DE1202438B - Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen Koerperfluessigkeiten - Google Patents
Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen KoerperfluessigkeitenInfo
- Publication number
- DE1202438B DE1202438B DEB74932A DEB0074932A DE1202438B DE 1202438 B DE1202438 B DE 1202438B DE B74932 A DEB74932 A DE B74932A DE B0074932 A DEB0074932 A DE B0074932A DE 1202438 B DE1202438 B DE 1202438B
- Authority
- DE
- Germany
- Prior art keywords
- plasmin
- human
- trypsin inhibitors
- inhibitors
- body fluids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 8
- 229940012957 plasmin Drugs 0.000 title claims description 8
- 239000002806 plasmin inhibitor Substances 0.000 title claims description 7
- 239000002753 trypsin inhibitor Substances 0.000 title claims description 7
- 241001465754 Metazoa Species 0.000 title claims description 4
- 210000001124 body fluid Anatomy 0.000 title claims description 4
- 239000010839 body fluid Substances 0.000 title claims description 4
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- -1 carboxymethyl polysaccharides Chemical class 0.000 claims description 6
- 150000001450 anions Chemical class 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 239000000337 buffer salt Substances 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 description 19
- 210000002966 serum Anatomy 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000007979 citrate buffer Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 229920002307 Dextran Polymers 0.000 description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229920000669 heparin Polymers 0.000 description 4
- 229960002897 heparin Drugs 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 229920000447 polyanionic polymer Polymers 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000006167 equilibration buffer Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- IYLLULUTZPKQBW-UHFFFAOYSA-N Acrinol Chemical compound CC(O)C(O)=O.C1=C(N)C=CC2=C(N)C3=CC(OCC)=CC=C3N=C21 IYLLULUTZPKQBW-UHFFFAOYSA-N 0.000 description 1
- 208000009663 Acute Necrotizing Pancreatitis Diseases 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 108010088842 Fibrinolysin Proteins 0.000 description 1
- 229920001499 Heparinoid Polymers 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 206010058096 Pancreatic necrosis Diseases 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000002554 heparinoid Substances 0.000 description 1
- 229940025770 heparinoids Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 150000003460 sulfonic acids Chemical class 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEB74932A DE1202438B (de) | 1964-01-10 | 1964-01-10 | Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen Koerperfluessigkeiten |
| AT807164A AT258468B (de) | 1964-01-10 | 1964-09-21 | Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen Körperflüssigkeiten |
| NL6411025A NL6411025A (enrdf_load_stackoverflow) | 1964-01-10 | 1964-09-22 | |
| GB122065A GB1083822A (en) | 1964-01-10 | 1965-01-11 | Method for preparing plasmin and trypsin inhibitors from human or animal body fluids |
| FR1433A FR1434199A (fr) | 1964-01-10 | 1965-01-11 | Procédé d'isolement d'inhibiteurs de la plasmine et de la trypsine à partir de liquides organiques d'origine humaine ou animale |
| BE658139D BE658139A (enrdf_load_stackoverflow) | 1964-01-10 | 1965-01-11 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEB74932A DE1202438B (de) | 1964-01-10 | 1964-01-10 | Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen Koerperfluessigkeiten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE1202438B true DE1202438B (de) | 1965-10-07 |
Family
ID=6978463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEB74932A Pending DE1202438B (de) | 1964-01-10 | 1964-01-10 | Verfahren zur Gewinnung von Plasmin- und Trypsin-Inhibitoren aus menschlichen und tierischen Koerperfluessigkeiten |
Country Status (5)
| Country | Link |
|---|---|
| AT (1) | AT258468B (enrdf_load_stackoverflow) |
| BE (1) | BE658139A (enrdf_load_stackoverflow) |
| DE (1) | DE1202438B (enrdf_load_stackoverflow) |
| GB (1) | GB1083822A (enrdf_load_stackoverflow) |
| NL (1) | NL6411025A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4330622A (en) * | 1979-09-12 | 1982-05-18 | Becton Dickinson & Co. | Elimination of non-microbial turbidity in culture media |
-
1964
- 1964-01-10 DE DEB74932A patent/DE1202438B/de active Pending
- 1964-09-21 AT AT807164A patent/AT258468B/de active
- 1964-09-22 NL NL6411025A patent/NL6411025A/xx unknown
-
1965
- 1965-01-11 BE BE658139D patent/BE658139A/xx unknown
- 1965-01-11 GB GB122065A patent/GB1083822A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4330622A (en) * | 1979-09-12 | 1982-05-18 | Becton Dickinson & Co. | Elimination of non-microbial turbidity in culture media |
Also Published As
| Publication number | Publication date |
|---|---|
| GB1083822A (en) | 1967-09-20 |
| BE658139A (enrdf_load_stackoverflow) | 1965-07-12 |
| AT258468B (de) | 1967-11-27 |
| NL6411025A (enrdf_load_stackoverflow) | 1965-07-12 |
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