DE1908833C3 - Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli - Google Patents

Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli

Info

Publication number
DE1908833C3
DE1908833C3 DE1908833A DE1908833A DE1908833C3 DE 1908833 C3 DE1908833 C3 DE 1908833C3 DE 1908833 A DE1908833 A DE 1908833A DE 1908833 A DE1908833 A DE 1908833A DE 1908833 C3 DE1908833 C3 DE 1908833C3
Authority
DE
Germany
Prior art keywords
asparaginase
gel
cells
chromatography
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE1908833A
Other languages
German (de)
English (en)
Other versions
DE1908833A1 (de
DE1908833B2 (de
Inventor
Hans Ulrich Dr.Rer.Nat. 8132 Tutzing Bergmeyer
Karlfried Gawehn
Wolfgang Dr.Phil. Nat. 8132 Garatshausen Gruber
Hans Moellering
Waldemar Thum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to DE1908833A priority Critical patent/DE1908833C3/de
Priority to GB1231982D priority patent/GB1231982A/en
Priority to US10031A priority patent/US3684659A/en
Publication of DE1908833A1 publication Critical patent/DE1908833A1/de
Publication of DE1908833B2 publication Critical patent/DE1908833B2/de
Application granted granted Critical
Publication of DE1908833C3 publication Critical patent/DE1908833C3/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE1908833A 1969-02-21 1969-02-21 Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli Expired DE1908833C3 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE1908833A DE1908833C3 (de) 1969-02-21 1969-02-21 Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli
GB1231982D GB1231982A (enExample) 1969-02-21 1970-01-14
US10031A US3684659A (en) 1969-02-21 1970-02-09 Process for the enrichment and purification of l-asparaginase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE1908833A DE1908833C3 (de) 1969-02-21 1969-02-21 Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli

Publications (3)

Publication Number Publication Date
DE1908833A1 DE1908833A1 (de) 1970-09-24
DE1908833B2 DE1908833B2 (de) 1973-11-29
DE1908833C3 true DE1908833C3 (de) 1974-06-27

Family

ID=5725973

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1908833A Expired DE1908833C3 (de) 1969-02-21 1969-02-21 Verfahren zur Anreicherung und Reinigung von L-Asparaginase aus einem Filtrat einer Mangansalzfällung eines Extraktes von Zellen von Escherichia coli

Country Status (3)

Country Link
US (1) US3684659A (enExample)
DE (1) DE1908833C3 (enExample)
GB (1) GB1231982A (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4729957A (en) * 1984-12-13 1988-03-08 The United States Of America As Represented By The Department Of Health And Human Services Process for manufacture of L-asparaginase from erwinia chrysanthemi
WO1987003618A1 (en) * 1985-12-13 1987-06-18 United States Of America, Represented By The Unite Process for manufacture of l-asparaginase from erwinia carotovora
DE102012001633A1 (de) 2012-01-30 2013-08-01 Kolbenschmidt Pierburg Innovations Gmbh Mechanisch steuerbare Ventiltriebanordnung

Also Published As

Publication number Publication date
US3684659A (en) 1972-08-15
DE1908833A1 (de) 1970-09-24
GB1231982A (enExample) 1971-05-12
DE1908833B2 (de) 1973-11-29

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Legal Events

Date Code Title Description
C3 Grant after two publication steps (3rd publication)