DE10130155B3 - Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat - Google Patents
Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat Download PDFInfo
- Publication number
- DE10130155B3 DE10130155B3 DE10130155A DE10130155A DE10130155B3 DE 10130155 B3 DE10130155 B3 DE 10130155B3 DE 10130155 A DE10130155 A DE 10130155A DE 10130155 A DE10130155 A DE 10130155A DE 10130155 B3 DE10130155 B3 DE 10130155B3
- Authority
- DE
- Germany
- Prior art keywords
- reporter gene
- gene construct
- rev
- hiv
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108700008625 Reporter Genes Proteins 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 15
- 108010070875 Human Immunodeficiency Virus tat Gene Products Proteins 0.000 title abstract description 6
- 210000004027 cell Anatomy 0.000 claims abstract description 56
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 23
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 23
- 230000000694 effects Effects 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 8
- 230000001413 cellular effect Effects 0.000 claims abstract description 6
- 108020001507 fusion proteins Proteins 0.000 claims abstract description 6
- 102000037865 fusion proteins Human genes 0.000 claims abstract description 6
- 230000027455 binding Effects 0.000 claims abstract description 5
- 210000003855 cell nucleus Anatomy 0.000 claims abstract description 5
- 230000003612 virological effect Effects 0.000 claims abstract description 5
- 230000014509 gene expression Effects 0.000 claims description 19
- 101710149951 Protein Tat Proteins 0.000 claims description 18
- 238000012360 testing method Methods 0.000 claims description 16
- 108700004025 env Genes Proteins 0.000 claims description 14
- 108091006047 fluorescent proteins Proteins 0.000 claims description 9
- 102000034287 fluorescent proteins Human genes 0.000 claims description 9
- 230000014616 translation Effects 0.000 claims description 8
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 5
- 239000013604 expression vector Substances 0.000 claims description 5
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 5
- 238000001243 protein synthesis Methods 0.000 claims description 4
- 108010054624 red fluorescent protein Proteins 0.000 claims description 4
- 238000013518 transcription Methods 0.000 claims description 4
- 230000035897 transcription Effects 0.000 claims description 4
- 239000013613 expression plasmid Substances 0.000 claims description 3
- 230000008488 polyadenylation Effects 0.000 claims description 3
- 238000000799 fluorescence microscopy Methods 0.000 claims description 2
- 238000000338 in vitro Methods 0.000 claims description 2
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
- 210000001671 embryonic stem cell Anatomy 0.000 claims 1
- 210000000805 cytoplasm Anatomy 0.000 abstract description 6
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 238000010998 test method Methods 0.000 abstract description 3
- 241000725303 Human immunodeficiency virus Species 0.000 description 15
- 108020004999 messenger RNA Proteins 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000001890 transfection Methods 0.000 description 5
- 238000013519 translation Methods 0.000 description 4
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 3
- 208000031886 HIV Infections Diseases 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 208000037357 HIV infectious disease Diseases 0.000 description 2
- 108010048209 Human Immunodeficiency Virus Proteins Proteins 0.000 description 2
- 101710150344 Protein Rev Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 108010021843 fluorescent protein 583 Proteins 0.000 description 2
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 101710177291 Gag polyprotein Proteins 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 101710130262 Probable Vpr-like protein Proteins 0.000 description 1
- BGNQYGRXEXDAIQ-UHFFFAOYSA-N Pyrazosulfuron-ethyl Chemical compound C1=NN(C)C(S(=O)(=O)NC(=O)NC=2N=C(OC)C=C(OC)N=2)=C1C(=O)OCC BGNQYGRXEXDAIQ-UHFFFAOYSA-N 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 101150047356 dec-1 gene Proteins 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 108700004026 gag Genes Proteins 0.000 description 1
- 101150098622 gag gene Proteins 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 102000037983 regulatory factors Human genes 0.000 description 1
- 108091008025 regulatory factors Proteins 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000005029 transcription elongation Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10130155A DE10130155B3 (de) | 2001-06-22 | 2001-06-22 | Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat |
| US10/176,010 US7183047B2 (en) | 2001-06-22 | 2002-06-20 | Reporter gene construct for the detection of HIV Rev and HIV Tat |
| JP2002179815A JP2003111587A (ja) | 2001-06-22 | 2002-06-20 | ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物 |
| EP02013820A EP1270744A1 (en) | 2001-06-22 | 2002-06-21 | Reporter gene construct for the detection of HIV rev and HIV tat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10130155A DE10130155B3 (de) | 2001-06-22 | 2001-06-22 | Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10130155B3 true DE10130155B3 (de) | 2004-03-04 |
Family
ID=7689082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10130155A Expired - Fee Related DE10130155B3 (de) | 2001-06-22 | 2001-06-22 | Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7183047B2 (enExample) |
| EP (1) | EP1270744A1 (enExample) |
| JP (1) | JP2003111587A (enExample) |
| DE (1) | DE10130155B3 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10053781B4 (de) * | 2000-10-30 | 2008-07-03 | Geneart Ag | Kernexportreportersystem |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1886417A (zh) * | 2003-09-28 | 2006-12-27 | 美国卫生与公共服务部 | Hiv－依赖型表现构筑体及其用途 |
| CA2679511A1 (en) * | 2007-03-16 | 2008-09-25 | 454 Life Sciences Corporation | System and method for detection of hiv drug resistant variants |
| WO2015023811A2 (en) | 2013-08-16 | 2015-02-19 | The J. David Gladstone Institutes | Compositions and methods for identifying latently infected cells |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997009342A1 (en) * | 1995-09-08 | 1997-03-13 | Scriptgen Pharmaceuticals, Inc. | Screen for compounds with affinity for rna |
| US5654398A (en) * | 1993-06-03 | 1997-08-05 | The Regents Of The University Of California | Compositions and methods for inhibiting replication of human immunodeficiency virus-1 |
| WO1998044147A1 (en) * | 1997-04-01 | 1998-10-08 | The Regents Of The University Of California | Screening methods in eucaryotic cells |
| WO1999039011A1 (en) * | 1998-01-30 | 1999-08-05 | Duke University | A method of assaying for rna:protein interactions |
| WO1999064625A2 (en) * | 1998-06-05 | 1999-12-16 | Ribotargets Limited | Methods and kits for discovery of rna-binding compounds |
| US6225045B1 (en) * | 1996-05-13 | 2001-05-01 | Ribotargets, Ltd. | Assays for screening for inhibitors of HIV |
-
2001
- 2001-06-22 DE DE10130155A patent/DE10130155B3/de not_active Expired - Fee Related
-
2002
- 2002-06-20 US US10/176,010 patent/US7183047B2/en not_active Expired - Fee Related
- 2002-06-20 JP JP2002179815A patent/JP2003111587A/ja active Pending
- 2002-06-21 EP EP02013820A patent/EP1270744A1/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5654398A (en) * | 1993-06-03 | 1997-08-05 | The Regents Of The University Of California | Compositions and methods for inhibiting replication of human immunodeficiency virus-1 |
| WO1997009342A1 (en) * | 1995-09-08 | 1997-03-13 | Scriptgen Pharmaceuticals, Inc. | Screen for compounds with affinity for rna |
| US6225045B1 (en) * | 1996-05-13 | 2001-05-01 | Ribotargets, Ltd. | Assays for screening for inhibitors of HIV |
| WO1998044147A1 (en) * | 1997-04-01 | 1998-10-08 | The Regents Of The University Of California | Screening methods in eucaryotic cells |
| WO1999039011A1 (en) * | 1998-01-30 | 1999-08-05 | Duke University | A method of assaying for rna:protein interactions |
| WO1999064625A2 (en) * | 1998-06-05 | 1999-12-16 | Ribotargets Limited | Methods and kits for discovery of rna-binding compounds |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10053781B4 (de) * | 2000-10-30 | 2008-07-03 | Geneart Ag | Kernexportreportersystem |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1270744A1 (en) | 2003-01-02 |
| US7183047B2 (en) | 2007-02-27 |
| JP2003111587A (ja) | 2003-04-15 |
| US20030092032A1 (en) | 2003-05-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8100 | Publication of patent without earlier publication of application | ||
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: HELMHOLTZ ZENTRUM MUENCHEN DEUTSCHES FORSCHUNG, DE |
|
| R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |