CO6170374A2 - Rosa que contiene flavona y malvadina y metodo para la produccion de la misma - Google Patents

Rosa que contiene flavona y malvadina y metodo para la produccion de la misma

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Publication number
CO6170374A2
CO6170374A2 CO09145330A CO09145330A CO6170374A2 CO 6170374 A2 CO6170374 A2 CO 6170374A2 CO 09145330 A CO09145330 A CO 09145330A CO 09145330 A CO09145330 A CO 09145330A CO 6170374 A2 CO6170374 A2 CO 6170374A2
Authority
CO
Colombia
Prior art keywords
gene
flavone
flavona
synthase gene
scrophulariaceae
Prior art date
Application number
CO09145330A
Other languages
English (en)
Inventor
Yoshikazu Tanaka
Yukihisa Katsumoto
Yuko Fukui
Masako Mizutani
Noriko Nakamura
Junichi Togami
Original Assignee
Int Flower Dev Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Flower Dev Pty Ltd filed Critical Int Flower Dev Pty Ltd
Publication of CO6170374A2 publication Critical patent/CO6170374A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11022—Flavone synthase (1.14.11.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13088—Flavonoid 3',5'-hydroxylase (1.14.13.88)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La invención suministra una rosa caracterizada porque comprende una flavona y malvidina agregada mediante un método de modificación genética. La flavona y malvidina son típicamente producidas mediante la expresión de un gen de flavona sintasa transferido, un gen flavonoide 3', 5'-hidroxilasa de pensamiento y un gen de antocianina metiltransferasa. El gen de flavona sintasa es, por ejemplo un gen de flavona sintasa de la familia Scrophulariaceae y específicamente éste puede ser un gen de flavona sintasa de linaria de la familia Scrophulariaceae, o el gen de flavona sintasa de torenia de la familia Scrophulriaceae. El gen flavonoide 3', 5-hidroxilasa es, por ejemplo, el gen flavonoide 3',5'-hidroxilasa de pensamiento. El gen de antocianina metiltransferasa es, por ejemplo, el gen de metiltransferasa de torenia de la familia Scrophulariaceae. 1.- Una rosa caracterizada porque comprende una flavona y una malvidina agregada mediante un método de modificación genética. 2.- Una rosa de acuerdo a la reivindicación 1, que comprende una flavona y una malvidina mediante la expresión del flavonoide 3', 5'-hidroxilasa y antocianina metiltransferasa de pensamiento (Viola x wittrockiana). 3.- Una rosa de acuerdo con la reivindicación 1 ó 2 que comprende malvidina, una flavona de delfinidina mediante la expresión de un gen de antocianina metiltransferasa, un gen de flavona sintasa y el gen flavonoide 3', 5'-hidroxilasa de pensamiento (Viola x wittrockiana). 4.- Una rosa de acuerdo a una cualquiera de las reivindicaciones 1 a 3, en donde el gen de flavona sintasa es un gen de flavona sintasa derivado de la familia Scrophulariaceae. 5.- Una rosa de acuerdo a la reivindicación 4, en donde el gen de flavona sintasa derivado de la familia Scrophulariaceae es un gen de flavona sintasa derivado de linaria de la familia Scrophulariaceae (Scrophulariaceae, Antirrhinum majus). 6.- Una rosa de acuerdo a la reivindicación 4, en donde el gen de flavona sintasa derivado de la familia Scrophulariaceae es un gen de flavona sintasa derivado de torenia de la familia Scrophulariaceae (Scrophulariaceae, Torenia hybrida).
CO09145330A 2007-06-20 2009-12-18 Rosa que contiene flavona y malvadina y metodo para la produccion de la misma CO6170374A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007162959 2007-06-20

Publications (1)

Publication Number Publication Date
CO6170374A2 true CO6170374A2 (es) 2010-06-18

Family

ID=40156355

Family Applications (1)

Application Number Title Priority Date Filing Date
CO09145330A CO6170374A2 (es) 2007-06-20 2009-12-18 Rosa que contiene flavona y malvadina y metodo para la produccion de la misma

Country Status (15)

Country Link
US (1) US20100281575A1 (es)
EP (1) EP2163619B1 (es)
JP (1) JP5057349B2 (es)
KR (1) KR101168421B1 (es)
CN (1) CN101679972B (es)
AU (1) AU2008264434B2 (es)
BR (1) BRPI0813127A8 (es)
CA (1) CA2690754C (es)
CO (1) CO6170374A2 (es)
EC (1) ECSP099814A (es)
ES (1) ES2385416T3 (es)
IL (1) IL202818A (es)
RU (1) RU2463348C2 (es)
TW (1) TWI440430B (es)
WO (1) WO2008156206A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2691156A1 (en) * 2007-06-20 2008-12-24 International Flower Developments Proprietary Limited Rose containing flavone and delphinidin, and method for production thereof
JPWO2008156211A1 (ja) * 2007-06-20 2010-08-26 インターナショナル フラワー ディベロプメンツ プロプライアタリー リミティド フラボンを含むバラ及びその生産方法
KR101659525B1 (ko) * 2009-03-27 2016-09-23 산토리 홀딩스 가부시키가이샤 파란 장미에 함유되는 신규 화합물
CA2759261A1 (en) 2009-04-24 2010-10-28 Suntory Holdings Limited Method for production of chrysanthemum plant having petals containing modified anthocyanin
CN105349553B (zh) * 2015-12-17 2018-09-07 海南大学 一种花色调节基因VwMYB29及其应用
CN108777947B (zh) * 2016-03-31 2022-11-11 三得利控股株式会社 具有蓝色系花色的植物及其制作方法
CN106754989B (zh) * 2016-12-21 2020-04-21 广东药科大学 布渣叶黄烷酮-2-羟基化酶及其编码基因与应用
CA3078387A1 (en) * 2017-10-03 2019-04-11 Suntory Holdings Limited Transformed plant having blue flower color, and method for creating same
KR102010833B1 (ko) * 2017-11-08 2019-08-16 대한민국 화훼식물의 꽃잎, 암술기관 및 화심 색 변형 기능 매발톱꽃 유래 신규 유전자 및 이의 용도
JP7405836B2 (ja) * 2019-03-29 2023-12-26 サントリーホールディングス株式会社 フラボン７－ｏ－メチル基転移酵素遺伝子及びその使用
EP4039804A4 (en) * 2019-10-01 2024-01-24 Suntory Holdings Limited BUCKWHEAT C-GLYCOSYL TRANSFERASE GENE AND USE THEREOF
WO2022107857A1 (ja) 2020-11-18 2022-05-27 サントリーホールディングス株式会社 フラボン４'－ｏ－メチル基転移酵素遺伝子及びその使用
CN115094066B (zh) * 2022-05-06 2023-11-14 上海师范大学 一种调控月季花瓣颜色的基因RcTTG1及其应用

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US5480789A (en) * 1991-04-01 1996-01-02 Florigene Europe B.V. Genetically transformed rose plants and methods for their production
US5948955A (en) * 1991-07-13 1999-09-07 International Flower Developments Pty Ltd Transgenic plants having altered anthocyann levels
JP3403196B2 (ja) * 1992-03-02 2003-05-06 協和醗酵工業株式会社 新規植物遺伝子
US20010007157A1 (en) * 1993-11-18 2001-07-05 Ebrahim Firoozabady Genetically transformed rose plants and methods for their production
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JPH0970290A (ja) * 1995-02-17 1997-03-18 Suntory Ltd アシル基転移活性を有する蛋白質をコードする遺伝子
AUPN298895A0 (en) * 1995-05-16 1995-06-08 International Flower Developments Pty Ltd Transgenic plants exhibiting altered flower colour and methods for producing same
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AUPS017402A0 (en) * 2002-01-25 2002-02-14 International Flower Developments Pty Ltd Genetic sequences and uses therefor
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WO2004020637A1 (en) * 2002-08-30 2004-03-11 International Flower Developments Pty. Ltd. Flavonoid 3',5'hydroxylase gene sequences and uses therefor
JP4378676B2 (ja) * 2003-06-03 2009-12-09 日本電気株式会社 電力制御方法および電力制御回路
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CA2691156A1 (en) * 2007-06-20 2008-12-24 International Flower Developments Proprietary Limited Rose containing flavone and delphinidin, and method for production thereof

Also Published As

Publication number Publication date
AU2008264434A1 (en) 2008-12-24
US20100281575A1 (en) 2010-11-04
IL202818A0 (en) 2011-08-01
CA2690754A1 (en) 2008-12-24
CA2690754C (en) 2014-09-23
JPWO2008156206A1 (ja) 2010-08-26
CN101679972A (zh) 2010-03-24
IL202818A (en) 2015-08-31
WO2008156206A1 (ja) 2008-12-24
ECSP099814A (es) 2010-02-26
RU2010101626A (ru) 2011-07-27
AU2008264434B2 (en) 2012-03-08
BRPI0813127A8 (pt) 2016-06-07
TWI440430B (zh) 2014-06-11
EP2163619A1 (en) 2010-03-17
KR20100017986A (ko) 2010-02-16
JP5057349B2 (ja) 2012-10-24
TW200911109A (en) 2009-03-16
ES2385416T3 (es) 2012-07-24
RU2463348C2 (ru) 2012-10-10
EP2163619B1 (en) 2012-05-16
CN101679972B (zh) 2013-09-04
BRPI0813127A2 (pt) 2014-10-14
EP2163619A4 (en) 2010-11-03
KR101168421B1 (ko) 2012-07-25

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