 The Regents Of The University Of California A DNA transfer vector, a microorganism modified by said vector and the synthesis of a eucaryotic protein by the modified microorganism
EP0219342A2 (en) 1985-10-15 1987-04-22 Genentech, Inc. Method and reagents for in vitro oligonucleotide synthesis

Also Published As

Publication number Publication date
DE2644432C2 (enExample) 1988-12-29
GB1516458A (en) 1978-07-05
JPS5341477A (en) 1978-04-14
JPS6246152B2 (enExample) 1987-09-30
US4190495A (en) 1980-02-26

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