CY1112945T1 - Μεθοδοι για την παραγωγη μικροθυλακων τριχων και dε νοvο θηλων και η χρηση τους για ιν viτrο εξετασεις και ιν vivo εμφυτευσεις - Google Patents

Μεθοδοι για την παραγωγη μικροθυλακων τριχων και dε νοvο θηλων και η χρηση τους για ιν viτrο εξετασεις και ιν vivo εμφυτευσεις

Info

Publication number
CY1112945T1
CY1112945T1 CY20121100655T CY121100655T CY1112945T1 CY 1112945 T1 CY1112945 T1 CY 1112945T1 CY 20121100655 T CY20121100655 T CY 20121100655T CY 121100655 T CY121100655 T CY 121100655T CY 1112945 T1 CY1112945 T1 CY 1112945T1
Authority
CY
Cyprus
Prior art keywords
netherlands
methods
different
vivo investigations
hair
Prior art date
Application number
CY20121100655T
Other languages
English (en)
Inventor
Gerd Lindner
Roland Lauster
Original Assignee
Technische Universität Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technische Universität Berlin filed Critical Technische Universität Berlin
Publication of CY1112945T1 publication Critical patent/CY1112945T1/el

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3869—Epithelial tissues other than skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/18—Materials or treatment for tissue regeneration for hair reconstruction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/092—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Vascular Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)

Abstract

Η παρούσα εφεύρεση αφορά μια μέθοδο για την παραγωγή μικροθυλάκων τριχών η οποία περιλαμβάνει τα στάδια: α) παροχής de novo θηλών, β) παροχής άλλων κυτταρικών πληθυσμών που επιλέγονται από την ομάδα ινοβλαστών, κερατινοκυττάρων και μελανοκυττάρων, και συγκαλλιέργειας των de novo θηλών με τουλάχιστον έναν άλλο κυτταρικό πληθυσμό σε μη προσφυόμενα δοχεία καλλιέργειας. Η παρούσα εφεύρεση αφορά επίσης μεθόδους παραγωγής de novo θηλών χρησιμοποιήσιμων στην εν λόγω μέθοδο για την παραγωγή μικροθυλάκων τριχών.
CY20121100655T 2008-03-28 2012-07-24 Μεθοδοι για την παραγωγη μικροθυλακων τριχων και dε νοvο θηλων και η χρηση τους για ιν viτrο εξετασεις και ιν vivo εμφυτευσεις CY1112945T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08153596A EP2105499A1 (en) 2008-03-28 2008-03-28 Methods for producing de novo papillae and hair microfollicles and their use for in vitro tests and in vivo implantations
EP09724502A EP2274419B1 (en) 2008-03-28 2009-03-23 Methods for producing hair microfollicles and de novo papillae and their use for in vitro tests and in vivo implantations

Publications (1)

Publication Number Publication Date
CY1112945T1 true CY1112945T1 (el) 2016-04-13

Family

ID=39734907

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20121100655T CY1112945T1 (el) 2008-03-28 2012-07-24 Μεθοδοι για την παραγωγη μικροθυλακων τριχων και dε νοvο θηλων και η χρηση τους για ιν viτrο εξετασεις και ιν vivo εμφυτευσεις

Country Status (18)

Country Link
US (2) US8841124B2 (el)
EP (3) EP2105499A1 (el)
JP (1) JP5600671B2 (el)
CN (1) CN102027106B (el)
AT (1) ATE555192T1 (el)
AU (1) AU2009228756B2 (el)
CA (1) CA2719769C (el)
CY (1) CY1112945T1 (el)
DK (1) DK2274419T3 (el)
ES (2) ES2386109T3 (el)
HK (1) HK1156976A1 (el)
HR (1) HRP20120591T1 (el)
HU (1) HUE026065T2 (el)
PL (2) PL2447357T3 (el)
PT (2) PT2274419E (el)
RU (1) RU2507254C2 (el)
SI (1) SI2274419T1 (el)
WO (1) WO2009118283A1 (el)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2483622A (en) * 2009-08-03 2012-03-21 Univ Durham Fibroblast feeder cells
US9321998B2 (en) 2011-02-24 2016-04-26 Organ Technologies Inc. Method of preparing regenerated hair follicle germ for transplantation in which hair color is controlled, composition including regenerated hair follicle germ for transplantation, and method of transplanting regenerated hair follicle germ
JP2012205516A (ja) 2011-03-29 2012-10-25 Osaka Univ 人工皮膚モデルの製造方法、及び人工皮膚モデル
WO2014179559A1 (en) 2013-05-03 2014-11-06 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Skin substitutes and methods for hair follicle neogenesis
FR3008316B1 (fr) * 2013-07-09 2020-01-31 Assistance Publique - Hopitaux De Paris Utilisation de fibroblastes gingivaux dans le traitement de l'alopecie
WO2015066618A1 (en) * 2013-11-01 2015-05-07 The Florida International University Board Of Trustees Context based algorithmic framework for identifying and classifying embedded images of follicle units
SG11201707858TA (en) * 2015-03-30 2017-10-30 Ajinomoto Kk Human serum albumin-containing culture medium for growth of neural stem cells
FR3041656A1 (fr) * 2015-09-29 2017-03-31 Oreal Utilisation des cellules de la matrice pour la preparation d'un microfollicule pileux
DE102015119880B4 (de) 2015-11-17 2018-05-24 Technische Universität Berlin Verfahren zur Herstellung von Haarfollikeln und de novo Papillen sowie deren Verwendung für in vitro Tests und in vivo Implantate
DE102015119877B4 (de) * 2015-11-17 2017-09-21 Technische Universität Berlin Verfahren zur Herstellung eines Hautäquivalents sowie dessen Verwendung für in vitro Tests und in vivo Transplantate
CN105353114B (zh) * 2015-11-18 2017-09-26 广东博溪生物科技有限公司 一种含黑色素的体外测试皮肤模型及其制备方法
FR3054449B1 (fr) * 2016-07-29 2018-08-31 L'oreal Equivalent de peau a compartiments dermiques juxtaposes distincts
FR3061207B1 (fr) 2016-12-23 2023-03-10 Oreal Utilisation des cellules germinales pour la preparation d’un microfollicule pileux
JP2018186719A (ja) * 2017-04-28 2018-11-29 株式会社 バイオミメティクスシンパシーズ 頭皮投与用幹細胞
FR3072972B1 (fr) * 2017-10-30 2023-03-10 Oreal Procede de preparation in vitro d’equivalents de papille dermique et de follicule pileux
CN109468271A (zh) * 2018-12-28 2019-03-15 广州暨大美塑生物科技有限公司 一种皮肤组织来源成纤维细胞快速分离培养方法
CN114085804B (zh) * 2021-11-09 2023-12-26 南方医科大学皮肤病医院（广东省皮肤病医院、广东省皮肤性病防治中心、中国麻风防治研究中心） 一种人胚胎干细胞体外诱导分化形成的皮肤类器官及其高效培养方法与用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10162814B4 (de) 2001-12-19 2005-06-23 Henkel Kgaa Rekonstruiertes Haarfollikelmodell
DE10327879A1 (de) * 2003-06-18 2005-01-05 Henkel Kgaa Rekonstruierte dermale Papille
US20050233450A1 (en) * 2004-01-12 2005-10-20 Goetinck Paul F Methods of inducing hair growth
EP2329833A1 (en) 2004-01-27 2011-06-08 The Hospital for Sick Children Research Institute Methods of making and using skin-derived stem cells
US9375514B2 (en) 2004-05-21 2016-06-28 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Multicellular tissue and organ culture systems

Also Published As

Publication number Publication date
CA2719769C (en) 2018-05-01
CA2719769A1 (en) 2009-10-01
EP2274419B1 (en) 2012-04-25
HUE026065T2 (en) 2016-05-30
RU2507254C2 (ru) 2014-02-20
US9764064B2 (en) 2017-09-19
PT2447357E (pt) 2015-10-23
RU2010144183A (ru) 2012-05-10
CN102027106A (zh) 2011-04-20
EP2447357B1 (en) 2015-07-01
ES2549055T3 (es) 2015-10-22
EP2274419A1 (en) 2011-01-19
DK2274419T3 (da) 2012-07-23
AU2009228756B2 (en) 2014-07-24
US20140370070A1 (en) 2014-12-18
EP2447357A1 (en) 2012-05-02
PL2447357T3 (pl) 2015-12-31
US8841124B2 (en) 2014-09-23
HRP20120591T1 (hr) 2012-08-31
SI2274419T1 (sl) 2012-10-30
ATE555192T1 (de) 2012-05-15
CN102027106B (zh) 2014-05-14
AU2009228756A1 (en) 2009-10-01
WO2009118283A1 (en) 2009-10-01
ES2386109T3 (es) 2012-08-09
EP2105499A1 (en) 2009-09-30
PL2274419T3 (pl) 2012-10-31
HK1156976A1 (en) 2012-06-22
JP5600671B2 (ja) 2014-10-01
JP2011515095A (ja) 2011-05-19
US20110086079A1 (en) 2011-04-14
PT2274419E (pt) 2012-07-06

Similar Documents

Publication Publication Date Title
CY1112945T1 (el) Μεθοδοι για την παραγωγη μικροθυλακων τριχων και dε νοvο θηλων και η χρηση τους για ιν viτrο εξετασεις και ιν vivo εμφυτευσεις
NZ569543A (en) Means and methods for influencing the stability of antibody producing cells
AR111785A2 (es) Método in vitro de producción de eritrocitos
WO2007109223A3 (en) Cell co-culture for hair follicle production
MY159971A (en) Multipotent/pluripotent cells and methods
WO2006050330A3 (en) Platelets from stem cells
CO6531436A2 (es) Métodos de producción de glucoproteínas en cultivos de celulas de mamiferos usando glucocorticoides
EP2650360A3 (en) Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
GB2485112A (en) Differentiation of human embryonic stem cells
RU2012105922A (ru) Дифференцирование человеческих эмбриональных стволовых клеток
BR112012014062A2 (pt) organismo microbiano que não ocorre naturalmente e método para produzir 1,3 bdo
NZ598293A (en) Imaging and evaluating embryos, oocytes, and stem cells
DE112010004990T8 (de) Bipolarplatte für eine Brennstoffzelle und Verfahren zur Herstellung derselben
ATE480615T1 (de) Verfahren zur proliferation von stammzellen
ATE522233T1 (de) Vorbereitung von gewebe für die meniskus- implantation
WO2010020876A3 (en) Cell culture method to form aggregates
CL2012003244A1 (es) Proceso para la produccion de parches o apositos de piel autologa mediante cultivo de queratinocitos y fibroblastos autologos con suero autologo para generacion; parche o apósito que comprende un sustrato de malla sobre la cual se ha colocado colágeno, encima queratinocitos y encima fibroblastos.
WO2013184193A3 (en) Cancer stem cells and methods of using the same
WO2010138873A8 (en) Long term expansion of human hematopoietic stem cells
CL2010000774A1 (es) Medio para el cultivo de celulas de mamiferos que comprende ademas de los nutrientes habituales de los medios de cultivo basales para cultivo de celulas de mamiferos, sobrenadante de la fraccion ii+iii del plasma humano segun el metodo de cohn; procedimiento de preparacion; y su uso.
DE502005006003D1 (de) Verfahren zur herstellung einer epithelzellen enthaltenden zellzusammensetzung
MX2012001004A (es) Proceso para la obtencion de miofibroblastos.
WO2008058942A3 (en) Method of cell culture and method of treatment comprising a vepo protein variant
FI20106354A0 (fi) Menetelmä ihmisen retinan pigmentti-epiteelisolujen valmistamiseksi
RU2006110711A (ru) Эквивалент кожи и способ его получения