CS133191A3 - Process for preparing hirudin and hirudin polypeptide - Google Patents

Process for preparing hirudin and hirudin polypeptide Download PDF

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Publication number
CS133191A3
CS133191A3 CS911331A CS133191A CS133191A3 CS 133191 A3 CS133191 A3 CS 133191A3 CS 911331 A CS911331 A CS 911331A CS 133191 A CS133191 A CS 133191A CS 133191 A3 CS133191 A3 CS 133191A3
Authority
CS
Czechoslovakia
Prior art keywords
gly
hirudin
polypeptide
glu
expression vector
Prior art date
Application number
CS911331A
Other languages
Czech (cs)
English (en)
Inventor
Luca Benatti
Paolo Carminati
Jacqueline Lansen
Guy Mazue
Romeo Roucucci
Paolo Sarmientos
Emanuela Scacheri
Taxis Philippe De
Original Assignee
Erba Carlo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Erba Carlo Spa filed Critical Erba Carlo Spa
Publication of CS133191A3 publication Critical patent/CS133191A3/cs

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CS911331A 1990-05-10 1991-05-07 Process for preparing hirudin and hirudin polypeptide CS133191A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909010552A GB9010552D0 (en) 1990-05-10 1990-05-10 Method for the recombinant production of hirudins and novel hirudins

Publications (1)

Publication Number Publication Date
CS133191A3 true CS133191A3 (en) 1992-01-15

Family

ID=10675801

Family Applications (1)

Application Number Title Priority Date Filing Date
CS911331A CS133191A3 (en) 1990-05-10 1991-05-07 Process for preparing hirudin and hirudin polypeptide

Country Status (16)

Country Link
EP (1) EP0482144A1 (no)
JP (1) JPH05500615A (no)
KR (1) KR920703817A (no)
CN (1) CN1057294A (no)
AU (1) AU7652591A (no)
CA (1) CA2063575A1 (no)
CS (1) CS133191A3 (no)
FI (1) FI920058A0 (no)
GB (1) GB9010552D0 (no)
HU (1) HUT59961A (no)
IE (1) IE911578A1 (no)
IL (1) IL98074A0 (no)
NO (1) NO920116L (no)
PT (1) PT97604A (no)
WO (1) WO1991017250A1 (no)
ZA (1) ZA913503B (no)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE176500T1 (de) * 1990-11-08 1999-02-15 Japan Energy Corp Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
US5972648A (en) * 1993-09-28 1999-10-26 Japan Energy Corporation Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients
DE102005002978B4 (de) * 2005-01-21 2013-04-25 Merz Pharma Gmbh & Co. Kgaa Rekombinante Expression von Proteinen in einer disulfidverbrückten, zweikettigen Form
DK2667878T3 (en) 2011-01-25 2016-06-27 Univ Catholique Louvain Configurations and methods for cell transplantation
WO2013110354A1 (en) 2012-01-25 2013-08-01 Université Catholique de Louvain Compositions and methods for cell transplantation
CN108220369B (zh) * 2017-12-04 2021-03-12 广东双骏生物科技有限公司 一种生产重组水蛭素的方法
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法
CN115677850B (zh) * 2021-07-24 2024-03-08 王大勇 具备较强抗凝血活性的医蛭基因突变水蛭素及其制备方法
CN114736289B (zh) * 2022-03-17 2023-07-18 华南理工大学 一种带有酪氨酸硫酸化修饰的水蛭素的化学合成方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins

Also Published As

Publication number Publication date
HUT59961A (en) 1992-07-28
EP0482144A1 (en) 1992-04-29
IL98074A0 (en) 1992-06-21
AU7652591A (en) 1991-11-27
NO920116D0 (no) 1992-01-09
KR920703817A (ko) 1992-12-18
GB9010552D0 (en) 1990-07-04
IE911578A1 (en) 1991-11-20
PT97604A (pt) 1992-02-28
ZA913503B (en) 1992-02-26
HU9200088D0 (en) 1992-06-29
FI920058A0 (fi) 1992-01-07
JPH05500615A (ja) 1993-02-12
NO920116L (no) 1992-03-10
CN1057294A (zh) 1991-12-25
CA2063575A1 (en) 1991-11-11
WO1991017250A1 (en) 1991-11-14

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