DE2153405A1 - Vorrichtung zum Bestimmen des prozentualen Anteils von Partikelarten in einem Medium - Google Patents
Vorrichtung zum Bestimmen des prozentualen Anteils von Partikelarten in einem MediumInfo
- Publication number
- DE2153405A1 DE2153405A1 DE19712153405 DE2153405A DE2153405A1 DE 2153405 A1 DE2153405 A1 DE 2153405A1 DE 19712153405 DE19712153405 DE 19712153405 DE 2153405 A DE2153405 A DE 2153405A DE 2153405 A1 DE2153405 A1 DE 2153405A1
- Authority
- DE
- Germany
- Prior art keywords
- leukocytes
- particles
- count
- properties
- counts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims description 54
- 210000000265 leukocyte Anatomy 0.000 claims description 165
- 210000004369 blood Anatomy 0.000 claims description 77
- 239000008280 blood Substances 0.000 claims description 77
- 210000004027 cell Anatomy 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 36
- 238000010521 absorption reaction Methods 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 24
- 230000008569 process Effects 0.000 claims description 15
- 238000001514 detection method Methods 0.000 claims description 10
- 230000003595 spectral effect Effects 0.000 claims description 9
- 230000000875 corresponding effect Effects 0.000 claims description 5
- 238000000149 argon plasma sintering Methods 0.000 claims description 4
- 230000002596 correlated effect Effects 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims 1
- 210000003746 feather Anatomy 0.000 claims 1
- 239000000523 sample Substances 0.000 description 67
- 238000004458 analytical method Methods 0.000 description 39
- 210000003979 eosinophil Anatomy 0.000 description 23
- 210000003651 basophil Anatomy 0.000 description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 210000004698 lymphocyte Anatomy 0.000 description 15
- 210000001616 monocyte Anatomy 0.000 description 15
- 210000000440 neutrophil Anatomy 0.000 description 14
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 12
- 230000004069 differentiation Effects 0.000 description 11
- 238000010186 staining Methods 0.000 description 10
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 8
- 238000011161 development Methods 0.000 description 8
- 210000003743 erythrocyte Anatomy 0.000 description 8
- 239000000834 fixative Substances 0.000 description 7
- 239000003086 colorant Substances 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000004040 coloring Methods 0.000 description 5
- 230000000877 morphologic effect Effects 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- 230000002934 lysing effect Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 238000003909 pattern recognition Methods 0.000 description 3
- 238000000790 scattering method Methods 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 101100043261 Caenorhabditis elegans spop-1 gene Proteins 0.000 description 2
- 241000270295 Serpentes Species 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000007872 degassing Methods 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 1
- 101100189378 Caenorhabditis elegans pat-3 gene Proteins 0.000 description 1
- 235000007575 Calluna vulgaris Nutrition 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 101150062523 bath-39 gene Proteins 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 1
- 238000012567 pattern recognition method Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8535370A | 1970-10-30 | 1970-10-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2153405A1 true DE2153405A1 (de) | 1972-05-18 |
Family
ID=22191047
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712153405 Pending DE2153405A1 (de) | 1970-10-30 | 1971-10-27 | Vorrichtung zum Bestimmen des prozentualen Anteils von Partikelarten in einem Medium |
Country Status (11)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3736027A1 (de) * | 1987-10-24 | 1989-05-03 | Gerhard Dipl Phys Artmann | Verfahren zur ermittlung der zu einem bestimmten zeitpunkt vorliegenden form von zellen und einrichtung zur durchfuehrung des verfahrens |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3873204A (en) * | 1970-01-14 | 1975-03-25 | Bio Physics Systems Inc | Optical extinction photoanalysis apparatus for small particles |
| US3851156A (en) * | 1972-09-05 | 1974-11-26 | Green James E | Analysis method and apparatus utilizing color algebra and image processing techniques |
| US3923397A (en) * | 1974-05-29 | 1975-12-02 | Dolive Stephen E | Hematocrit measuring method |
| US3930726A (en) * | 1974-05-29 | 1976-01-06 | Reginald C. Shuck | System for measuring volumetric ratios of liquid suspended solids |
| US4028056A (en) * | 1976-04-20 | 1977-06-07 | Technicon Instruments Corporation | Substance separation technique |
| US4071891A (en) * | 1976-04-30 | 1978-01-31 | Barrows George H | Electronic calculator - register for hematology differentials |
| DE2628790C2 (de) * | 1976-06-26 | 1978-07-06 | Draegerwerk Ag, 2400 Luebeck | Gasmeß- und Warnvorrichtung mit einem von dem nachzuweisenden Gas durchströmten Prüfröhrchen |
| US4072421A (en) * | 1976-08-30 | 1978-02-07 | Technicon Instruments Corporation | Method and apparatus for optical discrimination of particles |
| US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
| US4447883A (en) * | 1981-05-26 | 1984-05-08 | Technicon Instruments Corporation | Coincidence-error correcting apparatus and method |
| CA1194315A (en) * | 1982-08-16 | 1985-10-01 | Technicon Instruments Corporation | Volumetric pumping apparatus and method for supplying fluids to sheath stream flow cells |
| US4492752A (en) * | 1982-09-03 | 1985-01-08 | Ortho Diagnostics Systems Inc. | Method for discriminating between unstained and absorbing dye stained cells |
| US4542518A (en) * | 1982-11-03 | 1985-09-17 | Anthony Thomas E | Electronic blood cell counter |
| US4989978A (en) * | 1986-04-04 | 1991-02-05 | Technicon Instruments Corporation | Method and apparatus for determining the count per unit volume of particles |
| US5011286A (en) * | 1989-08-03 | 1991-04-30 | Met One, Inc. | Multisensor particle counter utilizing a single energy source |
| EP0598663B1 (en) * | 1992-11-19 | 2000-02-02 | Sysmex Corporation | A pretreatment method for blood analysis |
| JP2763468B2 (ja) * | 1992-12-25 | 1998-06-11 | 株式会社日立製作所 | 光散乱を用いた液体内の粒子分類装置 |
| GB9405028D0 (en) * | 1994-03-15 | 1994-04-27 | Counting Tech Ltd | Fluid diluter |
| US5599501A (en) * | 1994-11-10 | 1997-02-04 | Ciba Corning Diagnostics Corp. | Incubation chamber |
| US7092078B2 (en) * | 2003-03-31 | 2006-08-15 | Nihon Kohden Corporation | Flow cytometer for classifying leukocytes and method for determining detection angle range of the same |
| CN103250661A (zh) * | 2013-04-26 | 2013-08-21 | 中国水产科学研究院黄海水产研究所 | 一种海月水母活体染色方法 |
| CA2993218A1 (en) * | 2015-07-21 | 2017-01-26 | Fluidsens International Inc. | System and method for detection of particles in liquid or in air |
| EP3521826B1 (en) | 2016-09-28 | 2024-03-13 | Terumo Kabushiki Kaisha | Method, composition, and chip for detecting analysis object in blood sample |
| US11983912B2 (en) * | 2018-02-15 | 2024-05-14 | Verily Life Sciences Llc | Pathology predictions on unstained tissue |
| US10632909B1 (en) | 2018-10-11 | 2020-04-28 | Ford Global Technlogies, Llc | Alert method and assembly using sounds emitted from an electrified vehicle powertrain |
| US10636411B1 (en) | 2018-10-11 | 2020-04-28 | Ford Global Technologies, Llc | Electrified vehicle powertrain sound control method and assembly |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2847162A (en) * | 1951-11-02 | 1958-08-12 | Meyer Ernest William | Counting of particles |
| US2875666A (en) * | 1953-07-13 | 1959-03-03 | Ohio Commw Eng Co | Method of simultaneously counting red and white blood cells |
| US2824488A (en) * | 1954-08-25 | 1958-02-25 | Nat Res Corp | Apparatus for grading fibers |
| US3413464A (en) * | 1965-04-29 | 1968-11-26 | Ibm | Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution |
| US3512002A (en) * | 1965-06-14 | 1970-05-12 | Robert W Decker | Radiation sensitive counting method and apparatus |
| NL137001C (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1965-10-20 | 1900-01-01 | ||
| US3523733A (en) * | 1966-01-05 | 1970-08-11 | Technicon Corp | Method and apparatus for particle counting |
| US3510632A (en) * | 1966-02-14 | 1970-05-05 | Strandberg Eng Lab Inc | Digital stretch and speed indicating apparatus |
| US3503684A (en) * | 1966-11-09 | 1970-03-31 | Perkin Elmer Corp | Method and apparatus for detecting mitotic blood cells on a blood cell sample slide |
| US3497690A (en) * | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
-
1970
- 1970-10-30 US US00085353A patent/US3740143A/en not_active Expired - Lifetime
-
1971
- 1971-09-08 CA CA122,339A patent/CA939927A/en not_active Expired
- 1971-09-10 AU AU33333/71A patent/AU455680B2/en not_active Expired
- 1971-09-24 NL NLAANVRAGE7113140,A patent/NL168336C/xx not_active IP Right Cessation
- 1971-09-30 BE BE773365A patent/BE773365A/xx not_active IP Right Cessation
- 1971-10-21 GB GB4892871A patent/GB1346836A/en not_active Expired
- 1971-10-27 DE DE19712153405 patent/DE2153405A1/de active Pending
- 1971-10-28 SE SE7113721A patent/SE388692B/xx unknown
- 1971-10-29 FR FR7138909A patent/FR2111956B1/fr not_active Expired
- 1971-10-29 CH CH1582871A patent/CH547493A/xx not_active IP Right Cessation
- 1971-10-29 JP JP46086182A patent/JPS5843687B1/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3736027A1 (de) * | 1987-10-24 | 1989-05-03 | Gerhard Dipl Phys Artmann | Verfahren zur ermittlung der zu einem bestimmten zeitpunkt vorliegenden form von zellen und einrichtung zur durchfuehrung des verfahrens |
Also Published As
| Publication number | Publication date |
|---|---|
| NL168336B (nl) | 1981-10-16 |
| US3740143A (en) | 1973-06-19 |
| FR2111956A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-06-09 |
| CA939927A (en) | 1974-01-15 |
| AU455680B2 (en) | 1974-11-21 |
| BE773365A (fr) | 1972-03-30 |
| AU3333371A (en) | 1973-03-15 |
| JPS5843687B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1983-09-28 |
| FR2111956B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1976-03-26 |
| CH547493A (de) | 1974-03-29 |
| NL168336C (nl) | 1982-03-16 |
| NL7113140A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1972-05-03 |
| SE388692B (sv) | 1976-10-11 |
| GB1346836A (en) | 1974-02-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHW | Rejection |