JP2003111587A - ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物 - Google Patents

ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物

Info

Publication number
JP2003111587A
JP2003111587A JP2002179815A JP2002179815A JP2003111587A JP 2003111587 A JP2003111587 A JP 2003111587A JP 2002179815 A JP2002179815 A JP 2002179815A JP 2002179815 A JP2002179815 A JP 2002179815A JP 2003111587 A JP2003111587 A JP 2003111587A
Authority
JP
Japan
Prior art keywords
reporter gene
rev
gene construct
tat
reporter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2002179815A
Other languages
English (en)
Japanese (ja)
Other versions
JP2003111587A5 (enExample
Inventor
Ruth Brack-Werner
ブラック−ヴェルナー ルート
Markus Neumann
ノイマン マルクス
Horst Wolf
ヴォルフ ホルスト
Volker Erfle
エルフレ フォルカー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gsf Forschungszentrum fur Umwelt & Gesundheit GmbH
Helmholtz Zentrum München
Original Assignee
Gsf Forschungszentrum fur Umwelt & Gesundheit GmbH
Helmholtz Zentrum München
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gsf Forschungszentrum fur Umwelt & Gesundheit GmbH, Helmholtz Zentrum München filed Critical Gsf Forschungszentrum fur Umwelt & Gesundheit GmbH
Publication of JP2003111587A publication Critical patent/JP2003111587A/ja
Publication of JP2003111587A5 publication Critical patent/JP2003111587A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2002179815A 2001-06-22 2002-06-20 ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物 Pending JP2003111587A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10130155A DE10130155B3 (de) 2001-06-22 2001-06-22 Reportergen-Konstrukt zum Nachweis von HIV-Rev und HIV-Tat
DE10130155.3 2001-06-22

Publications (2)

Publication Number Publication Date
JP2003111587A true JP2003111587A (ja) 2003-04-15
JP2003111587A5 JP2003111587A5 (enExample) 2005-09-29

Family

ID=7689082

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2002179815A Pending JP2003111587A (ja) 2001-06-22 2002-06-20 ＨＩＶＲｅｖおよびＨＩＶＴａｔを検出するためのレポーター遺伝子構築物

Country Status (4)

Country Link
US (1) US7183047B2 (enExample)
EP (1) EP1270744A1 (enExample)
JP (1) JP2003111587A (enExample)
DE (1) DE10130155B3 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10053781B4 (de) * 2000-10-30 2008-07-03 Geneart Ag Kernexportreportersystem
WO2005033280A2 (en) * 2003-09-28 2005-04-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Hiv-dependent expression constructs and uses therefor
EP2121984A2 (en) * 2007-03-16 2009-11-25 454 Life Sciences Corporation System and method for detection of hiv drug resistant variants
EP3033087A4 (en) 2013-08-16 2017-03-15 The J. David Gladstone Institutes Compositions and methods for identifying latently infected cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654398A (en) * 1993-06-03 1997-08-05 The Regents Of The University Of California Compositions and methods for inhibiting replication of human immunodeficiency virus-1
CA2203832A1 (en) * 1995-09-08 1997-03-13 Jaime E. Arenas Screen for compounds with affinity for rna
US6225045B1 (en) * 1996-05-13 2001-05-01 Ribotargets, Ltd. Assays for screening for inhibitors of HIV
US5989814A (en) * 1997-04-01 1999-11-23 Reagents Of The University Of California Screening methods in eucaryotic cells
WO1999039011A1 (en) * 1998-01-30 1999-08-05 Duke University A method of assaying for rna:protein interactions
US6573045B1 (en) * 1998-06-05 2003-06-03 Ribotargets, Ltd. Methods and kits for discovery of RNA-binding compounds

Also Published As

Publication number Publication date
DE10130155B3 (de) 2004-03-04
US20030092032A1 (en) 2003-05-15
EP1270744A1 (en) 2003-01-02
US7183047B2 (en) 2007-02-27

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