CY1110345T1 - Χρηση μιας κυτομετρικης αναλυσης ροης για να βελτιστοποιουνται στρατηγικες καταθεσης κυτταρου για cho κυτταρα - Google Patents

Χρηση μιας κυτομετρικης αναλυσης ροης για να βελτιστοποιουνται στρατηγικες καταθεσης κυτταρου για cho κυτταρα

Info

Publication number
CY1110345T1
CY1110345T1 CY20091100905T CY091100905T CY1110345T1 CY 1110345 T1 CY1110345 T1 CY 1110345T1 CY 20091100905 T CY20091100905 T CY 20091100905T CY 091100905 T CY091100905 T CY 091100905T CY 1110345 T1 CY1110345 T1 CY 1110345T1
Authority
CY
Cyprus
Prior art keywords
production
cho cells
cymometric
flow analysis
thaw
Prior art date
Application number
CY20091100905T
Other languages
English (en)
Inventor
Hitto Kaufmann
Juergen Fieder
Ralf Otto
Original Assignee
Boehringer Ingelheim Pharma Gmbh & Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma Gmbh & Co. Kg filed Critical Boehringer Ingelheim Pharma Gmbh & Co. Kg
Publication of CY1110345T1 publication Critical patent/CY1110345T1/el

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4718—Lipocortins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Παρασκευή των βιοφαρμακευτικών από CHO κύτταρα απαιτεί δημιουργία κύριων-, εργασίας- και μετά την παραγωγή τραπεζών κυττάρου υψηλής ποιότητας, μερικώς υπό GMP συνθήκες. Απαιτείται μία βέλτιστη στρατηγική κρυοσυντήρησης για κάθε νέα παραγωγή κυτταρικής γραμμή, ιδιαιτέρως σε σχέση προς τις επιθυμητές για να θεμελιώνονται μέθοδοι παραγωγής που αποφεύγουν πλήρως ορό ή ακόμη οποιαδήποτε ζωικά συστατικά και για να διασφαλίζεται εύρωστη συμπεριφορά επανατήξης για αξιόπιστη παραγωγή. Εδώ περιγράφουμε μία νέα στρατηγική που χρησιμοποιεί κυτομετρική ανάλυση ροής (FC) των κυττάρων που έχουν υποστεί χρώση με Annexin V για υψηλής απόδοσης χαρακτηρισμό των τραπεζών κυττάρου CHO. Τα δεδομένα μας δείχνουν ότι αυτή η μέθοδος επιτρέπει αξιολόγηση μιας μεθόδου κρυοσυντήρησης μόλις 6 ώρες μετά την επανατήξη.
CY20091100905T 2004-11-10 2009-08-28 Χρηση μιας κυτομετρικης αναλυσης ροης για να βελτιστοποιουνται στρατηγικες καταθεσης κυτταρου για cho κυτταρα CY1110345T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04026673 2004-11-10
EP05803011A EP1820022B1 (en) 2004-11-10 2005-11-07 Use of flow-cytometric analysis to optimize cell banking strategies for cho cells

Publications (1)

Publication Number Publication Date
CY1110345T1 true CY1110345T1 (el) 2015-04-29

Family

ID=36118077

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091100905T CY1110345T1 (el) 2004-11-10 2009-08-28 Χρηση μιας κυτομετρικης αναλυσης ροης για να βελτιστοποιουνται στρατηγικες καταθεσης κυτταρου για cho κυτταρα

Country Status (15)

Country Link
US (2) US7569339B2 (el)
EP (1) EP1820022B1 (el)
JP (1) JP4620739B2 (el)
KR (1) KR101235658B1 (el)
AT (1) ATE433110T1 (el)
CA (1) CA2578400C (el)
CY (1) CY1110345T1 (el)
DE (1) DE602005014805D1 (el)
DK (1) DK1820022T3 (el)
ES (1) ES2325931T3 (el)
PL (1) PL1820022T3 (el)
PT (1) PT1820022E (el)
SI (1) SI1820022T1 (el)
TW (1) TWI364459B (el)
WO (1) WO2006051065A2 (el)

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CA2578400C (en) 2004-11-10 2014-01-14 Boehringer Ingelheim Pharma Gmbh & Co. Kg Use of flow-cytometric analysis to optimize cell banking strategies for cho cells
AU2009314111A1 (en) * 2008-11-12 2010-05-20 Merck Sharp & Dohme Corp. BetaGI-IgG intron for enhanced anti-IGF1 R expression
KR101600733B1 (ko) 2011-10-28 2016-03-09 프로테나 바이오사이언시즈 리미티드 알파-시누클레인을 인식하는 인간화된 항체
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US10501531B2 (en) 2013-03-13 2019-12-10 Prothena Biosciences Limited Tau immunotherapy
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TW201623331A (zh) 2014-03-12 2016-07-01 普羅帝納生物科學公司 抗黑色素瘤細胞黏著分子（ｍｃａｍ）抗體類及使用彼等之相關方法
KR20160131073A (ko) 2014-03-12 2016-11-15 프로테나 바이오사이언시즈 리미티드 Lg4-5에 대해 특이적인 항-라미닌4 항체
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KR102355306B1 (ko) * 2014-07-09 2022-01-24 제넨테크, 인크. 세포 은행의 해동 회수를 개선하는 ph 조정
TWI769570B (zh) 2015-01-28 2022-07-01 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
TWI718122B (zh) 2015-01-28 2021-02-11 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
TWI781507B (zh) 2015-01-28 2022-10-21 愛爾蘭商普羅佘納生物科技有限公司 抗甲狀腺素運送蛋白抗體
CN104833625B (zh) * 2015-06-04 2018-02-23 武汉友芝友生物制药有限公司 表达重组抗体cho细胞系稳定性的早期鉴定方法
CA2998716A1 (en) 2015-09-16 2017-03-23 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of giant cell arteritis, polymyalgia rheumatica or takayasu's arteritis
WO2017046774A2 (en) 2015-09-16 2017-03-23 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of giant cell arteritis, polymyalgia rheumatica or takayasu's arteritis
WO2017149513A1 (en) 2016-03-03 2017-09-08 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
WO2017153953A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
WO2017153955A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
US10752679B2 (en) 2016-05-02 2020-08-25 Prothena Biosciences Limited Tau immunotherapy
WO2017191561A1 (en) 2016-05-02 2017-11-09 Prothena Biosciences Limited Antibodies recognizing tau
PE20190261A1 (es) 2016-05-02 2019-02-25 Prothena Biosciences Ltd Anticuerpos que reconocen tau
WO2017208210A1 (en) 2016-06-03 2017-12-07 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
EP3478714A2 (en) 2016-07-02 2019-05-08 Prothena Biosciences Limited Anti-transthyretin antibodies
EP3478715A2 (en) 2016-07-02 2019-05-08 Prothena Biosciences Limited Anti-transthyretin antibodies
WO2018007924A2 (en) 2016-07-02 2018-01-11 Prothena Biosciences Limited Anti-transthyretin antibodies
PE20200695A1 (es) 2017-05-02 2020-06-16 Prothena Biosciences Ltd Anticuerpos que reconocen tau
CA3076313A1 (en) 2017-09-28 2019-04-04 Prothena Biosciences Limited Dosing regimes for treatment of synucleinopathies
MX2021006134A (es) 2018-11-26 2021-06-23 Forty Seven Inc Anticuerpos humanizados contra c-kit.
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JP2023533458A (ja) 2020-06-24 2023-08-03 プロシーナ バイオサイエンシーズ リミテッド ソルチリンを認識する抗体

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
CA2578400C (en) 2004-11-10 2014-01-14 Boehringer Ingelheim Pharma Gmbh & Co. Kg Use of flow-cytometric analysis to optimize cell banking strategies for cho cells

Also Published As

Publication number Publication date
TWI364459B (en) 2012-05-21
WO2006051065A3 (en) 2006-08-03
US20060121444A1 (en) 2006-06-08
WO2006051065A2 (en) 2006-05-18
EP1820022B1 (en) 2009-06-03
ATE433110T1 (de) 2009-06-15
EP1820022A2 (en) 2007-08-22
JP2008518611A (ja) 2008-06-05
JP4620739B2 (ja) 2011-01-26
KR20070090187A (ko) 2007-09-05
PT1820022E (pt) 2009-08-05
US20100086947A1 (en) 2010-04-08
US7569339B2 (en) 2009-08-04
KR101235658B1 (ko) 2013-02-21
PL1820022T3 (pl) 2009-11-30
CA2578400A1 (en) 2006-05-18
DK1820022T3 (da) 2009-09-14
TW200634161A (en) 2006-10-01
ES2325931T3 (es) 2009-09-24
DE602005014805D1 (de) 2009-07-16
CA2578400C (en) 2014-01-14
SI1820022T1 (sl) 2009-10-31

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