DE2447025A1 - Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierung - Google Patents
Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierungInfo
- Publication number
- DE2447025A1 DE2447025A1 DE19742447025 DE2447025A DE2447025A1 DE 2447025 A1 DE2447025 A1 DE 2447025A1 DE 19742447025 DE19742447025 DE 19742447025 DE 2447025 A DE2447025 A DE 2447025A DE 2447025 A1 DE2447025 A1 DE 2447025A1
- Authority
- DE
- Germany
- Prior art keywords
- tissue culture
- reagents
- detection
- cells
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims description 10
- 238000001514 detection method Methods 0.000 title claims description 9
- 230000003460 anti-nuclear Effects 0.000 title claims description 8
- 230000000087 stabilizing effect Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 claims description 26
- 210000001519 tissue Anatomy 0.000 claims description 17
- 239000002274 desiccant Substances 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 210000003855 cell nucleus Anatomy 0.000 claims description 4
- 230000000890 antigenic effect Effects 0.000 claims description 3
- 206010025135 lupus erythematosus Diseases 0.000 claims description 3
- 238000003759 clinical diagnosis Methods 0.000 claims 1
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 12
- 239000000427 antigen Substances 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 10
- 108091007433 antigens Proteins 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 102000011931 Nucleoproteins Human genes 0.000 description 3
- 108010061100 Nucleoproteins Proteins 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 210000003292 kidney cell Anatomy 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- DLYUQMMRRRQYAE-UHFFFAOYSA-N tetraphosphorus decaoxide Chemical compound O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 208000027932 Collagen disease Diseases 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010036030 Polyarthritis Diseases 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 206010039710 Scleroderma Diseases 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 229910052925 anhydrite Inorganic materials 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 201000001981 dermatomyositis Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 201000006292 polyarteritis nodosa Diseases 0.000 description 1
- 208000030428 polyarticular arthritis Diseases 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Rehabilitation Therapy (AREA)
- Biotechnology (AREA)
- Rheumatology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19742447025 DE2447025A1 (de) | 1974-10-02 | 1974-10-02 | Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierung |
| NL7511361A NL7511361A (nl) | 1974-10-02 | 1975-09-26 | Reagens voor het aantonen van antinucleaire fac- toren en werkwijze voor het stabiliseren daarvan. |
| GB39746/75A GB1520646A (en) | 1974-10-02 | 1975-09-29 | Reagent for the detection of antinuclear factors and process for stabilizing it |
| LU73486A LU73486A1 (enrdf_load_stackoverflow) | 1974-10-02 | 1975-09-30 | |
| IT27789/75A IT1042984B (it) | 1974-10-02 | 1975-09-30 | Reagente per il riconoscimento di pattori antinucleari e un processo per la sua stabilizzazione |
| SE7510969A SE7510969L (sv) | 1974-10-02 | 1975-09-30 | Reagens for pavisande av antinukleera faktorer och ett forfarande for dess stabilisering |
| FI752742A FI752742A7 (enrdf_load_stackoverflow) | 1974-10-02 | 1975-09-30 | |
| CH1274675A CH620520A5 (en) | 1974-10-02 | 1975-10-01 | Reagents for detecting antinuclear factors |
| IE2146/75A IE41739B1 (en) | 1974-10-02 | 1975-10-01 | Reagent for the detection of antinuclear factors and process for stabilizing it |
| DK442675A DK442675A (da) | 1974-10-02 | 1975-10-01 | Reagens til pavisning af antinukleere faktorer og en fremgangsmade til dets stabilisering |
| AT749975A AT355226B (de) | 1974-10-02 | 1975-10-01 | Verfahren zur konservierung der antigenen eigenschaften von zellkernbestandteilen in gewebekulturzellen |
| JP50118286A JPS5161629A (enrdf_load_stackoverflow) | 1974-10-02 | 1975-10-02 | |
| BE160640A BE834134A (fr) | 1974-10-02 | 1975-10-02 | Reactif pour la detection de facteurs antinucleaires et son procede de stabilisation |
| FR7530205A FR2287043A1 (fr) | 1974-10-02 | 1975-10-02 | Reactif pour la detection de facteurs antinucleaires et son procede de stabilisation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19742447025 DE2447025A1 (de) | 1974-10-02 | 1974-10-02 | Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2447025A1 true DE2447025A1 (de) | 1976-04-08 |
Family
ID=5927338
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19742447025 Withdrawn DE2447025A1 (de) | 1974-10-02 | 1974-10-02 | Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierung |
Country Status (14)
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0206779A1 (en) * | 1985-06-21 | 1986-12-30 | Modern Diagnostics, Inc. | Detecting antinuclear antibody |
| JP2532651B2 (ja) * | 1988-03-30 | 1996-09-11 | ヘキストジャパン株式会社 | 抗核抗体測定用器具の製法 |
-
1974
- 1974-10-02 DE DE19742447025 patent/DE2447025A1/de not_active Withdrawn
-
1975
- 1975-09-26 NL NL7511361A patent/NL7511361A/xx unknown
- 1975-09-29 GB GB39746/75A patent/GB1520646A/en not_active Expired
- 1975-09-30 IT IT27789/75A patent/IT1042984B/it active
- 1975-09-30 LU LU73486A patent/LU73486A1/xx unknown
- 1975-09-30 FI FI752742A patent/FI752742A7/fi not_active Application Discontinuation
- 1975-09-30 SE SE7510969A patent/SE7510969L/xx unknown
- 1975-10-01 CH CH1274675A patent/CH620520A5/de not_active IP Right Cessation
- 1975-10-01 IE IE2146/75A patent/IE41739B1/en unknown
- 1975-10-01 AT AT749975A patent/AT355226B/de not_active IP Right Cessation
- 1975-10-01 DK DK442675A patent/DK442675A/da unknown
- 1975-10-02 JP JP50118286A patent/JPS5161629A/ja active Pending
- 1975-10-02 BE BE160640A patent/BE834134A/xx unknown
- 1975-10-02 FR FR7530205A patent/FR2287043A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| ATA749975A (de) | 1979-07-15 |
| FI752742A7 (enrdf_load_stackoverflow) | 1976-04-03 |
| AT355226B (de) | 1980-02-25 |
| NL7511361A (nl) | 1976-04-06 |
| FR2287043B1 (enrdf_load_stackoverflow) | 1980-01-11 |
| IE41739L (en) | 1976-04-02 |
| IE41739B1 (en) | 1980-03-12 |
| DK442675A (da) | 1976-04-03 |
| FR2287043A1 (fr) | 1976-04-30 |
| SE7510969L (sv) | 1976-04-05 |
| GB1520646A (en) | 1978-08-09 |
| JPS5161629A (enrdf_load_stackoverflow) | 1976-05-28 |
| CH620520A5 (en) | 1980-11-28 |
| BE834134A (fr) | 1976-04-02 |
| LU73486A1 (enrdf_load_stackoverflow) | 1977-05-11 |
| IT1042984B (it) | 1980-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Davies | Observations on the epidemiology of Rift Valley fever in Kenya | |
| DE69213826T2 (de) | Teststreifen mit Merocyanin und Nitro- oder Nitroso-substituierten polyhalogenierten Phenolsulfonphthaleinen als Protein-Indikatoren | |
| CH646607A5 (de) | Haemostatisches mittel und verfahren zu seiner herstellung. | |
| DE1816712A1 (de) | Reaktive Teilchen fuer biologische Untersuchungen und Verfahren zu ihrer Herstellung | |
| DE2546166A1 (de) | Gegerbte thrombozyten | |
| CH626999A5 (en) | Process for the preparation of a stable erythrocyte preparation | |
| DE2455369A1 (de) | Traeger fuer immunologische bestimmungen | |
| DE2134928A1 (de) | Biologisches Reagens | |
| DE1961541A1 (de) | Immunologisches Indikatorreagens | |
| EP0008720B1 (de) | Diagnostisches Mittel und Verfahren zum Nachweis von antimitochondrialen oder antinukleären Antikörpern | |
| DE2447025A1 (de) | Reagens zum nachweis von antinukleaeren faktoren und ein verfahren zu dessen stabilisierung | |
| DE2703523C2 (de) | Verdünnungsmittel für den Rubella- Virus-Hämagglutinations-Hemmungstest | |
| DE2728077A1 (de) | Verfahren zum faerben von mikroorganismen | |
| DE2037507A1 (enrdf_load_stackoverflow) | ||
| DE2025718C3 (de) | Verfahren zum Stabilisieren von lyophillsierten Erythrozyten | |
| DE2608733A1 (de) | Reagens zur bestimmung von fibrinogen, fibrinspaltprodukten und fibrinogenspaltprodukten im blut und verfahren zur herstellung desselben | |
| DE1954728C3 (de) | Pufferlösung zur Verwendung in einem Hämagglutinationstest | |
| DE19900135A1 (de) | Verfahren zur Bestimmung der Temperatur einer einzelnen Zelle in einer Zellprobe oder Gewebebiopsie sowie Verfahren zu dessen Verwendung | |
| DE1806418A1 (de) | Eiweissartiges Reagens und Herstellungsverfahren hierfuer | |
| AT225351B (de) | Verfahren zur Gewinnung organspezifischer Wirkstoffe fermentartiger Aktivität | |
| DE2949824A1 (de) | Verfahren zur stabilisierung von lymphozyten/thrombozytennmischsuspensionen | |
| AT364091B (de) | Durchfuehrung eines haemagglutinationstests | |
| AT258466B (de) | Verfahren und Reagenz zur Bestimmung von menschlichem chorionischem Gonadotrophin | |
| Jones | Diagnosis of plant troubles with diphenylamine | |
| DE1773841C (de) | Verfahren zur Vorbereitung, Konservierung und zum Transport von Blut und Serumproben |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8130 | Withdrawal |