DD208824A5 - Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden - Google Patents

Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden Download PDF

Info

Publication number
DD208824A5
DD208824A5 DD83247040A DD24704083A DD208824A5 DD 208824 A5 DD208824 A5 DD 208824A5 DD 83247040 A DD83247040 A DD 83247040A DD 24704083 A DD24704083 A DD 24704083A DD 208824 A5 DD208824 A5 DD 208824A5
Authority
DD
German Democratic Republic
Prior art keywords
cholesterol
pseudomonas
buffer
phosphate
enzyme
Prior art date
Application number
DD83247040A
Other languages
German (de)
English (en)
Inventor
Herbert Buschek
Helmut Schlumberger
Joachim Siedel
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of DD208824A5 publication Critical patent/DD208824A5/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DD83247040A 1982-01-07 1983-01-05 Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden DD208824A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823200274 DE3200274A1 (de) 1982-01-07 1982-01-07 Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden

Publications (1)

Publication Number Publication Date
DD208824A5 true DD208824A5 (de) 1984-04-11

Family

ID=6152717

Family Applications (1)

Application Number Title Priority Date Filing Date
DD83247040A DD208824A5 (de) 1982-01-07 1983-01-05 Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden

Country Status (12)

Country Link
US (1) US4476223A (ja)
EP (1) EP0084684B1 (ja)
JP (1) JPS58175488A (ja)
AT (1) ATE12659T1 (ja)
AU (1) AU553593B2 (ja)
CA (1) CA1198698A (ja)
DD (1) DD208824A5 (ja)
DE (2) DE3200274A1 (ja)
DK (1) DK163527C (ja)
ES (1) ES8402347A1 (ja)
FI (1) FI77262C (ja)
ZA (1) ZA8367B (ja)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6030680A (ja) * 1983-07-30 1985-02-16 Amano Pharmaceut Co Ltd リパ−ゼにエステラ−ゼ活性を付加する方法
DE3447390A1 (de) * 1984-12-24 1986-07-03 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur gewinnung von cholesterinesterase
EP0218083A1 (en) * 1985-09-03 1987-04-15 Abbott Laboratories Stabilized cholesterol reagent and method for determining total cholesterol using the reagent
DK145090D0 (da) * 1990-06-14 1990-06-14 Novo Nordisk As Cellulasepraeparat og anvendelse deraf
US5460944A (en) * 1991-10-28 1995-10-24 Boehringer Mannheim Gmbh Storable protein solution
DK99492D0 (da) * 1992-08-07 1992-08-07 Novo Nordisk As Nyt enzym
EP3722418A1 (en) * 2019-04-08 2020-10-14 AB Enzymes Oy Solution stable enzyme composition

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR891187A (fr) * 1942-09-25 1944-02-29 Procédé de conservation de la présure
US3884764A (en) * 1974-03-25 1975-05-20 Eastman Kodak Co Method and composition for blood serum cholesterol analysis
DE2911284C2 (de) * 1979-03-22 1982-01-28 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagenz zur Aktivierung der Cholesterinesterase
DE2933648A1 (de) * 1979-08-20 1981-03-26 Boehringer Mannheim Gmbh, 68305 Mannheim Verfahren zur gewinnung von cholesterinesterase
DE2933646A1 (de) * 1979-08-20 1981-03-26 Boehringer Mannheim Gmbh, 68305 Mannheim Verfahren zur gewinnung von cholesterinesterase
US4409326A (en) * 1980-07-10 1983-10-11 Modrovich Ivan Endre Stabilized enzymatic solutions and method for determining total cholesterol in human serum

Also Published As

Publication number Publication date
JPS58175488A (ja) 1983-10-14
EP0084684A1 (de) 1983-08-03
ATE12659T1 (de) 1985-04-15
AU553593B2 (en) 1986-07-24
DE3263043D1 (en) 1985-05-15
JPS6121076B2 (ja) 1986-05-24
FI830036A0 (fi) 1983-01-06
CA1198698A (en) 1985-12-31
ES518694A0 (es) 1984-02-16
DE3200274A1 (de) 1983-07-14
DK163527B (da) 1992-03-09
FI77262B (fi) 1988-10-31
DK283D0 (da) 1983-01-03
DK283A (da) 1983-07-08
ZA8367B (en) 1983-10-26
EP0084684B1 (de) 1985-04-10
FI77262C (fi) 1989-02-10
ES8402347A1 (es) 1984-02-16
DK163527C (da) 1992-08-03
FI830036L (fi) 1983-07-08
US4476223A (en) 1984-10-09
AU9166882A (en) 1983-07-14

Similar Documents

Publication Publication Date Title
DE2816229C2 (de) Verfahren und Mittel zur Beseitigung von Trübungen
EP0007058B1 (de) Verfahren und Reagens zur Bestimmung von Glycerin
DE2828658C3 (de) Verfahren zur photometrischen Bestimmung der Untereinheit B der Creatinkinase und Reagens hierfür
DE3537563A1 (de) Stabilisierte zusammensetzung mit einem gehalt an inositoltriphosphat und verfahren zu deren herstellung
DE2316637A1 (de) Verfahren zur bestimmung von cholesterin
DE2904305B1 (de) Reagens zur Lipasebestimmung und Verfahren zu seiner Herstellung
DD155211A5 (de) Verfahren und reagens zur bestimmung von triglyceriden
EP0053692B1 (de) Verfahren und Reagenz zur Bestimmung von Cholesterin
EP0012184A1 (de) Hämolysierlösung, Verwendung einer solchen für die Bestimmung von Glucose im Blut, und Hämolyseverfahren
DE1129003B (de) Diagnostiziermittel zum Nachweis von Glukose in Fluessigkeiten
EP0084684B1 (de) Verfahren zur Stabilisierung wässriger Lösungen von Cholesterinesterase aus Pseudomonaden
DE3342106C2 (de) Komposition für Lipase-Bestimmung
DE3329952A1 (de) Verfahren zur verringerung einer truebung in kontrollseren
DE2512605A1 (de) Verfahren zur bestimmung des gesamt-cholesteringehaltes waessriger fluessigkeiten
DE3006789A1 (de) Verfahren und enzymzusammensetzung zur hydrolyse von glycerinestern
DE69128873T2 (de) Verfahren zur enzymatischen Messung von Wasserstoffperoxid und Reagenz dafür
DE2419444C3 (de) Substrat für ein Enzym
EP0144798B1 (de) Verfahren und Reagenz zur Bestimmung von Glycerin mittels eines kompetitiven Inhibitors für Glycerinkinase
DE69030798T2 (de) Stabilisierung des Glucose-Oxidase-Enzyms in flüssigem Reagenz
DE2911284C2 (de) Verfahren und Reagenz zur Aktivierung der Cholesterinesterase
DE3873437T2 (de) Turbidimetrisches verfahren zum nachweis von lipase in serum.
DE2804356A1 (de) Verfahren zur hydrolyse von protein-gebundenen cholesterinestern
DE2506712C3 (de) Verfahren zur Bestimmung von Cholesterin
DE3246090C2 (ja)
EP0399391B1 (de) Stabilisiertes Reagenzgemisch