DE2224131B2 - Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiert - Google Patents
Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiertInfo
- Publication number
- DE2224131B2 DE2224131B2 DE2224131A DE2224131A DE2224131B2 DE 2224131 B2 DE2224131 B2 DE 2224131B2 DE 2224131 A DE2224131 A DE 2224131A DE 2224131 A DE2224131 A DE 2224131A DE 2224131 B2 DE2224131 B2 DE 2224131B2
- Authority
- DE
- Germany
- Prior art keywords
- cholesterol
- enzyme
- deep
- buffer solution
- surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims description 38
- 238000000034 method Methods 0.000 title claims description 30
- 235000012000 cholesterol Nutrition 0.000 title claims description 19
- 108010089254 Cholesterol oxidase Proteins 0.000 title claims description 12
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 title claims description 4
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 title claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 28
- 108090000790 Enzymes Proteins 0.000 claims description 28
- 239000004094 surface-active agent Substances 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 14
- 239000007853 buffer solution Substances 0.000 claims description 10
- KTUQUZJOVNIKNZ-UHFFFAOYSA-N butan-1-ol;hydrate Chemical compound O.CCCCO KTUQUZJOVNIKNZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 150000001450 anions Chemical class 0.000 claims description 5
- 210000002421 cell wall Anatomy 0.000 claims description 4
- 230000006378 damage Effects 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000002736 nonionic surfactant Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 description 15
- 239000008363 phosphate buffer Substances 0.000 description 11
- 241000187654 Nocardia Species 0.000 description 8
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 230000029087 digestion Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241001251094 Formica Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 229930182558 Sterol Natural products 0.000 description 2
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000003432 sterols Chemical class 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- RQNOFUZGXHSHOT-UHFFFAOYSA-N 1-(diethylamino)ethanol Chemical group CCN(CC)C(C)O RQNOFUZGXHSHOT-UHFFFAOYSA-N 0.000 description 1
- DWJGXSMZIIIRGR-UHFFFAOYSA-N 2-(diethylamino)ethynol Chemical group CCN(CC)C#CO DWJGXSMZIIIRGR-UHFFFAOYSA-N 0.000 description 1
- ZCXGMSGCBDSEOY-UHFFFAOYSA-N 2-benzothiazolsulfonic acid Chemical compound C1=CC=C2SC(S(=O)(=O)O)=NC2=C1 ZCXGMSGCBDSEOY-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/826—Actinomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/872—Nocardia
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (19)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2224133A DE2224133B2 (de) | 1972-05-17 | 1972-05-17 | Verwendung von bestimmten Mikroorganismen-Stämmen zur Gewinnung von Cholesterinoxydase, welche Cholesterin mit O tief 2 zu Cholestenon + H tief 2 O tief 2 oxydiert |
| DE2224131A DE2224131B2 (de) | 1972-05-17 | 1972-05-17 | Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiert |
| DE2307518A DE2307518C3 (de) | 1972-05-17 | 1973-02-15 | Gewinnung von Cholesterinoxidase |
| AT362473A AT323101B (de) | 1972-05-17 | 1973-04-24 | Verfahren zur isolierung von cholesterinoxidase |
| DK255573A DK131784C (da) | 1972-05-17 | 1973-05-09 | Fremgangsmade til udvinding af cholesterinoxidase |
| CH681373A CH592108A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-17 | 1973-05-14 | |
| NL7306737.A NL164604C (nl) | 1972-05-17 | 1973-05-15 | Werkwijze voor het bereiden van een cholesteroloxydase- preparaat. |
| US05/360,490 US4144129A (en) | 1972-05-17 | 1973-05-15 | Cholesteroloxidase and method for its production from microorganisms |
| BE131116A BE799542A (fr) | 1972-05-17 | 1973-05-15 | Cholesterol-oxydase et son procede de preparation, |
| ZA733259A ZA733259B (en) | 1972-05-17 | 1973-05-15 | Improvements relating to enzymes |
| GB2308173A GB1391876A (en) | 1972-05-17 | 1973-05-15 | Cholesterol oxidase |
| IL42292A IL42292A (en) | 1972-05-17 | 1973-05-16 | Cholesterol oxidase and process for obtaining the same |
| IT24187/73A IT987645B (it) | 1972-05-17 | 1973-05-16 | Ottenimento di colesterinossi dasi |
| SE7306914A SE413246B (sv) | 1972-05-17 | 1973-05-16 | Forfarande for framstellning av kolesteroloxidas |
| HUBO001437 HU169971B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-17 | 1973-05-16 | |
| SU7301934663A SU584801A3 (ru) | 1972-05-17 | 1973-05-17 | Способ получени фермента, окисл ющего холестерин до перекиси водорода и холестенона |
| FR7317899A FR2184968B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-17 | 1973-05-17 | |
| JP5515673A JPS5728552B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-17 | 1973-05-17 | |
| NL8000305A NL8000305A (nl) | 1972-05-17 | 1980-01-17 | Werkwijze voor het kweken van een micro-organisme. |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2224133A DE2224133B2 (de) | 1972-05-17 | 1972-05-17 | Verwendung von bestimmten Mikroorganismen-Stämmen zur Gewinnung von Cholesterinoxydase, welche Cholesterin mit O tief 2 zu Cholestenon + H tief 2 O tief 2 oxydiert |
| DE2224131A DE2224131B2 (de) | 1972-05-17 | 1972-05-17 | Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiert |
| DE2307518A DE2307518C3 (de) | 1972-05-17 | 1973-02-15 | Gewinnung von Cholesterinoxidase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2224131A1 DE2224131A1 (de) | 1973-12-20 |
| DE2224131B2 true DE2224131B2 (de) | 1974-06-27 |
Family
ID=27184423
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2224133A Granted DE2224133B2 (de) | 1972-05-17 | 1972-05-17 | Verwendung von bestimmten Mikroorganismen-Stämmen zur Gewinnung von Cholesterinoxydase, welche Cholesterin mit O tief 2 zu Cholestenon + H tief 2 O tief 2 oxydiert |
| DE2224131A Granted DE2224131B2 (de) | 1972-05-17 | 1972-05-17 | Verfahren zur Gewinnung des Enzyms Cholesterinoxydase, welches Cholesterin mit O tief 2 zu Cholestenon und H tief 2 O tief 2 oxydiert |
| DE2307518A Expired DE2307518C3 (de) | 1972-05-17 | 1973-02-15 | Gewinnung von Cholesterinoxidase |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2224133A Granted DE2224133B2 (de) | 1972-05-17 | 1972-05-17 | Verwendung von bestimmten Mikroorganismen-Stämmen zur Gewinnung von Cholesterinoxydase, welche Cholesterin mit O tief 2 zu Cholestenon + H tief 2 O tief 2 oxydiert |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2307518A Expired DE2307518C3 (de) | 1972-05-17 | 1973-02-15 | Gewinnung von Cholesterinoxidase |
Country Status (13)
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5724875B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1974-03-07 | 1982-05-26 | ||
| DE2456586C3 (de) | 1974-11-29 | 1979-09-27 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verwendung von bestimmten Mikroorganismenstämmen zur Gewinnung von Cholesterinoxidase, welche Cholesterin mit O2 zu Cholestenon + H2 O2 oxidiert |
| CA1067844A (en) * | 1975-12-11 | 1979-12-11 | Eastman Kodak Company | Method and medium for producing cholesterol oxidase |
| US4043870A (en) * | 1976-05-11 | 1977-08-23 | The Upjohn Company | Process of purifying cholesterol oxidase |
| JPS5530670A (en) * | 1978-08-25 | 1980-03-04 | Seiko Instr & Electronics Ltd | Electronic watch |
| JPS56109586A (en) * | 1980-02-05 | 1981-08-31 | Cpc International Inc | Production of fixed glucoseisomerase |
| US5171688A (en) * | 1988-08-30 | 1992-12-15 | Cholestech Corporation | Self-corrected assay device |
| US5156954A (en) * | 1989-03-08 | 1992-10-20 | Cholestech Corporation | Assay device and method using a signal-modulating compound |
| CA2002890C (en) * | 1988-11-14 | 1999-12-14 | Susan Long | Expression of dna sequences derived from nocardioform microorganisms |
| JP2514087B2 (ja) * | 1989-01-06 | 1996-07-10 | 富士写真フイルム株式会社 | 総コレステロ―ル分析要素 |
| US5114350A (en) * | 1989-03-08 | 1992-05-19 | Cholestech Corporation | Controlled-volume assay apparatus |
| US5340539A (en) * | 1989-03-16 | 1994-08-23 | Chemtrak, Inc. | Non-instrumented cholesterol assay |
| JP2786679B2 (ja) * | 1989-07-24 | 1998-08-13 | 旭化成工業株式会社 | 新規なω位カルボキシアルコール酸化酵素 |
| US5206148A (en) * | 1989-07-24 | 1993-04-27 | Asahi Kasei Kogyo Kabushiki Kaisha | Method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof |
| US5238816A (en) * | 1989-07-24 | 1993-08-24 | Asahi Kasei Kogyo Kabushiki Kaisha | Omega carboxyalcohol oxidase enzyme |
| US5110724A (en) * | 1990-04-02 | 1992-05-05 | Cholestech Corporation | Multi-analyte assay device |
| CA2456180C (en) * | 2001-08-06 | 2011-10-11 | Astrazeneca Ab | Aqueous dispersion comprising stable nanoparticles of a water-insoluble active and an excipient like middle chain triglycerides (mct) |
| ES2252358T3 (es) * | 2002-04-09 | 2006-05-16 | Cholestech Corporation | Metodo y dispositivo de ensayo para la cuantificacion del colesterol asociado a lipoproteinas de alta densidad. |
| DE602008002825D1 (de) * | 2007-01-09 | 2010-11-11 | Cholestech Corp | Vorrichtung und verfahren zur messung des ldl-assoziierten cholesterols |
| WO2022032148A1 (en) | 2020-08-06 | 2022-02-10 | Mate Precision Technologies Inc. | Tooling base assembly |
| WO2022032178A1 (en) | 2020-08-06 | 2022-02-10 | Mate Precision Technologies Inc. | Vise assembly |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3607093A (en) * | 1968-02-15 | 1971-09-21 | Schering Corp | Devices for testing biological liquids |
| FR2047147A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1969-05-06 | 1971-03-12 | Kyowa Hakko Kogyo Kk | |
| GB1385319A (en) * | 1971-09-22 | 1975-02-26 | Nat Res Dev | Enzyme preparations |
-
1972
- 1972-05-17 DE DE2224133A patent/DE2224133B2/de active Granted
- 1972-05-17 DE DE2224131A patent/DE2224131B2/de active Granted
-
1973
- 1973-02-15 DE DE2307518A patent/DE2307518C3/de not_active Expired
- 1973-04-24 AT AT362473A patent/AT323101B/de not_active IP Right Cessation
- 1973-05-09 DK DK255573A patent/DK131784C/da active
- 1973-05-14 CH CH681373A patent/CH592108A5/xx not_active IP Right Cessation
- 1973-05-15 BE BE131116A patent/BE799542A/xx not_active IP Right Cessation
- 1973-05-15 US US05/360,490 patent/US4144129A/en not_active Expired - Lifetime
- 1973-05-15 NL NL7306737.A patent/NL164604C/xx active
- 1973-05-15 GB GB2308173A patent/GB1391876A/en not_active Expired
- 1973-05-16 IL IL42292A patent/IL42292A/en unknown
- 1973-05-16 SE SE7306914A patent/SE413246B/xx unknown
- 1973-05-16 IT IT24187/73A patent/IT987645B/it active
- 1973-05-17 JP JP5515673A patent/JPS5728552B2/ja not_active Expired
- 1973-05-17 FR FR7317899A patent/FR2184968B1/fr not_active Expired
-
1980
- 1980-01-17 NL NL8000305A patent/NL8000305A/nl not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DE2307518C3 (de) | 1979-07-19 |
| DE2307518B2 (de) | 1978-10-19 |
| GB1391876A (en) | 1975-04-23 |
| DK131784B (da) | 1975-09-01 |
| NL7306737A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1973-11-20 |
| IL42292A0 (en) | 1973-07-30 |
| FR2184968A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1973-12-28 |
| IL42292A (en) | 1976-12-31 |
| US4144129A (en) | 1979-03-13 |
| AT323101B (de) | 1975-06-25 |
| CH592108A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-10-14 |
| JPS4947582A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1974-05-08 |
| FR2184968B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1977-12-30 |
| DE2224133B2 (de) | 1974-06-20 |
| NL164604C (nl) | 1981-01-15 |
| DE2224131A1 (de) | 1973-12-20 |
| DE2224133A1 (de) | 1973-12-20 |
| SE413246B (sv) | 1980-05-12 |
| IT987645B (it) | 1975-03-20 |
| NL8000305A (nl) | 1980-06-30 |
| DK131784C (da) | 1976-02-09 |
| DE2307518A1 (de) | 1974-10-10 |
| JPS5728552B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1982-06-17 |
| BE799542A (fr) | 1973-11-16 |
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| Date | Code | Title | Description |
|---|---|---|---|
| C3 | Grant after two publication steps (3rd publication) | ||
| E77 | Valid patent as to the heymanns-index 1977 |