DE2336414A1 - Vorrichtung fuer grosstechnische gelelektrophorese - Google Patents
Vorrichtung fuer grosstechnische gelelektrophoreseInfo
- Publication number
- DE2336414A1 DE2336414A1 DE19732336414 DE2336414A DE2336414A1 DE 2336414 A1 DE2336414 A1 DE 2336414A1 DE 19732336414 DE19732336414 DE 19732336414 DE 2336414 A DE2336414 A DE 2336414A DE 2336414 A1 DE2336414 A1 DE 2336414A1
- Authority
- DE
- Germany
- Prior art keywords
- gel
- chamber
- chambers
- wash
- washout
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001962 electrophoresis Methods 0.000 title description 7
- 239000007788 liquid Substances 0.000 claims description 22
- 238000001816 cooling Methods 0.000 claims description 19
- 239000000872 buffer Substances 0.000 claims description 16
- 239000012528 membrane Substances 0.000 claims description 14
- 229920003023 plastic Polymers 0.000 claims description 12
- 239000004033 plastic Substances 0.000 claims description 12
- 239000000110 cooling liquid Substances 0.000 claims description 10
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 238000001502 gel electrophoresis Methods 0.000 claims description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 239000011534 wash buffer Substances 0.000 claims 1
- 239000000499 gel Substances 0.000 description 47
- -1 methyl methyl Chemical group 0.000 description 7
- 239000004698 Polyethylene Substances 0.000 description 6
- 229920000573 polyethylene Polymers 0.000 description 6
- 229920002401 polyacrylamide Polymers 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000007513 Hemoglobin A Human genes 0.000 description 1
- 108010085682 Hemoglobin A Proteins 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Natural products C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB3359572A GB1359944A (en) | 1972-07-18 | 1972-07-18 | Apparatus for gel electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2336414A1 true DE2336414A1 (de) | 1974-01-31 |
Family
ID=10354953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19732336414 Pending DE2336414A1 (de) | 1972-07-18 | 1973-07-17 | Vorrichtung fuer grosstechnische gelelektrophorese |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS4946498A (enExample) |
| AU (1) | AU475968B2 (enExample) |
| CA (1) | CA1009181A (enExample) |
| DE (1) | DE2336414A1 (enExample) |
| FR (1) | FR2192875B1 (enExample) |
| GB (1) | GB1359944A (enExample) |
| IL (1) | IL42596A (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2656162A1 (de) * | 1975-12-12 | 1977-09-29 | Aminkemi Ab | Verfahren zur erzeugung einer ph-funktion fuer die elektrophoretische trennung |
| DE2808344A1 (de) * | 1977-03-25 | 1978-10-12 | Instrumentation Specialties Co | Verfahren und vorrichtung zur konzentrierung von proben |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3046729C2 (de) * | 1980-12-11 | 1985-05-15 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Thermostatische Platte für eine Elektrophoreseeinrichtung |
| DE3337668C2 (de) * | 1983-10-17 | 1985-12-05 | Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck | Verfahren zur Elektroelution elektrisch geladener Makromoleküle |
| DE3337669C2 (de) * | 1983-10-17 | 1989-09-21 | Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck | Gerät zur Elektroelution elektrisch geladener Makromoleküle |
| US6129828A (en) | 1996-09-06 | 2000-10-10 | Nanogen, Inc. | Apparatus and methods for active biological sample preparation |
| US6071394A (en) | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
| EP1180238A1 (de) * | 1999-05-19 | 2002-02-20 | Bilatec AG | Vorrichtung und verfahren zur isolierung von elektrisch geladenen molekülen |
| US6887362B2 (en) | 2002-02-06 | 2005-05-03 | Nanogen, Inc. | Dielectrophoretic separation and immunoassay methods on active electronic matrix devices |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3346479A (en) * | 1964-04-09 | 1967-10-10 | Scientific Industries | Preparative separation by a combination of gel separation and electrophoresis |
| US3657260A (en) * | 1970-09-25 | 1972-04-18 | Robert C Mcleester | Electrophoresis apparatus |
-
1972
- 1972-07-18 GB GB3359572A patent/GB1359944A/en not_active Expired
-
1973
- 1973-06-25 IL IL42596A patent/IL42596A/en unknown
- 1973-06-26 AU AU57338/73A patent/AU475968B2/en not_active Expired
- 1973-07-16 FR FR7325994A patent/FR2192875B1/fr not_active Expired
- 1973-07-16 JP JP48079358A patent/JPS4946498A/ja active Pending
- 1973-07-17 DE DE19732336414 patent/DE2336414A1/de active Pending
- 1973-07-17 CA CA176,579A patent/CA1009181A/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2656162A1 (de) * | 1975-12-12 | 1977-09-29 | Aminkemi Ab | Verfahren zur erzeugung einer ph-funktion fuer die elektrophoretische trennung |
| DE2808344A1 (de) * | 1977-03-25 | 1978-10-12 | Instrumentation Specialties Co | Verfahren und vorrichtung zur konzentrierung von proben |
Also Published As
| Publication number | Publication date |
|---|---|
| IL42596A (en) | 1976-03-31 |
| CA1009181A (en) | 1977-04-26 |
| FR2192875B1 (enExample) | 1976-11-12 |
| GB1359944A (en) | 1974-07-17 |
| IL42596A0 (en) | 1973-08-29 |
| AU475968B2 (en) | 1976-09-09 |
| JPS4946498A (enExample) | 1974-05-04 |
| FR2192875A1 (enExample) | 1974-02-15 |
| AU5733873A (en) | 1975-01-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHN | Withdrawal |