DE19955558C2 - Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren - Google Patents

Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren

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Publication number
DE19955558C2
DE19955558C2 DE19955558A DE19955558A DE19955558C2 DE 19955558 C2 DE19955558 C2 DE 19955558C2 DE 19955558 A DE19955558 A DE 19955558A DE 19955558 A DE19955558 A DE 19955558A DE 19955558 C2 DE19955558 C2 DE 19955558C2
Authority
DE
Germany
Prior art keywords
vectors
adenovirus
adenoviral
cells
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE19955558A
Other languages
German (de)
English (en)
Other versions
DE19955558A1 (de
Inventor
Stefan Kochanek
Gudrun Schiedner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cevec Pharmaceuticals GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE19955558A priority Critical patent/DE19955558C2/de
Application filed by Individual filed Critical Individual
Priority to DE29924144U priority patent/DE29924144U1/de
Priority to ES00979539T priority patent/ES2211647T3/es
Priority to AT00979539T priority patent/ATE257512T1/de
Priority to CA002391591A priority patent/CA2391591C/en
Priority to DE50004995T priority patent/DE50004995D1/de
Priority to CZ20021709A priority patent/CZ300124B6/cs
Priority to SI200030289T priority patent/SI1230354T1/xx
Priority to HU0203387A priority patent/HU227440B1/hu
Priority to JP2001538494A priority patent/JP4456790B2/ja
Priority to IL14929100A priority patent/IL149291A0/xx
Priority to PT00979539T priority patent/PT1230354E/pt
Priority to DK00979539T priority patent/DK1230354T3/da
Priority to PL357495A priority patent/PL205966B1/pl
Priority to PCT/EP2000/010992 priority patent/WO2001036615A2/de
Priority to CNB008159017A priority patent/CN100412201C/zh
Priority to EP00979539A priority patent/EP1230354B1/de
Priority to AU16990/01A priority patent/AU784003B2/en
Priority to TR2004/00402T priority patent/TR200400402T4/xx
Publication of DE19955558A1 publication Critical patent/DE19955558A1/de
Priority to IL149291A priority patent/IL149291A/en
Application granted granted Critical
Publication of DE19955558C2 publication Critical patent/DE19955558C2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/10011Adenoviridae
    • C12N2710/10311Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351Methods of production or purification of viral material
    • C12N2710/10352Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/40Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/40Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
    • C12N2810/405Vectors comprising RGD peptide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/50Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/50Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6009Vectors comprising as targeting moiety peptide derived from defined protein from viruses dsDNA viruses
    • C12N2810/6018Adenoviridae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00Vectors comprising a targeting moiety
    • C12N2810/50Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C12N2810/85Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
    • C12N2810/859Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from immunoglobulins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Manufacturing Of Steel Electrode Plates (AREA)
DE19955558A 1999-11-18 1999-11-18 Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren Expired - Fee Related DE19955558C2 (de)

Priority Applications (20)

Application Number Priority Date Filing Date Title
DE29924144U DE29924144U1 (de) 1999-11-18 1999-11-18 Permanente Amniozyten-Zelllinie
DE19955558A DE19955558C2 (de) 1999-11-18 1999-11-18 Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren
PL357495A PL205966B1 (pl) 1999-11-18 2000-11-07 Ciągła amniocytarna linia komórkowa, sposób jej wytwarzania, zastosowanie amniocytów, zastosowanie cząsteczki kwasu nukleinowego, zastosowanie ciągłej amniocytarnej linii komórkowej i sposób wytwarzania wektorów przenoszących geny i/lub mutantów adenowirusowych
CA002391591A CA2391591C (en) 1999-11-18 2000-11-07 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors
DE50004995T DE50004995D1 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren
CZ20021709A CZ300124B6 (cs) 1999-11-18 2000-11-07 Permanentní bunecná linie amniocytu, zpusob její prípravy a její použití k produkci vektoru pro prenos genu
SI200030289T SI1230354T1 (en) 1999-11-18 2000-11-07 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors
HU0203387A HU227440B1 (en) 1999-11-18 2000-11-07 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors
JP2001538494A JP4456790B2 (ja) 1999-11-18 2000-11-07 永久羊膜細胞株、その生成、および遺伝子移入ベクターの生成のための使用
IL14929100A IL149291A0 (en) 1999-11-18 2000-11-07 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors
ES00979539T ES2211647T3 (es) 1999-11-18 2000-11-07 Linea celular permanente de amniocitos, su preparacion y su uso para la preparacion de vectores de transferencia de genes.
DK00979539T DK1230354T3 (da) 1999-11-18 2000-11-07 Permanent amniocytcellelinje, frembringelse deraf og anvendelse deraf til frembringelse af genoverføringsvektorer
AT00979539T ATE257512T1 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren
PCT/EP2000/010992 WO2001036615A2 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren
CNB008159017A CN100412201C (zh) 1999-11-18 2000-11-07 永久性羊水细胞系,其制备和用于制备基因转移载体的用途
EP00979539A EP1230354B1 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren
AU16990/01A AU784003B2 (en) 1999-11-18 2000-11-07 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors
TR2004/00402T TR200400402T4 (tr) 1999-11-18 2000-11-07 Kalıcı aminosit hücre türü, bunun üretim ve gen transfer vektörlerinin üretimindeki kullanımı.
PT00979539T PT1230354E (pt) 1999-11-18 2000-11-07 Linha celular permanente de amniocitos sua producao e sua utilizacao para a producao de vectores de transferencia de genes
IL149291A IL149291A (en) 1999-11-18 2002-04-23 Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19955558A DE19955558C2 (de) 1999-11-18 1999-11-18 Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren

Publications (2)

Publication Number Publication Date
DE19955558A1 DE19955558A1 (de) 2001-06-07
DE19955558C2 true DE19955558C2 (de) 2003-03-20

Family

ID=7929523

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19955558A Expired - Fee Related DE19955558C2 (de) 1999-11-18 1999-11-18 Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren
DE50004995T Expired - Lifetime DE50004995D1 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE50004995T Expired - Lifetime DE50004995D1 (de) 1999-11-18 2000-11-07 Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren

Country Status (16)

Country Link
EP (1) EP1230354B1 (enExample)
JP (1) JP4456790B2 (enExample)
CN (1) CN100412201C (enExample)
AT (1) ATE257512T1 (enExample)
AU (1) AU784003B2 (enExample)
CA (1) CA2391591C (enExample)
CZ (1) CZ300124B6 (enExample)
DE (2) DE19955558C2 (enExample)
DK (1) DK1230354T3 (enExample)
ES (1) ES2211647T3 (enExample)
HU (1) HU227440B1 (enExample)
IL (2) IL149291A0 (enExample)
PL (1) PL205966B1 (enExample)
PT (1) PT1230354E (enExample)
TR (1) TR200400402T4 (enExample)
WO (1) WO2001036615A2 (enExample)

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KR101805938B1 (ko) 2009-10-15 2018-01-10 얀센 백신스 앤드 프리벤션 비.브이. 고밀도 세포 배양액에서 아데노바이러스의 정제 방법
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KR101427200B1 (ko) * 2011-11-24 2014-08-07 주식회사 바이로메드 아데노바이러스 생산 신규 세포주 및 그의 용도
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EP2662451A1 (en) * 2012-05-07 2013-11-13 Stefan Kochanek Nucleic acid construct and use of the same
WO2014060594A1 (en) * 2012-10-19 2014-04-24 Cevec Pharmaceuticals Gmbh Production of a hcmv based vaccine in human amniocyte cell lines
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