CS205019B2 - Preliminary dyed supporting glass for the blood analysis - Google Patents
Preliminary dyed supporting glass for the blood analysis Download PDFInfo
- Publication number
- CS205019B2 CS205019B2 CS762302A CS230276A CS205019B2 CS 205019 B2 CS205019 B2 CS 205019B2 CS 762302 A CS762302 A CS 762302A CS 230276 A CS230276 A CS 230276A CS 205019 B2 CS205019 B2 CS 205019B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- dyes
- methylene blue
- slide
- blood
- blood analysis
- Prior art date
Links
- 238000004159 blood analysis Methods 0.000 title claims 2
- 239000011521 glass Substances 0.000 title description 6
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 claims abstract description 12
- 239000000975 dye Substances 0.000 claims description 30
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 claims description 13
- 229930003836 cresol Natural products 0.000 claims description 13
- 239000005315 stained glass Substances 0.000 claims description 2
- 150000004682 monohydrates Chemical class 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 10
- 210000004369 blood Anatomy 0.000 abstract description 9
- 239000008280 blood Substances 0.000 abstract description 9
- 239000000203 mixture Substances 0.000 abstract description 6
- 238000000746 purification Methods 0.000 abstract description 5
- 238000011835 investigation Methods 0.000 abstract 1
- VHGXRGXCDVQIKS-KRWDZBQOSA-N methyl (2s)-3-(4-methylphenyl)sulfonyloxy-2-(phenylmethoxycarbonylamino)propanoate Chemical compound C([C@@H](C(=O)OC)NC(=O)OCC=1C=CC=CC=1)OS(=O)(=O)C1=CC=C(C)C=C1 VHGXRGXCDVQIKS-KRWDZBQOSA-N 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000013078 crystal Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000004809 thin layer chromatography Methods 0.000 description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical group [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 229960000907 methylthioninium chloride Drugs 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- -1 N-ethylamino Chemical group 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- NZYCYASKVWSANA-UHFFFAOYSA-M new methylene blue Chemical compound [Cl-].CCNC1=C(C)C=C2N=C(C=C(C(NCC)=C3)C)C3=[S+]C2=C1 NZYCYASKVWSANA-UHFFFAOYSA-M 0.000 description 2
- 210000001995 reticulocyte Anatomy 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- DLYUQMMRRRQYAE-UHFFFAOYSA-N tetraphosphorus decaoxide Chemical compound O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- 235000005074 zinc chloride Nutrition 0.000 description 2
- MHOFGBJTSNWTDT-UHFFFAOYSA-M 2-[n-ethyl-4-[(6-methoxy-3-methyl-1,3-benzothiazol-3-ium-2-yl)diazenyl]anilino]ethanol;methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC(N(CCO)CC)=CC=C1N=NC1=[N+](C)C2=CC=C(OC)C=C2S1 MHOFGBJTSNWTDT-UHFFFAOYSA-M 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000012932 acetate dye Substances 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 125000000853 cresyl group Chemical group C1(=CC=C(C=C1)C)* 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- IJKVHSBPTUYDLN-UHFFFAOYSA-N dihydroxy(oxo)silane Chemical compound O[Si](O)=O IJKVHSBPTUYDLN-UHFFFAOYSA-N 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 210000000224 granular leucocyte Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 235000010483 polyoxyethylene sorbitan monopalmitate Nutrition 0.000 description 1
- 239000000249 polyoxyethylene sorbitan monopalmitate Substances 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Microscoopes, Condenser (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2515966A DE2515966C3 (de) | 1975-04-11 | 1975-04-11 | Vorgefärbte Objektträger für die Blutunte rsuchung |
Publications (1)
Publication Number | Publication Date |
---|---|
CS205019B2 true CS205019B2 (en) | 1981-04-30 |
Family
ID=5943661
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CS762302A CS205019B2 (en) | 1975-04-11 | 1976-04-07 | Preliminary dyed supporting glass for the blood analysis |
Country Status (23)
Country | Link |
---|---|
US (1) | US4070495A (xx) |
JP (1) | JPS51126893A (xx) |
AR (1) | AR210757A1 (xx) |
AT (1) | AT350190B (xx) |
BE (1) | BE840454A (xx) |
CA (1) | CA1052675A (xx) |
CH (1) | CH621629A5 (xx) |
CS (1) | CS205019B2 (xx) |
DD (1) | DD123628A5 (xx) |
DE (1) | DE2515966C3 (xx) |
DK (1) | DK145318C (xx) |
ES (1) | ES446836A1 (xx) |
FI (1) | FI59677C (xx) |
FR (1) | FR2307271A1 (xx) |
GB (1) | GB1480576A (xx) |
HU (1) | HU176928B (xx) |
IE (1) | IE42552B1 (xx) |
IT (1) | IT1059617B (xx) |
LU (1) | LU74738A1 (xx) |
NL (1) | NL7603628A (xx) |
PL (1) | PL107576B1 (xx) |
SE (1) | SE420355B (xx) |
SU (1) | SU904536A3 (xx) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2651060C3 (de) * | 1976-11-09 | 1980-06-26 | Boehringer Mannheim Gmbh, 6800 Mannheim | Differential-diagnostische Spermauntersuchung |
US4193980A (en) * | 1978-01-05 | 1980-03-18 | Corning Glass Works | Dry preparation for reticulocyte staining |
US4248821A (en) * | 1979-07-25 | 1981-02-03 | Dellen Adrian F Van | Method and device for embedding a specimen for microscopic examination |
US4400370A (en) * | 1980-03-12 | 1983-08-23 | Lawrence Kass | Metachromatic dye sorption means for differential determination of leukocytes |
US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
CA1155041A (en) * | 1980-04-21 | 1983-10-11 | Michael E. Jolley | Fluorescent nucleic acid stains |
US4500509A (en) * | 1981-03-11 | 1985-02-19 | Lawrence Kass | Metachromatic dye sorption and fluorescent light emmisive means for differential determination of developmental stages of neutrophilic granulocytic cells and other leukocytes |
US4606950A (en) * | 1984-08-31 | 1986-08-19 | Ellen M. Corbet | Method for assembling a floral arrangement |
US5106744A (en) * | 1990-11-01 | 1992-04-21 | Cytocolor Inc. | Method of staining monocytes and compositions thereof |
ES2736160T3 (es) | 2004-09-23 | 2019-12-26 | Wista Lab Ltd | Procedimientos de síntesis química y purificación de compuestos de diaminofenotiazinio que incluyen cloruro de metiltioninio (CMT) |
PL2057136T3 (pl) | 2006-07-11 | 2013-10-31 | Wista Lab Ltd | Sposoby syntezy i/lub oczyszczania związków diaminofenotiazynowych |
WO2019084513A1 (en) * | 2017-10-26 | 2019-05-02 | Essenlix Corporation | QUICK MEASUREMENT OF PLATELETS |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT966513B (it) * | 1970-10-30 | 1974-02-20 | Gen Electric | Vetrini precolorati per esami del sangue |
US3796594A (en) * | 1970-10-30 | 1974-03-12 | Gen Electric | Stain coated slides for differentially staining blood |
US3906120A (en) * | 1970-10-30 | 1975-09-16 | Gen Electric | Method for preparing slides for blood evaluation |
GB1473945A (en) * | 1973-05-24 | 1977-05-18 | Gen Electric | Method for preparing slides for blood evaluation |
-
1975
- 1975-04-11 DE DE2515966A patent/DE2515966C3/de not_active Expired
-
1976
- 1976-02-27 SE SE7602728A patent/SE420355B/xx unknown
- 1976-03-31 DK DK149876A patent/DK145318C/da not_active IP Right Cessation
- 1976-03-31 US US05/672,572 patent/US4070495A/en not_active Expired - Lifetime
- 1976-04-01 CA CA249,401A patent/CA1052675A/en not_active Expired
- 1976-04-05 AR AR262783A patent/AR210757A1/es active
- 1976-04-05 HU HU76BO1607A patent/HU176928B/hu unknown
- 1976-04-05 FI FI760911A patent/FI59677C/fi not_active IP Right Cessation
- 1976-04-06 DD DD192223A patent/DD123628A5/xx unknown
- 1976-04-06 GB GB13853/76A patent/GB1480576A/en not_active Expired
- 1976-04-07 IT IT22060/76A patent/IT1059617B/it active
- 1976-04-07 BE BE165900A patent/BE840454A/xx not_active IP Right Cessation
- 1976-04-07 NL NL7603628A patent/NL7603628A/xx not_active Application Discontinuation
- 1976-04-07 CS CS762302A patent/CS205019B2/cs unknown
- 1976-04-07 CH CH439376A patent/CH621629A5/de not_active IP Right Cessation
- 1976-04-08 SU SU762346753A patent/SU904536A3/ru active
- 1976-04-09 FR FR7610487A patent/FR2307271A1/fr active Granted
- 1976-04-09 ES ES446836A patent/ES446836A1/es not_active Expired
- 1976-04-09 IE IE752/76A patent/IE42552B1/en unknown
- 1976-04-09 AT AT262076A patent/AT350190B/de not_active IP Right Cessation
- 1976-04-09 LU LU74738A patent/LU74738A1/xx unknown
- 1976-04-10 PL PL1976188674A patent/PL107576B1/pl unknown
- 1976-04-12 JP JP51041173A patent/JPS51126893A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
NL7603628A (nl) | 1976-10-13 |
HU176928B (hu) | 1981-06-28 |
AT350190B (de) | 1979-05-10 |
DE2515966C3 (de) | 1979-11-22 |
LU74738A1 (xx) | 1976-11-11 |
AR210757A1 (es) | 1977-09-15 |
AU1267776A (en) | 1977-10-13 |
DK145318C (da) | 1983-04-05 |
DE2515966A1 (de) | 1976-10-21 |
PL107576B1 (pl) | 1980-02-29 |
ES446836A1 (es) | 1977-06-01 |
GB1480576A (en) | 1977-07-20 |
FR2307271B1 (xx) | 1980-03-28 |
DE2515966B2 (de) | 1979-03-22 |
FI760911A (xx) | 1976-10-12 |
FI59677B (fi) | 1981-05-29 |
FI59677C (fi) | 1981-09-10 |
SU904536A3 (ru) | 1982-02-07 |
DK145318B (da) | 1982-10-25 |
DD123628A5 (xx) | 1977-01-05 |
ATA262076A (de) | 1978-10-15 |
CH621629A5 (xx) | 1981-02-13 |
BE840454A (fr) | 1976-10-07 |
CA1052675A (en) | 1979-04-17 |
FR2307271A1 (fr) | 1976-11-05 |
DK149876A (da) | 1976-10-12 |
IT1059617B (it) | 1982-06-21 |
IE42552L (en) | 1976-10-11 |
US4070495A (en) | 1978-01-24 |
SE420355B (sv) | 1981-09-28 |
SE7602728L (sv) | 1976-10-12 |
JPS51126893A (en) | 1976-11-05 |
IE42552B1 (en) | 1980-08-27 |
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