GB1346836A - Automatic apparatus for determining the percentage population of particulates in a medium - Google Patents
Automatic apparatus for determining the percentage population of particulates in a mediumInfo
- Publication number
- GB1346836A GB1346836A GB4892871A GB4892871A GB1346836A GB 1346836 A GB1346836 A GB 1346836A GB 4892871 A GB4892871 A GB 4892871A GB 4892871 A GB4892871 A GB 4892871A GB 1346836 A GB1346836 A GB 1346836A
- Authority
- GB
- United Kingdom
- Prior art keywords
- path
- sample
- buffered
- mixed
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 abstract 12
- 210000004027 cell Anatomy 0.000 abstract 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 4
- 210000004369 blood Anatomy 0.000 abstract 4
- 239000008280 blood Substances 0.000 abstract 4
- 239000000975 dye Substances 0.000 abstract 4
- 210000000265 leukocyte Anatomy 0.000 abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 210000003979 eosinophil Anatomy 0.000 abstract 2
- 210000003743 erythrocyte Anatomy 0.000 abstract 2
- 239000012530 fluid Substances 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 230000002934 lysing effect Effects 0.000 abstract 2
- 239000012528 membrane Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000000980 acid dye Substances 0.000 abstract 1
- 210000003651 basophil Anatomy 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 210000001616 monocyte Anatomy 0.000 abstract 1
- XIVJNRXPRQKFRZ-UHFFFAOYSA-N naphthalen-1-yl butanoate Chemical compound C1=CC=C2C(OC(=O)CCC)=CC=CC2=C1 XIVJNRXPRQKFRZ-UHFFFAOYSA-N 0.000 abstract 1
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 abstract 1
- 210000000440 neutrophil Anatomy 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 210000002381 plasma Anatomy 0.000 abstract 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract 1
- 229930182490 saponin Natural products 0.000 abstract 1
- 150000007949 saponins Chemical class 0.000 abstract 1
- 239000001488 sodium phosphate Substances 0.000 abstract 1
- 229910000162 sodium phosphate Inorganic materials 0.000 abstract 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
-
- G01N2015/016—
Abstract
1346836 Determination of leukocytes TECHNICON INSTRUMENTS CORP 21 Oct 1971 [30 Oct 1970] 48928/71 Heading B1X [Also in Divisions Gl and G4] In an apparatus for determining the population of each type of leukocyt (white cells) in a blood sample, blood samples aspirated into a tubing system 3 flow as a wash liquid segmented steam into three paths 15, 17, 19 and then into four paths AI, AII, AIII, AIV. Each path has fed into it reagents to (a) dye a particular group of leukocytes; (b) disolve away the erythrocytes and (c) to stain the remaining membranes so that their refractive index is equal to that of the blood plasma. Each path thus delivers to a nozzle 157 a stream of fluid in which only the selected leucocytes are visible. The streams of fluid from nozzles 157 flow into the centre of four counting cells 65, 83, 101, 129 respectively while a pressurized sheathing liquid from inlet 169 fills the rest of the cell. Suitable synchronising means is provided (not shown) to ensure that individual parts of one blood sample arrive at the counting cells simultaneously and they are drawn through at the same speed by a constant pressure source by pipes 177. The counting cells each include a light source 183 and photocells for detecting direct and scattered light and feeding circuits which calculate the numbers of each type of leukocyte (see Divisions G1 and G4). In the common part of paths AI and AII the blood sample at 15 is first fixed by a 10% formalin solution buffered with sodium phosphate to pH 7.0, segmented by air at 33 and is then mixed with acetic acid from 41 which acts as a lysing agent to remove the red blood cells. The samples pass through heating and cooling bath as indicated in the figure. The sample is then divided between the two paths AI and AII. In path AI the sample is first mixed with a substrate of hydrogen peroxide, at 49, and a dark dye, at 51, comprising a solution of 4-chloro-1-napthol in 2.2'-dioxyethanol, buffered to pH 4.5 again segmented by air at 55. This stains both eosinophils and neutrophils. Propylene glycol is then added at 60 to match the red cell membrances. In path All the sample is first mixed with hydrogen peroxide at 67 and an acid dye, at 69, similar to the dark dye above but buffered to pH 3.5 and at room temperature. This stains only the eosinophils. Propylene glycol is then added at 77 as before. In path AIII the sample is first mixed with a lysing agent and dye, at 85, comprising .1% neutral red and 1% Saponin buffered to pH 5.5 which stains only the basophils. It is then fixed by a formalin solution at 91 and match again with propylene glycol at 95. In path AIV the sample is first fixed by a solution of 20% formalin buffered with phosphate to pH 6, at 103. Then the stream is mixed with a substrate of naphthol butyrate at 111 and a coupling reagent hexazonium pararosanilin, at 115 which stains only the monocytes. Acetic acid at 119 and propylene glycol at 123 are added to lyse the red cells and match the membranes as before. The large lyphocytes which are not stained are separately counted in path AI by detecting different sizes of pulses from the photocells.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8535370A | 1970-10-30 | 1970-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1346836A true GB1346836A (en) | 1974-02-13 |
Family
ID=22191047
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB4892871A Expired GB1346836A (en) | 1970-10-30 | 1971-10-21 | Automatic apparatus for determining the percentage population of particulates in a medium |
Country Status (11)
Country | Link |
---|---|
US (1) | US3740143A (en) |
JP (1) | JPS5843687B1 (en) |
AU (1) | AU455680B2 (en) |
BE (1) | BE773365A (en) |
CA (1) | CA939927A (en) |
CH (1) | CH547493A (en) |
DE (1) | DE2153405A1 (en) |
FR (1) | FR2111956B1 (en) |
GB (1) | GB1346836A (en) |
NL (1) | NL168336C (en) |
SE (1) | SE388692B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103250661A (en) * | 2013-04-26 | 2013-08-21 | 中国水产科学研究院黄海水产研究所 | Aurelia vital staining method |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3873204A (en) * | 1970-01-14 | 1975-03-25 | Bio Physics Systems Inc | Optical extinction photoanalysis apparatus for small particles |
US3851156A (en) * | 1972-09-05 | 1974-11-26 | Green James E | Analysis method and apparatus utilizing color algebra and image processing techniques |
US3930726A (en) * | 1974-05-29 | 1976-01-06 | Reginald C. Shuck | System for measuring volumetric ratios of liquid suspended solids |
US3923397A (en) * | 1974-05-29 | 1975-12-02 | Dolive Stephen E | Hematocrit measuring method |
US4028056A (en) * | 1976-04-20 | 1977-06-07 | Technicon Instruments Corporation | Substance separation technique |
US4071891A (en) * | 1976-04-30 | 1978-01-31 | Barrows George H | Electronic calculator - register for hematology differentials |
DE2628790C2 (en) * | 1976-06-26 | 1978-07-06 | Draegerwerk Ag, 2400 Luebeck | Gas measuring and warning device with a test tube through which the gas to be detected flows |
US4072421A (en) * | 1976-08-30 | 1978-02-07 | Technicon Instruments Corporation | Method and apparatus for optical discrimination of particles |
US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
US4447883A (en) * | 1981-05-26 | 1984-05-08 | Technicon Instruments Corporation | Coincidence-error correcting apparatus and method |
CA1194315A (en) * | 1982-08-16 | 1985-10-01 | Technicon Instruments Corporation | Volumetric pumping apparatus and method for supplying fluids to sheath stream flow cells |
US4492752A (en) * | 1982-09-03 | 1985-01-08 | Ortho Diagnostics Systems Inc. | Method for discriminating between unstained and absorbing dye stained cells |
US4542518A (en) * | 1982-11-03 | 1985-09-17 | Anthony Thomas E | Electronic blood cell counter |
US4989978A (en) * | 1986-04-04 | 1991-02-05 | Technicon Instruments Corporation | Method and apparatus for determining the count per unit volume of particles |
DE3736027A1 (en) * | 1987-10-24 | 1989-05-03 | Gerhard Dipl Phys Artmann | Method for determining the shape of cells which prevails at a specific instant and device for carrying out the method |
US5011286A (en) * | 1989-08-03 | 1991-04-30 | Met One, Inc. | Multisensor particle counter utilizing a single energy source |
DE69327775T2 (en) * | 1992-11-19 | 2000-06-21 | Sysmex Corp | Pretreatment procedure for blood analysis |
JP2763468B2 (en) * | 1992-12-25 | 1998-06-11 | 株式会社日立製作所 | Classification device for particles in liquids using light scattering |
GB9405028D0 (en) * | 1994-03-15 | 1994-04-27 | Counting Tech Ltd | Fluid diluter |
US5599501A (en) * | 1994-11-10 | 1997-02-04 | Ciba Corning Diagnostics Corp. | Incubation chamber |
US7092078B2 (en) * | 2003-03-31 | 2006-08-15 | Nihon Kohden Corporation | Flow cytometer for classifying leukocytes and method for determining detection angle range of the same |
EP3325945B1 (en) * | 2015-07-21 | 2024-03-06 | Fluidsens International Inc. | System and method for detection of foreign particles in a liquid |
CN109477837B (en) | 2016-09-28 | 2022-09-16 | 泰尔茂株式会社 | Method, composition and chip for detecting analyte in blood sample |
US10632909B1 (en) | 2018-10-11 | 2020-04-28 | Ford Global Technlogies, Llc | Alert method and assembly using sounds emitted from an electrified vehicle powertrain |
US10636411B1 (en) | 2018-10-11 | 2020-04-28 | Ford Global Technologies, Llc | Electrified vehicle powertrain sound control method and assembly |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2847162A (en) * | 1951-11-02 | 1958-08-12 | Meyer Ernest William | Counting of particles |
US2875666A (en) * | 1953-07-13 | 1959-03-03 | Ohio Commw Eng Co | Method of simultaneously counting red and white blood cells |
US2824488A (en) * | 1954-08-25 | 1958-02-25 | Nat Res Corp | Apparatus for grading fibers |
US3413464A (en) * | 1965-04-29 | 1968-11-26 | Ibm | Method for measuring the nucleic acid in biological cells after enhancement in an acidic solution |
US3512002A (en) * | 1965-06-14 | 1970-05-12 | Robert W Decker | Radiation sensitive counting method and apparatus |
NL137001C (en) * | 1965-10-20 | 1900-01-01 | ||
US3523733A (en) * | 1966-01-05 | 1970-08-11 | Technicon Corp | Method and apparatus for particle counting |
US3510632A (en) * | 1966-02-14 | 1970-05-05 | Strandberg Eng Lab Inc | Digital stretch and speed indicating apparatus |
US3503684A (en) * | 1966-11-09 | 1970-03-31 | Perkin Elmer Corp | Method and apparatus for detecting mitotic blood cells on a blood cell sample slide |
US3497690A (en) * | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
-
1970
- 1970-10-30 US US00085353A patent/US3740143A/en not_active Expired - Lifetime
-
1971
- 1971-09-08 CA CA122,339A patent/CA939927A/en not_active Expired
- 1971-09-10 AU AU33333/71A patent/AU455680B2/en not_active Expired
- 1971-09-24 NL NLAANVRAGE7113140,A patent/NL168336C/en not_active IP Right Cessation
- 1971-09-30 BE BE773365A patent/BE773365A/en not_active IP Right Cessation
- 1971-10-21 GB GB4892871A patent/GB1346836A/en not_active Expired
- 1971-10-27 DE DE19712153405 patent/DE2153405A1/en active Pending
- 1971-10-28 SE SE7113721A patent/SE388692B/en unknown
- 1971-10-29 FR FR7138909A patent/FR2111956B1/fr not_active Expired
- 1971-10-29 CH CH1582871A patent/CH547493A/en not_active IP Right Cessation
- 1971-10-29 JP JP46086182A patent/JPS5843687B1/ja active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103250661A (en) * | 2013-04-26 | 2013-08-21 | 中国水产科学研究院黄海水产研究所 | Aurelia vital staining method |
Also Published As
Publication number | Publication date |
---|---|
DE2153405A1 (en) | 1972-05-18 |
NL7113140A (en) | 1972-05-03 |
FR2111956A1 (en) | 1972-06-09 |
BE773365A (en) | 1972-03-30 |
CH547493A (en) | 1974-03-29 |
AU455680B2 (en) | 1974-11-21 |
SE388692B (en) | 1976-10-11 |
AU3333371A (en) | 1973-03-15 |
NL168336B (en) | 1981-10-16 |
CA939927A (en) | 1974-01-15 |
FR2111956B1 (en) | 1976-03-26 |
NL168336C (en) | 1982-03-16 |
US3740143A (en) | 1973-06-19 |
JPS5843687B1 (en) | 1983-09-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |